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Isolation and Characterization of Inducible Genes from Bombyx mori Injected with E. coli by Differential Screening (누에에의 차별화 선별을 통한 면역 관련 유도 유전자의 분리와 특성)

  • 김상현;제연호
    • Journal of Sericultural and Entomological Science
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    • v.38 no.1
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    • pp.19-24
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    • 1996
  • To investigate the genes which is related to immune reaction of Bombyx mori, differential screening was carried out using naive and induced B. mori mRNA probe. To begin with, we constructed the cDNA library with mRNA isolated from fifth instar larvae injected with E. coli(4 X 106 cells/larva) using Uni ZAP XR vector kit. Thirty-two inducible cDNAs showing higher intensity on the induced mRNA probing membranes were selected. Partial nucleotide sequences of 29 clones were determined and their expessed sequence tags (ESTs) were produced. Nineteen ESTs in 29 ESTs were matched in GenBank database and the rest of them were found to be unknown. These unmatched ESTs were presumed to be novel genes. The nineteen ESTs contained variable genes related to biological process in Bombyx mori and four classes immune genes. Four clones, BmInc 6, 8, 18 and 27 were similar to two antibacterial peptide genes, hemolin gene and transferrin gene, respectively.

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Smart Browser based on Semantic Web using RFID Technology (RFID 기술을 이용한 시맨틱 웹 기반 스마트 브라우저)

  • Song, Chang-Woo;Lee, Jung-Hyun
    • The Journal of the Korea Contents Association
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    • v.8 no.12
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    • pp.37-44
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    • 2008
  • Data entered into RFID tags are used for saving costs and enhancing competitiveness in the development of applications in various industrial areas. RFID readers perform the identification and search of hundreds of objects, which are tags. RFID technology that identifies objects on request of dynamic linking and tracking is composed of application components supporting information infrastructure. Despite their many advantages, existing applications, which do not consider elements related to real.time data communication among remote RFID devices, cannot support connections among heterogeneous devices effectively. As different network devices are installed in applications separately and go through different query analysis processes, there happen the delays of monitoring or errors in data conversion. The present study implements a RFID database handling system in semantic Web environment for integrated management of information extracted from RFID tags regardless of application. Users’ RFID tags are identified by a RFID reader mounted on an application, and the data are sent to the RFID database processing system, and then the process converts the information into a semantic Web language. Data transmitted on the standardized semantic Web base are translated by a smart browser and displayed on the screen. The use of a semantic Web language enables reasoning on meaningful relations and this, in turn, makes it easy to expand the functions by adding modules.

Analyses of Security, Privacy Issues and Challenges for RFID System

  • Kim, Jung-Tae
    • Journal of information and communication convergence engineering
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    • v.9 no.6
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    • pp.701-705
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    • 2011
  • RFID is a widely adopted in the field of identification technology these days. Radio Frequency IDentification (RFID) has wide applications in many areas including manufacturing, healthcare, and transportation. Because limited resource RFID tags are used, various risks could threaten their abilities to provide essential services to users. A number of RFID protocols have done by researcher in order to protect against some malicious attacks and threat. Existing RFID protocols are able to resolve a number of security and privacy issues, but still unable to overcome other security & privacy related issues. In this paper, we analyses security schemes and vulnerability in RFID application. Considering this RFID security issues, we survey the security threats and open problems related to issues by means of information security and privacy. Neither a symmetric nor an asymmetric cryptographic deployment is necessarily used with light weighted algorithm in the future.

Web-based Information Management for Korean Traditional Building Materials

  • Lee, Sang-Don;Lee, Sang-Il;Choi, Jong-Myung
    • International Journal of Contents
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    • v.5 no.3
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    • pp.14-18
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    • 2009
  • Traditional methods and materials used for Korean buildings need to be well organized and managed so that they can be utilized in modernization of old buildings. Supporting web-based management of information of Korean traditional building materials helps spread the related knowledge. This paper identifies the characteristics of traditional building materials data, and develops an information structure to represent the related information effectively. It also describes design decisions on web-based user interfaces to support flexible browsing and retrieval of the managed data. As the traditional building data are described by old domain-specific technical terms, utility of the developed service might be limited to those who are familiar with the terms. As an approach to tackle this problem, the proposed system supports user tagging by allowing users to classify the stored information using their own terms, and also to retrieve data using the user-supplied tags.

