• Journal of Life Science
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• v.27 no.1
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• pp.44-49
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• 2017

Natural anti-oxidative compounds have important disease prevention and food preservation properties, in addition to anti-bacterial, anti-inflammation, anti-cancer, and skin whitening effects. High-performance liquid chromatography (HPLC), with an ultra vilolet (UV) detector coupled to a reverse phase C18 column and an online measurement system for 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radicals, was used to search for potent antioxidative compounds in crude extracts. The online HPLC-DPPH assay was then applied to confirm antioxidative compounds in water extracts from Radix of Pueraria lobata, Rhizoma of Zingiber officinale, Fructus of Chaenomeles sinensis, Cortex of Ulmus pumila, and Radix of Astragalus membranaceus. To determine the yields of the extracts, the Brix% of each extract solution was measured using a refractometer. When the relative DPPH radical scavenging ability values of the water extracts were compared with those of a positive control (ascorbic acid), the water extracts of P. lobata, C. sinensis, and U. pumila were 7.77%, 4.71%, and 4.19%, respectively. The results suggest that this method provides a useful assay for rapid measurement of DPPH radical scavenging abilities and conformation of antioxidative compounds in natural products. Moreover, it can reduce the time spent on the separation of active compounds from natural materials, such as medicinal plants, in addition to the use of reagents for separation.

• Journal of Korean Ophthalmic Optics Society
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• v.16 no.2
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• pp.187-193
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• 2011

Purpose: This study was to compare differences between both eyes in corneal powers, axial lengths, anterior chamber depths in anisometropia and isometropia, and to investigate the relationship between anisometropia and refractive components. Methods: The subject was a total of 83 patients, anisometropia 45 patients (90 eyes) and isometropia 38 patients (76 eyes) from 2.7 to 15.3 years old, prescribed eyeglasses and contact lenses by refraction from July 2010 to August 2010 in Gwangju City B eye clinic. Axial length, anterior chamber depth, corneal curvature, and corneal refractive power were measured using IOL Master. Refractive error was measured using an Auto-refractometer. Results: Anisometropia was a statistically significant difference in axial length, binocular refractive components, refractive error, and axial length, Axial length/corneal radius (AL/CR) ratio showed a statistically significant difference in anisometropia and isometropia. The major cause of anisometropia all 45 subjects was the axial length. Among the refractive components axial length, AL/CR had a strong correlation, but corneal refractive power had no correlation. Anterior chamber depth had a weak correlation. Conclusions: This study found that refractive error was the most axial ametropia caused by the axial length. The main cause of anisometropia was the axial length, but refractive components had a weak correlation.

• Journal of Korean Ophthalmic Optics Society
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• v.17 no.1
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• pp.75-81
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• 2012

Purpose: The study tried to figure out accommodative changes by measuring accommodative response, appearing on the full vision correction and low vision correction, with both eyes open-view auto-refractometer (Nvision-K5001, Shin-nippon, Japan). Methods: It carried out objective and subjective refractions, targeting 79 college students (58 males and 21 females) aged between 20 and 30($21.14{\pm}2.00$), by measuring accommodative changes with fixation distance at 1.0 m for eyesight of 1.0 after full version correction. The distances of 5.0 m, 1.0 m, 0.50 m, 0.33 m and 0.25 m for eyesight of 0.8, 0.7 and 0.6 after low vision correction arbitrarily added plus lens were applied. Results: the shorter measure fixation distances were, the greater changes accommodative response showed a tendency in the state of both full vision correction and low vision correction(0.7). The state of full vision correction showed a greater change of accommodative response than that of low vision correction(0.7). Both right and left eyes showed low accommodative responses in the state of low vision correction(0.7) than that of full vision correction. As a result of analyzing accommodative response at an eyesight of 0.8, 0.7, and 0.6 after low vision correction, the poorer eyesight was the lower accommodative response. Conclusions: Low vision correction from a near distance is expected to avoid unnecessary accommodative response, make eyes relaxed and prevent accommodative function disorder.

