• 대한수의학회지
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• 제34권4호
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• pp.653-664
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• 1994

This study was carried out to investigate diabetic renal changes in cyclophosphamide(CY) treated nonobese diabetic(NOD) mice and to develop animal model of diabetic human nephropathy. The 8-week-old NOD and ICR mice were injected with cyclophosphamide intraperitoneally at 200mg/kg body weight and compared the chemical effects on these mice with the non-treated NOD and ICR mice respectively. The renal glomeruli in ICR, cyclophosphamide-treated ICR(ICR+CY), NOD and cyclophosphamide-treated NOD(NOD+CY) mice were observed by the light and electron microscopes. The results obtained were summarized as follows ; 1. Spontaneous incidences of diabetes mellitus in NOD mice were significantly promoted by dosing with cyclophosphamide. 2. Glomerulohypertrophy, proliferation of mesangium, partial thickening of glomerular basement membrane, and partial fusion of pedicels of podocyte were observed in NOD mice and NOD+CY mice. These changes were not observed in both ICR mice and ICR+CY mice. 3. The diabetic nephropathy observed in NOD+CY mice was more severe than that of non-treated NOD mice.

• 생명과학회지
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• 제8권4호
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• pp.449-456
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• 1998

태생 13일된 토끼의 발생기 신장에서 뇨세관과 사구체 세포들의 크기와 형태를 관찰하여 성숙한 토끼의 신장과 형태계측학적으로 비교한 결과 다음과 같은 결과를 얻었다. 1. 태생 13일의 발생기 뇨세관 단면적은 성숙한 토끼의 근위세관 단면적보다 훨씬 넓고 평균직격이 길었으며 내강이 거의 없었다. 뇨세관 상피세포의 솔모양 가장자리는 발달되지 않았고 핵 위쪽에 둥근 사립체가 발달되었으며 기저막부분에는 사립체가 적었다. 2. 발생기 사구체내에는 벽측세포, 족세포, 내피세포들로 구별되었으며 성숙한 토끼보다 내피세포와 벽측세포의 세포핵 단멱적이 훨씬 컸고 성숙한 토끼의 사구체에서는 족세포가 가장 컸으나 발생기에는 내피세포의 크기가 가장 컸다.

• Childhood Kidney Diseases
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• 제7권2호
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• pp.223-228
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• 2003

Oligomeganephronia는 선천적인 신형성 부전으로 신원의 수가 감소되고 보상성으로 사구체가 비대해진 질환으로 지금까지 전 세계적으로 약 60례만이 보고될 정도로 매우 드문 질환이다. 저자들은 학교선별뇨검사상 현미경적 혈뇨를 보였던 환아에서 신생검을 통하여 oligomeganephronia를 조기에 진단하고, 4년간 신부전증 및 성장부전의 진행 소견과 함께 사구체 경화증의 발생으로 사구체 용적이 감소하는 소견을 관찰할 수 있었기에, 이를 문헌 고찰과 함께 보고하는 바이다.

• 융합신호처리학회논문지
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• 제13권2호
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• pp.70-76
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• 2012

본 연구에서는 신장 조직 영상으로부터 자동적으로 사구체 영역을 추출하는 방법을 제안한다. 사구체 조직은 신장의 상태를 나타내는 많은 정보를 포함하고 있기 때문에 사구체 영역의 추출은 신장검사를 자동화하기위한 첫 번째 단계이다. 신장 조직 영상은 신장조직조각을 현미경을 통해서 CCD 카메라로 촬영함으로써 얻어진다. 특히, 사구체 영역과 타 영역과의 차이는 명확치 않아 기존 분할방법에 의해 그 배경으로부터 사구체 영역을 추출하는 일은 쉽지않다. 이에 여러 가지 모양을 갖는 사구체 영역의 경계 에지를 공통의 특징으로서 주목하였다. 우선 가우스 함수에 의한 원영상의 몽롱화 영상을 동적인 임계값으로서 사용하여 이 임계값에 의해 원영상을 2치화 한다. 다음으로 획득한 영상으로부터 일반적인 패턴처리 기법으로 사구체 영역의 경계 에지를 포함하는 모든 에지를 추출한다. 그 다음으로 사구체 영역은 폐곡선에 의해 둘러쌓인 원영상내의 영역을 추출함으로써 얻어진다. 그 결과 사구체 영역이 85%정도 정확하게 추출되어 제안 방법의 유효성을 확인하였다.