Transferability of Cupped Oyster EST (Expressed Sequence Tag)-Derived SNP (Single Nucleotide Polymorphism) Markers to Related Crassostrea and Ostrea Species

  • Kim, Woo-Jin;Jung, Hyungtaek;Shin, Eun-Ha;Baek, Ilseon
    • The Korean Journal of Malacology
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    • v.30 no.3
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    • pp.197-210
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    • 2014
  • Single nucleotide polymorphisms (SNPs) are widely acknowledged as the marker of choice for many genetic and genomic applications because they show co-dominant inheritance, are highly abundant across genomes and are suitable for high-throughput genotyping. Here we evaluated the applicability of SNP markers developed from Crassostrea gigas and C. virginica expressed sequence tags (ESTs) in closely related Crassostrea and Ostrea species. A total of 213 putative interspecific level SNPs were identified from re-sequencing data in six amplicons, yielding on average of one interspecific level SNP per seven bp. High polymorphism levels were observed and the high success rate of transferability show that genic EST-derived SNP markers provide an efficient method for rapid marker development and SNP discovery in closely related oyster species. The six EST-SNP markers identified here will provide useful molecular tools for addressing questions in molecular ecology and evolution studies including for stock analysis (pedigree monitoring) in related oyster taxa.

A Study of User Interests and Tag Classification related to resources in a Social Tagging System (소셜 태깅에서 관심사로 바라본 태그 특징 연구 - 소셜 북마킹 사이트 'del.icio.us'의 태그를 중심으로 -)

  • Bae, Joo-Hee;Lee, Kyung-Won
    • 한국HCI학회:학술대회논문집
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    • 2009.02a
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    • pp.826-833
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    • 2009
  • Currently, the rise of social tagging has changing taxonomy to folksonomy. Tag represents a new approach to organizing information. Nonhierarchical classification allows data to be freely gathered, allows easy access, and has the ability to move directly to other content topics. Tag is expected to play a key role in clustering various types of contents, it is expand to network in the common interests among users. First, this paper determine the relationships among user, tags and resources in social tagging system and examine the circumstances of what aspects to users when creating a tag related to features of websites. Therefore, this study uses tags from the social bookmarking service 'del.icio.us' to analyze the features of tag words when adding a new web page to a list. To do this, websites features classified into 7 items, it is known as tag classification related to resources. Experiments were conducted to test the proposed classify method in the area of music, photography and games. This paper attempts to investigate the perspective in which users apply a tag to a webpage and establish the capacity of expanding a social service that offers the opportunity to create a new business model.

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A Study on the Development of RFID based Automatic Gate Systems in Container Terminals (RFID 기반의 컨데이너터미널 게이트 자동화 시스템 개발에 관한 연구)

  • Lee Seok-Yong;Seo Chang-Gab;Par Nam-Kyu;Song Bok-Deuk
    • The Journal of Information Systems
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    • v.15 no.3
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    • pp.187-211
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    • 2006
  • As port competitiveness is becoming more important in the global market environment RFID (Radio Frequency Identification) Is also becoming a crucial enabler In implement efficient visible, secure and productive ports. However there Is a lack of practical validated RFID technology acceptance cases in the port logistics industry until now, even though various related projects have been undertaken. In this study, we applied 13.56MHz passive RFID readers, tags, and their applications into existing bar-code based gate systems to improve the port logistics process, and we analyzed results of a pilot test in economic and non-economic perspectives. The main purpose of this study is to develop the RFID based automatic gate passing system in container terminals, and is to validate its economic and non-economic feasibility. In order to accomplish the purpose of this study, first, we examined previous researches on RFID technology acceptance in the port logistics industry, second, we Identified and analyzed the business process of existing gate systems in container terminals, third, we build RFID gate systems with 13.56Mhz tags, readers, and its middle-ware, finally we tested the system and its performance. The results were successful and showed the feasibility of the system in real container terminal gates. Economic and non-economic contribution was confirmed. Although the system has technological limitations with short range passive type, we clearly identified its potential capability and its economic validity in the field, which are the implications of this study.

