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Effects of Various Plant Growth Promoting Rhizobacteria on the Early Growth of Red Pepper Seedlings, Capsicum annuum L. cv. Nockkwang (數種의 植物生長促進 根圈細菌이 '녹광' 고추 幼苗의 初期生長에 미치는 影響)

  • Cho, Ja-Yong;Chung, Soon-Ju
    • Korean Journal of Organic Agriculture
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    • v.7 no.1
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    • pp.137-146
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    • 1998
  • Various rhizobacteria was isolated, and was bacterialized into the substrates to clarify the plant growth promoting effects of rhizobacteria on the early growth of red peper seedlings. Total 125 bacterial isolates were primarily isolated and purified from the soils in greenhouse. And four strains were finally screened, based on the antifungal activities against Fusarium sp., Pythium sp. and Rhizoctonia sp. of red pepper plants. The strongest antifungal strain RB 109 has a antagonistic activity against Fusarium sp., Pythium sp. and Rhizoctonia sp. in terms of 66.0%, 65.0% and 66.1%. Early growth of red pepper seedlings was promoted, when cultured solution of rhizobacteria RB 109 was bacterialized into the substrates. Antifungal rhizobacteria RB 109 was identified as Pseudomonas sp. related strains, which has a similarity of 82% to the Pseudomonas sp.

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Red blood cell distribution width is useful in discriminating adult onset Still's disease and sepsis within 24 hours after hospitalization

  • Park, Hee-Jin;Song, Jungsik;Park, Yong-Beom;Lee, Soo-Kon;Lee, Sang-Won
    • The Korean journal of internal medicine
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    • v.33 no.6
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    • pp.1234-1240
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    • 2018
  • Background/Aims: Red blood cell distribution width (RDW) is a value representing the heterogeneity in the size of red blood cell, and it is usually used in distinguishing types of anaemia. Recently, it was reported that it could reflect the burden of inflammation in diverse diseases and their prognosis. Hence, in this study, we investigated whether RDW may contribute to discriminating adult onset Still's disease (AOSD) from sepsis in serious febrile patients within 24 hours after hospitalization. Methods: We reviewed the medical records and enrolled 21 AOSD patients, 27 sepsis patients and 30 matched healthy controls. We collected at least two laboratory results of variables including RDW within 24 hours after hospitalization, and we calculated their mean values. Results: Sepsis patients showed the significantly increased median white blood cell count, compared to AOSD patients ($14,390.0/mm^3$ vs. $12,390.0/mm^3$, p = 0.010). The median RDW in sepsis patients was higher than that in AOSD patients (15.0% vs. 13.3%, p = 0.001), and furthermore, the median RDW in both patient-groups was significantly higher than that in healthy controls. In contrast, the median ferritin level in sepsis patients was lower than that in AOSD patients (544.0 mg/dL vs. 3,756.6 mg/dL, p = 0.001). In multivariate analysis, RDW ${\geq}14.8%$ (odds ratio, 17.549) and ferritin < 2,251.0 mg/dL (odds ratio, 32.414) independently suggested sepsis more than AOSD in patients initially presenting with fever requiring hospitalization. Conclusions: RDW might be a rapid and helpful marker for a differential diagnosis between AOSD from sepsis at an early phase.

Multicomponent assessment and ginsenoside conversions of Panax quinquefolium L. roots before and after steaming by HPLC-MSn

  • Huang, Xin;Liu, Yan;Zhang, Yong;Li, Shuai-Ping;Yue, Hao;Chen, Chang-Bao;Liu, Shu-Ying
    • Journal of Ginseng Research
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    • v.43 no.1
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    • pp.27-37
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    • 2019
  • Background: The structural conversions in ginsenosides induced by steaming or heating or acidic condition could improve red ginseng bioactivities significantly. In this paper, the chemical transformations of red American ginseng from fresh Panax quinquefolium L. under steaming were investigated, and the possible mechanisms were discussed. Methods: A method with reversed-phase high-performance liquid chromatography coupled with linear ion trap mass spectrometry ($HPLC-MS^n$)-equipped electrospray ionization ion source was developed for structural analysis and quantitation of ginsenosides in dried and red American ginseng. Results: In total, 59 ginsenosides of protopanaxadiol, protopanaxatriol, oleanane, and ocotillol types were identified in American ginseng before and after steaming process by matching the molecular weight and/or comparing $MS^n$ fragmentation with that of standards and/or known published compounds, and some of them were determined to be disappeared or newly generated under different steaming time and temperature. The specific fragments of each aglycone-type ginsenosides were determined as well as aglycone hydrated and dehydrated ones. The mechanisms were deduced as hydrolysis, hydration, dehydration, and isomerization of neutral and acidic ginsenosides. Furthermore, the relative peak areas of detected compounds were calculated based on peak areas ratio. Conclusion: The multicomponent assessment of American ginseng was conducted by $HPLC-MS^n$. The result is expected to provide possibility for holistic evaluation of the processing procedures of red American ginseng and a scientific basis for the usage of American ginseng in prescription.

