• Korean Journal of Head & Neck Oncology
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• v.22 no.2
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• pp.130-136
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• 2006

Introduction : The sensitivity of tumor cells to radiotherapy is a critical determinant of local control and potential cure in advanced head and neck squamous cell carcinoma(HNSCC). The emergence of radioresistant tumor cells is an obstacle to cancer therapy. Most radioresistant cells have a higher proportion of cells in the Sphase of the cell cycle and a lower apoptotic fraction than radiosensitive cells. HSV replication is increased in cells that have higher S-phase fractions. NV1066 is an oncolytic herpes simplex virus type-1 mutant. We hypothesized that NV1066 replication and cytotoxicity are increased in radioresistant cells. The purpose of this study is to evaluate the antitumor efficacy of NV1066 to treat radioresistant HNSCC. Methods : Radioresistant cells were selected by treating five HNSCC cell lines with repeated conventional fractionated doses of radiation(2Gy/day), using a Cs-137 irradiator, up to a cumulative dose of 70Gy. Clonogenic cell survival and S-phase fractions were compared between radioresistant and parental radiosensitive cells. The two cell populations were then treated with NV1066 to examine viral replication, by the viral plaque assay and viral cytotoxicity. Results : Fractionated irradiation resulted in the selection of radioresistant cells. Radioresistant cells had a higher S-phase fraction(42.9%) compared to parental cells(26.2%). NV1066 replication in radioresistant cells was 7.4 times higher than in parental cells(p<0.01). Treatment with NV1066 resulted in increased cytotoxicity of 24.5% in radioresistant cells compared to parental cells(p<0.05). Conclusion : NV1066 showed increased viral replication and cytotoxicity in radioresistant HNSCC cell lines. These findings suggest a potential clinical application for this oncolytic viral therapy as treatment for radioresistant head and neck cancers.

• The Transactions of the Korea Information Processing Society
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• v.3 no.3
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• pp.631-639
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• 1996

In this paper we discuss the elements which must be considered for collaboration- aware application design and their effcets on user interfaces. Collaboration-aware applications have inter-user and user/system interaction features besides generic real-time distribution system features and this restricts the design freedom with the requirement of consistent user interfaces. Programmers, therefore, must provide uniform and logically consistent user interfaces to user and, for this end, they should consider main design features such as concurrency control algorithms, system topology and object replication scheme. Among the design factors concurrency control algorithms have relatively significant impact on user interfaces and we consider the impact of concurrency control agorithms along with that of thesystem to pologyand the objectreplication scheme. Coshed/SAS, a groupeditor, is given as an example of application of the factors.

• Korean Journal of Child Studies
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• v.30 no.2
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• pp.211-229
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• 2009

This study examined replication of the factor structure of the DAPC [Domain-specific Assessment of Psychological Control (Soenens et al., 2006)] in parents of Korean adolescents. Participants included 289 adolescents who responded to DAPC items regarding their parents' psychological control behaviors. Two factors comprising 17 items were identified and labeled as "achievement oriented psychological control", and "separation-anxious psychological control." Confirmatory factor analysis and partial correlation analysis provided evidence for the construct validity of the DAPC through construct reliability, convergent validity and discriminant validity across both sex of parents and adolescents. The psychometric properties indicated that DAPC could be useful in studying parents' psychological control behaviors toward their adolescent children.

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.96-102
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• 2002

The S-phase checkpoint mechanisms response to DNA damage or inhibition of DNA replication for maintenance of genetic stability in eukaryotic cells. These roles include cell cycle control arrest at S-phase and Iranscriptional induction of repair genes. To characterize the defects of dpbll mutant for both these responses, we examined the over-expression effect of DPBll gene, the sensitivity to HU, MMS, and the transcriptional pattern by DNA damage agent for RNRS mRNA. RNRS transcript is induced in response to a wide variety of agents that either damage D7A directly through chemical modification or induce stress by blocking DNA synthesis. As results, dpbll-1 cells are sensitive to DNA damage agents and the level of RNR3 mRNA is reduced approximately 40% than wild type cells. Moreover, we found the same results in dpb2-1 cells. Therefore, we propose that DPB2 and DPBll act as a sensor of replication that coordinates the transcriptional and cell cycle responses to replication blocks.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.2012-2018
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• 2016

