• The Korean Journal of Physiology and Pharmacology
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• 제5권5호
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• pp.397-405
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• 2001

The ryanodine receptor, a $Ca^{2+}$ release channel of the sarcoplasmic reticulum (SR), is responsible for the rapid release of $Ca^{2+}$ that activates cardiac muscle contraction. In the excitation-contraction coupling cascade, activation of SR $Ca^{2+}$ release channel is initiated by the activity of sarcolemmal $Ca^{2+}$ channels, the dihydropyridine receptors. Previous study showed that the relaxation defect of diabetic heart was due to the changes of the expressional levels of SR $Ca^{2+}$ATPase and phospholamban. In the diabetic heart contractile abnormalities were also observed, and one of the mechanisms for these changes could include alterations in the expression and/or activity levels of various $Ca^{2+}$ regulatory proteins involving cardiac contraction. In the present study, underlying mechanisms for the functional derangement of the diabetic cardiomyopathy were investigated with respect to ryanodine receptor, and dihydropyridine receptor at the transcriptional and translational levels. Quantitative changes of ryanodine receptors and the dihydropyridine receptors, and the functional consequences of those changes in diabetic heart were investigated. The levels of protein and mRNA of the ryanodine receptor in diabetic rats were comparable to these of the control. However, the binding capacity of ryanodine was significantly decreased in diabetic rat hearts. Furthermore, the reduction in the binding capacity of ryanodine receptor was completely restored by insulin. This result suggests that there were no transcriptional and translational changes but functional changes, such as conformational changes of the $Ca^{2+}$ release channel, which might be regulated by insulin. The protein level of the dihydropyridine receptor and the binding capacity of nitrendipine in the sarcolemmal membranes of diabetic rats were not different as compared to these of the control. In conclusion, in diabetic hearts, $Ca^{2+}$ release processes are impaired, which are likely to lead to functional derangement of contraction of heart. This dysregulation of intracellular $Ca^{2+}$ concentration could explain for clinical findings of diabetic cardiomyopathy and provide the scientific basis for more effective treatments of diabetic patients. In view of these results, insulin may be involved in the control of intracellular $Ca^{2+}$ in the cardiomyocyte via unknown mechanism, which needs further study.

• 대한지역사회영양학회지
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• 제8권5호
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• pp.781-793
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• 2003

This study was performed to investigate the stress level and health-related behaviors of nurses and to provide basic information for developing educational programs in the health care field. The subjects of this study were 197 nurses and 94 nursing assistants working at hospitals in the Kyung-nam area. A survey was conducted using a self-administered questionnaire in November, 2002. The results were as follows : The average age and work experience of the subjects were 26.9 and 5.6 years, respectively. The number of respondents in the shift and non-shift operations was equally distributed. The mean height and weight were 161.2 cm and 52.7 kg, respectively. Even though the average body mass index (BMI) and obesity index were normal in the subjects, 9.2% of the subjects were overweight/obese, while 28.9% of the subjects were under weight, according to the obesity index. The mean score of stress was 27.5 \pm$\pm$ 4.6 out of 50 points. Most of the subjects were highly stressed about the amount of their work, problems regarding their future, and relationships with their superiors. With regard to changes in food intake due to stress, 44.1% showed an increased intake, while 32.3% showed a reduced intake. The degree of health consciousness of the married nurses, those over 36 years of age, and those with over 10 years of nursing experience was significantly higher than that of the unmarried nurses, those under 35 years of age, and those with under 10 years of nursing experience (p<0.01). Fifty-one point three percent of the subjects thought their health status was unhealthy. Fifty-seven percent of the subjects had tried weight control. Their main reason for trying weight control was to have a slender figure (62.3%), and their methods of weight control were moderation in intake(45.6%), exercise and dieting (36.9%). TV/radio (49.5%) and newspaper/magazines (47.1%) were the primary sources of nutritional and health information for the subjects, and professional (25.1%) and the internet (13.4%) were ranked relatively highly. The food components of most concern to the subjects during meal time were (in order of importance) fats (51.9%), calories (40.2%) and salt (35.1%). The subjects considered 'taking a rest' (73.5%) as the most important factor in maintaining an optimal health status, followed by self-relaxation (56.4%), moderation in diet (39.5), bathing or using a sauna (25.7%) and exercising (22.7%).

