• Title/Summary/Keyword: Replication Ratio

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Effect of Photoperiod on the Growth and Matter Production in Zoysia matrella (일조시간이 금잔디(Zoysia matrella)의 생장 및 물질생산에 미치는 영향)

  • 도봉현
    • Asian Journal of Turfgrass Science
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    • v.3 no.2
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    • pp.83-88
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    • 1989
  • This Study was designed to know the growth Status under various photoperiod using Zoysia matrella. The experimental plots were split into such groups as control (full sunlight) 3hour, 6hour and 9hour photoperiod by three replication of completely randomized design. Chlorophyll content was analyized by mackine method and total soluble sugar by anthrone method. The result obtained from this experiment was as fol1 lows ; l. Dry weight was high in the control and 9hour plots. Its decrease remarkably occured in the short day plots 2. Leaf growth woes remarkaly decreased by short day condition. Six hour was considered as critical duration for leaf growth. 3. The growth of rhizome in the 9hour and control plots was good. New development of rhizome in the' short day plots was very slow compare to the control plot. 4. Chlorophyll content was high at l8 days after transplanting and then decreased. Its content in the short day plots severely decreased. 5. The content of soluble sugar in the control was low at early growth stage and high at late growth stage. It was found that the shorter the exposure time to sunlight, the higher the ratio of sugar content.

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Nano Molding Technology for Optical Storage Media with Large-area Nano-pattern (대면적 광 정보저장매체의 나노성형에 대한 기술 개발)

  • Shin Hong-Gue;Ban Jun-Ho;Cho Ki-Chul;Kim Heon-Yong;Kim Byeong-Hee
    • Journal of the Korean Society for Precision Engineering
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    • v.23 no.4 s.181
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    • pp.162-167
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    • 2006
  • Hot embossing lithography(HEL) has the production advantage of comparatively few process step, simple operation, a relatively low cost for embossing tools(Si), and high replication accuracy for small features. In this paper, we considered the nano-molding characteristic according to molding parameters(temperature, pressure, times, etc) and induced a optimal molding condition using HEL. High precision nano-patter master with various shapes were designed and manufactured using the DRIE(Deep Reactive ion Etching), LPCVD(Low Pressure Chemical Vapor Deposition) and thermal oxidation process, and we investigated the molding characteristic of DVD and Blu-ray nickel stamp. We induced flow behaviors of polymer, rheology by shapes and sizes of the pattern through various molding experiments. Finally, with achieving nano-structure molding with high aspect ratio, we will secure a basic technology about the molding of large-area nano-pattern media.

Performance Evaluation of WAN Storage Migration Scheme for Cloud Computing Environment (클라우드 컴퓨팅 환경을 위한 WAN 스토리지 이주 기법 성능평가)

  • Chang, Jun-Hyub;Lee, Won-Joo;Jeon, Chang-Ho
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.5
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    • pp.1-7
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    • 2012
  • In this paper, we design and implement the simulator for WAN storage replication model performance evaluation in cloud computing environment. Each cloud of simulator is composed of virtual machine emulator and storage emulator. The virtual machine emulator is composed of read/write ratio module, the read/write sequence combination module, and the read/write request module. The storage emulator is composed of storage management module, data transfer module, read/write operations module, and overhead processing module. Using the simulator, we evaluate performance of migration scheme, pre-copy and the post-copy, considering about read/write ratio, network delay, and network bandwidth. Through simulation, we have confirmed that the average migration time of pre-copy was decreased proportional to the read operation. However, average migration time of post-copy was on the increase. Also, the average migration time of post-copy was increased proportional to the network delay. However, average migration time of pre-copy was shown uniformly. Therefore, we show that pre-copy model more effective to reduce the average migration time than the post-copy model. The average migration time of pre-copy and post-copy were not affected by the change of network bandwidth. Therefore, these results show that selects the storage replication model to be, the network bandwidth know not being the important element.

Control of X Chromosome Reactivation and Determination of the Ratio of Sex Chromosome to Autosome in Embryonal Carcinoma Cell-Somatic Cell Hybrids (배종양 세포와 체세포 간의 융합 세포에서 X 염색체 재활성화의 조절과 성염색체에 대한 상염색체 비율의 결정)

  • 이광호
    • The Korean Journal of Zoology
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    • v.39 no.1
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    • pp.75-88
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    • 1996
  • OTF9-63 (OTF9) and P19S1O1A1 (P19) embryonal carcinoma (EC) cells were examined for their ability to produce the readivation of inactive X chromosomes from somatic cells. They were hybridized with various somatic cells and resulting HATr EC-somatic cell clones were analysed for their morphology, chromosomal replication pafterns and expression proffies of X-linked and distantiy located genes, Hprt and Pgk-1. The results demonstrated that 0RF9 cells could reactivate the inactive X chromosome whereas P19 cells could not. In adition, EC-somatic cell hybrids tended to reduce the number of sex chromosomes in long-term culture, resulting m 1:2 ratio of sex chromosomes to autosomes The use of EC cell hybrids provides an experimental system for studying the mechanism(s) of the X-reactivatio that is initiated and maintained from meiotic prophase of oogenesis to early embryogenesis.

