• Title/Summary/Keyword: Rep. of Korea

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Inhibition of Angiogenesis by Propolis

  • Song, Yun-Seon;Park, Eun-Hee;Jung, Kyung-Ja;Jin, Changbae
    • Archives of Pharmacal Research
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    • v.25 no.4
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    • pp.500-504
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    • 2002
  • Propolis, obtained from honeybee hives, has been used in Oriental folk medicine as an anti-inflammatory, anti-carcinogenic, and immunomodulatory agent. There is considerable evidence suggesting that angiogenesis and chronic inflammation are codependent. Blockage of angiogenesis results in an anti-inflammatory effect. Ethanol (EEP) and ether extracts of propolis (REP), and caffeic acid phenethyl ester (CAPE), an active component of propolis, were examined for their anti-angiogenic activities using the chick embryo chorioallantoic membrane (CAM), and the calf pulmonary arterial endothelial (CPAE) cell proliferation, assays. The presence of EEP, REP and CAPE inhibited angiogenesis in the CAM assay and the proliferation of CPAE cells. The results suggest that anti-angiogenic activities of EEP, REP and CAPE are also responsible for their anti-inflammatory effect.

A Localized Software-based Approach for Fault-Tolerant Ethernet (LSFTE)

  • Vu, Huy Thao;Kim, Se Mog;Pham, Anh Hoang;Rhee, Jong Myung
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.3 no.3
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    • pp.51-61
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    • 2010
  • Nowadays, there are various networked systems with many computers. In most networked systems, a crucial objective is to keep transmitting and/or receiving data continuously even though failures exist. How can one make a computer continue transmitting and/or receiving data even when there are some errors on a link? Fault-Tolerant Ethernet (FTE) can be a solution to this question. In this paper, we propose a Localized Software-based Fault-Tolerant Ethernet (LSFTE). Our new approach fulfills the general FTE requirements. It takes advantage of redundant cable lines to maintain communication in a faulty environment. A software layer, which uses a simple and effective algorithm, is added above the LAN card driver software to detect and overcome faults. For our approach, there is no need to change the existing hardware or the end-use interfaces. Furthermore, the fault-detection time is reduced significantly compared to the conventional software-based approach.

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Molecular Typing of Staphylococcus aureus Strains from Domestic Animals and Humans by REP-PCR Analysis (REP-PCR을 이용한 국내 사람과 동물유래 Staphylococcus aureus 분리주의 Molecular Typing)

  • Woo Yong-Ku;Kim Shin
    • Korean Journal of Microbiology
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    • v.41 no.1
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    • pp.60-66
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    • 2005
  • To select the rapid and efficient molecular subtyping method for epidemiologic monitoring of Staphylococcus aureus (S. aureus) strains at clinical laboratory levels, a total 116 of S. aureus and MRSA (methicillin-resistant S. aureus) strains from diverse animal species [Korean cattle, goat, pig, dog, chicken, mouse] and also humans were analyzed. To evaluate the discriminatory ability (DA) of individual PCR methods, random amplified polymorphic of DNA [RAPD; 4M & RA primer], repetitive extragenic palindromic sequences PCR (REP-PCR), and enterobacterial repetitive intergenic consensus sequences PCR (ERIC-PCR) methods were conducted and then compared on their Simpson's index of diversity (SID) values based on the dendrogram patterns, which was produced by software program (BiolD2+ & GelCompar II). In first, RAPD using the 4M primer (SID 0.915) was expressed more higher SID value than that of RA primer (SID 0.874). 4M primer was expressed more powerful DA than RA. Both REP-PCR (SID 0.930) and ERIC-PCR (SID 0.929) methods showed much more higher DA than that of RAPD. According to the present results, both REP-PCR and ERIC-PCR among the tested analysis methods were found as the most reliable and discriminative molecular subtyping method, because they expressed the highest DA for the present S. aureus and MRSA strains.

Replication origin (ori) of R-plasmid pSBK203 Isolated from Staphylococcus aureus DHI (Staphylococcus aureus DH1에서 분리한 R-plasmid pSBK203상의 복제개시 부위 ori에 관한 연구)

  • Min, Kyung-Il;Byeon, Woo-Hyeon
    • Korean Journal of Microbiology
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    • v.32 no.3
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    • pp.186-191
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    • 1994
  • The origin of the leading strand replication (ori) and of lagging strand replication (M-O) of R-plasmid pSBK203 was identified and its base sequence was determined. About 50 bp of ori sequence residues overlapped with the structural gene of rep. Sequence comparison reveals that pSBK-ori shares obvious identities with those of pT181 family and consists of two regions, one is conserved and the other is variable region. Of two palindrome sequence located one after another in upstream region of rep gene, palA' instead of palA which shares sequence homology with diverse family of plasmids such as pOX6, pC194, and pE194 seems to act as a signal for conversion of primarily replicated ssDNA to dsDNA (minus origin (M-O)).

