• 제목/요약/키워드: Relative growth ratio

검색결과 381건 처리시간 0.024초

Direct Analysis of the Transcription of Escherichia coli rnpB Gene Harbored in a Multicopy Plasmid during Bacterial Growth

  • Park, Jeong-Won;Jung, Young-Hwan;Park, Bo-Hyun;Jeoung, Yeon-Hee;Lee, Young-Hoon
    • BMB Reports
    • /
    • 제29권3호
    • /
    • pp.221-224
    • /
    • 1996
  • To examine the growth-phase dependent control of Escherichia coli rnpB gene we used a combination of Northern analysis for RNA determination and Southern analysis for plasmid DNA determination. The relative amounts of metabolically unstable transcript derived from the internally deleted rnpB gene harbored on a multicopy plasmid as well as the relative plasmid contents were measured by Northern analysis and Southern analysis, respectively, of total nucleic acids from E coli cells containing the plasmid. The relative transcription activity of the rnB was represented by a ratio of the relative amount of the transcript to that of the plasmid DNA during bacterial growth. The rnpB transcription increased rapidly with time during exponential growth, but started to decrease before the transition period of an exponential growing cell culture into the stationary phase. Although the expression pattern was similar to the changes of ${\beta}-galactosidase$ activity expressed from the lysogenic strain carrying the chromosomal rnpB-lacZ fusion which were shown in a previous work, the present data appears to represent a more actual growth-phase control of the rnpB transcription than the previous data by the ${\beta}-galactosidase$ assay. In addition the present method described for a direct analysis of both RNA and plasmid DNA provides a rapid and efficient method that can applied to an examination of transcription control by using a multicopy plasmid.

  • PDF

Effect of Extrusion Processing and Steam Pelleting Diets on Pellet Durability, Water Absorption and Physical Response of Macrobrachium rosenbergii

  • Misra, Chandra Kanta;Sahu, N.P.;Jain, K.K.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제15권9호
    • /
    • pp.1354-1358
    • /
    • 2002
  • Two hundred and ten post-larvae (PL) of Macrobrachium rosenbergii (14.5-14.9 mg) were equally distributed in two experimental groups and fed with either steam cooked or extruded pellet for a period of 60 days. Physical evaluation and growth promoting effect of both the pellets were assessed. Significantly higher (p<0.05) water stability, absorption and protein efficiency ratio (PER) were recorded in extruded pellet than the steam cooked pellet. Nutrient loss was minimum in the extruded pellet for which lower feed conversion ratio (FCR) (p<0.05) was recorded in this group. However, weight gain, relative growth and specific growth rate (SGR) in both the groups were not significantly different (p>0.05). Although insignificant (p>0.05) but higher amylase activity was recorded in steam pellet fed group. Survival was not affected by feeding either of the diets.

광도가 곰솔 유묘의 생장과 물질생산에 미치는 영향 (Effects of Light Intensity on Growth and Biomass Production of Pinus thunbergii Deedings)

  • 김종진
    • 임산에너지
    • /
    • 제19권1호
    • /
    • pp.7-12
    • /
    • 2000
  • 본 실험은 광도가 곰솔(Pinus thunbergii Parl.) 유묘의 생장과 물질생산에 어떠한 영향을 미치는 가를 알고자 자연전광이 100, 50, 30, 10% 및 2%로 조절된 야외포지에서 1997년 5월부터 1998년 10월까지 실시되었다. 곰솔의 수고생장 및 근원경생장은 1,2차년을 통하여 자연전광에서 가장 높악으며, 50%에서의 수고생장은 1,2차년도 각각 대조구의 96.4, 88.9%로, 근원경생장은 각각 94.1 77.6%로 조상되엇다, 30%이하로 상대광도가 낮아질수록 수고 및 근원경생장이 급격히 감소 되었으며, 근원경생장의 감소율이 수고생장의 감소율보다 높게 나타났다. 2차년도의 2%에서는 시험 유묘 모두가 고사하였다. 물질생산량도 광도가 낮아짐에 따라 감소되었으며, 1, 2차년도 모두 뿌리의 감소율이 잎, 줄기보다 높았고, 1차년도에는 줄기의 감소율이, 2차년도에는 잎이 감소율이 가장 낮았다. T/R율은 1차년도에는 50%에서,3.55, 2차년도에는 10%에서 4.88로 가장 높았다.

