• Journal of Microbiology and Biotechnology
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• v.18 no.9
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• pp.1510-1517
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• 2008

Expresssed sequence tag (EST) analysis was developed from three cDNA libraries constructed from cells of the digestive tract, gonad, and liver of sea squirt. Randomly selected cDNA clones were partially sequenced to generate a total of 922 ESTs, in which 687 unique ESTs were identified respectively. Results of BLASTX search showed that 612 ESTs (89%) have homology to genes of known function whereas 75 ESTs (11%) were unidentified or novel. Based on the major function of their encoded proteins, the identified clones were classified into ten broad categories. We also identified several kinds of immune-related genes as identifying novel genes. Sequence analysis of ESTs revealed the presence of microsatellite-containing genes that may be valuable for further gene mapping studies. The accumulation of a large number of identified cDNA clones is invaluable for the study of sea squirt genetics and developmental biology. Further studies using cDNA microarrays are needed to identify the differentially expressed transcripts after disease infection.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.2
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• pp.313-319
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• 2009

Recently the RFID system, which can recognize multiple tags simultaneously through wireless communication, is emerging as a new technology that can replace the barcode system. Furthermore, related industries are carrying out active research on tags and authentication protocols with guaranteed security that are widely applicable to logistics, distribution, etc. The present study proposes a protocol with enhanced security by introducing the concept of RBAC to the authentication protocol, and a method with lower security for effective mass authentication. The proposed method is advantageous in that it guarantees security against spoofing attack, traffic analysis, replay attack, etc. based on hash function.

• Journal of Microbiology and Biotechnology
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• v.21 no.4
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• pp.347-358
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• 2011

Fomitopsis palustris, a brown-rot basidiomycete, causes the most destructive type of decay in wooden structures. In spite of its great economic importance, very little information is available at the molecular level regarding its complex decay process. To address this, we generated over 3,000 expressed sequence tags (ESTs) from a cDNA library constructed from F. palustris. Clustering of 3,095 high-quality ESTs resulted in a set of 1,403 putative unigenes comprising 485 contigs and 918 singlets. Homology searches based on BlastX analysis revealed that 78% of the F. palustris unigenes had a significant match to proteins deposited in the nonredundant databases. A subset of F. palustris unigenes showed similarity to the carbohydrateactive enzymes (CAZymes), including a range of glycosyl hydrolase (GH) family proteins. Some of these CAZyme-encoded genes were previously undescribed for F. palustris but predicted to have potential roles in biodegradation of wood. Among them, we identified and characterized a gene (FpCel45A) encoding the GH family 45 endoglucanase. Moreover, we also provided functional classification of 473 (34%) of F. palustris unigenes using the Gene Ontology hierarchy. The annotated EST data sets and related analysis may be useful in providing an initial insight into the genetic background of F. palustris.

• Fisheries and Aquatic Sciences
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• v.12 no.4
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• pp.286-292
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• 2009

We have constructed a cDNA library using brain samples of olive flounder Paralichthys olivaceus. Here, we described the study on gene identification by screening 356 clones from the brain cDNA library of olive flounder. Here, we screened 356 clones from the library to identify genes. Of these, 176 (49.5%) were identified as orthologs of known genes from olive flounder and other organisms. Among the 176 EST clones, 33 (18.7%) represented 11 unique genes that are identical to expressed sequence tags (ESTs) reported for olive flounder, and 120 (68.2%) represented 102 unique genes known from other organisms. The percentage of unknown genes (50.5%) is higher than in other olive flounder cDNA libraries (Lee et al., 2003, 2006, 2007), reflecting the high complexity of brain tissue. Further studies of expression characterization and developmental behavior related to these genes should provide useful insight into the physiological functions of the brain in olive flounder.

• Journal of KIBIM
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• v.7 no.1
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• pp.45-53
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• 2017

Continuous research efforts have been made on acquiring location data on construction sites. As a result, GPS and RFID are increasingly employed on the site to track the location of equipment and materials. However, these systems are based on radio frequency technologies which require attaching tags on every target entity. Implementing the systems incurs time and costs for attaching/detaching/managing the tags or sensors. For this reason, efforts are currently being made to track construction entities using only cameras. Vision-based 3D tracking has been presented in a previous research work in which the location of construction manpower, vehicle, and materials were successfully tracked. However, the proposed system is still in its infancy and yet to be implemented on practical applications for two reasons. First, it does not involve entity matching across two views, and thus cannot be used for tracking multiple entities, simultaneously. Second, the use of a checker board in the camera calibration process entails a focus-related problem when the baseline is long and the target entities are located far from the cameras. This paper proposes a vision-based method to track multiple workers simultaneously. An entity matching procedure is added to acquire the matching pairs of the same entities across two views which is necessary for tracking multiple entities. Also, the proposed method simplified the calibration process by avoiding the use of a checkerboard, making it more adequate to the realistic deployment on construction sites.

