• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.153-165
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• 2010

Brassica rapa is considered an ideal candidate to act as a reference species for Brassica genomic studies. Among the three basic Brassica species, B. rapa (AA genome) has the smallest genome (529 Mbp), compared to B. nigra (BB genome, 632 Mbp) and B. oleracea (CC genome, 696 Mbp). There is also a large collection of available cultivars of B. rapa, as well as a broad array of B. rapa genomic resources available. Under international consensus, various genomic studies on B. rapa have been conducted, including the construction of a physical map based on 22.5X genome coverage, end sequencing of 146,000 BACs, sequencing of >150,000 expressed sequence tags, and successful phase 2 shotgun sequencing of 589 euchromatic region-tiling BACs based on comparative positioning with the Arabidopsis genome. These sequenced BACs mapped onto the B. rapa genome provide beginning points for genome sequencing of each chromosome. Applying this strategy, all of the 10 chromosomes of B. rapa have been assigned to the sequencing centers in seven countries, Korea, UK, China, India, Canada, Australia, and Japan. The two longest chromosomes, A3 and A9, have been sequenced except for several gaps, by NAAS in Korea. Meanwhile a China group, including IVF and BGI, performed whole genome sequencing with Illumina system. These Sanger and NGS sequence data will be integrated to assemble a draft sequence of B. rapa. The imminent B. rapa genome sequence offers novel insights into the organization and evolution of the Brassica genome. In parallel, the transfer of knowledge from B. rapa to other Brassica crops would be expected.

• Mass Spectrometry Letters
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• v.2 no.2
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• pp.33-36
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• 2011

The misuse of anabolic androgenic steroids is of particular concern in sports and society. Thus, it is of great importance to discriminate endogenous steroids such as testosterone or testosterone prohormones from their chemically identical synthetic copies. In this study, gas chromatography-combustion/isotope ratio mass spectrometric (GC-C/IRMS) method has been developed and validated for discriminating the origin of anabolic androgenic steroids. The method involves the solid-phase extraction, enzymatic hydrolysis with ${\beta}$-glucuronidase, HPLC-fractionation for the cleanup and analysis by GC-C/IRMS. The difference(${\Delta}^{13}C$) of urinary ${\delta}^{13}C$ values between synthetic analogues and endogenous reference compounds (ERC) by GC-C/IRMS was used to elucidate the origin of steroids, and intra- and inter-day precision, specificity and isotope fractionation were evaluated. The present GC-C/IRMS method combined with HPLC cleanup was accurate and reproducible enough to be successfully applied to the test of urine sample from suspected anabolic steroid abusers.

• Analytical Science and Technology
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• v.12 no.3
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• pp.218-223
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• 1999

A single use, screen-printed sensor for the measurement of liquid phase ethanol was developed and its electrochemical performance was investigated. Disposable type edthanol sensor was fabricated by serially screen printing the carbon paste, silverd pasted and insulator inlon a polyester substrate to pattern working and reference electrode sites and electrical contact. Alcohol dehydrogenase(ADH) or alcohol oxidase(AOD) together with appropriate electron transfer mediators was immobilized on the working electrode. To improve the sensitivity and reproducibility of carbon paste electrode, some pretreatment procedures were applied and their resultant electrochemical performance was examined. The disposable type electrochemical ethanol sensor developed in this study conveniently determines the ethanol in liquid samples such as blood and in fermentation process.

• Journal of Pharmaceutical Investigation
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• v.39 no.4
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• pp.309-314
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• 2009

An enzyme immunoassay (EIA) was validated for quantitation of cacitriol in human plasma. Calcitriol was immunoextracted with immunocapsules, which contain monoclonal antibodies to calcitriol linked to solid phase particles in suspension with a vitamin D binding protein inhibitor. Calcitrol was eluated and the eluates were evaporated under a gentle stream of nitrogen gas. The absorbance of analytes was determined using a microplate reader (reference wavelength 650 nm; measurement wavelength 450 nm). The method was specific and sensitive enough to detect as low as 6.5 pmol/L of calcitriol. Linear calibration range was 6.5-491 pmol/L with correlation coefficient greater than 0.99. The overall accuracy was in the range of 83.8 to 111.2% and precision C.V. (%) 0.99 to 8.47%. The recovery was approximately 100% and stability was confirmed during storage and sample preparation. The pharmacokinetic parameters were calculated by baseline subtraction because calcitriol is an endogenous material. Following oral dose of calcitriol, the mean AUC$_{24h}$ was 1038${\pm}$539 pmol/Lhr and C$_{max}$ of 128${\pm}$63.1 pmol/L was reached at 3.50${\pm}$1.07 hr. The mean t$_{1/2}$ of calcitriol was 5.13${\pm}$2.10 hr. The present EIA method was successfully applied to study bioavailability after oral administration of 2 ${\mu}$g of calcitriol in healthy Korean subjects.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.2 no.2
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• pp.120-133
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• 2008