• The Korean Journal of Physiology and Pharmacology
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• 제4권1호
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• pp.33-40
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• 2000

This study was designed to clarify the mechanism of the inhibitory action of a nitric oxide (NO) donor, 3-morpholino-sydnonimine (SIN-1), on contraction, cytosolic $Ca^{2+}$ level $([Ca^{2+}]_i)$ and ionic currents in guinea-pig ileum. SIN-1 $(0.01{\sim}100\;{\mu}M)$ inhibited 25 mM KCl- or histamine $(10\;{\mu}M)-induced$ contraction in a concentration-dependent manner. SIN-1 reduced both the 25 mM KCl- and the histamine-stimulated increases in muscle tension in parallel with decreased $[Ca^{2+}]_i.$ Using the patch clamp technique with a holding potential of -60 mV, SIN-1 $(10\;{\mu}M)$ decreased peak Ba currents $(I_{Ba})$ by $30.9{\pm}5.4%$ (n=6) when voltage was stepped from -60 mV to +10 mV and this effect was blocked by ODQ $(1\;{\mu}M),$ a soluble guanylyl cyclase inhibitor. Cu/Zn SOD (100 U/ml), the free radical scavenger, had little effect on basal $I_{Ba},$ and SIN-1 $(10\;{\mu}M)$ inhibited peak $I_{Ba}$ by $32.4{\pm}5.8%$ (n=5) in the presence of Cu/Zn SOD. In a cell clamped at a holding-potential of -40 mV, application of $10\;{\mu}M$ histamine induced an inward current. The histamine-induced inward current was markedly and reversibly inhibited by $10\;{\mu}M$ SIN-1, and this effect was abolished by ODQ $(1\;{\mu}M).$ In addition, SIN-1 markedly increased the depolarization-activated outward $K^+$ currents in the all potential ranges. We concluded that SIN-1 inhibits smooth muscle contraction mainly by decreasing $[Ca^{2+}]_i$ resulted from the inhibition of L-type $Ca^{2+}$ channels and the inhibition of nonselective cation currents and/or by the activation of $K^+$ currents via a cGMP-dependent pathway.

• Korean Journal of Radiology
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• 제20권5호
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• pp.830-843
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• 2019

Objective: To examine the potential of intravoxel incoherent motion (IVIM) and blood oxygen level-dependent (BOLD) magnetic resonance imaging for detecting renal changes after iodinated contrast-induced acute kidney injury (CI-AKI) development in a diabetic rabbit model. Materials and Methods: Sixty-two rabbits were randomized into 2 groups: diabetic rabbits with the contrast agent (DCA) and healthy rabbits with the contrast agent (NCA). In each group, 6 rabbits underwent IVIM and BOLD imaging at 1 hour, 1 day, 2 days, 3 days, and 4 days after an iohexol injection while 5 rabbits were selected to undergo blood and histological examinations at these specific time points. Iohexol was administrated at a dose of 2.5 g I/kg of body weight. Further, the apparent transverse relaxation rate (R2^*), average pure molecular diffusion coefficient (D), pseudo-diffusion coefficient (D^*), perfusion fraction (f) were calculated. Results: The D and f values of the renal cortex (CO) and outer medulla (OM) were significantly decreased compared to baseline values in the 2 groups 1 day after the iohexol injection (p < 0.05). A marked reduction in the D^* values for both the CO and OM was also observed after 1 hour in each group (p < 0.05). In the OM, a persistent elevation of the R2^* was detected for 4 days in the DCA group (p < 0.05). Histopathological changes were prominent, and the pathological features of CI-AKI aggravated in the DCA group until day 4. The D, f, and R2^* values significantly correlated with the histological damage scores, hypoxia-inducible transcription factor-1α expression scores, and serum creatinine levels. Conclusion: A combination of IVIM and BOLD imaging may serve as a noninvasive method for detecting and monitoring CI-AKI in the early stages in the diabetic kidney.

