• Journal of Microbiology and Biotechnology
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• 제23권12호
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• pp.1747-1756
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• 2013

The aim of this paper was to study the effect of spent medium recycle on Spodoptera exigua Se301 cell line proliferation, metabolism, and baculovirus production when grown in batch suspension cultures in Ex-Cell 420 serum-free medium. The results showed that the recycle of 20% of spent medium from a culture in mid-exponential growth phase improved growth relative to a control culture grown in fresh medium. Although both glucose and glutamine were still present at the end of the growth phase, glutamate was always completely exhausted. The pattern of the specific glucose and lactate consumption and production rates, as well as the specific glutamine and glutamate consumption rates, suggests a metabolic shift at spent medium recycle values of over 60%, with a decrease in the efficiency of glucose utilization and an increase in glutamate consumption to fuel energy metabolism. Baculovirus infection provoked a change in the metabolic pattern of Se301 cells, although a beneficial effect of spent medium recycle was also observed. Both growth rate and maximum viable cell density decreased relative to uninfected cultures. The efficiency of glucose utilization was dramatically reduced in those cultures containing the lowest percentages of spent medium, whereas glutamine and glutamate consumption was modulated, thereby suggesting that infected cells were devoted to virus replication, retaining their ability to incorporate the nutrients required to support viral replication. Recycle of 20% of spent medium increased baculovirus production by around 90%, thus showing the link between cell growth and baculovirus production.

• Journal of Ecology and Environment
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• 제29권1호
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• pp.29-34
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• 2006

Three macrophytes species, that are, Phragmites australis, Zizania latifolia and Typha angustifolia were grown in mono culture in order to compare growth (relative biomass increase rate), variation of photosynthetic pigment (total chlorophyll, Chl a, Chl b and Chl a/Chl b) and effectiveness of nutriment removal in 15%o NaCl-salt solution or processed-leachate (salinity 19.6%o) from sanitary landfill sites. The relative biomass increase rate of p. australis was significantly higher than Z. latifolia and T. angustifolia. In the case of processed-leachate treatment, the relative biomass increase rates of above-part, rhizome and root of P. australiswere 178 %, 148 % and 157 %, respectively. Also, in 15%o NaCl-salt solution treatment, the relative biomass increase rates of P. australis increased as follows; 161 % (above-part), 183 % (rhizome) and 112 % (root). Total chlorophyll contents increased significantly in the leaves of P. australis and Z. latifolia grown in 15%o NaCl-salt solution and processed-leachate. Among three macrophytes, P. australis was evaluated as most effective macrophyte for the biological retreatment of processed-leachate from sanitary landfill sites.

• 한국환경복원기술학회지
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• 제19권1호
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• pp.13-23
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• 2016

This study was conducted to investigate the growth characteristics of warm-season turfgrasses and to find out suitable turfgrass species on Saemangeum reclaimed land. Twenty native zoysiagrass(Zoysia sinica, Z. matrella, Z. japonica, Medium-leaf type zoysiagrass(hybrid zoysiagrass)) and bermudagrass(Cynodon dactylon) collected from Korea were used in this study. Total stolon length and the number of stolon per square meter, relative growth rate of shoot and stolon, and coverage rate were analyzed for 2 year. C. dactylon showed not only the most growth response with high relative growth rate of shoot and stolon, which were 19.9% and 66.3%, but also resulted in higher level of turf visual quality compared to others. Whereas Z. japonica showed the least growth response with low relative growth rate of shoot and stolon, which were 2.4% and 0.7%. Although all warm-season turfgrasses took root and grew up well, there were different growth rates between the interspecies. Z. sinica 'Z2034', Z. matrella 'Z4091', Z. japonica 'Z1064', Medium-leaf type zoysiagrass 'ZN6019' and C. dactylon 'BN7014' were the greatest growth rate of shoot and stolon. These results will be useful for selecting salt tolerant breeding lines and also used to develop a turfgrass cultivar with strong salinity tolerance through continuous monitoring.

• 한국작물학회지
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• 제50권1호
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• pp.5-10
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• 2005

This experiment was conducted to clarify the functions of supernodulating characters on seed yield determination through the comparison of agricultural traits of supernodulating soybean mutants, Sakukei4, SS2-2, and their parent cultivars, Enrei and Shinpaldalkong2. The plant dry weights of supernodulating mutants, Sakukei4 and SS2-2, were $52\%$ and $61\%$ of their wild type parents at full seed stage (R6). However, the relative growth rate (RGR) from the pod set stage (R3) to R6 of Sakukei4 was 0.022 g/g/day and that of SS2-2 was 0.016 g/g/day, which were higher than those of their parents. Nodule number and dry weight were increased in two supernodulating mutants by the R6 stage. The nitrogen concentrations of leaf, petiole and stem of Sakukei4 were higher than those of Enrei. SS2-2 showed higher nitrogen concentration in petiole than Shinpaldalkong2 had. The positive correlations were appeared between nodule dry weight, plant dry weight and pod number, in two supernodulating mutants during the period from R3 to R6 stage. Although all of the yield components and seed yield were lower in two supernodulating mutants than their parents at the stage of full maturity (R8), the harvest index was higher in supernodulating mutants. The increasing rates of pod number to stem dry weight in two supernodulating mutants showed the higher than those of two their parents at R8 stage. In conclusion, the relative growth rates during the early to the middle reproductive growth period were higher in supernodulating mutants than the wild types. This could be resulted in an increase in pod number. The increase of relative growth rate was the result of the successive supplement of nitrogen source from biological nitrogen fixation (BNF) of nodules during the middle reproductive growth period in supernodulating mutants.