• 대한본초학회지
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• 제33권4호
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• pp.1-7
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• 2018

Objectives : Diabetic nephropathy is one of the most significant chronic complications of diabetes. Advanced glycation end products (AGEs) have been implicated in the development of diabetic nephropathy. GS-E3D is an enzymatic modified red ginseng extract by pectin lyase and has an increased concentration of the ginsenoside Rd compared to an unmodified red ginseng extract. In this study, we evaluated the preventive effects of GS-E3D on renal dysfunction in the type 2 diabetic db/db mice. Methods : GS-E3D (100 or 250 mg/kg body weight per day) was given to db/db mice through oral gavage for 6 weeks. Body weight and blood glucose levels were examined. At the end of the experiment, albuminuria was measured. The renal tissues were collected for histological examination, and immunohistochemical staining was used to detect renal accumulation of AGEs and podocyte loss Results : In the db/db mice, severe hyperglycemia developed, and albuminuria was significantly increased. Diabetes induced markedly morphological alterations to the renal glomerular cells. AGE accumulations and podocyte loss were detected in renal glomeruli. No difference in blood glucose levels was noted between GS-E3D-treated and vehicletreated diabetic db/db mice. However, GS-E3D treatment significantly reduced albuminuria and AGE accumulations in diabetic mice. Moreover, the loss of podocytes was restored by GS-E3D treatment. Conclusions : GS-E3D might be beneficial for the treatment of diabetic nephropathy. The ability of GS-E3D on to attenuate albuminuria and podocyte dysfunction in the db/db mice may be mediated by the inhibition of AGE accumulation.

• Applied Microscopy
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• 제21권2호
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• pp.76-95
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• 1991

A single injection of cadmium chloride (3.75 mg/kg) was made into the peritoneal cavities of albino rats. The cortices of kidney were obtained from the experimental animals at 3 hr., 6 hr., 12 hr., 24 hr. and 36 hr. after administration of cadmium chloride, respectively. The specimens of each experimental animal were prefixed in 2% glutaraldehyde-4% paraformaldehyde solution for $2{\sim}4$ hours, and these specimens were post-fixed in 1% osmic acid. After fixation, the specimens were dehydrated with alcohol and acetate and embedded in Epon 812. Ultrathin sections, $600{\sim}800{\AA}$ thickness were made and stained with uranyl acetate and lead citrate. And all the preparations were observed with Hitachi-600 transmission electron microscope. The results obtained were as follows: 1. The main changes in ultrastructures of the glomeruli observed at 3 hr. after cadmium chloride administration include loss of filtration slit and fenestrae of capillary endothelium that was resulted from thickings of the basal lamina and fusion of pedicels of the podocytes. At 12 hr. after cadmium chloride administration the Bowman's capsules were mostly filled with abnormally thickened and fused pedicels. After 24 hr. however, the only recognized change was loss of fenestrae of the capillary endothelium. And the ultrastructure of the glomeruli were almost normal in 36 hr. after cadmium chloride treatment. 2. At 3 hr. after treatment with cadmium chloride, in the renal tubular cells the vesicles and vacuoles increased in number at the apical portion, of the tubular epithelial cells, the basal infoldings were reduced and the basal lamina was thickened. After 12 hr., a number of phagosomes appeared at the apical portion and the cisternae of rough endoplasmic reticulum were swollen. At 24 hr. after cadmium chloride administration irregularly shaped mitochondria were observed in the apical area, and mitochondria with swollen cristae were found at the basal portion. And after 36 hr. The ultrastructures of the epithelial cells appeared almost normal except for a moderate increase in the number of vesicles and vacuoles. Consequently it is suggested that in albino rats, cadmium chloride induces acute reversible degenerative changes in the glomeruli as well as in the epithelial cells of the proximal convoluted tubules.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제26권6호
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• pp.652-657
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• 2000

The kidneys play a vital role in the maintenance of normal body fluid volumes and in the composition of the extracellular fluid compartments. There are normally more than 2 million functioning glomeruli that regulate total body water and solute concentrations. As renal failure progresses, there is a decrease in the number of functioning nephrons. Chronic renal failure(CRF) is the consequence of a multitude of diseases that cause permanent destruction of the nephron. Azotemia is an elevation in blood urea nitrogen(BUN) and serum creatinine levels subsequent to a decreased glomerular filtration rate(GFR), which results in uremia. This loss of renal function can cause functional and metabolic abnomalities of body. For this problem, oral & maxillofacial surgeons have demanded to routinely treat patients with CRF. However, there has not been a reported case of orthognathic surgery by bilateral sagittal split ramus osteotomy(BSSRO) in patients with CRF, which can cause multiple complications in healthy patients. We report developmental mechanism of complication associated with CRF and preop. and postop. care of orthognathic surgery by BSSRO in Cl III patient with severe chronic renal failure.