Studies on the Osmophilic Red Color Yeast (1) Isolation and Identification of Sporobolomyces sp. (내염성 적색효모에 관한 연구 (제 1 보) Sporobolomyces 속의 분리 및 동정)

  • Lee, Taik-Soo;Yu, Ju-Hyun;Chu, Yong-Ha;O, Mahn-Jin
    • Korean Journal of Food Science and Technology
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    • v.2 no.2
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    • pp.26-29
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    • 1970
  • Four strains of Sporobolomyces genera were isolated from soy sauce mashes at the fermentating and aging periods and identified. Their salt-resistance and color formation were examined in order to improve the color density and nutrition of red pepper paste preparations. The results obtained were as follows; a) The strain L1, L2, L3 and L4 isolated were identified as Sporobolomyces gracilis, Sporobolomyces roseus, Sporobolomyes gracilis and Sporobolomyces roseus respectively. b) The yeasts isolated were grown on the media containing $0{\sim}10$ percent of sodium chloride but their growth were almost restrained on the media containing $15{\sim}18$ per cent of sodium chloride. And the strain L2 and L3 were more salt-resistant as compared with L1 and L4. c) The strain L3 were the best among the 4 strains of yeasts comparison with their salt-resistance, color and flavour formation.

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Bioelectrochemical Mn(II) Leaching from Manganese Ore by Lactococcus lactis SK071115

  • Jeon, Bo-Young;Park, Doo-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.21 no.2
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    • pp.154-161
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    • 2011
  • L. lactis sk071115 has been shown to grow more actively and generate lower levels of lactate in glucose-defined medium with nitrate than in medium with Mn(IV). By adding Mn(IV) to a L. lactis culture, lactate production was relatively reduced in combination with Mn(II) production, but cell mass production levels did not increase. Both cell-free extract and intact L. lactis cells reacted electrochemically with Mn(IV) but did not react with Mn(II) upon cyclic voltammetry using neutral red (NR) as an electron mediator. A modified graphite felt cathode with NR (NR-cathode) was employed to induce electrochemical reducing equivalence for bacterial metabolism. Cell-free L. lactis extract catalyzed the reduction of Mn(IV) to Mn(II) under both control and electrochemical reduction conditions; however, the levels of Mn(II) generated under electrochemical reduction conditions were approximately 4 times those generated under control conditions. The levels of Mn(II) generated by the catalysis of L. lactis immobilized in the NR-cathode (L-NR-cathode) under electrochemical reduction conditions were more than 4 times that generated under control conditions. Mn(II) production levels were increased by approximately 2.5 and 4.5 times by the addition of citrate to the reactant under control and electrochemical reduction conditions, respectively. The cumulative Mn(II) produced from manganese ore by catalysis of the L-NR-cathode for 30 days reached levels of approximately 3,800 and 16,000 mg/l under control and electrochemical reduction conditions, respectively. In conclusion, the electrochemical reduction reaction generated by the NR-cathode activated the biochemical reduction of Mn(IV) to Mn(II) by L. lactis.

