• 대한본초학회지
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• 제20권4호
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• pp.1-10
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• 2005

Objectives : We investigated the anti-aging effect on skin with the extract of the needles of red pine, Pinus densiflora. Methods : We measured various effects related to skin such as the anti-oxidant effect, the protection against ultraviolet (UV) irradiation, the inhibition of reactive oxygen species (ROS) generation, the induction of heat shock protein 70 (HSP70), the reduction of matrix metalloproteinase-2 (MMP-2) synthesis and senescent cell. Results : The results were as follows : The extract of the needles of red pine (RP) had the potent anti-oxidant effect and the ROS scavenging effect. Also RP preserved the systemic anti-oxidant enzyme system (superoxide dismutase and catalase) from UVB irradiation. RP protected the cell membrane from the damages induced by UVB irradiation. RP induced HSP70, a mediator of resistance to UVB irradiation. RP reduced the synthesis of MMP-2 induced by UVB irradiation. And RP inhibited the amount of senescent-associated (SA) ${\beta}-galactosidase$ staining, as a marker of replicative senescence. Conclusions : The results of our study indicate that the extract of the needles of red pine, Pinus densiflora, has anti-aging effects on skin.

• Journal of the Korean Wood Science and Technology
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• 제24권2호
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• pp.81-93
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• 1996

To elucidate the structure of procyanidin in Korean red pine (Pinus densiflora S. et Z.), bark, the extractives were extracted with acetone-water mixture(7:3, v/v) from inner bark of Korean red pine. The extracts separated three fractions which were extracted by liquid-liquid extraction. The extracting solvents were chloroform and ethyl acetate and water. The part of ethylacetate soluble was chromatographed by liquid chromatography. The ethylacetate soluble portion yielded four natural procyanidin dimers, two known epicatechin-($4{\beta}{\rightarrow}6$)-catechin, catechin-($4{\alpha}{\rightarrow}8$)-catechin and two unknown catechin-($4{\beta}{\rightarrow}6$)-catechin and conformational isomer of epicatechin-($4{\alpha}{\rightarrow}6$)-catechin. The additional catechins was also isolated. The structures of these procyanidins were elucidated by their $^{13}C$-NMR spectra.

• Journal of the Korean Wood Science and Technology
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• 제21권1호
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• pp.39-50
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• 1993

The purpose of this study was to describe the micromorphological changes in Korean red pine (Pinus densiflora S. et Z.) wood decayed by a major brown-rot fungus, Lentinus lepideus, using scanning electron microscope and transmission electron microscope. At the end of the 12-week exposure to the fungus in soil block procedure(ASTM 1971), test blocks sustained 5.02% weight loss. The formation of bore hole by hyphae and penetration of hyphae through bordered pit were not observed. Instead, fungal hyphae appeared to penetrate axially tracheid luminar from the the ray cells via cross field pits. Hyphae were mainly found in lignin rich cell corner regions of tracheids, and also extensive degradation of tracheid wall occurred in this region. Extensive degradation of $S_2$ layer occurred without noticeable alteration of the $S_3$ layer, but warty layer and compound middle lamella remained relatively intact. Localized erosion, the characteristic of white rot, was observed in some cell wall and wall components including lignin were found to be decomposed.

• Journal of the Korean Wood Science and Technology
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• 제47권4호
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• pp.498-508
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• 2019

This study was carried out to investigate the potential of Korean red pine (Pinus densiflora) inner bark extracts as an antidiabetic agent. The ethyl acetate soluble fraction of the bark extracts was chromatographed on a Sephadex LH-20 column to yield five compounds, which structures were elucidated by NMR spectroscopy. The isolated compounds were (+)-catehin, (-)-epicatechin, taxifolin, taxifolin-3'-O-${\beta}$-D-(+)-glucose and $\tilde{n}$-courmaric acid. The antidiabetic activity of the different fractions, including the crude extracts and isolated compounds, was evaluated by ${\beta}$-cells insulin secretion and glucose uptake in skeletal muscle cells. The insulin secretion was 128% for taxifolin at $25{\mu}g/mL$. However, the other samples had no effect on this test. For the glucose uptake activity assay, $1{\mu}M$ insulin and 2 mM metformin were used as controls. Both the crude extract and taxifolin showed relatively low activity values, but the other samples yielded glucose uptake values over 260%. ${\rho}$-courmaric acid showed the highest uptake (270%). The results confirmed that Korean red pine extracts may be used as a hypoglycemic agent.

• 한국환경생태학회지
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• 제21권3호
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• pp.257-277
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• 2007

Z-M방법으로 한국 전역의 소나무림에 대한 식물사회학적 연구를 수행하였다. 소나무림은 크게 1군집, 3군락, 7아군락으로 구분되었다. 한국 소나무림의 분류체계는 다음과 같다. Rhododendro-Quercetalia mongolicae Kim 1990 I : Lindero-Quercion mongolicae Kim 1990 em. Kim 1992 A : Quercus mongolica-Pinus densiflora community A-1 : Typical subcommunity A-2 : Vaccinium koreanum subcommunity A-3 : Rhododendron micranthum subcommunity B : Quercus serrate-Pinus densiflora community B-1 : Typical subcommunity B-2 : Juniperus rigida subcommunity B-3 : Styrax japonica subcommunity B-4 : Eurya japonica subcommunity C: Saso-Pinetum densiflorae Yim et al. 1990 Camellietalia japonicae Oda et Sumata 1966 II : Ardisio-Castanopsion Miyawaki et al. 1971 D : Castanopsis cuspidata var. sieboldii-Pinus densiflora community 소나무-신갈나무군락은 한반도 중북부지역의 산지에 주로 분포하였으며, 소나무-졸참나무군락은 한반도 중부와 남부의 저산지와 구릉지에 넓게 나타났다. 소나무-제주조릿대군집은 제주도의 소나무림에서 조사되었다. 그리고 소나무-구실잣밤나무군락은 남서해안과 도서를 포함하는 난 온대역에 분포하였다.

