• 한국소음진동공학회논문집
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• 제23권5호
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• pp.438-444
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• 2013

This paper presents the replication of a desired vibration response by iterative learning control (ILC) system for a vibration motion replication actuator. The vibration motion replication actuator has parameter uncertainties including system nonlinearity and joint nonlinearity. Vehicle manufacturers worldwide are increasingly relying on road simulation facilities that put simulated loads and stresses on vehicles and subassemblies in order to reduce development time. Road simulation algorithm is the key point of developing road simulation system. With the rapid progress of digital signal processing technology, more complex control algorithms including iterative learning control can be utilized. In this paper, ILC algorithm was utilized to produce simultaneously the six channels of desired responses using the Stewart platform composed of six linear electro-magnetic actuators with Halbach magnet array. The convergence rate and accuracy showed reasonable results to meet the requirement. It shows that the algorithm is acceptable to replicate multi-channel vibration responses.

• 대한기계학회논문집A
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• 제27권3호
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• pp.450-454
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• 2003

It is important to control the processing conditions to maximize the replication quality of UV-molded microstructure. In the present study, the tip radius anil surface roughness of V-groove structure were measured to quantify the replication quality. UV-curing dose and the applied pressure were experimentally selected as the governing Processing conditions that affect the replication quality of the UV-molded part. Finally. an experimental optimization technique combining central composite design and desirability function approach was used to maximize the replication quality of UV-molded structure.

• Journal of Biosystems Engineering
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• 제26권4호
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• pp.391-398
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• 2001

A robot system for clone replication and gridding, which is a preliminary state of the genome research, was developed and evaluated its performance. This gridding robot system consisted of 1) a gridding heat that replicated the clone, 2) a manipulator, as a part of body of robot, which transferred the gridding head along x-, y-, z-axis, 3) a well plate arranging board, 4) a sterilization unit, and 5) a control unit. Performance of the system was evaluated with 1) repeatability of the robot system, 2) clone replication efficiency, 3) time requirement of the replication, and 4) sterilization efficiency. The repeatability error of the robot system showed 0.219 mm and 0.094 mm in the direction of x- and y-axis, respectively. The success rate of the clone replication with the gridding head was 100% on the membrane filter. The time required for the replication was four minutes and fifty-five seconds from the four 96 well plates to a 384 well plate meanwhile the required time with well experienced hand labor was three minutes thirty-five seconds. The gridding operation of clone could not be done by hand labor and the required time with robot system for the gridding on the membrance filter with the control program 5$\times$5: 1 copy and 384 gridding pins was twenty minutes and twenty-five seconds. The efficiency of the sterilization was considered to be satisfactory since no growth of fungi was found around the area of replication in the membrane filter.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 춘계학술대회
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• pp.1078-1082
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• 2003

A feasibility study for the fabrication of master replication with nanostructures by Nanoimprint Lithography (NIL) was investigated for application of polymer Photonic Bandgap (PBG) devices used in photonic IC. Large area gratings of $9{\times}15(mm^2)$ with p = 400 nm was successfully embossed on PMMA on silicon wafer and the embossing parameters (temperature, pressure, time) were established. A precise control of $O_2$ plasma Reactive Ion Etching (RIE) process time allowed window opening over the whole area despite the presence of wafer bending. Master replication with aspect ratio 1 was successfully fabricated, but master replication with aspect ratio 3 needs to optimize parameters. All replications were done in a NIL process.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 춘계학술대회
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• pp.1332-1336
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• 2003

An experimental method is presented to maximize the replication quality of UV-molded micro-optical components. It is important to maximize the replication quality, because one can obtain the replicated micro-optical components with desired properties by accurate control of the shape. In the present study, a simple technique to avoid micro-air bubbles was first suggested. The effects of the UV-curing dose and the compression pressure on the replication quality of UV-molded structure were examined experimentally. Finally, as a practical application of the process design method, microlens arrays with diameters between 8 ${\mu}m$ and 96 ${\mu}m$ were fabricated by the present method, and the replication quality and the optical properties of the replicated microlens were measured and analyzed.

• 한국정보과학회논문지:정보통신
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• 제30권4호
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• pp.545-558
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• 2003