Correlation between Corrosion Rate and Red Water on Application of Corrosion Inhibitor in Drinking Water Distribution System (배급수 계통에서 부식억제제 적용에 따른 부식과 적수와의 상관관계)

  • Woo, Dal-Sik;Ku, Sung-Eun;Lee, Byung-Doo;Kim, Ju-Hwan;Moon, Kwang-Soon
    • Journal of Korean Society of Water and Wastewater
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    • v.19 no.1
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    • pp.68-77
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    • 2005
  • This study was performed to evaluate the application of corrosion inhibitor and to examine the correlation between corrosion rate and red water in a series of batch tests and a test using auto corrosion monitoring system at A water treatment plant in Gyeonggido. The corrosion study in the auto corrosion monitoring system indicated that Fe concentration decreased by 30~50% and corrosion rate also reduced remarkably with corrosion inhibitor at $1.8mg\;PO_4/L$. After addition of corrosion inhibitor, it was indicated the effective adsorption of the inhibitor on the surface of the pipe line forming a protective film. The corrosion rate increases with the increase in Fe concentration. With $1.8mg\;PO_4/L$ of corrosion inhibitor, the corrosion rate decreased remarkably. Fe concentration had correlation to not only red water problems but also the corrosion rate that actually dissolved into the water, primarily due to the deposition of oxidized iron or other compounds as a scale, which serves as a large reservoir of corrosion by-product. Therefore, corrosion rate can be estimated by Fe concentration. For these reasons, an effective corrosion inhibitor is also an effective red water control reagent. The effect of the corrosion inhibition can last for some time even the application the corrosion inhibitor is discontinued. For the cost effective and efficient corrosion control, the concentration and timing of corrosion inhibitor addition must be determined properly.

Effect of LEDs (Light Emitting Diodes) Irradiation on Growth and Mineral Absorption of Lettuce (Lactuca sativa L. 'Lollo Rosa') (LED 광원이 상추의 생육 및 무기물 흡수에 미치는 영향)

  • Shin, Yong Seub;Lee, Mun Jung;Lee, Eun Sook;Ahn, Joon Hyung;Lim, Jae Ha;Kim, Ha Joong;Park, Hoo Won;Um, Young Ghul;Park, So Deuk;Chai, Jang Heui
    • Journal of Bio-Environment Control
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    • v.21 no.3
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    • pp.180-185
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    • 2012
  • The objective of this study was carried out to elucidate the effect of LEDs (light emitting diodes) irradiation in relation to early growth and inorganic elements in leaf lettuce (Lactuca sativa L. 'Rollo Rosa'). In morphological changes of leaves, shoot elongation and hypocotyl length showed poor growth in red light irradiation, while the red + blue light irradiation induced shorter plant height and much greater leaf numbers resulting in increased fresh weight. In change of the Hunter's color and SPAD values, lettuce seedlings grown under in red + blue and fluorescent light irradiation had a higher $a^*$ value, otherwise SPAD values were not changed in these light irradiations. Interestingly, relative chlorophyll contents showed 1.8 times increased redness in the treatment of red + blue light irradiation. Inorganic element (N, Ca, Mg, Mn, and Fe) and ascorbic acid contents were increased in lettuce plants grown under LEDs light irradiation compared to those of lettuce grown under the fluorescent light which showed higher P and Mn contents. In conclusion, it is considered that red + blue light irradiation which stimulates growth and higher nutrient uptake in leaf lettuce could be employed in containers equipped with LEDs.

Isolation of Immunomodulatory Antitumor Active Polysaccharide (RGAP) from Red Ginseng By-Product and Its Physico-chemical Properties (홍삼추출잔사로부터 항암면역조절 활성을 보여주는 홍삼산성다당체(RGAP)의 분리 및 이화학적 특성)

  • Kwak, Yi-Seong;Shin, Han-Jae;Song, Yong-Bum;Park, Jong-Dae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.5
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    • pp.752-757
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    • 2003
  • This study was carried out in order to develop the method for isolation of red ginseng acidic polysaccharide (RGAP) haying immunomodulating antitumor activity from red ginseng by-product. The red ginseng by-product was obtained from red ginseng residues produced in processing of red ginseng ethanol extract. The yield of RGAP isolated by ultrafiltration was 20.9%. The active substance (GFP) was purified by DEAE-sepharose column chromatography RGAP induced nitric oxide (NO) exhibited tumoricidal activities against P8l5 (mastocytoma) tumor cells. Acid-hydrolyzed RGAP fragments were shown four to five spots. These sopts showed the same R$_{f}$ values with sugars designated as rhamnose, glucose, glactose and glucuronic acid. Some physico-chemical properties of RGAP were investigated. pH and dry reduction content at 105$^{\circ}C$ were 4.74 and 4.72%, respectively. Crude protein, ash and Pb contents were 3.30%, 4.74% and 2.30 ppm. These results suggest that we will be able to produce RGAP from red ginseng by-product by ultrafiltration in a large scale.e.

