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http://dx.doi.org/10.6116/kjh.2018.33.4.1.

Preventive Effects of Pectin Lyase-Modified Red Ginseng Extract on renal injury in db/db mice  

Kim, Chan-Sik (Korea Institute of Oriental Medicine)
Jo, Kyuhyung (Korea Institute of Oriental Medicine)
Pyo, Mi Kyung (International Ginseng & Herb Research Institute)
Kim, Jin Sook (Korea Institute of Oriental Medicine)
Kim, Junghyun (Korea Institute of Oriental Medicine)
Publication Information
The Korea Journal of Herbology / v.33, no.4, 2018 , pp. 1-7 More about this Journal
Abstract
Objectives : Diabetic nephropathy is one of the most significant chronic complications of diabetes. Advanced glycation end products (AGEs) have been implicated in the development of diabetic nephropathy. GS-E3D is an enzymatic modified red ginseng extract by pectin lyase and has an increased concentration of the ginsenoside Rd compared to an unmodified red ginseng extract. In this study, we evaluated the preventive effects of GS-E3D on renal dysfunction in the type 2 diabetic db/db mice. Methods : GS-E3D (100 or 250 mg/kg body weight per day) was given to db/db mice through oral gavage for 6 weeks. Body weight and blood glucose levels were examined. At the end of the experiment, albuminuria was measured. The renal tissues were collected for histological examination, and immunohistochemical staining was used to detect renal accumulation of AGEs and podocyte loss Results : In the db/db mice, severe hyperglycemia developed, and albuminuria was significantly increased. Diabetes induced markedly morphological alterations to the renal glomerular cells. AGE accumulations and podocyte loss were detected in renal glomeruli. No difference in blood glucose levels was noted between GS-E3D-treated and vehicletreated diabetic db/db mice. However, GS-E3D treatment significantly reduced albuminuria and AGE accumulations in diabetic mice. Moreover, the loss of podocytes was restored by GS-E3D treatment. Conclusions : GS-E3D might be beneficial for the treatment of diabetic nephropathy. The ability of GS-E3D on to attenuate albuminuria and podocyte dysfunction in the db/db mice may be mediated by the inhibition of AGE accumulation.
Keywords
Diabetic nephropathy; GS-E3D; Red ginseng;
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