이동단말의 사용이 보편화 됨에 따라 통신 서비스의 개인화 및 맞춤화 요구가 증가하고 있다. 개인화 서비스의 궁극적인 목표는 사용자가 임의의 망으로 로밍해 가더라도 당초 가입한 서비스와 유사한 형태로(same look and feel) 서비스를 제공하는 가상 홈 환경의 실현에 있다. 가상 홈 환경에서는 개인의 서비스를 정의하는 서비스 프로파일의 관리가 필수 절차가 된다. 이러한 가상 흠 환경을 구현하기 위한 두 가지 방법은 가입자가 로밍하는 곳에 프로과일을 중복하여 방문망 내에서 서비스론 제공하는 무조건추종중복[Follow-Me Replication Unconditional(FMRU)] 기법과, 홈 망의 서비스시스템으로부터 서비스를 실행하는 중앙집중(Central) 기법으로, 전자는 call-to-mobiligy ratio(CMR)이 높은 가입자에게, 후자는 CMR이 낮은 가입자에게 적합하다. 그러나 해당 가입자가 어떤 경우에나 고정된 CMR을 갖는 것은 아니므로 평균적인 CMR로 위 두 기법을 선택하여 적용하는 것은 성능 저하의 문제가 있다. 본 논문에서는 가상 홈 환경에서 서비스 프로파일 관리를 위한 두 가지 기법을 구체화하고, FMRU를 개선한 요구기반 무조건추종중복[Follow-Me Replication Unconditional on-Demand(FMRUD)]기법을 제시하였다. 또한 가입자의 변화하는 CMR에 따라 자동적으로 두 기법을 선택하도록 하는 적응적 추종 중복[Adaptive Follow-Me Replication(AFMR)]기법을 제안하였으며 이들을 비교하였다. 반응 수식을 통한 성능분석 결과 프로파일 크기 관점에서 효율성을 판단하며 CMR 변화를 고려하는 AFMR이 더욱 높은성능을 가짐을 알 수 있다.

• Journal of Microbiology
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• 제33권3호
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• pp.191-195
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• 1995

IciA protein has been shown as an inhibitor for the initiation of E. coli chromosomal DNA replication at oriC. IciA protein binds the AT-rich region in oriC and then blocks the initiation of chromosomal DNA replication. Two binding sites for IciA protein were identified in dnaA gene, encoding the initiator for the E. coli chromosomal replication, promoter region by gel-shift assay and DNase I footprinting, One, named as IciA site I, is located upstream of the dnaA promoter 1P. The other, named as IciA site II, is located downstream of the dnaA promoter 2P. The sequence comparison of the regions protected from the DNase I cleavage did not result in a clear consensus sequence for the binding of IciA protein, suggesting that IciA protein may be a member of multimeric complex dsDNA binding proteins. This study provided information about the binding mode of IciA protein. Even though the IciA site II and IciA binding site in oriC seem to be composed of two IciA binding units, one binding unit is likely enough to cause the binding of IciA protein to the IciA site I. The binding of IciA protein to the dna4 promoter implies that IciA protein may involve not only the control of the initiation of chromosomal DNA replication but also the control of the dna4 gene expression.

• 미생물학회지
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• 제32권2호
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• pp.115-119
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• 1994

Staphylococcus aureus DH1에서 분리된 R-plasmid pSBK203상의 복제개시 인자인 rep 유전자산물의 발현이 어떻게 조절되는가를 밝히기 위해 관련 부위를 확인하고 cloning한 후 그 염기서열을 결정하였으며 이를 같은 계열에 속하는 pT181족 plasmid들의 서열과 그 상동성을 비교 분석하였다. 복제 조절 관련 부위에 염기 삽입 및 염기 결손을 유도함으로써 얻어진 변이체들의 copy수를 측정하여 그 복제 조절 기능에 초래된 변화를 확인하였다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2000년도 춘계학술대회논문집
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• pp.167-170
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• 2000

Recently the sub-micron structured substrates of 0.74 ${mu}ell$ track pitch and 800 $\AA$groove depth are required for DVD-RAM and the track pitch is expected to be narrower as the need for the information storage density is getting higher. For the accurate replication of the land-groove structure in the stamper to the plastic substrates it is important to control the injection -compression molding process such that the integrity of the replication for the land-groove structure is maximized. in the present study polycarbonate substrates were fabricated by injection comression molding and the land-groove structure regarded as one of mold temperature and the compression pressure on the integrity of the replication were examined experimentally. An efficient design methodology using the response surface method (RSM) the central composite design(CCD) technique and the analysis-of-variance (ANOVA) was developed to obtain the optimum processing conditions which maximize the integrity of the replication with a limited number of experiments.

• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.837-842
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• 2013

A cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733 was isolated, characterized, and used for the construction of a cloning vector to engineer Leuconostoc species. pMBLR00 is a rolling circle replication plasmid, containing 3,370 base pairs. Sequence analysis revealed that pMBLR00 has 3 open reading frames: Cop (copy number control protein), Rep (replication protein), and Mob (mobilization protein). pMBLR00 replicates by rolling circle replication, which was confirmed by the presence of a conserved double-stranded origin and single-stranded DNA intermediates. An Escherichia coli-Leuconostoc shuttle vector, pMBLR02, was constructed and was able to replicate in Leuconostoc citreum 95. pMBLR02 could be a useful genetic tool for metabolic engineering and the genetic study of Leuconostoc species.