  • PDF

Growth Performance, Relative Meat and Organ Weights, Cecal Microflora, and Blood Characteristics in Broiler Chickens Fed Diets Containing Different Nutrient Density with or without Essential Oils

  • Kim, Sang-Jin;Lee, Kyung-Woo;Kang, Chang-Won;An, Byoung-Ki
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제29권4호
    • /
    • pp.549-554
    • /
    • 2016
  • The present study was conducted to investigate whether dietary essential oils could affect growth performance, relative organ weights, cecal microflora, immune responses and blood profiles of broiler chickens fed on diets containing different nutrient densities. A total of eight hundred-forty 1-d-old male broiler chicks were randomly allotted into twenty-eight pens (7 pens per treatment, 30 chicks per pen). There were four experimental diets containing two different nutrient densities and supplemented with or without essential oils. Experimental period lasted for 35 days. No clear interaction between nutrient density and essential oils on any of growth performance-related parameters was observed. Live body weights were affected (p<0.05) by nutrient density at 21 days and by dietary essential oils at 35 days. Essential oils significantly (p<0.05) increased daily body weight gain and feed conversion ratio during the periods of 22 to 35 and 1 to 35 days, but failed to affect feed intake during the entire experimental period. Daily weight gain at 1 to 21 days and feed intake at 1 to 21 and 1 to 35 days were significantly impaired (p<0.05) by nutrient density. There were significant treatment interactions (p<0.05) on relative weights of bursa of Fabricius and abdominal fat contents. Finally, either essential oil or nutrient density did not influence the relative percentages of breast and leg meats, the population of cecal microflora, blood parameters and antibody titers against Newcastle disease and infectious bronchitis in broiler chickens. It was concluded that dietary essential oils, independent to nutrient density, failed to stimulate feed intake, but increased growth performance in broiler chickens.

Effect of High Dietary Carbohydrate on the Growth Performance, Blood Chemistry, Hepatic Enzyme Activities and Growth Hormone Gene Expression of Wuchang Bream (Megalobrama amblycephala) at Two Temperatures

  • Zhou, Chuanpeng;Ge, Xianping;Liu, Bo;Xie, Jun;Chen, Ruli;Ren, Mingchun
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제28권2호
    • /
    • pp.207-214
    • /
    • 2015
  • The effects of high carbohydrate diet on growth, serum physiological response, and hepatic heat shock protein 70 expression in Wuchang bream were determined at $25^{\circ}C$ and $30^{\circ}C$. At each temperature, the fish fed the control diet (31% CHO) had significantly higher weight gain, specific growth rate, protein efficiency ratio and hepatic glucose-6-phosphatase activities, lower feed conversion ratio and hepatosomatic index (HSI), whole crude lipid, serum glucose, hepatic glucokinase (GK) activity than those fed the high-carbohydrate diet (47% CHO) (p<0.05). The fish reared at $25^{\circ}C$ had significantly higher whole body crude protein and ash, serum cholesterol and triglyceride, hepatic G-6-Pase activity, lower glycogen content and relative levels of hepatic growth hormone (GH) gene expression than those reared at $30^{\circ}C$ (p<0.05). Significant interaction between temperature and diet was found for HSI, condition factor, hepatic GK activity and the relative levels of hepatic GH gene expression (p<0.05).

The effect of Acacia nilotica bark extract on growth performance, carcass characteristics, immune response, and intestinal morphology in broilers as an alternative to antibiotic growth promoter

  • Muhammad Umer Zahid;Anjum Khalique;Shafqat Nawaz Qaisrani;Muhammad Ashraf;Ali Ahmad Sheikh;Muhammad Umar Yaqoob
    • Animal Bioscience
    • /
    • 제36권7호
    • /
    • pp.1059-1066
    • /
    • 2023
  • Objective: Present study was designed to evaluate the efficacy of Acacia nilotica bark extract as an alternative to antibiotic growth promoters in broilers. Methods: Six hundred, day-old broiler chicks were randomly divided into six groups (NC, without any supplementation; AB, NC+Zinc Bacitracin; PB, NC+Safmannan; ANBE1, NC+A. nilotica bark extract 0.1%; ANBE3, NC+A. nilotica bark extract 0.3%; ANBE5, NC+A. nilotica bark extract 0.5%), with ten replicates per group (10 chicks/replicate) and feeding trial was lasted for 35 days. Results: Results showed that weight gain (1,296.63 g) and feed conversion ratio (FCR, 1.59) of AB was better than NC, during the finisher phase. Overall FCR of AB (1.53), PB (1.54), and ANBE5 (1.54) was significantly (p<0.05) better than NC. From carcass parameters relative weight of wing and heart were highest in ANBE3 (2.5% and 1.51%, respectively). Significantly (p<0.05) highest blood glucose level was observed in NC (264.5 mg/dL) and highest albumin concentration was found in AB (1.46 mg/dL). In addition, antibody titer levels against ND and IBD were higher in ANBE5 than NC, while higher relative weight of bursa was observed in ANBE3 than NC. The villus height to crypt depth ratio in all experimental groups was better than NC. Conclusion: Acacia nilotica bark extract could be a suitable alternative to antibiotic growth promoters to support the growth in broilers.

