• 한국콘텐츠학회논문지
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• 제18권4호
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• pp.254-267
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• 2018

본 연구는 부모 이혼 후 재혼(동거)가정 자녀의 계부모에 대한 호칭을 둘러싼 갈등을 자녀의 시각에서 탐색하고자 하였다. 이를 위해 심층면접을 활용한 질적 연구방법을 사용하였다. 연구참여자는 부모 이혼 후 재혼(동거)가정의 20대 자녀 4명이다. 자료 분석 결과, 자녀들이 계부모에게 아버지/어머니라 부르기 주저하는 이유는 '친부모가 있는데 왜 그분을 아버지/어머니라 불러야 하나요?', '아버지/어머니 없이 살아왔는데, 이제 와서 왜 필요하나요?', '그 분이 편하지 않아서 아버지/어머니라 부르지 않아요!'로 나타났다. 이러한 연구결과를 바탕으로 이혼 후 재혼(동거)가정 자녀의 계부모에 대한 호칭을 둘러싼 갈등 중재를 위한 상담의 지침을 제공하였다.

• 대한안전경영과학회지
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• 제16권2호
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• pp.101-109
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• 2014

This study is about safety education experience and practice of parents who are raising preschool children and the study results are as the following. For safety education experience state, parents had experience of safety education and mostly received it through communities, TV, Internet, etc. The most common educational content was the traffic safety education. For safety education practice degree according to number of children, safety education practice was mostly done well with 1~2 children. For safety education practice degree according to family type, safety education practice degree within the family was done most in nuclear families while healthy life habit safety education practice degree was done most in nuclear families and reconstituted families. Also, for traffic safety education practice degree, it was done well in nuclear families. For respiratory disease safety education practice degree, nuclear families and reconstituted families were the highest and for first aid safety education practice degree, single-parent families were the highest. For safety knowledge level of parents, safety knowledge of family was the highest, followed by safety knowledge of respiratory disease, and traffic. For safety education practice degree and safety knowledge according to safety education experience of parents, there was a significant difference in practice degree according to safety education experience of parents and there was also difference in practice knowledge according to experience. There was also education effect in healthy life habit safety knowledge and traffic safety knowledge.

• 가정과삶의질연구
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• 제21권1호
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• pp.25-36
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• 2003

This study was conducted to reconstitute a family life education program for caregivers and to analyze the system of implementation. Based largely on a pre-existing program for caregiving families, I developed a new educational program. Personal time management and personal needs management were added in the content of the program order to help enhance self-esteem and internal control. The reconstituted educational program, which consists of 6 sessions, was implemented on a small group of caregivers in Jeollabukdo Province who support their elderly parents. The effectiveness of the program was evaluated by a pre-test, a post-test, a follow-up test and open-questionnaire reports. The results from the post-test suggest that the program has been effective in improving the knowledge on aging process, internal control and self-esteem. But the follow-up test results show the program has been effective only in improving the knowledge on aging process. According to the open-questionnaire reports, the program seems to have been effective in improving interpersonal relationships and changing attitudes toward their elderly parents.

• 한국발생생물학회지:발생과생식
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• 제17권1호
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• pp.9-16
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• 2013

Coactivator-associated arginine methyltransferase 1 (CARM1) is included in the protein arginine methyltransferase (PRMT) family, which methylates histone arginine residues through posttranslational modification. It has been proposed that CARM1 may up-regulate the expression of pluripotency-related genes through the alteration of the chromatin structure. Mouse embryonic stem cells (mESCs) are pluripotent and have the ability to self-renew. The cells are mainly used to study the genetic function of novel genes, because the cells facilitate the transmission of the manipulated genes into target mice. Since the up-regulated methylation levels of histone arginine residue lead to the maintenance of pluripotency in embryos and stem cells, it may be suggested that CARM1 overexpressing mESCs elevate the expression of pluripotency-related genes in reconstituted embryos for transgenic mice and may resist the differentiation into trophectoderm (TE). We constructed a fusion protein by connecting CARM1 and 7X-arginine (R7). As a cell-penetrating peptide (CPP), can translocate CARM1 protein into mESCs. CPP-CARM1 protein was detected in the nuclei of the mESCs after a treatment of 24 hours. Accordingly, the expression of pluripotency-related genes was up-regulated in CPP-CARM1-treated mESCs. In addition, CPP-CARM1-treated mESC-derived embryoid bodies (EBs) showed an elevated expression of pluripotency-related genes and delayed spontaneous differentiation. This result suggests that the treatment of recombinant CPP-CARM1 protein elevates the expression of pluripotency-related genes of mESCs by epigenetic modification, and this protein-delivery system could be used to modify embryonic fate in reconstituted embryos with mESCs.

• 생명과학회지
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• 제22권11호
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• pp.1456-1462
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• 2012

소라페닙은 멀티카이네즈 억제제로서 신세포암, 전이성 간세포암 환자의 치료에 효과가 입증된 경구용 항암제이다. 이 연구의 목적은 고속액체크로마토그래피 텐덤질량분석기법(LC/MS/MS)을 이용하여 사람 혈장 내 소라페닙의 농도를 측정하는 효율적인 방법을 개발하고 한국식품의약품안전청(KFDA) 기준에 따라 분석법을 검정하는 것이다. 혈장시료($100{\mu}l$)에 내부표준물질인 chlorantraniliprole을 첨가한 후 이소프로필알콜과 에틸아세테이트로 구성(1:4, v/v)된 0.1% 포름산 함유 추출용액을 혼합하였다. 원심분리 후 상층액을 취하여 원심감압농축하였다. 잔사를 이동상에 재용해하고 Waters사의 역상 XTerra$^{TM}$ C18 칼럼(입자크기 $3.5{\mu}m$)을 장착한 고속액체크로마토그래피 장치에 주입하였다. 액체크로마토그래피는 0.1% 포름산과 10 mM 암모늄 포메이트를 함유한 버퍼용액과 메탄올, 아세토나이트릴을 각각 1:6:3으로 혼합한 용액을 이동상으로 사용하였으며 5분 내에 측정을 완료하였다. 분석대상 물질들은 텐덤질량분석기에서 electrospray 양이온 이온화($ES^+$) 검출방식으로 확인하였으며 소라페닙은 'm/z 465.2 ${\rightarrow}$ 252.5', chlorantraniliprole은 'm/z 484.4 ${\rightarrow}$ 286.2'으로 구성한 multiple reaction monitoring 방법을 사용하였다. 검정 결과, 2-5,000 ng/ml의 농도 구간에서 양호한 직선성($r^2$ > 0.99)과 정확도(90.7-103.9%), 정밀도(10% 이하)를 나타내었다. 새롭게 개발된 LC/MS/MS을 이용한 사람 혈장 내 소라페닙의 농도 측정법은 KFDA 기준을 만족하였으며, 기존의 방법에 비해 민감도가 높은 방법이었다.