• Journal of Institute of Control, Robotics and Systems
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• v.12 no.1
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• pp.41-46
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• 2006

Ubiquitous location based services, offer helpful services anytime and anywhere by using real-time location information of objects based on ubiquitous network. Particularly, autonomous mobile robots can be a solution for various applications related to ubiquitous location based services, e.g. in hospitals, for cleaning, at airports or railway stations. However, a meaningful and still unsolved problem for most applications is to develop a robust and cheap positioning system. A typical example of position measurements is dead reckoning that is well known for providing a good short-term accuracy, being inexpensive and allowing very high sampling rates. However, the measurement always has some accumulated errors because the fundamental idea of dead reckoning is the integration of incremental motion information over time. The other hand, a localization system using RFID offers absolute position of robots regardless of elapsed time. We construct an absolute positioning system based on RFID and investigate how localization technique can be enhanced by RFID through experiment to measure the location of a mobile robot. Tags are placed on the floor at 5cm intervals in the shape of square in an arbitrary space and the accuracy of position measurement is investigated . To reduce the error and the variation of error, a weighting function based on Gaussian function is used. Different weighting values are applied to position data of tags since weighting values follow Gaussian function.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.122-122
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• 2003

Expressed sequence tags (EST) are help to quickly identify functions of expressed genes and to understand the complexity of gene expression. In order to analyze gene expression of the leaf development in Panax ginseng, which is one of the most important medicinal plant, expressed sequence tags (EST) analysis was carried out. We constructed a cDNA library using the immature leaf of Chunpoong. Partial sequences were obtained from 3,170 clones. The ESTs could be clustered into 1,624 (56.1%) non-redundant groups. Similarity search of the non-redundant ESTs against public non-redundant databases of both protein and DNA indicated that 1,137 groups show similarity to genes of known function. These ESTs clones were divided into sixteen categories depending upon gene function. Most abundant transcripts in immature ginseng leaf were photosynthesis related protein, such as chlorophyll a/b binding protein LHCII type I (128), chlorophyll a/b binding protein (53), ribulose-1,5-bisphosphate carboxylase (41), and photosystem I psaH (26). The EST data from immature leaf generated in this study is useful in dissecting gene expression in leaf organ of ginseng.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.123-123
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• 2003

In order to study gene expression transcribted during the embryo development, we constructed a cDNA library of embryogenic callus induced from cotylendon of Korean ginseng and generated expressed sequence tags (ESTs) of 3,359 clones randomly selected. The ESTs could be clustered into 1,910 (59.1%) non-redundant groups. Similarity search of the non-redundant ESTs against public non-redundant databases of both protein and DNA indicated that 2,217 groups show similarity to genes of known function. These ESTs clones were divided into eighteen categories depending upon gene function. Most abundant transcripts were ribosomal protein small subunit 28kDa(40), tumor-related protein(35), metallothionein (31), small heat-shock protein class 18.6K(24), and cyclophilin(20). There are no useful informations of gene expression during the embryo development in Korean ginseng. These results could help to understand the embryo development in Korean ginseng.

• Journal of Internet Computing and Services
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• v.17 no.6
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• pp.133-141
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• 2016

Recent web services provide tagging function to users, and let them express the topic of the contents of their articles. Moreover, we can extract context information like emotion of the writer efficiently by using tags attached to the articles or images. And we are able to better understand article than traditional algorithm. (eg. TF-IDF) Therefore, if we use tags in recommendation system, we can recommend high quality resources to the users. This study proposes a recommendation method that provide web resources (articles, users) through simple algorithm based on related tag set extracted from the article. Through the experiments, we show that the result was satisfactory, and we measure the satisfaction of users.

• Journal of Information Technology Applications and Management
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• v.30 no.6
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• pp.1-15
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• 2023

With the advent of the Fourth Industrial Revolution, propelled by digital technology, we are transitioning into an era of hyperconnectivity, where everything and objects are becoming interconnected. A smart supply chain refers to a supply chain system where various sensors and RFID tags are attached to objects such as machinery and products used in the manufacturing and transportation of goods. These sensors and tags collect and analyze process data related to the products, providing meaningful information for operational use and decision-making in the supply chain. Before the spread of COVID-19, the fundamental principles of supply chain management were centered around 'cost minimization' and 'high efficiency.' A smart supply chain overcomes the linear delayed action-reaction processes of traditional supply chains by adopting real-time data for better decision-making based on information, providing greater transparency, and enabling enhanced collaboration across the entire supply chain. Therefore, in this study, a hierarchical model for building a smart supply chain was constructed to systematically derive the importance of key factors that should be strategically considered in the construction of a smart supply chain, based on the major factors identified in previous research. We applied AHP (Analytical Hierarchy Process) techniques to identify urgent improvement areas in smart SCM initiatives. The analysis results showed that the external supply chain integration is the most urgent area to be improved in smart SCM initiatives.