Effects of Dietary Supplementation of Red Ginseng Mare and Korean Mistletoe Powder on Performance and Meat Quality of Broiler Chicken (사료 내 홍삼박과 겨우살이 분말 첨가 급여가 육계의 생산성 및 계육의 품질에 미치는 영향)

  • Kim, Young-Jik
    • Korean Journal of Poultry Science
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    • v.41 no.3
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    • pp.197-204
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    • 2014
  • The objective of this study was to evaluate the effects of different levels of red ginseng mare or Korean mistletoe powder on growth performance and physicochemical properties of thigh meat in broiler. A total of 200 broiler chicks were divided into five groups were fed a basal diet (control), basal diet supplemented with 0.5% red ginseng mare (T1), red ginseng mare 1% added (T2), Korean mistletoe 0.5% added (T3), Korean mistletoe 0.5% added (T4) for five weeks. Growth performance and proximate composition of chicken thigh meat were not influenced by all treatments, except for mortality and crude fat, which was significantly lower in all treatments with red ginseng mare or Korean mistletoe powder compared to control. Dietary red ginseng mare and Korean mistletoe supplementation had no significantly effects on pH, WHC, shear force, CIE $L^*$, $b^*$ values, triglyceride and glucose but TBARS values total cholesterol, and LDL-cholesterol were decreased (P<0.05) by the addition red ginseng mare and Korean mistletoe powder. CIE $a^*$ values increased significantly with added red ginseng mare and Korean mistletoe powder relative to the control (P<0.05). It was concluded that the addition of red ginseng mare and Korean mistletoe powder revealed minor improvements in the lipid oxidative stability of chicken meat.

Interactions between common heterotrophic protists and the dinoflagellate Tripos furca: implication on the long duration of its red tides in the South Sea of Korea in 2020

  • Eom, Se Hee;Jeong, Hae Jin;Ok, Jin Hee;Park, Sang Ah;Kang, Hee Chang;You, Ji Hyun;Lee, Sung Yeon;Yoo, Yeong Du;Lim, An Suk;Lee, Moo Joon
    • ALGAE
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    • v.36 no.1
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    • pp.25-36
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    • 2021
  • The mixotrophic dinoflagellate Tripos furca causes red tides in the waters of many countries. To understand its population dynamics, mortality due to predation as well as growth rate should be assessed. Prior to the present study, the heterotrophic dinoflagellates Noctiluca scintillans, Polykrikos kofoidii, Protoperidinium steinii, and mixotrophic dinoflagellate Fragilidium subglobosum were known to ingest T. furca. However, if other common heterotrophic protists are able to feed on T. furca has not been tested. We explored interactions between T. furca and nine heterotrophic dinoflagellates and one naked ciliate. Furthermore, we investigated the abundance of T. furca and common heterotrophic protists in coastal-offshore waters off Yeosu, southern Korea, on Jul 31, 2020, during its red tide. Among the tested heterotrophic protists, the heterotrophic dinoflagellates Aduncodinium glandula, Luciella masanensis, and Pfiesteria piscicida were able to feed on T. furca. However, the heterotrophic dinoflagellates Gyrodiniellum shiwhaense, Gyrodinium dominans, Gyrodinium jinhaense, Gyrodinium moestrupii, Oblea rotunda, Oxyrrhis marina, and the naked ciliate Rimostrombidium sp. were unable to feed on it. However, T. furca did not support the growth of A. glandula, L. masanensis, or P. piscicida. Red tides dominated by T. furca prevailed in the South Sea of Korea from Jun 30 to Sep 5, 2020. The maximum abundance of heterotrophic dinoflagellates in the waters off Yeosu on Jul 31, 2020, was as low as 5.0 cells mL-1, and A. glandula, L. masanensis, and P. piscicida were not detected. Furthermore, the abundances of the known predators F. subglobosum, N. scintillans, P. kofoidii, and Protoperidinium spp. were very low or negligible. Therefore, no or low abundance of effective predators might be partially responsible for the long duration of the T. furca red tides in the South Sea of Korea in 2020.