• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.2
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• pp.125-133
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• 2008

To investigate the effect of repeated food deprivation and refeeding on the hyperphagia, compensatory growth, feed efficiency, body composition, hepatosomatic index (HSI), and survival rate of the juvenile olive flounder Paralichthys olivaceus, an experiment was conducted for 90 days. Feeding treatments consisted of the following five regimes for 90 days from August to October, 2005: C: daily satiation feeding (control), S1: 1-day satiation feeding after 1-day food deprivation, S2: 1-day satiation feeding after 2-day food deprivation, S3: 1-day satiation feeding after 3-day food deprivation and S4: 1-day satiation feeding after 4-day food deprivation, respectively. Although the monthly feed intake (MFI) of the control was significantly higher than that of all of the starved groups, the daily feed intake (DFI) was more higher in S1, S2, and S3 than that in the control as a result of hyperphagia after starvation. While the feed efficiency in the summer (to day 30) decreased in all of the starved groups with prolongation of the starvation period, the feed efficiency in the autumn (to day 90) was increased with prolongation of the starvation period. The whole body proximate composition and HSI were also affected by starvation. The crude protein, lipid, and HSI decreased with prolongation of the starvation period, whereas the crude ash and moisture increased. The growth rate and condition factor also decreased in proportion to the starvation period. The survival rate was highest in the control and was the lowest in S4. In this study, although hyperphagia occurred in the deprived groups, we knew that the compensatory growth did not always occur.

• Journal of Life Science
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• v.33 no.10
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• pp.828-834
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• 2023

The cDNA that encodes transmembrane protein 258 (Tmem258) was cloned from Gryllus bimaculatus and named GbTmem258. This protein comprises 80 amino acids, has no N-glycosylation site, and contains five potential phosphorylation sites at two serines, two threonines, and one tyrosine. The predicted molecular mass of GbTmem258 is 9.06 kDa, and its theoretical isoelectric point is 5.5. The tertiary structure of GbTmem258 was predicted using the available secondary structure information, which suggests the presence of alpha helices (52.5%), random coils (22.5%), extended strands (16.25%), and beta turns (8.75%). Homology analysis revealed that GbTmem258 exhibits high similarity at the amino-acid level to Tmem258 found in other species. The effect of starvation and refeeding on GbTmem258 mRNA expression was also examined in this study. It was found that GbTmem258 mRNA expression in the hindgut progressively increased throughout the starvation period, peaking at almost 1.5 times the control level after six days of starvation. However, refeeding for one to two days after the six-day starvation period restored GbTmem258 mRNA expression to the control level. In fat body, GbTmem258 mRNA expression was almost 3-fold higher during starvation compared to the control level. Refeeding for one to two days after the six-day fast resulted in a decline in the expression to about a 2.5-fold increase over the control level. Throughout the starving and refeeding periods, no other tissues showed any discernible alterations in GbTmem258 mRNA expression.

• The Journal of Pediatrics of Korean Medicine
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• v.19 no.2
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• pp.1-11
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• 2005

Objectives : The purpose of this study is to investigate the effect of fasting therapy on the changes of body composition in obese adolescents. Methods : We examined the body composition of obese adolescents three times; before fasting, on the second refeeding day, two or four weeks later. Results : Body weight, body mass index(BMI), basal metabolic rate(BMR) and lean body mass (LBM) were decreased during the fasting, whereas percent-fat was increased. After fasting, patients have the dietary period. During this period BMR and LBM were increased and the decrease of percent-fat was continued. Conclusions : It seems that fasting therapy with keeping up well-controlled dietary period and receiving oriental medical management can be useful to decrease percent-fat in obese patients.

• Korean Journal of Microbiology
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• v.26 no.1
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• pp.44-51
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• 1988

The intracellular free radicals produced at different stages of cell cycle of Saccharomyces cerevisiae ATCC 24858 were investigated by means of electron spin resonance(ESR) spectroscopy. The synchronized cells by repeated starvation and refeeding revealed different ESR spectral pattern compared to that of asynchronized cells. Each spectrum centered at g=2.005, which indicates free radicals. The relative spin concentration was maximat at the end of DNA increase. The variation of the relative spin concentration at each distinct stage of the cell cycle was evaluated in relation to ascorbate concentration, L-galactonolactone oxidase activity, and ascorbate oxidase activity. The highest activities of L-galactonolactone oxidase and ascorbate oxidase were detected just before and at the maximum of relative spin concentration, respectively. And ascorbate concentration fluctuated through each stage of cell cycle with the changes of relative spin concentration, L-galactonolactone oxidase activity, and ascorbate oxidase activity. Thus it is suggested that intracellular free radicals should be related to cell cycle, interacted with ascorbate, and may play an important role in the cell cycle of Saccharomyces cerevisiae.

