• 제목/요약/키워드: Reference condition

검색결과 1,256건 처리시간 0.029초

Selection of suitable reference gene for gene expression studies of porcine ovaries under different conditions in quantitative reverse transcription polymerase chain reaction assay

  • Kim, Hwan-Deuk;Jeon, Hye-Jin;Jang, Min;Bae, Seul-Gi;Yun, Sung-Ho;Han, Jee-Eun;Kim, Seung-Joon;Lee, Won-Jae
    • 한국동물생명공학회지
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    • 제37권2호
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    • pp.96-105
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    • 2022
  • The ovary undergoes substantial physiological changes along with estrus phase to mediate negative/positive feedback to the upstream reproductive tissues and to play a role in producing a fertilizable oocyte in the developing follicles. However, the disorder of estrus cycle in female can lead to diseases, such as cystic ovary which is directly associated with decline of overall reproductive performance. In gene expression studies of ovaries, quantitative reverse transcription polymerase chain reaction (qPCR) assay has been widely applied. During this assay, although normalization of target genes against reference genes (RGs) has been indispensably conducted, the expression of RGs is also variable in each experimental condition which can result in false conclusion. Because the understanding for stable RG in porcine ovaries was still limited, we attempted to assess the stability of RGs from the pool of ten commonly used RGs (18S, B2M, PPIA, RPL4, SDHA, ACTB, GAPDH, HPRT1, YWHAZ, and TBP) in the porcine ovaries under different estrus phase (follicular and luteal phase) and cystic condition, using stable RG-finding programs (geNorm, Normfinder, and BestKeeper). The significant (p < 0.01) differences in Ct values of RGs in the porcine ovaries under different conditions were identified. In assessing the stability of RGs, three programs comprehensively agreed that TBP and YWHAZ were suitable RGs to study porcine ovaries under different conditions but ACTB and GAPDH were inappropriate RGs in this experimental condition. We hope that these results contribute to plan the experiment design in the field of reproductive physiology in pigs as reference data.

협력형 참고봉사 서비스 비교 연구 - 한국과 일본을 중심으로 - (A Comparative Study on Cooperative Reference Service: Centering on Korea and Japan)

  • 조재인
    • 한국문헌정보학회지
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    • 제46권1호
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    • pp.119-139
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    • 2012
  • 이용자의 무관심, 대체할 수 있는 인터넷 컨텐츠의 급증으로 국내 협력형 참고봉사 서비스는 정체 상태에 빠져 있다. 특히 이용자가 스스로 탐색하여 획득 가능한 인터넷 컨텐츠의 급증은 전통적 질의-응답 방식의 참고봉사 서비스의 기능과 본질에 대한 재검토와 더불어, 신뢰할 수 있는 지식베이스의 구축이라는 참고봉사의 간접서비스 영역에 대한 중요성을 새롭게 조명하고 있다. 본 연구는 간접서비스 위주의 협력을 추구하는 있는 일본 도서관들의 협력형 참고봉사 서비스를 심층 조사하고 이를 비교 분석하여, 한국의 협력형 참고봉사 서비스의 새로운 도약 가능성을 조망해 본다.

Measurement of Proton Beam Dose-Averaged Linear Energy Transfer Using a Radiochromic Film

  • Seohyeon An;Sang-il Pak;Seonghoon Jeong;Soonki Min;Tae Jeong Kim;Dongho Shin;Youngkyung Lim;Jong Hwi Jeong;Haksoo Kim;Se Byeong Lee
    • 한국의학물리학회지:의학물리
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    • 제33권4호
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    • pp.80-87
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    • 2022
  • Purpose: Proton therapy has different relative biological effectiveness (RBE) compared with X-ray treatment, which is the standard in radiation therapy, and the fixed RBE value of 1.1 is widely used. However, RBE depends on a charged particle's linear energy transfer (LET); therefore, measuring LET is important. We have developed a LET measurement method using the inefficiency characteristic of an EBT3 film on a proton beam's Bragg peak (BP) region. Methods: A Gafchromic EBT3 film was used to measure the proton beam LET. It measured the dose at a 10-cm pristine BP proton beam in water to determine the quenching factor of the EBT3 film as a reference beam condition. Monte Carlo (MC) calculations of dose-averaged LET (LETd) were used to determine the quenching factor and validation. The dose-averaged LETs at the 12-, 16-, and 20-cm pristine BP proton beam in water were calculated with the quenching factor. Results: Using the passive scattering proton beam nozzle of the National Cancer Center in Korea, the LETd was measured for each beam range. The quenching factor was determined to be 26.15 with 0.3% uncertainty under the reference beam condition. The dose-averaged LETs were measured for each test beam condition. Conclusions: We developed a method for measuring the proton beam LET using an EBT3 film. This study showed that the magnitude of the quenching effect can be estimated using only one beam range, and the quenching factor determined under the reference condition can be applied to any therapeutic proton beam range.

