• Journal of Microbiology and Biotechnology
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• v.2 no.2
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• pp.135-140
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• 1992

The partitioning of recombinant human interleukin-2(rhII-2) in PEG 8000-dextran 38800 aqueous two-phase system has been investigated using three different sources of rhIL-2. In the case of pure rhIL-2, the solubility in a PEG-dextran two-phase system was low and most of rhIL-2 was partitioned into the bottom phase. For the recovery of rhIL-2 from insoluble protein aggregates, the inclusion bodies of recombinant E. coli were solubilized by the treatment with sodium dodecyl sulfate (SDS). The addition of SDS significantly enhanced not only the solubility of rhIL-2 but also the partitioning of rhIL-2 to the top phase. When the ratio of SDS to rhIL-2 was 2.0, the partition coefficient(K) and the recovery yield(Y) at the top phase were 4.5 and 88%, respectively, at pH 6.8. In order to reduce the recovery steps further, SDS was directly added to the intact recombinant E. coli cells and then partitioned into the PEG/dextran aqueous two-phase system. The observed partition coefficient ($K{\cong{3.0$) and recovery yield ($Y{\geq}80%$ )of this method were comparable to the rhIL-2 recovery from insoluble protein aggregates. The results obtained in this work indicate that PEG-dextran two-phase partitioning might provide a simple way for the recovery and partial purification of recombinant proteins which are produced as inclusion bodies.

• Journal of applied mathematics & informatics
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• v.8 no.2
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• pp.327-343
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• 2001

This is a survey article on finite element a posteriori error estimates with an emphasize on gradient recovery type error estimators. As an example, the error estimator based on the ZZ patch recovery technique will be discussed in some detail.

• Journal of the Korea Institute of Military Science and Technology
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• v.24 no.6
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• pp.619-628
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• 2021

In order to compare numerical analyses made by Song and Kim needed for predicting gas and water filling with experimental results we conducted an experiment to recover a test projectile (43.7 kg with a 155 mm diameter) at a velocity of 775 m/s in a soft recovery system with a length of 179 m using pressurized gas and filled water. The soft recovery system consisting of a series of pressure tubes had a diaphragm, piston, and water plug for filling the pressurized gas and water. We installed a continuous wave Doppler radar system for velocity measurements of the test projectile travelling in the pressure tubes and pressure transducers for measuring the pressure in the soft recovery system. Continuous wave Doppler radar has the advantage of achieving real-time measurements of the velocity of a test projectile. The velocity-time curve of the test projectile, measured using the continuous wave Doppler radar, and the pressure profile were compared with the numerical analysis results. The experiment results show good agreement with the numerical analysis results based on the one-dimensional Euler equation with an HLL Riemann solver.

• Transactions of the Korean Society of Automotive Engineers
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• v.20 no.2
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• pp.21-29
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• 2012

A dual loop waste heat recovery system with Rankine steam cycles for the improvement of fuel efficiency of gasoline vehicles has been investigated. A high temperature loop (HT loop) only recovers the heat of the exhaust gas. A low temperature loop (LT loop) recovers the residual heat from the HT loop, the coolant heat and the remaining exhaust gas heat. The two separate loops are coupled with a heat exchanger. This paper has dealt with a layout of the dual loop system, the review of the working fluids, and the design of the cycle. The design point and the target heat recovery of the HT boiler, a core part of a HT loop, have been presented. The prototype of the HT boiler was evaluated by experiment. For the performance evaluation of the HT boiler, inlet temperature of the HT boiler working fluid was set equal to the temperature degree of sub-cool of $5^{\circ}C$ at the condensing pressure. The exit condition was the degree of super-heat set at $5^{\circ}C$. The characteristics of the HT boiler such as heat recovery and pressure drops of fluids were evaluated with varying flow rates and inlet temperatures of exhaust gas under various evaporating pressure conditions.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.30B no.12
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• pp.31-39
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• 1993

In the playback signal processing of a digital magnetic recording system, the major signal processing processes consist of pulse equalization. pulse detection, clock recovery, and data recovery. Equalizer which compensates interference occurrde between pulses recorded in high density on a magnetic media is realized by pulse slimming method, and pulse detection by a integrating detector. Clock recovery from the detector output was accomplished by using PLL. and data recovery to reduce noise effects was carried out by utilizing the three sampling clocks recovered in clock recovery process. In this paper these processes are implemented in hardware and its performance is evaluated by experimenting with a commercial DAT. It was found that the playback signal processor proposed is suitable to the practical high density magnetic recording system.

