• 한국식생활문화학회지
• /
• 제17권4호
• /
• pp.456-464
• /
• 2002

3% 갈근 열수 추출액 급여와 카드뮴을 흰쥐에게 4주 동안 급여 한 후 간과 신장 조직의 카드뮴 함량, renin 호르몬 농도, 혈청중의 GPT, GOT 및 LDHase의 함량을 조사하였다. 식이섭취량에서 대조군인 24.62 g에 비하여, 3% 갈근 열수 추출액 급여군은 23.41 g으로, 카드뮴 급여군은 23.76 g에 비하여, 갈근 열수 추출액급여군과 카드뮴 병합 급여군은 22.25 g으로 감소하였으나 유의한 차이는 없었다. 체중 증가량은 대조군이 124.50 g이고, 3% 갈근 열수 추출액급여군은 127.22 g으로 대조군에 비하여 감소하였다. 카드뮴 급여군은 107.57 g에 비하여, 갈근 열수 추출액급여군과 음용수와 카드뮴 병합 급여군은 128.80 g으로 유의적으로 증가하였다. 식이효율은 카드뮴 급여군과 갈근- 카드뮴 병합 급여군 간에는 유의성이 인정되었다. 간의 무게에서 대조근에 비하여 갈근 열수 추출액 급여군이 약간 감소하였으나 유의한 차이는 없었으며 카드뮴 공급군에 비하여 갈근-카드뮴 병합 급여군이 유의적으로 증가하였다. 신장 무게는 카드뮴 공급군에 비하여 갈근 카드뮴 병합 급여군이 유의적으로 증가하였다. 간 조직내에서의 카드뮴 함량은 대조군이 0.15 ug/g으로 갈근 열수 추출액급여군은 0.14 ug/g에 비하여 별다른 차이를 보이지 않았다. 카드뮴 급여군은 3.04 ug/g에 비하여 갈근-카드뮴 음용수 병합 급여군은 2.87 ug/g 유의적으로 감소하였다. 신장 조직내에서 대조군은 0.21 ug/g이었으나, 갈근 열수 추출액급여군 은 0.23 ug/g으로 별다른 차이는 없었다. 카드뮴 급여군은 6.48 ug/g에 비하여 갈근 열수 추출액급여군과 카드뮴 병합 급여군은 4.42 ug/g 4.57ug/g으로 유의적으로 감소하였다. 혈장호르몬인 renin 농도는 갈근차 음용수군은 16.73 ngAl/mL/hr으로 대조군인 15.89 ngAl/mL/hr에 비해서 증가하였다. 카드뮴 급여군은 25.72 ngAl/mL/hr으로 카드뮴과 갈근 열수 추출액 병합 급여군인 18.57ngAl/mL/hr에 비하여 유의성 있게 증가하였다. GOT는 대조군이 96.16 U/L에 비하여 갈근차 단독 급여군은 96.44 U/L로 약간 증가하였다. 카드뮴 급여군은 154.40 U/L인데 비하여 카드뮴과 갈근 열수 추출액 병합 급여군이 118.80 U/L으로 유의적으로 감소하였다. GPT는 대조군이 59.60 U/L에 비하여, 갈근 열수 추출액 급여군은 63.46 U/L으로 약간 증가하였으나 유의한 차이는 없었다. 카드뮴 급여군은 73.54 U/L 인데 비하여 갈근 열수 추출액 급여군과 카드뮴 병합 급여군은 69.80 U/L으로 유의적으로 감소하였다. LDHase는 대조군이 179.00, 갈근 열수 추출액급여군은 198.60 U/L으로 대조군에 비하여 감소하였으나 유의한 차이는 나지 않았다. 카드뮴 급여군은 264.30 U/L 인데 비하여 갈근 열수 추출액급여군와 카드뮴 동시 병합 급여군은 227.30 U/L으로 유의적으로 감소하였다. 카드뮴에 중독된 흰쥐에 대한 갈근 추출물의 해독 효과를 알아 보는 본 연구에서 50 ppm의 카드뮴액과 함께 3% 갈근 추출액을 급여한 흰쥐의 체중증가량과 사료 섭취 효율을 50 ppm의 카드뮴만을 급여한 흰쥐의 이들 측정값과 비교할 때 유의적인 차이가 없었다. 그러나 50 ppm의 카드뮴액과 함께 3% 갈근 추출액을 급여한 흰쥐군은 50 ppm의 카드뮴액만을 급여한 흰쥐군과 비교하여 신장내 카드뮴 함량과 GPT 및 LDH 활성도, renin 활성도가 유의적으로 감소되었고 신장 무게는 정상 흰쥐와 같은 수준으로 회복하였고 GOT 활성도 역시 정상 흰쥐와 같은 수준으로 감소를 보여 갈근이 카드뮴 중독 흰쥐에서 신장 등의 장기내 카드뮴 축적의 감소로 카드뮴 중독 작용의 경감 효과를 갖는 것으로 나타났다.

