• Journal of Plant Biotechnology
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• v.2 no.3
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• pp.143-149
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• 2000

An Efficient in vitro regeneration system and an optimized Agrobacterium mediated transformation protocol are described, based on the use of young seedling cotyledons of Capsicum annuum L. Optimal regeneration efficiency can be obtained by cultivating cotyledon explants on media containing 4 mg/L benzyladenine and 0.1 mg/L indolacetic acid. The effect of antibiotics used to eliminate Agrobacteria, as well as the toxic level of some generally used selection agents (kanamycin, geneticin, hygromycin, phosphinotricin and methotrexate) in regenerating pepper tissues were determined. To enable the comparison of different selection markers in identical vector background, a set of binary vectors containing the marker genes for NPTII, HPT, DHFR and BAR respectively, as well as the CaMV 35S promoter/enhancer-GUS chimaeric gene was constructed and introduced into four different Agrobacterium host strains.

• 한국신재생에너지학회:학술대회논문집
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• 2008.05a
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• pp.341-344
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• 2008

Computational studies have been performed to analyze the particle loadings onto the filters according to the different inflow pattern of dusty gas and the flow uniformities on the filter surface of back-blown gas at different permeability of porous media setup at the filter outlet. This is preliminary study to find how we can reduce the regeneration time and improve the regeneration efficiency of filters with dust layer.

• Architectural research
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• v.12 no.1
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• pp.57-64
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• 2010

In recent years, interests and studies on the urban regeneration projects have been increased and largely conducted. It is a part of the effort that solves some social and economical problems occurred by deteriorated buildings and degraded infrastructures through new urban regeneration projects or redevelopment projects. However, the urban regeneration projects show the characteristics that can not guarantee in the project performance because the projects have various and complex stakeholders related to these projects and are exposed to lots of risks due to its huge scale. This study proposed the risk performance index method to improve the efficiency of the overall performance measurement for a mega-project by extending from the traditional cost/schedule based performance measurement system. The risk performance index method proposed in this study has a similar system to the EVMS, and makes possible to perform a three dimensional integrated performance measurement in cost/schedule/risk through 18 different indexes that compose the risk performance index.

• Journal of Power System Engineering
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• v.20 no.3
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• pp.36-42
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• 2016

A cylinder control system of the conventional construction machine has been controlled by hydraulic spool valves. This system is low-cost but system efficiency is not high. Recently, to improve this, all valves are controlled electronically and independently. Bu and Yao suggested four way electronic hydraulic control valve system. It is called IMVT(Independent Metering Valve Technology). The purpose of the study is to find proper IMV pressure control method for excavator and to validate excavator's bucket regeneration energy effect by controlling the IMV system. In this paper, we mathematically describe the bucket system of excavator first. And then, based on these results, we design the control system which is divided into two operations(none regeneration or regeneration).The results of the experiment show the desirable performance and usefulness of the designed control system.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.2
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• pp.49-52
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• 2001

conditions for callus induction and plant regeneratin from seeds of lawngrass (Zoysia japonica Steud.) were confirmed in this study. MS (Murashige and Skoog) medium containg 2,4-D 3 or 5mg/l was used for callus induction, and MS medium with different volumes of BA, NAA and kinetin hormones was used to regenerate the plants from induced calli. MS basic medium containing agar with no hormones or kinetin 1.0mg/l and MS basic medium containing gelite and NAA 1.0mg/l were higher for green callus induction. MS medium containing agar and kinetin 1.0mg/l ws highest degree of efficiency for plant regeneration.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2011.05a
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• pp.181-184
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• 2011

The earlier studies which are related to conflict management for urban regeneration project were based on limited case-studies on a specific phase. In addition, those studies did not considered a multidisciplinary approach for critical factor of conflict occurrence. In this reason, to establish a practical management or a policy is limited. In this paper, 'Good communication and information sharing' and 'Cooperativeness of stakeholders on project' are defined by critical factors for minimization of conflict between stakeholders. Furthermore we analysis the influence of those factors to minimization of conflict using path-analysis. This paper contributes to increase the efficiency of conflict management through understanding influence of each factors.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2004.05a
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• pp.104-109
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• 2004

Recently, when electric traction system used DC 1500[Vdc] runs on decline of rail road track and slows down, dc voltage goes beyond regular voltage. In this case extra power is forcibly wasted by resister because rectifier of substation and electric train including power converter and so on are out of order. Therefore this paper proposes that the extra power is regenerated through regeneration inverter to AC utility in result this system obstruct to go beyond regular voltage and improve the efficiency. In addition, electric traction system products harmonic current and voltage distortion and reactive power because power converter is used so regeneration inverter normally runs such as active power filter(APF) for improving power quality.

• Korean Journal of Food Science and Technology
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• v.16 no.3
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• pp.363-367
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• 1984

Conditions for efficient formation and regeneration of protoplasts of Streptococcus lactis ATCC 11454 were investigated. Addition of 20mM DL-threonine into growth medium, growth phase and lysozyme concentration had significant effects on protoplast formation. Approximately, 20% regeneration efficiency was obtained by optimizing the medium composition and modifying the plating procedure.

• Korean Journal of Plant Resources
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• v.32 no.3
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• pp.237-243
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• 2019

Soybean is the one of recalcitrant legume species for shoot induction. Shoot regeneration via direct organogenesis was investigated in five soybean cultivars, 'Dawon', 'Pungsan', 'Daewon', 'Taekwang' and 'Chongdoo 1' by using cotyledonary node explants. Out of 5 soybean cultivars, an efficient shoot regeneration condition was developed in the two soybean cultivars, 'Dawon' and 'Pungsan'. When various kinds of plant growth regulators with different concentration were estimated, the optimum medium condition for shoot induction in both soybean cultivars was MS + B5 vitamin supplemented with BA at concentration 2 mg/L. In addition, shoot formation efficiency was increased with 97.09% and 93.88% by the pretreatment of BA onto the explants before in vitro culture in both cultivars. Shoot induction in 'Dawon' cultivar was originated from epidermal tissue and sub-epidermal layers when histological changes were investigated under shoot regeneration after culturing cotyledonary node segments on shoot induction medium for 0 to 21 days. Especially, cell dedifferentiation was observed from parenchyma cells to meristematic cell in 3-day cultured segments.

• International conference on construction engineering and project management
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• 2009.05a
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• pp.1591-1598
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• 2009

Recently, the researches on the urban regeneration projects have been performed very actively. It is a part of the effort that solves some social and economical problems occurred by deteriorated buildings and degraded infrastructures through new urban regeneration projects or redevelopment projects. However, the urban regeneration projects show the characteristics that can not guarantee in the project performance because the projects have various and complex stakeholders related to these projects and are exposed to lots of risks due to its huge scale. This study proposed the risk performance index method to improve the efficiency of the overall performance measurement for a mega-project by extending from the traditional cost/schedule based performance measurement system. The risk performance index method proposed in this study has a similar system to the EVMS, and makes possible to perform a three dimensional integrated performance measurement in cost/schedule/risk through 18 different indexes that compose the risk performance index.