• The Transactions of the Korea Information Processing Society
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• v.7 no.6
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• pp.1874-1885
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• 2000

When a receiver of ciphertext message can not decrypt a message because he has lost his private-key, the private-key of receiver and session-key of the message should be recovered. In this paper, we developed an Encapsulation based Key Recovery System (EKRS). EKRS is a new key encapsulation based key recovery system which is characterized by secretly choice of KRA, randomized target keys. n-way recovery type, and useful for commercial key recovery. EKRS is formally specified by a pictorial model, an Extended Cryptographic Timed Petri Net (ECTPN). Secure information flow and reachability of a session-key are verified by using reachability graph of ECTPN. EKRS, executing over a Public Key Infrastructure, can be used as a security solution in Web based applications such as CALS, EC an EDI.

• Journal of Energy Engineering
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• v.18 no.1
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• pp.17-21
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• 2009

By using the heat recovery of water-cooled chillers, it is possible to reduce the energy operating costs positively and at the same time it could fulfill the heating re-heat air conditioning system as well as the hot water requirements. Basically templifiers are designed to economically to turn the waste heat into useful heat. Waste heat is extracted from a fluid stream by cooling it in the evaporator, the compressor amplifies the temperature of the heat and the condenser delivers the heat to heating loads such as space heating, kitchens and domestic hot water. Design of higher water temperature requirements and split condenser heat recovery chiller system (using of templifiers) produced hotter condenser water approximately up to $60^{\circ}C$ and control the entire heat recovery system.

• Transactions of the Korean Society of Automotive Engineers
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• v.19 no.5
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• pp.58-66
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• 2011

A 2-loop waste heat recovery system with Rankine steam cycles for the improvement of fuel efficiency of gasoline vehicles has been investigated. A high temperature loop(HT loop) is a system to recover the waste heat from the exhaust gas, a low temperature loop(LT loop) is for heat recovery from the engine coolant cold relatively. This paper has dealt with a layout of a LT loop system, the review of the working fluids, and the design of the cycle. The design point and the target heat recovery of the LT boiler, a core part of a LT loop, has been presented and analytically investigated. Considering the characteristics of the cycle, the basic concept of the LT boiler has been determined as a shell-and tube type counterflow heat exchanger, the performance characteristics for various design parameters were investigated.

• Journal of the Korea Institute of Military Science and Technology
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• v.23 no.5
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• pp.485-493
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• 2020

Numerical analyses were performed using a one-dimensional Euler equation and Godunov Harten-Lax-Van Leer(HLL) Riemann solver in order to study the deceleration characteristics of a 155 mm projectile in a soft recovery system. The soft recovery system consisting of a series of pressure tubes is a system that decelerates the test projectile fired at supersonic speed using a high-pressure gas and filled water inside. Therefore, depending on the gas pressure and the amount of water filling, the deceleration and the exit velocity of the test projectile inside the pressure tube are determined. In this paper, the deceleration characteristics of the test projectile were analyzed according to the gas pressure and water mass filled.

• Journal of Information Technology Services
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• v.11 no.3
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• pp.1-16
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• 2012

This paper is aimed to present a fuzzy decision-making approach to deal with disaster recovery priority decision problem in information system. We derive an evaluation approach based on TOPSIS(Technique for Order Performance by Similarity to Ideal Solution), to help disaster recovery priority decision of total information system for port logistics in a fuzzy environment where the vagueness and subjectivity are handled with linguistic terms parameterized by trapezoidal fuzzy numbers. This study applies the fuzzy multi-criteria decision-making method to determine the importance weight of evaluation criteria and to synthesize the ratings of candidate disaster recovery system. Aggregated the evaluators' attitude toward preference, then TOPSIS is employed to obtain a crisp overall performance value for each alternative to make a final decision. This approach is demonstrated with a real case study involving 4 evaluation criteria(system dependence, RTO, loss, alternative business support), 7 information systems for port logistics assessed by 5 evaluators from Maritime Affairs and Port Office.