• Annals of Clinical Neurophysiology
• /
• 제3권1호
• /
• pp.50-52
• /
• 2001

Licorice is widely used as a Chinese(herbal) medicine. The glycyrrhizin, a main ingredient of the natural licorice, has a potent mineralocorticoid effect which may cause severe hypokalemia and muscle paralysis. We present a 60-year-old woman, who had been ingesting one or two spoonful of licorice powder daily for about one year, developed acute flaccid quadriparesis with high levels of serum muscle enzymes and the typical features of mineralocorticoid excess such as severe hypokalemia and metabolic alkalosis. Both plasma renin activity and serum aldosterone level were below the normal values. This case indicates that licorice-induced hypokalemic myopathy should be considered in the differential diagnosis of a patient with acute quadriparesis and hypokalemia.

• 한국환경독성학회:학술대회논문집
• /
• 한국환경독성학회 2002년도 추계국제학술대회
• /
• pp.165-165
• /
• 2002

Hypertension is considered to be caused by a complicated combination of genetic and environmental factors. Atrial natriuretic peptide (ANP) has been to suppress renin activity and inhibit the synthesis and release of aldosterone. Therefore, Abnormalities of this peptide caused by genetic variation may be influence the blood pressure. The aim of present study was to examine the relationship between hypertension and Sca I RFLP of ANP gene in Korean population. The genotype distribution of this RFLP was significantly different between normotensives and hypertensives (P<0.05). However, this genetic marker was not significantly associated with any anthropometric parameters or plasma lipid concentrations in our study group. Therefore, our result suggest that Sca I RFLP of ANP gene may be useful as genetic marker in the ethiology of hypertension in Korean population, independent of any cardiovascular risk. factors studied.

• 동의생리병리학회지
• /
• 제16권3호
• /
• pp.490-494
• /
• 2002

The purpose of this study is to investigate the effect of Shudihuang water extracts on the cGMP production of medulla and cortical membranes, receptors for atrial natriuretic peptide in the kidney by in vitro autoradiography in rats for 8, 16 weeks. The cGMP production of medullary and cortical membranes of the kidney decreased after the administration of Shudihuang water extracts. The density of receptors for atrial natriuretic peptide in the kidney decreased after the administration of Shudihuang water extracts only for 16 weeks. These results suggest that the long term administration of Shudihuang water extracts has decreased plasma renin activity and plasma levels of aldosterone modulated cGMP production of medullary and cortical membranes, density of receptors for atrial natriuretic peptide in the kidney.

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2001년도 추계 수산관련학회 공동학술대회발표요지집
• /
• pp.183-184
• /
• 2001

Angiotensin I converting enzyme (ACE) in renin-angiotensin system is a cause of essential hypertension, which covers most hypertension, one of the major adult diseases. Thus, the inhibition of ACE would be indispensable for the prevention and cure of hypertension. Therefore, a lot of studies on the ACE inhibitor have been conducted. Peptides from the protein hydrolysate have been reported as an remarkable inhibitor. Especially, various ACE inhibitory peptides were isolated and identified from marine products for their utilization as value added products. (omitted)

• Journal of Chest Surgery
• /
• 제25권2호
• /
• pp.200-204
• /
• 1992

A 63 year old male had suffered from hypertension and angina pectoris for 4 years, On physical examination, blood pressure was 150/110 mmHg with medication of antihypertensive drugs. Aortogram showed the stenosis of the left renal artery, the complete occlusion of the right renal artery, and atherosclerotic change of abdominal aorta. Blood urea nitrogen was 25 mg/dl, serum creatinine was 1.2 mg/dl, and renin activity in peripheral blood was 8.7 ng /ml /hour, The stenosis of left renal artery and the complete occlusion of right renal artery should have produced the renovascular hypertension Bilateral aorto-renal bypasses with saphenous grafts were done for treatment of ren-ovascular hypertension Postoperatively, blood pressure was normalized with only small dosage of antihypertensive drugs.