• Journal of Korean Ophthalmic Optics Society
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• v.19 no.1
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• pp.79-85
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• 2014

Purpose: This study investigated accommodative changes by measuring accommodative response, appearing on the normal and convergence insufficiency Group, by using both eyes open-view auto-refractometer (Nvision-K5001, shin-nippon, Japan). Methods: It carried out objective and subjective refractions, targeting 74 college students (54 males and 20 females) aged between 19 and 29 ($21.59{\pm}2.53$), spherical equivalent OD $-2.28{\pm}2.03$ D, OS $-2.18{\pm}2.01$ D, by measuring accommodative responses at full correction and under correction with plus lens +0.25, +0.50, +0.75 arbitrarily added. Results: In the group of normal and convergence insufficiency, the shorter fixation distances were, the greater accommodative lags showed. The group of convergence insufficiency showed the lesser changes of accommodative response than those of normal. But we found that the convergence insufficiency group had a little larger accommodative amplitude in the total fixation distances. The full correction of convergence insufficiency group and the under correction (+0.50 D) of normal were alike in the accommodative responses. We have also investigated that the correlation between accommodative responses and fixation distances was decreased steeply at the excessive low vision correction. Conclusions: Under correction (+0.50 D) in a near distance is expected to avoid unnecessary accommodative responses, make eyes relaxed and comfortable.

• Korean Journal of Food Science and Technology
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• v.48 no.1
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• pp.48-53
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• 2016

The objective of this study was to evaluate the accuracy and efficiency of different methods used for the fermentable sugar assay in the production of Makgeolli sul-dut. In the initial stage of fermentation, Ipguk treatment produced a higher alcohol content compared to the Nuruk treatment. However, the alcohol content was not significantly different between the two starters at the final stage of fermentation. Acidity in the Ipguk treatment was higher than that of Nuruk throughout the fermentation period. After analyzing the fermentable sugars using dinitrosalicylic acid (DNS), Fehling's method, refractometer, glucose kit, and high performance liquid chromatography (HPLC), it was confirmed that the HPLC method was the most accurate for fermentable sugar quantification. In both types of starters, DNS and Fehling's methods showed results comparable to HPLC in terms of fermentable sugar content, while the glucose kit and refractometer analyses showed relatively large discrepancies, indicating that the Fehling's method could also be effective for the analysis of fermentable sugars in the manufacture of Makgeolli.

• Korean Journal of Pharmacognosy
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• v.15 no.3
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• pp.139-146
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• 1984

High performance liquid chromatographic separation is described for the analysis of bile acids after hydrolysis in seven commercial crude bile drugs and ox and pig galls. They are simultaneously separated with HPLC mobile phase of acetonitrile/0.5% ammonium carbonate (pH 6.7) (25.5 : 74.5) at a flow rate $(1.0{\rightarrow}1.5ml/min.)$ and differential refractometer. The linearity of calibration curve and recovery test are good by using the method. The analysis of major bile acids in seven commercial crude bile drugs using the described method is presented. Sample no. 1 of them is similar to separation pattern of ox gall. Sample no. 6 of them is supposed to be genuine bear gall on the basis of identification of ursodeoxycholic acid. Sample $no.\;2{\sim}5$ and 7 of them are supposed to be pig gall on the basis of identification of hyodeoxycholic acid which is a characteristic component of pig gall.

• Proceedings of the Korean Information Science Society Conference
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• 2001.10b
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• pp.430-432
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• 2001