• 경영정보학연구
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• 제22권2호
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• pp.137-166
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• 2020

대한민국 국민의 해외여행 비율은 매년 증가 추세인데 반해, 국내여행 비율은 수년간 답보 상태에 있다. 이에, 정부에서는 다양한 국내 관광 활성화 정책을 시행하고 있으나, 이를 통한 국내관광 증진 효과는 제한적이다. 국내관광을 활성화시키기 위해서는, 여행자들별로 선호하는 여행 방식에 차이가 있음을 이해하고, 이에 맞춤화된 여행 서비스를 제공할 필요가 있다. 본 연구는 대한민국 국민들의 세대별 여행 방식의 특징을 분석한 후, 각 세대별로 맞춤화된 여행 서비스를 구성하기 위한 시사점을 도출하였다. '세대'란 같은 시대에 살면서, 비슷한 생애주기에 유사한 내/외부의 경험을 하기 때문에(김기연 등, 2003), 다른 세대와는 구별되는, 그 세대만의 관광 방식이 있을 수 있다. 본 연구는 여행상품 구성의 관점에서 세대 간 차이를 살펴보았다. 즉, 여행 상품 구성 및 마케팅에 필요한 요소인, 여행정보 수집 방식 및 출처, 사전 예약 상품의 종류, 패키지 이용 여부, 여행 시기/기간 및 장소, 여행시 주요활동 그리고, 여행만족도에 영향을 미치는 요인 등에 대한 세대 간 차이를 파악하고 시사점을 도출하였다. 본 연구에는 한국 문화체육관광연구원에서 수집한 2017년 국민 여행 실태조사 데이터 16,713건이 활용되었으며, 데이터는 패널들의 세대에 따라서, 밀레니얼(19세~34세), X세대(35세~54세), 베이비부머(55세~64세), 시니어(65세 이상) 등으로 구분하여 사용하였다. 본 연구결과, 전 세대 모두 자연경관이 수려하고, 문화유산이 풍부하며, 숙박 시설이 쾌적할 때, 여행만족도가 유의미하게 향상되었다. 또한, 전체 패키지보다는 숙박, 차량 대여 등 개별 상품을 쉽게 구매할 수 있도록 하고, 맛집 탐방 상품을 제공하는 것도 유효할 수 있을 것이다. 각 세대별로는, 밀레니얼은 여름철 성수기에 인기 방문지를 중심으로 체험 상품을 구성하는 것이 좋으며, 이들을 위한 관광 안내 시설을 잘 마련할 필요가 있겠다. 또한, 포털사이트와 소셜네트워크 서비스를 통한 마케팅도 밀레니얼 세대에게 효과적일 것으로 보인다. X세대는 자가용으로 여행하기 좋은 지역에, 자녀와 함께 할 수 있는 체험형 여행상품이 유효할 것으로 보인다. 이들에게는 교통만족도가 중요하며, 여름철 성수기에 인기 관광지로 여행을 많이 간다는 특징은 밀레니얼 세대와 유사하다. 베이비부머와 시니어 세대의 경우, 자연 감상 및 휴식, 쇼핑 등을 포함한 여행 상품을 구성하는 것이 좋을 것으로 보이며, 인터넷을 통한 마케팅보다는 구전 효과를 이용하는 것이 유효하겠다. 특히 시니어 세대의 경우, 봄가을을 중심으로, 당일 여행 패키지 상품을 구성하는 것도 효과적일 것으로 보인다. 이러한 세대별 여행 특성을 고려하여 맞춤화된 관광상품을 구성한다면, 궁극적으로 국내관광산업을 활성화에 기여할 수 있을 것으로 기대된다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권9호
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• pp.1205-1215
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• 2012