도시에 거주하는 여자노인의 영양상태와 건강상태에 관한 조사연구 (A Study on the Nutritional Status and Health Condition of Elderly Women Living in Urban Community)

  • 김혜경
    • Journal of Nutrition and Health
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    • 제22권3호
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    • pp.175-184
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    • 1989
  • This survey was carried out to obtain the information concerning nutritional status, including factors of food intake and health condition of elderly women living in Ulsan city. Main results were as follows. 1) Dietary intake data showed the average daily intake of energy, protein, vitamin A, riboflavin and ascorbic acid were lower than Korean recommended dietary allowances (RDA). According to the percentage of RDA, Vitamin A was the nutrient found to be least sufficient. 2) Among the various influencial factors, pocket money, meal-mate, skip meal and health concern influenced on nutrient intake considerably. A meal condition score based on influencial factors, showed significant correlations with all nutrient intake(P<0.001). A health score based n the subjects complains about health condition, showed significant correlations with intake of energy, carbohydrate, thiamin, and niacin(P<0.05). 3) Average hight was below Korea reference, while average body weight of subjects was close to Korean reference. Average body mass index was 23.0. 4) The average systolic and diastolic blood pressure was 140mmHg/88mmHg, the proportion of hypertension was 32.4 percent. The average hemglobin concentration was 12.7g/dl, only 6.7 percent of subjects were anemic. Serum cholesterol concentration was normal range(140~220mg/dl) in all subjects.

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Eletromyographic Activities of Trunk and Lower Extremity Muscles During Bridging Exercise in Whole Body Vibration and Swiss Ball Condition in Elderly Women

  • Kim, Tack-Hoon;Lee, Kang-Seong
    • 한국전문물리치료학회지
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    • 제17권4호
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    • pp.26-34
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    • 2010
  • The purpose of this study was to compare the trunk and lower extremity muscle activity induced by six different conditions floor, intensity 0, 1, 3, 5 of whole body vibration (WBV), and Swiss ball during bridging exercise. Surface electromyography (EMG) was used to measure trunk and lower extremity muscles activity. Ten elderly women were recruited from Hong-sung Senior Citizen Welfare Center. The collected EMG data were normalized using reference contraction (during floor bridging) and expressed as a percentage of reference voluntary contraction (%RVC). To analyze the differences in EMG data, the repeated one-way analysis of variance was used. A Bonferroni's correction was used for multiple comparisons. The study showed that EMG activity of the rectus abdominis, external oblique, internal oblique, erector spinae and rectus abdominis muscles were not significantly different between six different conditions of during bridging exercise (p>.05). However, there were significantly increased EMG activity of the rectus femoris (p=.034) in the WBV intensity 0, 1, 3, and 5 conditions compared with the floor bridging condition. EMG activity of the medial gastrocnemius were significantly increased in the WBV intensity 0, 1, 3, 5 and Swiss ball conditions compared with the floor bridging condition. Future studies are required the dynamic instability condition such as one leg lifting in bridging.

예혼합기의 열적성층화가 PRF연료의 예혼합압축자기착화에 미치는 영향 (Research about Thermal Stratification Effect on HCCI Combustion Fueled with Primary Reference Fuel)

  • 임옥택
    • 한국자동차공학회논문집
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    • 제16권5호
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    • pp.157-163
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    • 2008
  • The HCCI combustion mode poses its own set of narrow engine operating by knocking. In order to solve this, inhomogeneity method of mixture and temperature is suggested. The purpose of this research is to get fundamental knowledge about the effect of thermal stratification on HCCI combustion of PRF -Air mixture. The temperature stratification is made by buoyancy effect in combustion chamber of RCM. The analysis items are pressure, temperature of in-cylinder gas and combustion duration. In addition, the structure of flames using the two dimensional chemiluminescence's images by a framing camera are analyzed. Under stratification, the LTR starting time and the HTR starting time are advanced than that of homogeneous. Further, the LTR period of homogeneous conditions became shorter than that of the stratified conditions. With the case of homogeneous condition, the luminosity duration becomes shorter than the case of stratified condition. Additionally, under stratified condition, the brightest luminosity intensity is delayed longer than at homogeneous condition.