• 임산에너지
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• 제21권3호
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• pp.46-53
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• 2002

본 연구에서는 인공피음에 의해서 광조건을 3단계로 달리하여 생육시킨 펜둘라자작나무, 층층나무, 물푸레나무를 대상으로 생장량과 물질생산량 등 특성을 조사하여 생리ㆍ생태적 특성을 밝히고자 연구를 실시하였다. 인공피음처리에 따른 조사 대상 수종의 묘고생장 차이는 거의 없었으며, 근원직경의 생장은 전광처리구가 투광율 7-10%의 피음처리구보다 상대생장율이 29-32% 이상 우수한 생장율을 나타냈다. 하지만 층층나무의 근원직경생장은 투광율 26-34% 처리구에서 높은 생장율을 나타냈다. 대부분의 수종에서 피음처리 수준에 따른 광량감소와 함께 총 물질생산량도 감소하였고, 잎과 줄기의 비율이 뿌리에 비해 증가하는 경향을 보였다. 유묘의 T/R율은 대부분의 수종이 피음의 강도가 강할수록 T/R율이 높아지는 경향으로 전광처리구의 0.51∼l.13보다 투광율 7-10%의 강피음처리구가 1.00∼2.27로 높게 나타냈다. 전체적으로 비교할 때 인공피음처리에 의한 광량이 감소할수록 SLA, LAR, LWR은 광환경의 변화에 따른 피음의 강도가 강할수록 통계적으로 유의적인 차이를 보이며 증가하였다.

• Journal of Nutrition and Health
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• 제4권4호
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• pp.29-38
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• 1971

A survey on 4,117 (Boys:2,004, Girls:2,113) High School students in an urban area was conducted for assessing their physical growth, development and nutritional status from may throuth june 1971, and the following results were obtained. 1. Physical Growth and Development. a) Maximum growth was seen during the period from 12 to 16 years of age. b) The Maximum annual growth rates were as follows; Height: Boys-8.1cm from 14 to 15 years of age Girls-4.7cm from 13 to 14 Weight: Boys-8.0kg from 14 to 15 Girls-4.5kg from 13 to 14 Chest Girth: Boys-6.1cm from 14 to 15 Girls-4.2cm from 13 to 14 Sitting Height: Boys-4.6cm from 14 to 15 Girls-2.5cm from 13 to 14 c) The greatest individual differences in growth and development were observed during 12-15 years of age. d) Korean students were shorter than Japanese in 12-16 years of age group but after 17, Korean students become taller than Japanese. e) The standards of growth of rescent urban korean students show much improved than that of 1955. 2. Various Indices about Physical Growth and Development. a) Relative body weight was increased steadily annually and the crossing point of boys and girls appeared at age 11 at which girls surpassed boys and at 14, boys surpassed girls. b) Relative chestgirth showed narrow chest style in boys from 11 to 15 and from 11 to 14 in girls. c) The average relative sitting height was 54.0 in both sexes. d) The $R\ddot{o}hrer$ index was 1.1 in boys and 1.2-1.3 in girls. e) The Kaup index was lower than 2.0 in boys from 11 to 16 and in girls from 11 to 14. It becomes higher than 2.0 after 17 in boys but in girls after 15.

• 한국수산과학회지
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• 제48권5호
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• pp.704-711
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• 2015

We examined the effects of temperature and light intensity on gametophyte growth of Ecklonia cava Kjellman. The growth of female and male gametophytes was investigated before maturation. Gametophytes prepared from Munseom, Jeju, Korea in October 2014 were separated by sex and maintained under the following conditions: 15℃, 20 μmol·m^-2·s^-1, and a 12-h/12-h light/dark cycle. The experiments were conducted at five temperatures (10, 15, 20, 25, and 30℃) and three light intensities (5, 20, and 40 μmol·m^-2·s^-1). Daily growth rates were estimated by the increase in filamentous frond area. The optimal growth conditions for female gametophytes were 20℃ and 40 μmol·m^-2·s^-1; the optimal conditions for male gametophytes were 15℃ and 20 μmol·m^-2·s^-1. The relative growth rates (RGR) of female and male gametophytes decreased at 25℃ and 30℃, respectively. At 30℃, RGRs of gametophytes decreased by approximately 1.0% day^-1. In addition, RGRs of male gametophytes were higher than those of female gametophytes. These results indicate that female gametophytes were more sensitive to temperature and light intensity than male gametophytes. Moreover, these results suggest that E. cava growth rates could decrease as a result of global warming.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제31권2호
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• pp.137-142
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• 2005