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Influence of Compost Recycling and Magnesium Supplement on Physical and Chemical Traits of Animal Manure Compost

  • Lee, Jin-Eui;Kwag, Jung-Hoon;Ra, Chang-Six
    • Journal of Animal Science and Technology
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    • v.52 no.6
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    • pp.513-519
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    • 2010
  • A series of experiments were performed to study the influence of the following parameters on the physical traits and composition of swine manure compost: (1) addition of magnesium (Mg) at a molar ratio of 1.2 with respect to $PO_4$, and (2) reutilization of compost containing $MgNH_4PO_4{\cdot}6H_2O$ (magnesium ammonium phosphate, MAP). Three independent batch tests were conducted for replication: batch test I-control (C) and Mg added (T), batch test II-C, T and compost recycle ($T_{R1}$), and batch test III-C, T and compost recycle ($T_{R2}$). Magnesium addition and compost reutilization had no adverse effect on the degradation of organic matter. Reuse of the compost, however, had a clear effect on the total nitrogen (TN) and total phosphorus (TP) contents in the final compost. Repeated compost reutilization as a bulking material was resulted in composts rich in N and P. Upon adding the Mg supplement to the composting materials, the ortho-phosphate (OP) to TP ratio decreased due to the MAP crystallization reaction. The decrease in the OP/TP ratio and the increase in the TP content of the compost indicate that water-soluble phosphate is converted into a slow-release phosphate by the formation of crystals during composting. X-ray diffraction analysis of the irregular shaped crystals in the compost indicated that they are MAP crystals and that the crystallization of MAP begins immediately after the addition of the Mg supplement. The Mg addition to composting materials and the reutilization of compost as a bulking material would be a practical means to conserve nutrient content.

Effect of Intercropping Ratio on the Cherry Tomato with Basil on the Growth, Physiological, and Productivity Parameters on the Rooftop in Urban Agriculture (옥상 도시농업에서 방울토마토(Lycopersicon esculentum)와 바질(Ocimum basilicum)간의 공영식재가 생육, 생리, 생산성에 미치는 영향)

  • Ju, Jin-Hee;Song, Hee-Yeon;Oh, Deuk-Kyun;Park, Sun-Yeong;Yoon, Yong-Han
    • Journal of Environmental Science International
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    • v.30 no.9
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    • pp.709-717
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    • 2021
  • This study evaluated the growth, physiological responses and productivity based on the intercropping ratio of cherry tomato (Lycopersicon esculentum L.) with basil (Ocimum basilicum L.). on the rooftops to determine out the efficient ratio in urban agriculture. From April to September 2019, an experiment was conducted on the rooftop of Konkuk University Glocal Campus. Cherry tomato and basil were selected as companion plants for eco-friendly urban agriculture on the rooftops. Each plot was created with a width of 100 cm, length of 100 cm, and height of 25 cm. After installing drainage and waterproof layers from bottom to top, substrate was laid out with a height of 20 cm. Intercropping ratio was consisted of a single tomato plant (TC), 2:1 tomato to basil (T2B1), 1:1 tomato to basil (T1B1), 1:2 tomato to basil 2 (T1B2), and a single basil plant (BC), were conducted using a randomized complete plot design with five treatments and three replication (a total 15 plots). Measurements were divided into growth, physiological responses, and productivity parameters, and detailed items were investigated and analyzed by classifying them into plant height, leaf length, leaf width, number of leaves, root length, root collar caliper, chlorophyll contents, fresh weight, dry weight, number of fruit, fruit caliper, fruit weight, and sugar content. Comparative analyses of cherry tomato with basil plants by intercropping ratio, growth, physiological, and productivity responses are determined to be efficient when the ratio of cherry tomato to basil ratio is 2:1 or 1:1.

Effects on the process factors of blow molding affects to the PET bottle (사출성형 특성을 고려한 2인치 휴대폰용 도광판 금형제작에 관한 연구)

  • Hwang, Chul-Jin;Do, Young-Soo;Kim, Jong-Sun;Min, In-Gi;Kim, Jong-Dug;Yoon, Kyung-Hwan
    • Design & Manufacturing
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    • v.2 no.6
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    • pp.1-6
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    • 2008
  • Recently, many researches have been done to improve optical performance of LCD-BLU(Back Light Unit). One of the most important parts in LCD-BLU is LGP(Light Guiding Plate). Micro-patterned LGP is known to have different optical characteristics depending on their shape, pattern density and size, etc. In the present study, a micro-optical patterned LGP mold was fabricated using LiGA process. The difference in the optical characteristics between positive and negative patterned LGP's was investigated by fixing the density, location and size of each pattern. It was found that the negative patterned LGP showed better optical characteristics than positive one.