This study was conducted to establish genetic criteria for phenotypic characteristics of Hanwoo cattle based on allele frequencies and genetic variance analysis using microsatellite markers. Analysis of the genetic diversity among 399 Hanwoo cattle classified according to nose pigmentation and coat color was carried out using 22 microsatellite markers. The results revealed that the INRA035 locus was associated with the highest $F_{is}$ (0.536). Given that the $F_{is}$ value for the Hanwoo INRA035 population ranged from 0.533 (white) to 1.000 (white spotted), this finding was consistent with the loci being fixed in Hanwoo cattle. Expected heterozygosities of the Hanwoo groups classified by coat colors and degree of nose pigmentation ranged from $0.689{\pm}0.023$ (Holstein) to $0.743{\pm}0.021$ (nose pigmentation level of d). Normal Hanwoo and animals with a mixed white coat showed the closest relationship because the lowest $D_A$ value was observed between these groups. However, a pair-wise differentiation test of $F_{st}$ showed no significant difference among the Hanwoo groups classified by coat color and degree of nose pigmentation (p<0.01). Moreover, results of the neighbor-joining tree based on a $D_A$ genetic distance matrix within 399 Hanwoo individuals and principal component analyses confirmed that different groups of cattle with mixed coat color and nose pigmentation formed other specific groups representing Hanwoo genetic and phenotypic characteristics. The results of this study support a relaxation of policies regulating bull selection or animal registration in an effort to minimize financial loss, and could provide basic information that can be used for establishing criteria to classify Hanwoo phenotypes.

• 한국컴퓨터정보학회논문지
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• 제25권10호
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• pp.211-219
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• 2020

본 연구는 일정기간 여성들에게 체형관리를 실시하여 생활습관, 사회적 지지, 생활만족도의 잠재변수 간 구조적 관계와 삶의 질에 미치는 영향을 파악하는 것이다. 체형관리는 카이로프랙틱 교정법과 근막이완요법으로 시술이 진행되었고, 25년 경력을 소지한 물리치료사를 전문가로 활용하였다. 연구대상자는 광주광역시에 거주하는 20대에서 60대 사이의 여성을 표본으로 하였고, 연구를 위해 체형관리 시술을 20회 이상 참여한 여성에게 설문지를 자가 평가 기입법으로 작성하게 하여 그 중 설문 응답이 정확하고 자료 분석하기에 타당하게 이루어진 100명을 최종 분석 대상으로 하였다. 연구결과 여성의 건강관련 삶의 질은 체형관리 유무와 정도에 따라 삶의 질이 달라질 수 있다는 것을 알 수 있었다. 또한 생활습관과 사회적 지지도의 정도에 따라 삶의 만족도는 달라지고 삶의 질과 관련된 잠재변수들 간의 직접효과, 간접효과, 총 효과 모두 유의미한 것으로 나타났다. 본 연구에서는 여성의 삶의 질을 향상시킬 수 있는 평소의 체형관리가 여성 스스로 바람직한 생활습관을 갖도록 하는 계기와 삶의 질을 중요시하는 인식을 갖게 해주는 것에 의미를 부여한다.

• The Korean Journal of Physiology
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• 제28권2호
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• pp.169-179
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• 1994

The effect of extracellular and intracellular pH on vascular tone and $^{45}Ca$ uptake were investigated in aortic strips and dispersed single aortic smooth muscle cells of spontaneously hypertensive rats (SHR) and aged-matched Wistar-Kyoto rats (WKY). The contraction produced by a change of extracellular pH (pHo) in the range of $6.5{\sim}8.3$ was estimated by comparison with the level of vascular tone at pH 7.4. Contraction was induced below pHo 6.5 in WKY, pHo 7.1 in SHR, and over pHo 8.0 on both strains. The amplitude of contraction induced by high pHo (over pHo 7.7) was similar in SHR and WKY, but that induced by low pHo (below pHo 7.1) in SHR was greater than that in WKY. Either high pHo- or low pHo-induced contractions in WKY and SHR were not induced in the Ca-free Tyrode's solution and were induced by the addition of Ca. $^{45}Ca$ uptake increased progressively as pHo was increased from 6.8 to 8.1 in the single aortic smooth muscle cells of WKY and SHR. $NH_4Cl$ induced a gradually developing contraction in a dose-dependent manner $(5\;mM{\sim}30\;mM)$ and the removal of $NH_4Cl$ induced transient contraction was followed by profound relaxation in the aortic rings of both strains. The contractions induced by $NH_4Cl$ or by the removal of $NH_4Cl$ in SHR were significantly greater than that in WKY. These contractions were not induced in Ca-free Tyrode's solution. $^{45}Ca$ uptake was increased by $NH_4Cl$ (20 mM) and was not changed by the removal of $NH_4Cl$ (20 mM) in the aortic strips of WKY and SHR. As a summary of above results, the vascular tone of SHR was more sensitive to the change pHi and pHo than that of WKY. The contractions induced by change of extracellular or intracellular pH depended on extracellular Ca in the aorta of SHR nnd WKY. However, the Ca uptake was in accord with the changes of contraction but increase in contraction by low pH was not accompanied by an increase in Ca uptake in both strains.