• Applied Biological Chemistry
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• v.23 no.1
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• pp.23-30
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• 1980

Labelling of chromatin proteins with 32P was observed after incubating isolated liver nuclei with $[{\gamma}-32P]$ ATP for 5 minutes at $37^{\circ}C$. The pattern of labelling with 32P was examined on SDS polyacrylamide gel electrophoresis with nuclei from rats maintained in a starvation state for 48 hours, following refeeding for 12 hours; and from fed streptozotocin-diabetic rats with insulin injection 6 hours before sacrifice. With 48h starved rat liver nuclei the level of phosphorylation for 0.14M NaCl soluble proteins was decreased in the molecular weights between 41,000 and 200,000 daltons relative to normal controls. Refeeding the starved rats reversed the change of phosphorylation pattern over 12 hour The level of phosphorylation for five phenol soluble non-histone proteins with molecular weights above 59,000 daltons was somewhat decreased with 48h starved rat liver nuclei as compared with that of normal controls. Starvation also decreased the phosphorylation level of major histones in relation to normal controls. The experiment with insulin injection into fed streptozotocin-diabetic rats showed the tendency to increase phosphorylation of 0.14M NaCl soluble proteins (130,000 dalton protein) and phenol soluble non-histone proteins (155,000 dalton protein). The phosphorylation level of histones appeared to be invariant under the experimental conditoins employed here. These results suggest the possibility that the phosphorylation and dephosphorylation of 0.14M NaCl soluble proteins and $H_1$ histone precede those of other chromatin associated nuclear proteins, It is of interest to find that insulin signal was correlated to phosphorylation of nuclear proteins while glucagon signet dephosphorylated nuclear proteins.

• Journal of Veterinary Clinics
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• v.28 no.6
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• pp.581-585
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• 2011

Either the fasting during natural molting or the starvation in induced molting would be a severe metabolic stress to laying hens. The metabolic stress during starvation and subsequent refeeding syndrome could lead to unbalance of mineral homeostasis, including $Mg^{2+}$, $K^+$ and P required by ATP synthesis. Since $Mg^{2+}$ is a fundamental ion for normal metabolic processes and stress may not only increase in demands of $Mg^{2+}$ but also produce consequence of $Mg^{2+}$ deficiency, we investigated the changes of blood ionized and total ions related to starvation during molting in laying hens. We founded the significant decrease in blood $Mg^{2+}$ and $K^+$ accompanied by the changes of biochemical parameters relating to increased metabolic stress after molting. These results suggested that appropriate $Mg^{2+}$ and $K^+$ supplements to laying hens could have beneficial effects during molting and subsequent refeeding that could produce a severe hypomagnesemia and hypokalcemia.

• Journal of Korean Medicine for Obesity Research
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• v.16 no.1
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• pp.50-63
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• 2016

Objectives: The aim of this study is to analyze methods and outcomes about fasting for obesity in korea medicine research. Methods: We searched the studies with key words of 'obesity' and 'fasting or starvation' in Korean database (Korean traditional knowledge portal, OASIS, KISS, KMBASE, DBPIA, RISS). Studies were analyzed for contents, evaluation methods and adverse effects. Results: Twenty-one studies were reviewed. Studies were published in 1983~2015. 1. Studies can be classified into three types: comparative study on before and after, comparative study between groups, case study on other disease occurred during the fasting therapy period. 2. The most common step of fasting were 3 steps: reducing food intakes period (3~5 days), fasting period (7~10 days) and refeeding period (3~5 days) and additional period-diet period (28 days). 3. The most common evaluation methods were body weight (BW), body fat mass (BFM), muscle weight (MW), fat free mass, body mass index, body fat percentage, etc. 4. BW, BFM, MW were reduced largely in fasting period. 5. The most common adverse effects were fatigue, nausea, headache, vomiting, insomnia, etc. Conclusions: As a result of the review papers, fasting therapy have positive effects for obesity. To verify the effectiveness of the fasting, more studies are needed such as randomized controlled trial.