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Image Classification Using Bag of Visual Words and Visual Saliency Model (이미지 단어집과 관심영역 자동추출을 사용한 이미지 분류)

  • Jang, Hyunwoong;Cho, Soosun
    • KIPS Transactions on Software and Data Engineering
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    • v.3 no.12
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    • pp.547-552
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    • 2014
  • As social multimedia sites are getting popular such as Flickr and Facebook, the amount of image information has been increasing very fast. So there have been many studies for accurate social image retrieval. Some of them were web image classification using semantic relations of image tags and BoVW(Bag of Visual Words). In this paper, we propose a method to detect salient region in images using GBVS(Graph Based Visual Saliency) model which can eliminate less important region like a background. First, We construct BoVW based on SIFT algorithm from the database of the preliminary retrieved images with semantically related tags. Second, detect salient region in test images using GBVS model. The result of image classification showed higher accuracy than the previous research. Therefore we expect that our method can classify a variety of images more accurately.

An Experimental Study Investigating the Retrieval Effectiveness of a Video Retrieval System Using Tag Query Expansion (태그 질의 확장 기능에 기반한 비디오 검색 시스템의 효율성에 대한 실험적 연구)

  • Kim, Hyun-Hee
    • Journal of the Korean Society for Library and Information Science
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    • v.44 no.4
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    • pp.75-94
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    • 2010
  • This study designed a pilot system in which queries can be expanded through a tag ontology where equivalent, synonymous, or related tags are bound together, in order to improve the retrieval effectiveness of videos. We evaluated the proposed pilot system by comparing it to a tag-based system without tag control, in terms of recall and precision rates. Our study results showed that the mean recall rate in the structured folksonomy-based system was statistically higher than that in the tag-based system. On the other hand, the mean precision rate in the structured folksonomy-based system was not statistically higher than that in the tag-based system. The result of this study can be utilized as a guide on how to effectively use tags as social metadata of digital video libraries.

A Qualitative Exploration of Folksonomy Users' Tagging Behaviors (폭소노미에 따른 웹 분류 연구 - 이용자 태깅 행위 분석을 중심으로 -)

  • Park, Hee-Jin
    • Journal of the Korean Society for Library and Information Science
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    • v.45 no.1
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    • pp.189-210
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    • 2011
  • This study aims to explore how users are tagging in order to utilize a folksonomy and whether they understand the social and interactive aspects of tagging in three different folksonomic systems, Connotea (www.connotea.org), Delicious(http://delicious.com), and CiteULike(www.citeulike.org). The study uses internet questionnaires, qualitative diary studies, and follow-up interviews to understand twelve participants' tagging activities associated with folksonomic interactions. The flow charts developed from the twelve participants showed that tagging was a quite complex process, in which each tagging activity was interconnected, and a variety of folksonomic system features were employed. Three main tagging activities involved in the tagging processes have been identified: item selection, tag assignment, and tag searching and discovery. During the tag assignment, participants would describe their tagging motivations related to various types of tags. Their perception of the usefulness of types of tags was different when their purpose was for social sharing rather than personal information management. While tagging, participants recognized the social potential of a folksonomic system and used interactive aspects of tagging via various features of the folksonomic system. It is hoped that this empirical study will provide insight into theoretical and practical issues regarding users' perceptions and use of folksonomy in accessing, sharing, and navigating internet resources.