• 한국산학기술학회논문지
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• 제11권12호
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• pp.5165-5176
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• 2010

본 연구는 최근 극심한 쇠퇴현상을 겪고 있는 천안역 앞 명동패션거리 일대를 대상으로 도심재생차원에서의 가로활성화 방안을 모색한다. 천안시내 기존도심 지역의 물리 환경적 측면, 역사 문화적 측면, 사회 경제적 측면에서 현황과 문제점들을 검토하고, 아울러 가로 방문자들을 대상으로 가로이용행태와 가로활성화와 관련한 의식을 조사하였다. 연구를 통해 제안된 해당 지역의 잠정적인 가로활성화 방안들을 요약하면 다음과 같다. 물리 환경적 측면에서는 기존도심 토지소유자들의 개발동기를 유발할 수 있도록 법적 인센티브 장치를 마련하는 것이 요구되며, '명동거리' 내에 휴식 및 녹지공간 조성, 기존건물을 활용한 문화예술 공간의 확충과 함께 지하공간을 활용한 공영주차장 조성 등이 제안된다. 사회 경제적 측면에서는 기존 가로별 업종 특성을 최대한 살리면서 획일적이지 않도록 '명동거리', '은행길', '옛시청길' 등 가로별로 독특한 테마를 설정하고 각각의 테마업종들이 예시된다. 역사 문화적 차원에서는 기존 도심이 갖고 있는 역사적 요소들을 적극적으로 발굴하여 역사탐방로 등의 프로그램을 개발하고 기존 패션거리와 연계하여 보행네트워크화 하는 방안이 제시된다.

• 대한임상전기생리학회지
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• 제5권2호
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• pp.61-72
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• 2007

INTRODUCTION: Local heat and cold application has been frequently used as means of muscle relaxation and blood circulation or reinforcing muscle strength, relaxing muscle tension in clinical situation. In particular, it has been known that long-term heat and cold application for relaxing muscle tension inhibits muscle spasticity or tension. But, it has been rarely reported that what influences of heat and cold application on activation of muscle action potential. Therefore, this study aims to analyze surface temperature and electromyography activities according to the heat and cold application. METHODE: Subjects of this research were 10 normal men and women (5 men, 5 women). Hot pack and cold pack was applied to vastus medialis muscle of thigh and rectus femoris muscle for 20 min. Surface temperature of vastus medialis muscle and rectus femoris muscle was measured, knee joint of subjects was in $45^{\circ}$ flexion, sitting on a chair, maximal isometric contraction was induced, surface electromyography (sEMG) signals were collected and root mean square (RMS) and median frequency (MOF) were analyzed. All measurements were conducted before and immediately after experiment, 10 min., 20 min. and 30 min. after experiment. Data were analyzed with SPSS 12.0 program, comparison of changes in superficial temperature and sEMG signals through repeated measurement was conducted with repeated measures ANOVA and significance level $\alpha$ was 0.05. RESULTS: Changes of surface temperature of vastus medialis muscle according to cold application were radically decreased immediately after application, but it was recovered after 30 min. of application and it showed significant difference (F4. 36=72.216, P<0.001). Surface temperature of rectus femoris also showed radical decrease immediately after application, but it was recovered after 30 min. of application and showed significant difference (F4. 36=88.930, P<0.001). Changes of surface temperature of vastus medialis muscle according to heat application were radically increased immediately after application, but it was recovered after 30 min. of application and it showed significant difference (F4. 36=27.267, P<0.001). Surface temperature of rectus femoris also showed radical decrease immediately after application, but it was recovered after 30 min. of application and showed significant difference (F4. 36=19.774, P<0.001). Changes of sEMG by heat and cold application were no statistical difference. Surface temperature of skeletal muscle after heat and cold application showed significant change for 30 min., but it was found that increase or decrease of surface temperature had not great influence on sEMG activities.