Coxsackievirus B3 (CVB3) is the main cause of acute myocarditis and dilated cardiomyopathy. Plant extracts are considered as useful materials to develop new antiviral drugs. We had previously selected candidate plant extracts, which showed anti-inflammatory effects. We examined the antiviral effects by using a HeLa cell survival assay. Among these extracts, we chose the Amomi Cardamomi (Amomi) extract, which showed strong antiviral effect and preserved cell survival in CVB3 infection. We investigated the mechanisms underlying the ability of Amomi extract to inhibit CVB3 infection and replication. HeLa cells were infected by CVB3 with or without Amomi extract. Erk and Akt activities, and their correlation with virus replication were observed. Live virus titers in cell supernatants and viral positive- and negative-strand RNA amplification were measured. Amomi extract significantly increased HeLa cell survival in different concentrations ($100-10{\mu}g/ml$). CVB3 capsid protein VP1 expression (76%) and viral protease 2A-induced eIF4G1 cleavage (70%) were significantly decreased in Amomi extract ($100{\mu}g/ml$) treated cells. The levels of positive- (20%) and negative-strand (80%) RNA were dramatically decreased compared with the control, as revealed by reverse transcription-PCR. In addition, Amomi extract improved mice survival (51% vs 26%) and dramatically reduced heart inflammation in a CVB3-induced myocarditis mouse model. These results suggested that Amomi extract significantly inhibited Enterovirus replication and myocarditis damage. Amomi may be developed as a therapeutic drug for Enterovirus.

• The KIPS Transactions:PartA
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• v.12A no.2 s.92
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• pp.171-180
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• 2005

In distributed systems data are replicated and stored at several nodes to increase the availability and overall performance. Here Quorum protocol doffing a certain set of replicas required for read/write operation exists for global concurrency control. One of the representative replication Protocols - the Tree Quorum protocol - has a drawback of rapidly growing number of replicas as the level increases, while the Grid protocol requires the same operation cost even without any failure. In this paper, thus, we propose a new replication protocol called hybrid protocol which capitalizes the merits of the existing protocols and solves the problems of them at the same time. The proposed hybrid protocol has very low operation cost in the absence of failure like the tree quorum protocol, and has relatively lower operation cost and higher availability than existing protocols when failure occurs by employing tree architecture as the overall organization while each level of the tree is organized as a row of a grid architecture. It is thus effective to be applied to survival storage system. We conduct cost and availability analysis of the proposed protocol through mathematical modeling, and response time and throughput are compared with those of the Tree Quorum protocol through computer simulation.

• Journal of the Korea Society of Computer and Information
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• v.26 no.4
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• pp.75-84
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• 2021

In this paper, we introduce InfoDID, a robust user information management service based on DID that manages user information reliably. Since blockchain technology provides an environment in which data can be handled transparently on a decentralized basis, various services using blockchain are currently being developed As the importance of user's personal information has recently emerged, the DID technology is receiving attention. The technology allows a user to control his or her information through decentralized identifiers, and various information management services are being tried based on the technology. Using blockchain-based DID technology, InfoDID reliably controls personal information requested frequently, helping users to provide their information to other services more conveniently. In addition, to support service continuity, InfoDID uses BR2K technique that provides robust execution of blockchain application services, so that even partial service failures can be systematically recovered. To facilitate this operation, we present a replication status monitoring tool that can continuously check the replication states of blockchain application services running in association with the BR2K technique such as InfoDID.

• Asian Journal of Turfgrass Science
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• v.3 no.2
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• pp.83-88
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• 1989

This Study was designed to know the growth Status under various photoperiod using Zoysia matrella. The experimental plots were split into such groups as control (full sunlight) 3hour, 6hour and 9hour photoperiod by three replication of completely randomized design. Chlorophyll content was analyized by mackine method and total soluble sugar by anthrone method. The result obtained from this experiment was as fol1 lows ; l. Dry weight was high in the control and 9hour plots. Its decrease remarkably occured in the short day plots 2. Leaf growth woes remarkaly decreased by short day condition. Six hour was considered as critical duration for leaf growth. 3. The growth of rhizome in the 9hour and control plots was good. New development of rhizome in the' short day plots was very slow compare to the control plot. 4. Chlorophyll content was high at l8 days after transplanting and then decreased. Its content in the short day plots severely decreased. 5. The content of soluble sugar in the control was low at early growth stage and high at late growth stage. It was found that the shorter the exposure time to sunlight, the higher the ratio of sugar content.