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Nucleotide Insertion Fidelity of Human Hepatitis B Viral Polymerase

  • Kim, Youn-Hee;Hong, Young-Bin;Suh, Se-Won;Jung, Gu-Hung
    • BMB Reports
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    • v.33 no.2
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    • pp.126-132
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    • 2000
  • The hepadnaviruses replicate their nucleic acid through a reverse transcription step. The MBP-fused HBV polymerase was expressed in E. coli and purified by using amylase affinity column chromatography. The purified protein represented DNA-dependent DNA polymerase activity. In this report, the MBP-HBV polymerase was shown to lack 3'$\rightarrow$5' exonuclease activity, like other retroviral RTs. The ratio of the insertion efficiency for the wrong versus right vase pairs indicates the misinsertion frequency (f). The nucleotide insertion fidelity (1/f), observed with the MBP-HBV polymerase and HIV-1 RT, was between 60 and 54,000, and between 50 and 73,000, respectively, showing that they are in close range. A relatively efficient nucleotide incorporation by the MBP-HBV polymerase was observed with a specificity of three groups: (1) A : T, T : A>C : G, G : C (matched pairs), (2) A : C, C : A>G: T, T : G (purine-pyrimidine and pyrimidine-purine mispairs), and (3) C : C, A : A, G : G, T : T>T : C, C : T>A : G, G : A (purine-purine or pyrimidine-pyrimidine mispairs), and their order is (1)>(2)>(3). The data from the nucleotide insertion fidelity by the MBP-HBV polymerase suggest that the HBV polymerase may be as error-prone as HIV-1 RT.

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Possible Mechanism Underlying the Antiherpetic Activity of a Proteoglycan Isolated from the Mycelia of Ganoderma lucidum in Vitro

  • Li, Zubing;Liu, Jing;Zhao, Yifang
    • BMB Reports
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    • v.38 no.1
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    • pp.34-40
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    • 2005
  • GLPG (Ganoderma lucidum proteoglycan) was a bioactive fraction obtained by the liquid fermentation of the mycelia of Ganoderma lucidum, EtOH precipitation, and DEAE-cellulose column chromatography. GLPG was a proteoglycan with a carbohydrate: protein ratio of 10.4: 1. Its antiviral activities against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) were investigated using a cytopathic inhibition assay. GLPG inhibited cell death in a dose-dependent manner in HSV-infected cells. In addition, it had no cytotoxic effect even at 2 mg/ml. In order to study the mode of action of the antiviral activity of GLPG, cells were treated with GLPG before, during, and after infection, and viral titer in the supernatant of cell culture 48 h post-infection was determined using a $TCID_{50}$ assay. The antiviral effects of GLPG were more remarkable before viral treatment than after treatment. Although the precise mechanism has yet to be defined, our work suggests that GLPG inhibits viral replication by interfering with the early events of viral adsorption and entry into target cells. Thus, this proteoglycan appears to be a candidate anti-HSV agent.

A Newly Isolated Bacteriophage, PBES 02, Infecting Cronobacter sakazakii

  • Lee, Hyung Ju;Kim, Wan Il;Kwon, Young Chan;Cha, Kyung Eun;Kim, Minjin;Myung, Heejoon
    • Journal of Microbiology and Biotechnology
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    • v.26 no.9
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    • pp.1629-1635
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    • 2016
  • A novel bacteriophage, PBES 02, infecting Cronobacter sakazakii was isolated and characterized. It has a spherical head of 90 nm in diameter and a tail of 130 nm in length, and belongs to Myoviridae as observed under a transmission electron microscope. The major virion protein appears to be 38 kilodaltons (kDa) in size. The latent period of PBES 02 is 30 min and the burst size is 250. Infectivity of the phage remained intact after exposure to temperatures ranging from 4℃ to 55℃ for 1 h. It was also stable after exposure to pHs ranging from 6 to 10 for 1 h. The phage effectively removed contaminating Cronobacter sakazakii from broth infant formula. PBES 02 has a double-stranded DNA genome of 149,732 bases. Its GC ratio is 50.7%. Sequence analysis revealed that PBES 02 has 299 open reading frames (ORFs) and 14 tRNA genes. Thirty-nine ORFs were annotated, including 24 related to replication and regulation functions, 10 related to structural proteins, and 5 related to DNA packaging. The genome of PBES 02 is closely related to that of two other C. sakazakii phages, CR3 and CR8. Comparison of DNA sequences of genes encoding the major capsid protein revealed a wide geographical distribution of related phages over Asia, Europe, and America.