열탄화법을 사용한 탄화규소 나노와이어의 성장 (Growth of SiC Nanowire Using Carbothermal Reduction Method)

  • 노대호;김재수;변동진;양재웅;김나리
    • 한국재료학회지
    • /
    • 제13권10호
    • /
    • pp.677-682
    • /
    • 2003
  • SiC nanowires were synthesized by carbothermal reduction using metal catalysts. Synthesized nanowires had mean diameters of 30∼50 nm and several $\mu\textrm{m}$ length. The kind of catalysts affects form of SiC nanowire because of difference of growth mechanisms. These differences were made by catalyst's physical property and relative activities to the source gas. Ni acted a conventional catalyst of VLS growth mechanism. But, Case of Fe, SiC nanowire was grown by stable VLS growth mechanism without relation of growth conditions. SiC nanowire was grown by two step growth model using Cr catalyst. Conversion ratios to the SiC nanowire were increased with growth conditions. Case of Cr, conversion ratio was about 45% that was higher than other catalyst used. This high conversion ratio was obtained by the addition VS growth to radial direction on the as-grown nanowires.

Influence of elastic T-stress on the growth direction of two parallel cracks

  • Li, X.F.;Tang, B.Q.;Peng, X.L.;Huang, Y.
    • Structural Engineering and Mechanics
    • /
    • 제34권3호
    • /
    • pp.377-390
    • /
    • 2010
  • This paper studies fracture initiation direction of two parallel non-coplanar cracks of equal length. Using the dislocation pile-up modelling, singular integral equations for two parallel cracks subjected to mixed-mode loading are derived and the crack-tip field including singular and non-singular terms is obtained. The kinking angle is determined by using the maximum hoop stress criterion, or the ${\sigma}_{\theta}$-criterion. Results are presented for simple uniaxial tension and biaxial loading. The biaxiality ratio has a noticeable influence on crack growth direction. For the case of biaxial tension, when neglecting the T-stress the crack branching angle is overestimated for small crack inclination angles relative to the largest applied principal stress direction, and underestimated for large crack inclination angles.

토양산성화가 봉선화(Impatiens balsamina L.) 및 만수국(Tagetes patula L.)의 생장에 미치는 영향 (Effects of Soil Acidification on Growth of Impatiens balsamina L. and Tagetes patula L. Plants)

  • 김학윤
    • 생명과학회지
    • /
    • 제11권2호
    • /
    • pp.153-158
    • /
    • 2001
  • To investigate the effects of soil acidification on growth of Impatiens balsamina L. plants were transplanted to acidified soils with H$_2$SO$_4$ solution. The concentrations of soluble Ca, Mg, K, Al and Mn in the acidified soils increased with increment of H$^{+}$ addition to the soil. In both species, the plant height and root length were inhibited by soil acidification, showing much severer inhibition in Impatiens balsamina L. than in Tagetes patula L., As the soil pH decreases, the growth of underground parts decreased greatly than that of above ground parts in both species. Total dry weight decreased with increased Al concentration as well as lowered soil pH in both plants. There was a strong positive correlation between relative total dry weight and molar (Ca+Mg+K) / Al ratio of the soil. The results suggest that molar(Ca+Mg+K)/ Al ratio of the soil may be useful indicator for assessing the critical load of acid deposition in herb species.s.

  • PDF

전기장을 받는 강유전체 내의 전도균열 성장거동 (Conducting Crack Growth Behavior in Ferroelectrics Subjected to Electric Fields)

  • 정경문;박재연;범현규
    • 한국정밀공학회:학술대회논문집
    • /
    • 한국정밀공학회 2002년도 추계학술대회 논문집
    • /
    • pp.820-823
    • /
    • 2002
  • The asymptotic problem of a semi-infinite conducting crack parallel to the poling direction in ferroelectric ceramics subjected to electric fields is analyzed. The main mechanism for the conducting crack growth behavior is thought to be ferroelectric domain switching leading to the development of a process zone around the crack. The shape and size of the switching zone is shown to depend strongly on the relative magnitude on the ratio of the coercive electric field to the yield electric field. It is shown that the crack growth can be either enhanced or retarded depending on the ratio of the coercive electric field to yield electric field.

  • PDF