To analyze the developmental changes in soma diameters of digastric motoneurons, wheat-germ agglutinin conjugated horseradish peroxidase (WGA-HRP) was injected into the digastric muscle and visualized the retrogradely HRP-labeled motoneurons through tungstate/tetramethylbenzidine (TMB) and following diaminobenzidine (DAB) reactions. The results obtained from Sprague-Dawley rats at postnatal days 1 (P1), 10 (P10) and 30 (P30) indicated as follows: firstly, soma diameters of digastric motoneurons showed unimodal distribution in all postnatal days examined; secondly, the period of P1 to P10 (period 1) showed about 2 times faster growth rate than that of P10 to P30 (period 2); thirdly, the smallest soma examined in each postnatal day exhibited slower growth rate with that of the largest one (increase ratio in soma diameters from P1 to P30, smallest vs. largest = 1.62 : 1.93); Finally, relative growth rates a day showed again that period 1 had faster growth rate than that of period 2. Consequently, developmental changes in soma diameters of digastric motoneurons resulted in very different growth rates between both periods. This implies that the growth of the soma is almost completing within P10 and thereafter growing slowly. The period 1 and 2 are corresponding to sucking and sucking/masticatory period, respectively. Therefore present study providing morphological changes in soma diameters of digastric motoneurons suggests that both periods and their different growth rates of the motoneurons in each period may closely be related with each other.

• 한국유기농업학회지
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• 제6권1호
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• pp.99-108
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• 1997

The aim of this experiment was to investigate the effect of applying times of cattle slurry on dry matter yield of Orchardgrass, when cattle slurry applied at rates of 30m3(average mineral nitrogen fertilizer equivalent to 120kg) per ㏊ in different dressing times, such as S1(1st and 2nd growth), S2(1st and 3rd growth), S3(1st and 4th growth), S4(2nd and 3rd growth), S5(2nd and 4th growth) and S6(application for 3rd and 4th growth). The results were as follows. 1. The average annual yields of dry matter were produced 6.36~7.42 ton per ㏊ in 1995~1997 when cattle slurry applied at rates of 30m3(average mineral nitrogen fertilizer equivalent to 120kg) per ㏊ in different dressing times, especially these tendency were shown higher annual dry matter in S4 plot9application for 2nd and 3rd growth). Those were higher 2.48~3.54 tons or 1.22~2.28 tons per ㏊ than the yields from non-fertilizing or phosphrous and potassium fertilizing. 2. Relative yields of annual dry matter in cattle slurry application plots were 164(S2 plot)~191(S4 plot)% in comparing with non-fertilizing plot. 3. Dry matter yields according to cutting frequency were highest at the 2nd cut(1.50 ton) and were in the order 3rd cut(0.95 ton)>4th cut(0.75 ton)>1st cut(0.69 ton/㏊). Also, those of cattle slurry application plot per ㏊ were in the order 2nd cut(2.02~2.73 tons)>3rd cut(1.56~2.08 tons)>4th cut(1.07~1.68 tons)>1st cut(1.11~1.45 tons/㏊). 4. Relative yields of annual dry matter in cattle slurry application plots were 164~219, 161~210, 143~212 and 135~182% at the 3rd, 1st, 4th and 2nd cut, respectively in comparing with non-fertilizing plot. 5. The efficiencies of nitrogen on dry matter yield(kg DM/kg N) were 18.1, 21.3 and 34.5kg DM/kg N when cattle slurry applied to Orchardgrass at rates of 30m3 (average mineral fertilizer equivalent to 120 kg) per ㏊ in 1993, 1994 and 1995, respectively, especially these tendency were shown higher efficiencies in S4 plot(application for 2nd 3rd growth). On the other hand, those of the same level fertilization of mineral nitrogen were 43.8, 19.2 and 13.4 kg DM/kg N in 1993, 1994 and 1995, respectively.

• 한국환경과학회지
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• 제33권6호
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• pp.383-402
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• 2024

Variations in lettuce growth and quality were observed depending on the type of artificial light source. The RGB LED treatment resulted in thick leaf development, leading to higher fresh weight, dry weight, and relative growth rates. Two cultivars, "Tomalin" and "seonpunggold," exhibited increased anthocyanin content and dark red leaf color under conditions of RGB LED treatment. Additionally, they exhibited high chlorophyll content under conditions of RGB LED and RGBFR LED treatments. Particularly, under Red LED treatment, the plants showed elongated leaves with narrow widths, resulting in a higher leaf shape index and a tendency towards leaf curling. Therefore, RGB LED lighting which appropriately blends red, blue, and green lights, is more effective than single lighr sources at improving lettuce growth and quality.