• Bulletin of the Korean Chemical Society
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• v.32 no.5
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• pp.1569-1574
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• 2011

The presence of replication-competent adenovirus (RCA) subpopulations in adenoviral vector products raises a variety of safety issues for development of therapies based on gene therapy. To analyze the differing effects of adenoviral vector and RCA in vivo, we examined alterations in amino acids (AAs) using rat plasma following injection of ${\beta}$-galactosidase expressing recombinant adenovirus (designated rAdLacZ) or RCA. Plasma AAs were examined by gas chromatography-mass spectrometry. A total of 16 AAs were positively measured. In the rAdLacZ group compared to the control group, the level of aspartic acid was significantly increased (Student's t-test), while the level of glutamic acid was significantly reduced. Additionally, in the RCA group compared to the control group, the level of four AAs, valine, leucine, and isoleucine as branched-chain amino acids, and proline were significantly increased, whereas the levels of three AAs, glycine, threonine, and glutamic acid were significantly reduced. Altered plasma free AA metabolic patterns in rAdLacZ and RCA groups, compared with the control group, may explain the disturbance of AA metabolism related to viral infection.

• Journal of Microbiology
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• v.34 no.1
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• pp.37-37
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• 1996

The internal regions of nuclear small subunit rRNA from 6 plaeurotus species and 5 Pleurotus ostreatus strains were amplified by PCR and sequenced. The DNA sequences of 8 Pleurotus strains (P. ostreatus NFFA2, NFFA4501, NFFA4001, KFFA4001, KFCC11635, P florida, P. florida, P. sajor-cuju, P. pulmonarius, and P. spodoleucus) were idential, but P. cornucopiae differed from them in two bases out of 605 bases. However, p[hylogenetic analysis of the sequences by DNA-distance matrix and UPGMA methods showed that P. ostreatus NFFA2m1 and NFFA2m2, known as mutants of P. ostreatus NFFA2, belonged to anther group of Basidiomycotina, which is close to the genus Auricularia. The difference of the SSU rDNA sequences of P. cornucopiae from other Pleurotus species tested corresponds to the difference of mitochondrial plasmid type present in Pleurotus species as observed by Kim et al. (1993, Korean J. Microbiol. 31, 141-147).ishement of silencing at the HMR/hsp82 locus can occur in G1-arrested cells. Cell cycle arrest at G1 phase was achieved by treatment of early log a cell cultures with .alpha.-factor mating pheromone, which induces G1 arrest. The result suggests that passage through S phase (and therefore DNA replication) is nor required for re-establishing silencer-mediated repression at the HMNRa/HSP82 locus. Finally, to test whether de nono protein synthesis is required for re-establishment of silencer-mediated repression, cells were pretreated with cycloheximide (500 /.mu.g/ml) 120 min. It was apparent that inhibiting protein synthesis delays, but does not prevent, re-establishment of silencer-mediated repression. Altogether, these results indicate that re-establishment of silencer-mediated repression is not dependent on the DNA replication and has no requirement for protein synthesis.

• Journal of KIISE:Computer Systems and Theory
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• v.33 no.10
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• pp.746-758
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• 2006

Many objects existing on wide area environments have the replication characteristics according to how to categorize using their own names or properties. From the clients' requests, the existing naming and trading services have not supported with the binding service for replicated solver object with the same service type. For this reason, we present an integrated model that can support the selection of replicated object and dynamic binding services on wide-area computing environments. This model suggests provides not only location management of replicated objects but also active binding service which enables to select a least-loaded object on the system to keep the balance of load between systems. In this purpose, constructing both the service plan and model for support solver object's binding with replication property on wide area computing environments has been researched. In this paper, we showed the test environment and analyzed the performance evaluation of client/server binding procedures via integrated binding service in federation model and verified our model under the condition to see whether load balance can be applied to our model. For the performance evaluation of suggested wide area integrated binding service federation model, evaluated the integrated binding service of each domain and analyzed the performance evaluation of process for non-replication object's under federation model environment. Also, we analyzed the performance evaluation of the federation model between domains for wide area environment. From the execution results, we showed the federation model provides lowers search-cost on the physical tree structure of network.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2003.10a
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• pp.371-376
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• 2003

The hot embossing process has been mentioned as one of major nanoreplication techniques. This is due to its simple process, low cost, high replication fidelity and relatively high throughput. As the initial step of quantitating the embossing process, simple parametric study about embossing time have been carried out using high-resolution masters which patterned by the DRIE process and laser machining. Under the various embossing time, the viscous flow of thin PMMA films into microcavities during Compression force has been investigated. Also, a study about simulating the viscous flow during embossing process has planned and continuum scale FDM analysis was applied on this simulation. With currently available test data and condition, simple FDM analysis using FLOW3D was made attempt to match simulation and experiment.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.1036-1042
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• 2003

The hot embossing process has been mentioned as one of major nanoreplication techniques. This is due to its simple process, low cost, high replication fidelity and relatively high throughput. As the initial step of quantitating the embossing process , simple parametric study about embossing time have been carried out using high-resolution masters which patterned by the DRIE process and laser machining. Under the various embossing time, the viscous flow of thin PMMA films into microcavities during compression force has been investigated. Also, a study about simulating the viscous flow during embossing process has planned and continuum scale FDM analysis was applied on this simulation. With currently available test data and condition, simple FDM analysis using FLOW3D was made attempt to match simulation and experiment.