• BMB Reports
• /
• 제31권5호
• /
• pp.459-467
• /
• 1998

A new set of functional group interaction energy descriptors relevant to the ACE (Angiotensin-Converting Enzyme) inhibitory peptide, QSAR (Quantitative Structure Activity Relationships), is presented. The functional group interaction energies approximate the charged interactions and distances between functional groups in molecules. The effective energies of the computationally derived geometries are useful parameters for deriving 3D-QSAR models, especially in the absence of experimentally known active site conformation. ACE is a regulatory zinc protease in the renin-angiotensin system. Therapeutic inhibition of this enzyme has proven to be a very effective treatment for the management of hypertension. The non bond interaction energy values among functional groups of six-feature of ACE inhibitory peptides were used as descriptor terms and analyzed for multivariate correlation with ACE inhibition activity. The functional group interaction energy descriptors used in the regression analysis were obtained by a series of inhibitor structures derived from molecular mechanics and semi-empirical calculations. The descriptors calculated using electrostatic and steric fields from the precisely defined functional group were sufficient to explain the biological activity of inhibitor. Application of the descriptors to the inhibition of ACE indicates that the derived QSAR has good predicting ability and provides insight into the mechanism of enzyme inhibition. The method, functional group interaction energy analysis, is expected to be applicable to predict enzyme inhibitory activity of the rationally designed inhibitors.

• 대한한방내과학회지
• /
• 제19권2호
• /
• pp.159-184
• /
• 1998

The aim of the study was the experiment of the effect that Kami-Daesihotang had on the essential hypertension and hyperlipidemia. Rats were orally administered with Kami-Daesihotang for 30days and the constituent of the plasma and serum were analysed at the 10th, 20th and 30th day from the first day of experiment, respectively. The heart rate, blood pressure, plasma renin activity, plasma level of aldosterone, catecholamine, sodium and angiotensin II were measured after an oral administration of Kami-Daesihotang in SHR. In addition, serum levels of total cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol and total lipid were measured with cholesterol-fed rats. The results were summarized as following ; 1. Single-dosage Kami-Daesihotang & double-dosage Kami-Daesihotang remarkably decreased the blood pressure in SHR. 2. Double-dosage Kami-Daesihotang were recognized as having the effect on the decreased of the pulse rate in SHR. 3. Plasma renin activity was significantly decreasd in SHR after single-dosage Kami-Daesihotang & double-dosage Kami-Daesihotang treatment. 4. Double-dosage Kami-Daesihotang considerably reduced the plasma angiotensin level in SHR. 5. Noticeable decreased of plasma norepinephrine level was showed in SHR, after single-dosage Kami-Daesihotang & double-dosage Kami-Daesihotang treatment. 6. Single-dosage Kami-Daesihotang & double-dosage Kami-Daesihotang noticeable reduced body weight in hyperlipidemia rats which had fed with 1% cholesterol. 7. Single-dosage Kami-Daesihotang & double-dosage Kami-Daesihotang had a significantly decreasing effect on serum total cholesterol in hyperlipidemia rats which had fed with 1% cholesterol. 8. Serum triglyceride level was importantly decreased in hyperlipidemia rats which had fed with 1% cholesterol, after single-dosage Kami-Daesihotang & double-dosage Kami-Daesihotang treatment. 9. Remarkable decreased of serum low density lipoprotein cholesterol level was found in hyperlipidemia rats which had fed with 1% cholesterol, after single-dosage Kami-Daesihotang & double-dosage Kami-Daesihotang treatment. 10. Double-dosage Kami-Daesihotang was showed a significantly decreasing effect on serum total lipid level in hyperlipidemia rats which had fed with 1% cholesterol. 11. Single-dosage Kami-Daesihotang noticeably reduced organ weight of liver, kidney, spleen and testis in hyperlipidemia rats which had fed with 1% cholesterol. Double-dosage Kami-Daesihotang significantly decreased organ weight of liver, kidney and spleen in hyperlipidemia rats. These Findings suggest a possible anti-hypertensive and hyperlipidemic effect of Kami-Daesihotang.

