• ALGAE
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• v.28 no.4
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• pp.297-305
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• 2013

There is increasing interest in the relationships between marine bacteria and red tide organisms. Some bacteria are known to kill red tide organisms, and may be responsible for accelerating the termination of red tides. Thus, certain algicidal bacteria have been proposed for the control of red tides. Meanwhile, many red tide organisms are known to feed on marine bacteria. The roles of marine bacteria and red tide organisms are therefore reversible. In Korean waters, the killing of red tide organisms by algicidal bacteria, and also the feeding of red tide organisms on marine bacteria have been extensively investigated. The findings of such studies may influence the conventional view of red tide dynamics, and also planktonic food webs. Here, we review the species and concentrations of algicidal bacteria that kill red tide organisms in Korean waters, as well as the ingestion rate and grazing impact of red tide organisms on marine bacteria. Furthermore, we offer an insight into the ecological roles of these 2 components in marine planktonic food webs.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.3
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• pp.248-255
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• 2010

This study was carried out to assess the feasibility of the electron beam irradiation as a mean of red tide control in coastal water. Prorocentrum minimum, Prorocentrum micans, Cochlodinium polykrikoides, Heterosigma akashiwo, Alexnadrium catenella were selected and cultured for experiments, and red tide occurring in Tongyeong(2007. 8. 15) was also tested under the same conditions. The irradiation dose were 1 kGy, 2 kGy, 4 kGy and 8 kGy. The result showed 50~65% extinction in red tide cells was observed right after irradiation dose of 1 kGy and 86~97% within 1 day after irradiation, compared with control. Chlorophyll-a concentration of red tide was reduced by 50~64% immediately and it was drastically reduced up to 86~97% 1 day after irradiation. When the culture was irradiated at 1 kGy, 28~47% of s-protein was released immediately, and 77~138% was released 1day after irradiation. 77~212% of s-carbohydrate was excreted after 1 day while 16~45% of s-carbohydrate was excreted immediately. A transmission electron microscope(TEM) observation for the irradiated red tide revealed that the cell was destroyed and intracellular biopolymeric substance was leached out from the damaged cell as a result of electron beam irradiation. These results imply that electron beam irradiation is enable to control red tide by flocculation with extracellular biopolymer. The paralytic shellfish poisoning(PSP) toxin contents produced by Alexandrium catenella was decreased 48% by 1 kGy of electron beam irradiation compared with the unirradiated cells. As a result, electron beam irradiation was effective for detoxication as well as destruction of red tide.

• Korean Journal of Remote Sensing
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• v.34 no.2_2
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• pp.377-391
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• 2018

In order to efficiently monitor red tide over a wide range, the need for red tide detection using remote sensing is increasing. However, the previous studies focus on the development of red tide detection algorithm for ocean colour sensor. In this study, we propose the use of multi-sensor to improve the inaccuracy for red tide detection and remote sensing data in coastal areas with high turbidity, which are pointed out as limitations of satellite-based red tide monitoring. The study area were selected based on the red tide information provided by National Institute of Fisheries Science, and spatial fusion and spectral-based fusion were attempted using GOCI image as ocean colour sensor and Landsat OLI image as terrestrial sensor. Through spatial fusion of the two images, both the red tide of the coastal area and the outer sea areas, where the quality of Landsat OLI image was low, which were impossible to observe in GOCI images, showed improved detection results. As a result of spectral-based fusion performed by feature-level and rawdata-level, there was no significant difference in red tide distribution patterns derived from the two methods. However, in the feature-level method, the red tide area tends to overestimated as spatial resolution of the image low. As a result of pixel segmentation by linear spectral unmixing method, the difference in the red tide area was found to increase as the number of pixels with low red tide ratio increased. For rawdata-level, Gram-Schmidt sharpening method estimated a somewhat larger area than PC spectral sharpening method, but no significant difference was observed. In this study, it is shown that coastal red tide with high turbidity as well as outer sea areas can be detected through spatial fusion of ocean colour and terrestrial sensor. Also, by presenting various spectral-based fusion methods, more accurate red tide area estimation method is suggested. It is expected that this result will provide more precise detection of red tide around the Korean peninsula and accurate red tide area information needed to determine countermeasure to effectively control red tide.

