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http://dx.doi.org/10.3746/jkfn.2003.32.5.752

Isolation of Immunomodulatory Antitumor Active Polysaccharide (RGAP) from Red Ginseng By-Product and Its Physico-chemical Properties  

Kwak, Yi-Seong (KT & G Central Research Institute)
Shin, Han-Jae (KT & G Central Research Institute)
Song, Yong-Bum (KT & G Central Research Institute)
Park, Jong-Dae (KT & G Central Research Institute)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.32, no.5, 2003 , pp. 752-757 More about this Journal
Abstract
This study was carried out in order to develop the method for isolation of red ginseng acidic polysaccharide (RGAP) haying immunomodulating antitumor activity from red ginseng by-product. The red ginseng by-product was obtained from red ginseng residues produced in processing of red ginseng ethanol extract. The yield of RGAP isolated by ultrafiltration was 20.9%. The active substance (GFP) was purified by DEAE-sepharose column chromatography RGAP induced nitric oxide (NO) exhibited tumoricidal activities against P8l5 (mastocytoma) tumor cells. Acid-hydrolyzed RGAP fragments were shown four to five spots. These sopts showed the same R$_{f}$ values with sugars designated as rhamnose, glucose, glactose and glucuronic acid. Some physico-chemical properties of RGAP were investigated. pH and dry reduction content at 105$^{\circ}C$ were 4.74 and 4.72%, respectively. Crude protein, ash and Pb contents were 3.30%, 4.74% and 2.30 ppm. These results suggest that we will be able to produce RGAP from red ginseng by-product by ultrafiltration in a large scale.e.
Keywords
Panax ginseng; red ginseng by-product; red ginseng acidic polysaccharide (RGAP); immunomodulatory antitumor activity;
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