• 대한수의학회지
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• 제39권6호
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• pp.1126-1140
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• 1999

Renal failure is one of the main causes of economic impacts in the poultry industry and complex syndrome with different severity of clinical signs caused by multiple nephropathogenic factors such as infectious bronchitis viral infection and excess salt and calcium in diet. To evaluate the correlation between severity of renal failure and the causative nephropathogenic factors, one-day-old specific pathogen free chicks were treated with either single causative factor or multiple causative factors described as above. Each group was designed as control for non-treated control, IB for infectious bronchitis virus (IB virus) infection, IBHNa for IB virus infection with high diet salt, IBHCa for IB virus infection with high diet calcium, IBHNC for IB virus infection with high diet salt and calcium, HNa for high diet salt, HCa for high diet calcium and HNC for high diet salt and calcium. Chickens were inoculated with IB virus at 1-day-old and remained on their respective diets until 21 day of age. The high dietary salt feeding groups such as IBHNa, IBHNC, HNa, HNC increased water intake, watery diarrhea, general subcutaneous edema and the high dietary calcium feeding groups such as IBHCa and IBHNC showed severe visceral gout. Two more than treated groups caused high mortality in comparison with the single treated groups. IB virus exposure significantly increased urate deposition and lymphocytic interstitial nephritis. Especially urate deposition dramatically increased when excess diet calcium was combined together. In excess diet salt treated groups enlarged edematous kidneys were observed and hypertrophy of glomeruli were showed. These results suggest that IB virus enhanced the incidence and severity on chicken renal failure clearly related to the quantity of salt and calcium.

• Childhood Kidney Diseases
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• 제10권1호
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• pp.83-88
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• 2006

기립성 단백뇨는 안정시에는 요단백이 검출되지 않고 환동시에만 검출되는 양성질환으로, 정확한 병인은 확립되어 있지 않으나 최근 그 원인으로써 nutcracker 현상을 보고한 바 있다. 대부분 방사선학적 검사를 통하여 진단하고 추적관찰 동안에 저절로 단백뇨의 소실을 보이나, 본 증례의 경우, 24시간 소변의 단백이 1.5 g/일 이상 검출되고 3년 이상 지속되어 신조직의 병변을 알고자 신생검을 실시하여 메산지움 증식성 병리소견을 보였기에 문헌고찰과 함께 보고하는 바이다.

• 한국환경보건학회지
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• 제22권4호
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• pp.91-101
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• 1996

Exposure indices are important tools which enable scientists to reliably predict and detect exposures to xenobiotics and resultant cell injury. Since the de novo synthesis of stress proteins can be detected early after exposure to some agents, analysis of toxicant-induced changes in gene expression, i.e. alterations in patterns of protein synthesis, may be useful to develop as biomarkers of exposure and toxicity. The acute and chronic effects of cadmium(Cd, $CdCl_2$ 20 mg/kg) on Wistar male rats were evaluated concerning cadmium contents, tissues enzyme activity, HSP expression. The results of the study were as follows: 1. Less cadmium was absorbed through the digestive tracts, but the ratio of contents in renal to hepatic cadmium was higher at 8 weeks after treatment. 2. ALT(alanine aminotransferase), AST(aspartate aminotransferase), glucose, BUN(blood urea nitrogen), creatinine, the key indices of the clinical changes in hepatic and renal function were significantly changed by the cadmium treatment after 1 week in liver, after 4 weeks in kidney. 3. Enhanced synthesis of 70 KDa relative molecular mass proteins were detected in 2 hours after cadmium exposure, with maximum activity occurring at 8~48 hours. Induction of $HSP_{70}$ was evident at proximal tubules and glomeruli in kidney. Testicular cells produced enough HSP to be detected normally. From the above results, it could be concluded that $HSP_{70}$ induction by the cadmium treatment was a rapid reaction to indicate the exposure of xenobiotics.