• Journal of Wetlands Research
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• v.13 no.2
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• pp.363-375
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• 2011

Recently, Water quiality of urban river largely have gotten better by virtue of sewer pipe laying and sewage treatment plants construction. or the various contaminants which is flowed in into river have generated underwater ecosystem disturbance and red tide by lack of sewage and waste water disposal facilities. With tidal river, taehwa river of ulsan metropolitan city has large river width and gradual stream bed gradient at the dry and storage period. Moreover, the flow is paralyzed due to the bridge pier protection work, consist of the mat foundation which is about 1.2km from two bridge and the contaminant is accumulated. it is caused by of the red tide generated from the several years or it activates. In this study, When flow area is largest by changing independent footing of bridge pier of two bridges and using RMA2 model, we hydraulically analyzed a variable breadth of velocity and discharge. Consequently, flow rate increased the maximum 103%, discharge was exposed to increase the maximum 61%. Directly this cannot extinguish the red tide but suppresses the red tide occurrence or can reduce. And it is determined to prevent the depositioning of the contaminant and can control fundamentally the red tide occurrence cause.

• Ocean and Polar Research
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• v.35 no.1
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• pp.15-26
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• 2013

The toxic, Harmful Algal Blooms (HABs) caused by the Cochlodinium polykrikoides have a serious impact on the coastal waters of Korea. In this study, the acoustic detection system was developed for rapid HABs detection, based on the acoustic backscattering properties of the C. polykrikoides. The developed system was mainly composed of a pulser-receiver board, a signal processor board, a control board, a network board, a power board, ultrasonic sensors (3.5 and 5.0 MHz), an environmental sensor, GPS, and a land-based control unit. To evaluate the performance of the system, a trail was done at a laboratory, and two in situ trials were conducted: (1) when there was no red tide, and (2) when there was red tide. In the laboratory evaluation, the system performed well in accordance with the number of C. polykrikoides in the received level. Second, under the condition when there was no red tide in the field, there was a good correlation between the acoustic data and sampling data. Finally, under the condition when there was red tide in the field, the system successfully worked at various densities in accordance with the number of C. polykrikoides, and the results corresponded with the sampling data and monitoring result of NFRDI (National Fisheries Research & Development Institute). From the laboratory and field evaluations, the developed acoustic detection system for early detecting HABs has demonstrated that it could be a significant system to monitor the occurrence of HABs in coastal regions.

• ALGAE
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• v.19 no.1
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• pp.7-14
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• 2004

Blue-Min was initially developed as an adsorbent for harmful gas removal and recently improved to apply to livestock, agriculture and aquaculture as an assistant feed. In the Blue-Min treatment, growth of harmful algae (Cochlodinium polykrikoides and the others causing the red-tide in the ocean) were inhibited below 10% in comparison with control and coagulation removal of harmful alge with Blue-Min treatment was more efficient than that of yellow loess treatment. It would be expected that the Ble-Min can be useful for the extirpator against the red-tide organisms and restrain the toxic algal growth around the fish aquaculture using the assistant feed. Recently, its utility has become to be diverse as it was revealed that aquaculture productivity increase by its application and, in addition, that it improve the water quality or sediment conditions in the aquaculture of Chinese White Shrimp. When Blue-Min was treated with the proper dose, the growth inhibition of Microcystis aeruginosa and lsochrysis galbana, which are typical red-tide organisms in freshwaters and food organisms in aquaculture, respectively, were less than that of marine red-tide organisms, while their growth slightly increased with low concentration treatiment. In addition, polyunsaturated fatty acids (PUFA) content of I. galbana slightly increase with the Blue-Min treatment. Through our research, the Blue-Min has diverse and comples function against various biological organisms and is proved as a biological activator or depressor.

• The Journal of the Korea institute of electronic communication sciences
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• v.16 no.6
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• pp.1363-1372
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• 2021

Red tide, defined as the major reproduction of harmful birds, has the characteristics of being generated and diffused in a wide area. This has limitations in detection only with the existing investigation method. Therefore, in this study, red tide was detected using a remote sensing technique. In addition, it was intended to increase the accuracy of detection by using optical characteristics, not just the concentration of chlorophyll. Red tide mainly occurs on the southern coast where sea signals are complex, and the main red tide control species on the southern coast is Cochlodinium polykirkoides. Therefore, it was intended to secure objectivity by reflecting features that could not be found depending on the researcher's observation and experience, not limited to visual judgment using machine learning techniques. In this study, support background machines and random forest were used among machine learning models, and as a result of calculating accuracy as performance evaluation indicators of the two models, the accuracy was 85.7% and 80.2%, respectively.

