[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.1304.04009

Isolation and Characterization of a Cryptic Plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733

Chae, Han Seung (Department of Molecular Science and Technology, Ajou University)
Lee, Jeong Min (Department of Medical Science, Ajou University)
Lee, Ju-Hoon (Department of Food Science, Kyung Hee University)
Lee, Pyung Cheon (Department of Molecular Science and Technology, Ajou University)

Publication Information

Journal of Microbiology and Biotechnology / v.23, no.6, 2013 , pp. 837-842 More about this Journal

Abstract

A cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733 was isolated, characterized, and used for the construction of a cloning vector to engineer Leuconostoc species. pMBLR00 is a rolling circle replication plasmid, containing 3,370 base pairs. Sequence analysis revealed that pMBLR00 has 3 open reading frames: Cop (copy number control protein), Rep (replication protein), and Mob (mobilization protein). pMBLR00 replicates by rolling circle replication, which was confirmed by the presence of a conserved double-stranded origin and single-stranded DNA intermediates. An Escherichia coli-Leuconostoc shuttle vector, pMBLR02, was constructed and was able to replicate in Leuconostoc citreum 95. pMBLR02 could be a useful genetic tool for metabolic engineering and the genetic study of Leuconostoc species.

Keywords

Cryptic plasmid; Leuconostoc mesenteroides; rolling circle replication mechanism; ${\small{D}}$ -lactic acid;

Citations & Related Records

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