This paper presents a novel approach for facial motion tracking and facial expression cloning to create a realistic facial animation of a 3D avatar. The exact head pose estimation and facial expression tracking are critical issues that must be solved when developing vision-based computer animation. In this paper, we deal with these two problems. The proposed approach consists of two phases: dynamic head pose estimation and facial expression cloning. The dynamic head pose estimation can robustly estimate a 3D head pose from input video images. Given an initial reference template of a face image and the corresponding 3D head pose, the full head motion is recovered by projecting a cylindrical head model onto the face image. It is possible to recover the head pose regardless of light variations and self-occlusion by updating the template dynamically. In the phase of synthesizing the facial expression, the variations of the major facial feature points of the face images are tracked by using optical flow and the variations are retargeted to the 3D face model. At the same time, we exploit the RBF (Radial Basis Function) to deform the local area of the face model around the major feature points. Consequently, facial expression synthesis is done by directly tracking the variations of the major feature points and indirectly estimating the variations of the regional feature points. From the experiments, we can prove that the proposed vision-based facial expression cloning method automatically estimates the 3D head pose and produces realistic 3D facial expressions in real time.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.16 no.2
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• pp.45-53
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• 2002

This paper presents an algorithm for the appropriate parameter selection of a power system stabilizer and power converters in two-area power systems with a series HVDC links. The method for PSS is one of the classical techniques by allocating properly poly-zero positions to fit as closely as desired the ideal phase lead and by changing the gain to produce a necessary damping torque. Proper parameter of power converters are obtained in order to have sufficient speed and stability margin to cope with changing reference values and disturbances based on the Root-locus technique. The small signal and transient stability studies using the PSS and power converters parameters obtained from these methods show that a natural oscillation frequency of the study case system is adequately damped. The simulation used in the paper was performed by the Power System Toolbox software program based on MATLAB.

• Korean Journal of Construction Engineering and Management
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• v.10 no.3
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• pp.102-110
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• 2009

Although several research efforts have been directed to fast-tracking to reduce the total delivery time, few researches have been studied on concurrent engineering in construction projects. The focus of fast-tracking is primarily on overlapping independent activity pairs. In comparison, the focus of concurrent engineering is on overlapping dependent activity pairs. Dependent activities are much harder to overlap successfully. This paper presents a simulation-based Concurrent Engineering methodology to optimize the overall duration of a set of design activities in a project by modelling key factors that determine the duration of individual activities and overlap between dependent activities. This methodology involves determining how much to overlap activities, how to decide which activities to overlap and the corresponding cost and time tradeoffs using a discrete event model solution. This simulation model, therefore, can be used as a reference on decision-making to define optimum point between time and cost.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.30 no.3
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• pp.165-173
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• 2007

A space launch vehicle system represents a typical example of large-scale multi-disciplinary systems, consisting of subsystems such as mechanical structure, electronics, control, telecommunication, propulsion, material engineering etc. A lot of cost is required to develop the launch vehicle system. A precise planning of R&D cost is very essential to make a success of the launch vehicle development program. Especially in the early development phase of a new space launch vehicle system, cost estimation techniques and analogy from past similar development data are very useful methods to estimate a development cost of the launch vehicle system. Now Korea Aerospace Research Institute is in charge of the KSLV-I (Korea Space Launch Vehicle-I) Program that is a part of Korea National Space program. KSLV-I Program is a national undertaking to develop launch capabilities to deliver science satellites of a 100kg-class into a low earth orbit. It is hereafter, going to plan to develop a new korean space launch vehicle. In this paper, first the development costs of well-known launch vehicles in the world are presented to provide a reference to make a development plan of a new launch vehicle. Second this paper introduces the present status of cost estimation applications at NASA. Finally this paper presents the results from application of a TRANSCOST, a parametric cost model, to derive a cost estimate of a new launch vehicle development, as an example.

• Journal of Ginseng Research
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• v.40 no.3
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• pp.220-228
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• 2016

Background: Among the various ginseng strains, Shizhu ginseng is endemic to China, mainly distributed in Kuandian Manchu Autonomous County (Liaoning Province, China); however, not much is known about the compounds (especially saponins) in Shizhu ginseng. Methods: A rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with MS/MS (UHPLC-MS/MS) method was developed to separate and identify saponins in Shizhu ginseng. Results: The separation was carried out on a Waters ACQUITY UPLC BEH $C_{18}$ column ($100mm{\times}2.1mm$, $1.7{\mu}m$) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phase under a gradient elution at $40^{\circ}C$. The detection was performed on a Micromass Quattro Micro API mass spectrometer equipped with electrospray ionization source in both positive and negative modes. Under the optimized conditions, a total of 31 saponins were identified or tentatively characterized by comparing retention time and MS data with related literatures and reference substances. Conclusion: The developed UHPLC-MS/MS method was suitable for identifying and characterizing the chemical constituents in Shizhu ginseng, which provided a helpful chemical basis for further research on Shizhu ginseng.

• Membrane Journal
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• v.8 no.3
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• pp.138-147
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• 1998

This paper compared selectivities and mass transfer rates of valuable aromatics (naphthalene group: carbon number 10-12) in the light cycle oil obtained from solvent extraction (SE) with those obtained from liquid membrane permeation (LMP). An aqueous solution of dimethylsulfoxide (DMSO) and an aqueous solution of saporrin and DMSO were used as extraction solvent of SE and the membrane phase of LMP, respectivdy. Selectivities of naphthalene group in reference to n-nonane obtained from SE runs were rapidly increased with decreasing the operating temperature, whereas, those obtained from LMP runs were remained constant throughout the operating temperature. At room temperature, selectivities of naphthalene group obtained from SE were greater than those from SE. Furthermore, mass transfer rates of naphthalene group by SE and LMP were measured in a baffled batch stirred vessel. It was found that the extraction rates of SE were faster by about 280 times than the permeation rates of LMP.