• The Korean Journal of Physiology and Pharmacology
• /
• 제1권3호
• /
• pp.325-335
• /
• 1997

Evidence for the existance of at least two subclasses of renal adenosine receptors has been presented. N-6-cyclohexyladenosine (CHA) is a relatively selective $A_1$ adenosine agonists, whereas 5'-N-ethylcarboxamidoadenosine (NECA) acts as a preferential agonist of $A_2$ adenoisne receptor. N6-(L-2-phenylisoproryl)-adenosine (PIA) almost unselectively activates both $A_1\;and\;A_2$ adenosine receptors at micromolar concentrations. During the characterization of adenosine receptor in the kidney, we have discovered a novel phenomenon, that is, an intramuscular administration of CHA for 3 days caused a diuresis and a suppression of urinary concentrating ability. To further characterize this novel phenomenon, an intramuscular administration of adenosine and other adenosine angonists, PIA and NECA, and prior treatment of adenosine antagonists, caffeine, theophylline and 1,3-diethyl-8-phenyl-xanthine (DPX) were performed. Systemic administration of CHA, PIA, and NECA for 3 days caused a suppression in heart rate, blood pressure and general motor activity without change in rectal temperature. Systemic administration of CHA, 0.5, 1 and 2 mg/kg/day, for 3 days caused a dose-dependent increase in urine volume and decrease in urinary osmolarity and free water reabsorption. This phenomenon was reversible and repeatable. Administration of adenosine (40 mg/kg/day) produced no apparent effect on the renal function, whereas PIA (2 mg/kg/day) produced an similar effect to CHA on the renal function. Systemic adminstration of NECA, 0.025, 0.05 and 0.25 mg/kg/day, for 3 days caused a dose-dependent increase in urine volume and dose-dependent increases in excreted amount of creatinine, urinary osmolarity and free water reabsorption. These renal effects of adenosine agonist were maximum at second day during the drug administration. In terms of increase in urine volume and the suppression of urinary concentrating ability, NECA was potent than CHA. Prior treatment of caffeine (50 mg/kg/day) or theophylline (50 mg/kg/day) abolished the diuretic effect of CHA, whereas DPX (50 mg/kg/day) did not affect the CHA effect. CHA, 0.5 mg/kg/day, produced no change in plasma renin activity and plasma levels of aldosterone, epinephrine, and norepinephrine. These results suggest that this novel phenomenon produced by an activation of renal adenosine receptors plays an important role in urinary concentrating mechanism.

• 대한핵의학회지
• /
• 제19권2호
• /
• pp.69-79
• /
• 1985

To evaluate the effect of dopaminergic activity on aldosterone secretion, the plasma renin activity, serum cortisol and aldosterone were measured by radioimmunoassay in 6 normal controls and 12 patients who had hyponatremia and generalized edema or ascites with possible condition with secondary aldosteronism before and after(15, 30, and 60 min) 15 mg of metoclopramide by iv bolus injection and same method with 500 mg of L-dopa by per oral in 6 normal controls and 12 patients with edema ascites. The result were as follows; 1) The basal level of PRA was higher in patients rather than normal controls but PRA was not influenced by MC or L-dopa administration on both normal controls and patients group. 2) The serum cortisol level was significantly elevated at 30 min after MC injection compared with basal level in normal controls but no significant change was noted in patients group. After L-dopa administration the serum cortisol level was not changed in both normal controls and patients group. 3) The serum aldosterone level was significantly elevated in 15, 30 and 60 min after MC injection in normal controls, and there also same tendency of aldosterone secretion was noticed in patients group. On the other hands, there was no changes in aldosterone level in both normal controls and patients group with L-dopa administration. Above result means that MC stimulate aldosterone secretion by dopaminergic antagonist and aldosterone secretion in normal subject is controlled by maximal tonic dopaminergic inhibition. In edematous patients, however, both of the dopaminergic inhibitory and stimulating effect of PRA, ACTH etc on the aldosterone secretion seems to be variable.