• Journal of Life Science
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• v.15 no.4 s.71
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• pp.578-583
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• 2005

Cell-free culture broth of marine halophilic bacterium, Kordia algicida was shown to possess specific algicidal ability against red tide organism, Cochlodinium polykrikides. Physiochemical characteristics of algicidal material originated in the bacterial culture broth were analyzed that its molecular weight was estimated to a 3,000 dalton and it was stable in heat and pH treatment. The algicidal fraction against C. polykrikoides obtained from gel permeable chromatography contained high concentration of ammonium ion as analyzed by ICP/Mass spectrum. C. polykrikoides by the fraction was quickly lysed within 1 min. It was shown that the effective concentration for algicide against C. polykrikoides was over 1mM of ammonium chloride. On the other hand, other metal ions presented in the algicidal fraction showed no algicidal effect against C. polykrikoides. In additon, ammonium ion exhibited species-specific killing spectrum for two species of red tide organisms, C. polykrikoides and Gymnodinium sanguieum. Therefore, further researches on the killing mechanism against C. polykrikoides exerted by ammonium ion, and subsequent development of replaceable algicidal materials will perform to provide useful tools for the control of red tide.

• ALGAE
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• v.32 no.4
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• pp.285-308
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• 2017

Cochlodinium polykrikoides red tides have caused great economic losses in the aquaculture industry in many countries. To investigate the roles of metazooplankton in red tide dynamics of C. polykrikoides in the South Sea of Korea, the abundance of metazooplankton was measured at 60 stations over 1- or 2-week intervals from May to November 2014. In addition, the grazing impacts of dominant metazooplankton on red tide species and their potential heterotrophic protistan grazers were estimated by combining field data on the abundance of red tide species, heterotrophic protist grazers, and dominant metazooplankton with data obtained from the literature concerning ingestion rates of the grazers on red tide species and heterotrophic protists. The mean abundance of total metazooplankton at each sampling time during the study was 297-1,119 individuals $m^{-3}$. The abundance of total metazooplankton was significantly positively correlated with that of phototrophic dinoflagellates (p < 0.01), but it was not significantly correlated with water temperature, salinity, and the abundance of diatoms, euglenophytes, cryptophytes, heterotrophic dinoflagellates, tintinnid ciliates, and naked ciliates (p > 0.1). Thus, dinoflagellate red tides may support high abundance of total metazooplankton. Copepods dominated metazooplankton assemblages at all sampling times except from Jul 11 to Aug 6 when cladocerans and hydrozoans dominated. The calculated maximum grazing coefficients attributable to calanoid copepods on C. polykrikoides and Prorocentrum spp. were 0.018 and $0.029d^{-1}$, respectively. Therefore, calanoid copepods may not control populations of C. polykrikoides or Prorocentrum spp. Furthermore, the maximum grazing coefficients attributable to calanoid copepods on the heterotrophic dinoflagellates Polykrikos spp. and Gyrodinium spp., which were grazers on C. polykrikoides and Prorocentrum spp., respectively, were 0.008 and $0.047d^{-1}$, respectively. Therefore, calanoid copepods may not reduce grazing impact by these heterotrophic dinoflagellate grazers on populations of the red tide dinoflagellates.

• Journal of Marine Life Science
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• v.7 no.2
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• pp.171-179
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• 2022

The present study investigated the survival rate, respiration rate, plasma stress index, and histological changes according to exposure time of cultured red seabream (Pagrus major) and olive flounder (Paralichthys olivaceus) exposed to Cochlodinium polykrikoides red tide. Fish cultured in natural seawater were used as the control group. Cochlodinium polykrikoides density was set to 5,500±200 cells·ml^-1 in the experimental groups. All red seabreams died within 1 hour of exposure to red tide, whereas all olive flounders died within 5 hours of exposure. Analysis of physiological response revealed that in red seabream, plasma glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), catalase (CAT), and glutathione peroxidase (GPx) concentrations were increased; plasma glucose and superoxide dismutase (SOD) concentration were decreased. Meanwhile, in olive flounders, plasma cortisol, GOT, and GPT concentrations were increased; plasma glucose concentrations were increased during the first hour of exposure, followed by decrease after 5 hours; and plasma SOD, CAT, and GPx concentrations decreased during the first hour of exposure. Histological analysis revealed structural damage to the gills of both red seabream and olive flounder. In conclusion, the exposure of red seabream and olive flounder to Cochlodinium polykrikoides red tide at the density of 5,500 cells·ml^-1 induces oxidative stress, which activates antioxidant defense mechanisms, ultimately leading to liver and gill damage.