• Molecules and Cells
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• v.28 no.4
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• pp.403-406
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• 2009

Rev is an essential regulatory protein for HIV-1 replication. Rev (11-20) is known as the significant region regarding the function of a nuclear entry inhibitory signal (NIS) of Rev. In this study, anticandidal effects and mechanism of action of Rev (11-20) were investigated. The result exhibited that Rev (11-20) contained candidacidal activities. To understand target site(s) of Rev (11-20), the intracellular localization of the peptide was investigated. The result showed that Rev (11-20) rapidly accumulated in the fungal cell surface. The cell wall regeneration test also indicated that Rev (11-20) exerted its anticandidal activity to fungal plasma membrane rather than cell wall. The fluorescent study using 1,6-diphenyl-1,3,5-hexatriene (DPH) further confirmed the membrane-disruption mechanism(s) of Rev (11-20). The present study suggests that Rev (11-20) possesses significant potential regarding therapeutic agents for treating fungal diseases caused by Candida species in humans.

• Korean Journal of Microbiology
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• v.25 no.3
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• pp.165-172
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• 1987

An expression vector in a mammalian cell was constructed using the origin of replication (OR) and the promoters of SV40. The plasmid pSVOE was constructed by inserting SV40 DNA fragment (1, 118bp) containing SV40 OR and promoters into pBR322-1, and then a multiple cloning sequence was inserted at the immediate downstream of the late promoter of SV40 in the pSVOE vector. The plasmid was named pSVML. As a selection marker, thymidine kinase gene of herpes simplex virus with its promoter was inserted into EcoRI site of pSVML and the recombinant was named pSVML-TKp. To test the expression capacity of foreigen gene inserted at the multiple cloning site of pSVML, the thymidine kinase gene without its own promoter was inserted at the BamHI site of pSVML. The recombinant was named pSVML-TK. These plasmids, pSVML-TKp and pSVML-TK, were transfected into COS cells with calcium phosphate precipitation method. The thymidine kinase activity was significantly increased in both transfected cells.

• Journal of the Korean Wood Science and Technology
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• v.26 no.4
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• pp.92-97
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• 1998

Four urea-formaldehyde (UF) resins were synthesized as a particleboard (PB) binder with the four different initial formaldehyde/urea mole ratio and the final mole ratio of 1.15. The UF resins were characterized according to the standard method of resin adhesive analysis. PBs were manufactured using liquid UF resins at 5 minutes press time and 6% resin solids levels on an ovendry particle weight basis. A total of 20 PBs was fabricated for 5 panel replication per UF resin types. The panels were tested for physical strength properties per the procedure ASTM D 1037. The formaldehyde emission levels from the PBs bonded with the UF resins were tested according to 2-hour desiccator test method ASTM D 5582. There were no significant differences among UF resin types for internal bond strength of PBs. But there were significant differences among UF resin types for formaldehyde emission level of PBs. The results showed that the formaldehyde emission level was influenced by the UF resin types without reducing the adhesive performance.

• The Journal of Internal Korean Medicine
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• v.41 no.3
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• pp.424-433
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• 2020

Qingfei Paidu decoction is recommended for treatment and prevention of COVID-19. It is a prescription that combines Mahaenggamseok-tang, Oryeong-san, Soshiho-tang, Saganmahwang-tang, and Kwakhyangjeonggi-san. According to several clinical reviews, symptoms of COVID-19 were improved, laboratory test indicators were improved, and the cure rate was increased. Pharmacologically, the Qingfei Paidu decoction and its composition have been reported to inhibit viral replication, alleviate immune responses, and have anti-inflammatory and antiviral effects. Severe side effects were not revealed, and this treatment is considered relatively safe. However, to validate and better understand these findings, well-designed clinical studies are needed.

• Journal of the Korea Safety Management & Science
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• v.18 no.4
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• pp.179-183
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• 2016

The purposes of this research are to modelize test system of SM TESTING by ARENA, software, input several items of specimen's testing process, resources of system and transfer loop, etc, give a hypothesis and then, obtain results reducing the efficiency of the whole system finally by overload of specific facilities in the testing system through the simulation so as to obtain several materials such as specimen and testing facility, transfer loop, etc. by simple and various forms without any necessity of numerical modelization. It will add facilities of over load and reduce facilities with low operation rate, so increase the efficiency of the system.