PV모듈 발전성능 비교시험과 계측편차 요인 분석 (Analysis of Comparison Test and Measurement Error Factor for I - V Performance of Photovoltaic Module)

  • 강기환;김경수;유권종;안형근;한득영
    • 한국태양에너지학회 논문집
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    • 제29권2호
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    • pp.70-75
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    • 2009
  • In this experiment, we did sampling 6 kinds of photovoltaic modules and analyzed the discrepancy of measurement results between l laboratory and 4 PV makers to have performance repeatability at Standard Test Condition(STC) condition. From the KIER's results, Korea's standard test laboratory, other laboratory showed -10% measurement variation. The causes came from correction of reference cell, test condition and the state of skill. Form the comparison test, we analyzed the problems. But three PV maker reduced measurement variation, other one PV maker and one test laboratory didn't improve the problems of correction of reference cell, test condition and the state of skill. Also, High Efficiency Module had a big discrepancy of -10.0$\sim$-6.2% among 3 laboratories which have a less than 10msec light pulse duration time. This made low spectrum response speed so the Fill Factor decreased maximum output power under 10msec light pulse duration time

Model Reference Adaptive Control of a Flexible Structure

  • Yang, Kyung-Jinn;Hong, Keum-Shik;Rhee, Eun-Jun;Yoo, Wan-Suk
    • Journal of Mechanical Science and Technology
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    • 제15권10호
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    • pp.1356-1368
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    • 2001
  • In this paper, the model reference adaptive control (MRAC) of a flexible structure is investigated. Any mechanically flexible structure is inherently distributed parameter in nature, so that its dynamics are described by a partial, rather than ordinary, differential equation. The MRAC problem is formulated as an initial value problem of coupled partial and ordinary differential equations in weak form. The well-posedness of the initial value problem is proved. The control law is derived by using the Lyapunov redesign method on an infinite dimensional filbert space. Uniform asymptotic stability of the closed loop system is established, and asymptotic tracking, i. e., convergence of the state-error to zero, is obtained. With an additional persistence of excitation condition for the reference model, parameter-error convergence to zero is also shown. Numerical simulations are provided.

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HTML 문서의 무결성 유지 시스템의 설계 및 구현 (The Design and Implementation of HTML Document Integrity Management System)

  • 조이기;이영운;황인문;양수영;김원중
    • 한국정보통신학회:학술대회논문집
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    • 한국해양정보통신학회 2002년도 춘계종합학술대회
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    • pp.380-383
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    • 2002
  • KLDP(리눅스 한글 문서 프로젝트, http://kldp.org)와 같이 많은 분량의 HTML 문서들로 이루어진 매뉴얼 사이트를 관리하는데 가장 어려운 점은 현수 참조(dangling reference), 오문 참조 (inaccurate content reference)와 같은 깨진 링크(broken link)를 관리하는 것이다. 본 논문에서는 웹사이트의 HTML 문서들 사이에 존재하는 관계성(Relationship)과 제약 조건(Constraints)을 정의하여, HTML 문서의 삽입, 삭제, 변경이 발생할 경우 사용자에게 통지하거나, 트리거를 수행하여 HTML 문서들의 무결성(Integrity)을 유지할 수 있도록 하는 HIMS(HTML Document Integrity Management System)시스템을 설계 및 구현하였다.

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비전 기반의 FPC용 자동 펀칭시스템 (Automatic Punching System for FPC using Machine Vision)

  • 이영춘;이성철
    • 한국정밀공학회지
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    • 제22권12호
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    • pp.77-86
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    • 2005
  • This paper is about the development of Automatic FPC(flexible printed circuit) punching instrument for the improvement of working condition and cost saving. FPC is used to detect the contact position of keyboard and button like a cellular phone. Depending on the quality of the printed ink and position of reference punching point to the FPC, the resistance and current are varied to the malfunctioning values. The size of reference punching point is 2mm and the above. Because the punching operation is done manually, The punching accuracy is varied with operator's condition. Recently, The punching accuracy has deteriorated severely to the 2mm punching reference hall so that assembly of the K/B has hardly done. To improve this manual punching operation to the FPC, automatic FPC punching system is introduced. Precise mechanical parts like a 5-step stepping motor and ball screw mechanism are designed and tested and low cost PC camera is used fur the sake of cost down instead of using high quality vision systems for the factory automation. Test algorithms and programs showed good results to the designed automatic punching system and led to the increasement of productivity and huge cost down to law material like FPC by avoiding bad quality.