• Journal of the Korean Wood Science and Technology
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• 제33권6호통권134호
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• pp.25-30
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• 2005

두께 50 mm 국산 소나무(Pinus densiflora Sieb. et Zucc.) 판재의 건조스케줄 개량을 위해 일반 열기 건조스케줄, 시간 기준 건조스케줄, 연속 온도상승 건조스케줄, 그리고 습구온도 조절 건조스케줄을 적용하여 최종함수율 15% 정도까지 건조한 후 각각의 건조스케줄 적용에 의한 건조특성을 비교분석하였다. 두께 50 mm 국산 소나무의 일반 열기 건조스케줄 적용실험 결과 건조속도는 0.53%/hr, 시간 기준 건조스케줄 적용에 의한 건조속도는 0.9%/hr, 그리고 연속 온도상승 건조스케줄 적용에 의한 건조속도는 2.29%/hr였으며, 습구온도 조절 건조스케줄 적용에 의한 건조속도는 1.52%/hr였다.

• Molecules and Cells
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• 제20권2호
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• pp.201-209
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• 2005

We have constructed an AFLP-based linkage map of Japanese red pine (Pinus densiflora Siebold et Zucc.) using haploid DNA samples of 96 megagametophytes from a single maternal tree, selection clone Kyungbuk 4. Twenty-eight primer pairs generated a total of 5,780 AFLP fragments. Five hundreds and thirteen fragments were verified as genetic markers with two alleles by their Mendelian segregation. At the linkage criteria LOD 4.0 and maximum recombination fraction 0.25(${\theta}$), a total of 152 markers constituted 25 framework maps for 19 major linkage groups. The maps spanned a total length of 2,341 cM with an average framework marker spacing of 18.4 cM. The estimated genome size was 2,662 cM. With an assumption of equal marker density, 82.2% of the estimated genome would be within 10 cM of one of the 230 linked markers, and 68.1% would be within 10 cM of one of the 152 framework markers. We evaluated map completeness in terms of LOD value, marker density, genome length, and map coverage. The resulting map will provide crucial information for future genomic studies of the Japanese red pine, in particular for QTL mapping of economically important breeding target traits.

• 한국환경생태학회지
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• 제23권1호
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• pp.56-65
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• 2009

한국에서 소나무는 문화적, 역사적, 정서적으로 중요한 위치에 있으며, 목재자원 생산이 가능한 수종이다. 소나무의 점유면적은 점차 줄어드는 반면, 참나무류를 비롯한 활엽수림이 증가하는 추세이므로 소나무림 유지를 위한 갱신과 시업법에 관한 연구가 요구된다. 본 연구는 중부지역을 대상으로 소나무에 대한 임분유형 및 구조분석을 통하여 생육환경과 천이과정을 파악함으로서, 안정적이며 지속적인 경영림으로서의 발달과 천연갱신 유도에 그 목적이 있다. 중부지역 소나무림은 당단풍나무군락, 상수리나무군락, 비목군락, 전형군락으로 분류되었으며, 지역에 따라 유형별 다른 특성을 보이고 있었다. 상층에는 소나무가 높게 나타나고 있으나, 중층에서는 참나무류 등 활엽 교목성 수종의 중요치가 높았으며, 하층에서 소나무 출현은 빈약하였다. 따라서 상관적으로 소나무가 생태적 영향력이 높은 것처럼 나타나고 있지만, 차후 자연 상태에서 교목층의 소나무가 고사하면 아교목층 이하 신갈나무, 굴참나무, 졸참나무 등 참나무류가 우점할 것으로 사료되었다. 소나무림의 형질과 생장패턴은 임분유형에 따라 지역별 차이를 보이고 있어 소나무림에 대한 시업도 차별 적용되어야 할 것으로 사료되었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.119-119
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• 2019

Pinus densiflora, the Korean Red Pine, is the predominant tree species of the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. Pinus densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones contain many bioactive phytochemicals, but they are rarely used as natural raw materials. This study was conducted to evaluated the anti-inflammatory effect of pine cone extracts and its possbility of natural sources were evaluated. Pine cones were extracted with 80% methanol, concentrated and then partitioned with ethyl acetate, and the organic layer was used as a sample. The Pine cone Ethyl acetate Fraction (PEF) showed no toxicity to RAW 264.7 cells at a concentration of less than $50{\mu}g/ml$. PEF inhibited the production of nitric oxide (NO) in RAW 264.7 cells treated with lipopolysaccharide (LPS). Also, It suppressed the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and transcription of nuclear factor-kappa B (NF-${\kappa}B$). These results suggest that pine cones can be used as an effective natural material for anti-inflammatory agent.

• 한국가구학회지
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• 제14권1호
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• pp.11-20
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• 2003

Wood cell-wall modification with acetic anhydride, lumen filled with styren monomer and methyl methacrylate, and a combination of these two treatments were studied for their effectiveness for dimensional stability. Compared to those of untreated Pinus densiflora Sieb. et Zucc and sole acetylated red pine, The combination of acetylation and impregnation with methy methacrylate greatly reduced water absorption, increased ASE to the best and gave better bending strength and compression strength.