• Bulletin of the Korean Chemical Society
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• v.32 no.5
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• pp.1569-1574
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• 2011

The presence of replication-competent adenovirus (RCA) subpopulations in adenoviral vector products raises a variety of safety issues for development of therapies based on gene therapy. To analyze the differing effects of adenoviral vector and RCA in vivo, we examined alterations in amino acids (AAs) using rat plasma following injection of ${\beta}$-galactosidase expressing recombinant adenovirus (designated rAdLacZ) or RCA. Plasma AAs were examined by gas chromatography-mass spectrometry. A total of 16 AAs were positively measured. In the rAdLacZ group compared to the control group, the level of aspartic acid was significantly increased (Student's t-test), while the level of glutamic acid was significantly reduced. Additionally, in the RCA group compared to the control group, the level of four AAs, valine, leucine, and isoleucine as branched-chain amino acids, and proline were significantly increased, whereas the levels of three AAs, glycine, threonine, and glutamic acid were significantly reduced. Altered plasma free AA metabolic patterns in rAdLacZ and RCA groups, compared with the control group, may explain the disturbance of AA metabolism related to viral infection.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.5
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• pp.683-689
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• 2017

Objective: The experiments were carried out to measure the effect of multivitamin, acidifier and Azolla on growth performance, profitability and lipid profiles of blood of broiler chickens to produce safe and cost effective broilers. Methods: In experiment 1, 240 day-old Cobb-500 broiler chicks were fed diets; $D_1$ (control), $D_2$ ($D_1$ with 1 mL multivitamin/liter water), $D_3$ ($D_1$ with 1 mL acidifier/liter water), $D_4$ ($D_1$ with 1 mL multivitamin and 2 mL acidifier/liter water) having 3 replications in each, and 20 chicks/replication. In experiment 2, 150 day-old Cobb-500 broiler chicks were fed diets; $T_1$ (control), $T_2$ (5% Azolla in the diet), $T_3$ (7% Azolla in the diet) and $T_4$ ($T_1$ with 1 mL multivitamin and 1 mL acidifier/liter water) having 3 replications in each, and 20 chicks/replication in control, and 10 chicks/replication in the remaining dietary treatment groups for 35 days. Results: In experiment 1, the highest live weight was observed in $D_4$ (p<0.05), however, feed intake was statistically similar between diets (p>0.05). The lowest feed conversion ratio (FCR) (p<0.001) and mortality (p<0.05) were observed in $D_2$ followed by $D_4$, $D_1$, and $D_3$, respectively. There were no significant differences between diets for feed cost and net profit (p>0.05). However, evidently but not significantly, the highest net profit was obtained in $D_2$ followed by $D_4$, $D_1$, and $D_3$, respectively. In experiment 2, the highest live weight (p<0.05) and feed intake (p<0.001) were observed in $T_4$. Mortality (p<0.01), FCR (p<0.01), feed cost (p<0.05) and net profit (p<0.05) were significantly different among diets. Considering net profit, $T_2$ was the best performing dietary group followed by $T_3$, $T_1$, and $T_4$, respectively. The lowest lipid profiles were observed in $D_3$ followed by $D_1$, $D_4$, and $D_2$, respectively (p<0.05). In experiment 2, the lowest total cholesterol, TG, and the highest amount of high density lipoprotein were observed in $T_2$, followed by $T_3$, $T_1$, and $T_4$, respectively (p<0.05). Evidently but not significantly, low density lipoprotein was the highest in $T_2$ followed by $T_3$, $T_4$, and $T_1$, respectively (p>0.05). Conclusion: In conclusion, Azolla and acidifier reduced lipid profiles of broiler chickens. Considering net profit and lipid profiles, 5% Azolla may be the suitable dietary group for producing safe and profitable broilers. However, more studies are needed to confirm this study prior to suggesting using Azolla in the poultry industry.