일반적으로 안과 의사들과 안경사 등을 포함하는 시각 관련 전문가들은 시력 피검자에 대한 굴절력 측정시 약 30% 이상의 오류율을 가진다고 한다. 이는 검사를 행하는 전문가의 비 숙련도에 기인한다기보다는 그만큼 시각 관련 측정이 유동적이고 민감해서 정확한 측정값을 얻어내는 것이 어렵다는 것을 말해주는 것이다. 만약 자동화된 시스템이 광학계로부터 나오는 영상을 이용하여 내부 처리를 거친 후 정확한 시각 측정치를 검사자에게 알려줄 수 있다면 잘못 측정되는 측정 횟수를 크게 줄일 수 있을 것이다. 본 연구는 형태학적 필터링(morphological filtering)과 그레이-레벨의 신호 강조(signal enhance) 기술들을 이용한 자동 시각 굴절력 측정 시스템을 개발하였다. 본 시스템에서는 광학계로부터 도출된 영상으로부터 첫째로 형태학적 필터링 처리를 행한다. 이 과정은 처리가 어려운 원 영상을 좀 더 다루기 쉬운 상태로 바꿔주는 역할을 하게 된다. 둘째는 일차 처리된 영상에 가해주는 그레이 수준 한계 기법을 통한 신호 강조 기법으로서 이는 영상의 그레이 값 분포가 다양함으로 인해서 발생되는 오차를 줄이기 위해서 사용된다. 그리하여 본 시스템은 정확한 측정값 도출이 어려운 시각 영상에 적용되어 효과적으로 오차를 줄임으로써 보다 효율적인 시각 측정을 가능하게 하였다.

• The Journal of Korean Medicine
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• v.24 no.2
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• pp.109-120
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• 2003

Objectives: This study was conducted to evaluate the clinical efficacy of EC102, a composition containing oriental medicine, for relieving VDT syndrome, including eye symptoms and musculo-skeletal symptoms. Methods: The study was conducted by using a double blind randomized controlled trial design. 40 subjects were randomly assigned to the placebo group or the EC102 group. The visual analogous scale (VAS) was used to evaluate various VDT symptoms, including eye pain, eye fatigue, back pain, shoulder pain and so on. Refractory indexes were measured using refractometer, and tear film break-up time (BUT) were measured using fluorescein strip. Blood circulation was measured using photo-plethysmography. Blood pressure and blood biochemistry were also measured. Statistical analysis was conducted by a Mann-Whitney test using SPSS 9.0 software. Results: The EC102 group showed a statistically significant reduction of eye symptoms including eye fatigue, eyelid fatigue, itching, pain, and photophobia when compared with the placebo group (P<0.05). The EC102 group also showed a statistically significant reduction of back pain compared with the placebo group (P<0.05). No side effects on liver function and blood biochemistry were observed. Conclusions: EC102 has significant clinical efficacy for relieving symptoms of VDT syndrome.

• Journal of Korean Ophthalmic Optics Society
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• v.12 no.4
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• pp.107-110
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• 2007

Analysis of accommodation amplitude has been conducted for 137 adults, living in rural area, aged from their fifties to eighties. The methods included measurement of correction power for distance via auto-refractometer, measurement of near power based on trial frame and trial lens set, and calculation of effective accommodation amplitude using 1/2 and 2/3 of the maximum accommodation amplitude. The research results for participants aged in their fifties, sixties, seventies, and eighties showed 1.84~2.46D, 1.55~2.06D, 1.43~1.90D, and 1.22~1.62D respectively. It is relatively higher than the existing knowledge regarding accommodation amplitude associated with age.

• Journal of Biosystems Engineering
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• v.23 no.6
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• pp.561-570
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• 1998

The MLR(Multiple Linear Regression) models to estimate soluble solids content non-destructively were presented to make a selection of optimal photosensor utilized to measure the soluble solids content of apples. Visible and NIR absorbance in the 400 to 2498 nanometer(nm) wavelength region, soluble solids content(sugar content), hardness, and weight were measured for 400 apples(gala). Spectrophotometer with fiber optic probe was utilized for spectrum measurement and digital refractometer was used for soluble solids content. Correlation between absorbance spectrum and soluble solids content was analyzed to pick out the optimal wavelengths and to develop corresponding prediction model by means of MLR. For the coefficient of determination($R^2$) to be over 0.92, the MLR models out of the original absorbance were built based on 7 wavelengths of 992, 904, 1096, 1032, 880, 824, 1048nm, and the ones of the second derivative absorbance based on 5 wavelengths of 784, 1056, 992, 808, 872nm. The best model of the second derivative absorbance spectrum had $R^2$=0.91, bias= -0.02bx, SEP=0.28bx for unknown samples.