• Korean Journal of Veterinary Research
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• v.30 no.1
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• pp.15-27
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• 1990

Effects of oral administration of electrolyte solutions were studied in experimentally dehydrated adult sheep. By the latin square method five ruminal fistulated sheep were examined and dehydrated by deprivation of feed and water for 72 hours. Tap water, physiological saline, 0.45% NaCl+120 mM/L glucose and 0.9% NaCl+1% propylene glycol solution were orally administrated after dehydration, respectively. Rehydration effect and modification of the rumen function were compared. 1. After 72 hours of deprivation of feed and water, sheep were hypertonic dehydrated and blood acid-base parameters were not significantly changed. And there was marked increase in ruminal pH and decrease in ruminal total volatile fatty acid(VFA) concentration. 2. After the fluids administration the changes in blood acid-base parameters were not significant in all groups. 3. Although glucose fermentation in the rumen was observed, 0.45% NaCl+120 mM/L glucose was more effective in rehydration than physiological saline and tap water. But it was difficult to know the rehydration effect of 0.9% NaCl+1% propylene glycol solution exactly because of excessive increase in plasma osmolality. 4. After refeeding, total concentration and proportions of ruminal volatile fatty acid(VFA) were not significantly different among groups and recovered to normal concentration but not in proportions after 2 days in all groups. 5. In vitro cultured ruminal protozoa were susceptible to the decrease of the pH and osmolality.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.3
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• pp.401-410
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• 1993

Day-old male and female broilers obtained from commercial strain (Hubbard) were used in this study. At 7 days of age, all chicks were weighed individually (female 0.11 kg and male 0.12 kg in average) and divided into 5 group of 45 birds each group with no replicate in each treatment group as follows: 1. fed ad libitum; 2. fed 75% ad libitum; 3. fed 65% ad libitum; 4. fed 55% ad libitum; 5. fed 45% ad libitum. Chicks were restricted for 10 days (d 7 through 17) of a 56-day trial. Chicks were fed a commercial starter diet (crude protein (CP) 23.8% and metabolizable energy (ME) 3,070 kcal/kg) for 21 days, and commercial finisher diet (CP 20% and ME 3,160 kcal/kg) from 22 to 56 days of age. When chicks were feed-restricted at an early age, compensatory growth did not immediately occur following refeeding. Body weights of restricted chicks were not equal to the ad libitum chicks before 49 days of age. At 56 dyas of age, body weights of restricted chicks were heavier (p<0.01). Abdominal fat values of female chicks fed ad libitum was not significantly different from those fed 45% to 75% ad libitum. Carcass fat values of restricted female chicks were lower than those of control chicks when female chicks were fed either 55% or 45% ad libitum. Furthermore, feed efficiencies of restricted chicks were better. The response of chicks to early feed restriction might depend on the degree of feed restriction, and sex. Factors contributing carcass fat included energy loss, fat protein ratio of carcass, fatty acid synthesis in the liver and triglyceride content of the liver. The heavier body weights in the restricted chicks might be correlated with the lower growth rate during period of feed restriction.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.9
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• pp.1300-1308
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• 2012

The 5'-adenosine monophosphate-activated protein kinase (AMPK) is a key part of a kinase-signaling cascade that acts to maintain energy homeostasis. The objective of this experiment was to investigate the possible effects of fasting and refeeding on the gene expression of hypothalamic AMPK, some appetitive regulating peptides and lipid metabolism related enzymes. Seven-day-old male broiler (Arbor Acres) chicks were allocated into three equal treatments: fed ad libitum (control); fasted for 24 h; fasted for 24 h and then refed for 24 h. Compared with the control, the hypothalamic gene expression of $AMPK{\alpha}2$, $AMPK{\beta}1$, $AMPK{\beta}2$, $AMPK{\gamma}1$, Ste20-related adaptor protein ${\beta}$ ($STRAD{\beta}$), mouse protein $25{\alpha}$ ($MO25{\alpha}$) and agouti-related peptide (AgRP) were increased after fasting for 24 h. No significant difference among treatments was observed in mRNA levels of $AMPK{\alpha}1$, $AMPK{\gamma}2$, LKB1 and neuropeptide Y (NPY). However, the expression of $MO25{\beta}$, pro-opiomelanocortin (POMC), corticotropin-releasing hormone (CRH), ghrelin, fatty acid synthase (FAS), acetyl-CoA carboxylase ${\alpha}$ ($ACC{\alpha}$), carnitine palmitoyltransferase 1 (CPT-1) and sterol regulatory element binding protein-1 (SREBP-1) were significantly decreased. The present results indicated that 24 h fasting altered gene expression of AMPK subunits, appetite regulation peptides and lipometabolism related factors in chick's hypothalamus; the hypothalamic FAS signaling pathway might be involved in the AMPK regulated energy homeostasis and/or appetite regulation in poultry.