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Characterization of the Replication Region of the Enterococcus faecalis Plasmid p703/5

  • Song, Joon-Seok;Park, Jin-Hwan;Kim, Chan-Wha;Kim, Young-Woo;Lim, Wang-Jin;Kim, Ick-Young;Chang, Hyo-Ihl
    • Journal of Microbiology and Biotechnology
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    • v.9 no.1
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    • pp.91-97
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    • 1999
  • In this work, a 1.9-kb region of enterococcal plasmid p703/5 was isolated and the nucleotide sequence analysis of the region was performed. One major open reading frame (ORF) was identified encoding a polypeptide of 28 kDa. Database comparisons suggested that the protein showed some homology with other bacterial RepA proteins. Upstream of the ORF, a potential dnaA box, AT-rich region and 22-bp tandemly repeated sequences (DNA iterons), a feature typical for many replication ori sites, were recognized. Deletion analysis using Exonuclease III and several restriction enzymes indicated that the three elements and the gene product from the ORF were essential for replication and that the minimum unit of DNA required for replication resided on the 1.2-kb AvaII subfragment. Thus, this gene product was referred to as RepA.

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Oropharyngeal Carriage and Antimicrobial Resistance of S. pneumoniae in Children of Seoul (서울 지역 소아의 구인강에서 폐구균 보균율과 항균제 내성)

  • Kim, Young Kee;Lee, Chang Kyu
    • Pediatric Infection and Vaccine
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    • v.4 no.2
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    • pp.218-224
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    • 1997
  • Purpose: The antimicrobial resistance of S. pneumoniae has encountered with increasing frequency from around the world. In our country, penicillin resistant strains of S. penumococci are rapidly increasing. It has been known that colonized pneumococci in upper respiratory tract cause sinisitis, otitis media, meningitis and pneumonia. We tried to reveal the colonization rate of pneumonocci in upper respiratory tract, their antimicrobial resistance and DNA fingerprinting pattern in normal children. Methods: We got specimens from 117 children of day-care center in Seoul through oropharyngeal swab. After incubation on BAP, optochin test and slide latex agglutination test were used for identification. Antimicobial susceptibility test to penicillin, vancomycin, erythromycin and TMP-SMZ was done with disk diffusion method. Penicillin MIC was gotten through the broth microdilution method. Genotyping of 45 pneumococci was done by rep-PCR using REP1R-Dt and REP2-Dt primer. Results: The carriage rate of pneumococci in the day-care center children was 38%(45/117). The resistance of penicillin, erhthromycin, TMP/SMZ, vancomycin by the disk diffusion method are 89%, 91%, 64% and 0%, respectively. 64% of the isolates showed multiple resistance. 7 types of DNA fingerprinting were gotten and 78% of isolates belonged to three types. Conclusion: We found that the antimicrobial resistance of children attending the day-care center in Seoul was much higher than expected. We assumed that this might be due to their easy and frequent exposure to antimicrobial agents and crowded day-care center environment.

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Changes of Rural Landscape in the lifted Green-belt Area Using Resident Employed Photography(REP) (거주민 참여 사진촬영 방법(REP)를 활용한 개발제한구역 해제에 따른 근교 농촌 경관변화 분석)

  • Yun, Seung-Yong;Son, Yong-Hoon
    • Journal of Korean Society of Rural Planning
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    • v.24 no.4
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    • pp.15-25
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    • 2018
  • This study was designed to understand the change of rural landscape and to consider problems followed by development restrictions lifted for Neobiul Village in Ansan City, Korea. Physical landscape changes were comprehended by a field study and interview with local residents, and the residents' perception regarding the landscape changes were analyzed with the REP investigation method. The results can be summarized into the following three points: First, due to the lift of development restrictions and the deregulation of land use, the number of factories and warehouses for rent increased, which became a new source of income for the village. Second, the residents' complaints increased due to the increased traffic volume and waste from a sudden influx of factories and warehouses, which could not be handled by a small farming village. Third, a mix of landscape combining both city and farming village was formed due to the influx of external capital and the need of rental income, although the residents rather wanted Neobiul Village to become a residential village than a factory location. Furthermore, even in the farmlands near the village where development restrictions have not been lifted, the level of dependence on the farming industry has decreased as a consequence of the increase in farmland rent and weekend farms. This paper confirmed that the change of rural landscape followed by lifted development restrictions affects the everyday life of residents living in Neobiul Village. This study has significant implications in that it suggests a case showing the effects of national policies such as lifting development restrictions for rural villages in suburban areas.

Validation of Efficient Topological Data Model for 3D Spatial Queries (3차원 공간질의를 위한 효율적인 위상학적 데이터 모델의 검증)

  • Lee, Seok-Ho;Lee, Ji-Yeong
    • Spatial Information Research
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    • v.19 no.1
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    • pp.93-105
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    • 2011
  • In recent years, large and complex three-dimensional building has been constructed by the development of building technology and advanced IT skills, and people have lived there and spent a considerable time so far. Accordingly. in this sophisticatcd three-dimensional space, emergencies services or convenient information services have been in demand. In order to provide these services efficiently, understanding of topological relationships among the complex space should be supported naturally. Not on1y each method of understanding the topological relationships but also its efficiency can be different depending on different topological data models. B-rep based data model is the most widely used for storaging and representing of topological relationships. And from early 2000s, many researches on a network based topological data model have been conducted. The purpose of this study is to verify the efficiency of performance on spatial queries. As a result, Network-based topological data model is more efficient than B-rep based data model for determining the spatial relationship.