• The Plant Pathology Journal
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• 제25권4호
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• pp.422-428
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• 2009

Studies were conducted to identify the sources of resistance in mungbean recombinant inbred lines (RILs) in Thailand against mungbean yellow mosaic disease (MYMD). 146 mungbean RILs in $F_8$ series were evaluated in a field including resistant parent NM-10-12-1 and susceptible parent KPS 2 during summer 2008 under high inoculum pressure. The RILs were subsequently scored for disease symptom severity ratings (DSSR) using a new scale. Observations regarding DSSR and % disease index (%DI) showed that the tested RILs responded differently to the disease. A large number of RILs (132) were found highly susceptible, 12 were susceptible, 3 were tolerant and one was resistant. Overall screening results showed that three RILs, viz. line no. 30, 100 and 101 had minimum DSSR and % disease index thus they are good source of resistance to MYMD in spite of high disease pressure and can therefore be used directly as varieties to manage the disease in Thailand.

• 한국작물학회지
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• 제44권1호
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• pp.86-94
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• 1999

A set of rice recombinant inbred lines was developed from a cross between a Tongil type variety, Nonganbyeo, and an indica variety, BG276, by the single seed descent method. The number of the lines in the population was 272. All the agronomic characters studied except ADV (alkali-digestion value) showed continuous variation among the RILs, implying that their inheritance mode should be quantitative. The patterns of the variation in the RILs were either normal or skewed distribution. ADVs of RILs were segregated into two groups with 1:1 ratio, indicating that ADVs in this KIL population might be controlled by one major gene. Transgressive variations were also observed in all characters. Heritability values of the characters varied from 0.488 in brown/rough rice ratio to 0.895 in alkali-digestion value. In the analysis of genotypic and phenotypic correlations, the character of yield was positively correlated with 8 different agronomic characters. The number of panicles per hill was negatively correlated with culm length, panicle length, and number of spikelets per panicle. Grain length was positively correlated with grain width, grain thickness, grain length/width ratio, white belly, ADV, and amylose. However, grain length/width ratio was negatively correlated with grain width. White core was also negatively correlated with white belly and ADV.

• 한국자원식물학회지
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• 제33권6호
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• pp.615-623
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• 2020

Brassica rapa is one of the most valuable vegetable crops worldwide. Cultivated varieties of B. rapa exhibit diverse developmental and morphological appearances, which includes important vegetables, oilseeds, and fodder crops. In this study, various phenotypes of recombinant inbred lines (RILs) of B. rapa were investigated, including their responses to five different pathogenic Botrytis cinerea isolates, responses to aphid and thrips during flowering stages, days to flowering, and plant heights. Responses of 113 RILs to five different B. cinerea isolates showed variations, suggesting that genetic factors controlling resistance or tolerance against each isolate were dependent on isolate/genotype pairs. Correlation analysis was performed to understand the nature of genetic factors and the relationship among these phenotypes. Although high levels of correlation were not detected between phenotypes assessed in this study, statistically significant correlation was detected for several combinations. Significant positive correlations were found for different B. cinerea isolates, supporting that certain levels of commonality could exist in genetic components controlling resistance against different B. cinerea isolates. Based on correlation analysis using numbers of insects counted on plants, it was speculated that genetic factors responsible for aphid tolerance or repellence might be also involved in the response against thrips. Relationship between vegetative growth and tolerance against B. cinereal or insects is rather more complicated. However, it was observed that shorter plants appeared to have a certain level of tolerance or repellence against both aphids and thrips. Data presented in this study could be used to assist further genetic studies and breeding efforts to obtain Botritis and insect resistance for B. rapa.

• Plant Breeding and Biotechnology
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• 제5권4호
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• pp.371-389
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• 2017

Supplying sufficient rice to growing populations is a global challenge. Hybrid indica rice varieties exploiting heterosis have increased yields, but inter-subspecific crosses between indica and japonica varieties are hampered by sterility. Examination and genetic understanding of yield heterosis in indica/japonica crosses addressing yield barriers are basic requirements. In this study, QTLs for heterosis of yield traits were identified in indica-japonica recombinant inbred lines (RILs) using a total of 178 RILs originating from Dasanbyeo (indica) ${\times}$ TR22183 (japonica) (DT-RILs) and their backcrossed populations. Nine of sixty-six major quantitative trait loci (QTLs) identified in DT-RILs exhibited heterosis. Heterosis QTLs clustered with other traits on chromosomes 1, 4, and 8, and clusters were conserved between different RILs. The clusters contained several known yield enhancement genes/QTLs. Specific heterotic allele combinations contributed to four major heterosis QTLs, particularly for panicle and spikelet number traits. Heterosis for yield and yield-related traits was explained by the harmonized effects of overdominance, dominance, and epistatic interactions in inter-subspecific breeding populations.

• 한국작물학회지
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• 제51권6호
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• pp.565-570
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• 2006

The viviparous germination (VG) with lodging caused the yield reduction and quality deterioration in rice. We carried out the evaluation of VG tolerance (on the 40th day after heading) and mapping QTLs associated with VG tolerance using the recombinant inbred lines (M/G RILs) from a cross between Milyang 23 (japonica/indica) and Gihobyeo (japonica). The VG rates of Milyang 23 and Gihobyeo were 0.0 and 7.0%, respectively. The averaged VG rate of 162 M/G RILs was 7.7%, and their range was from 0.0 to 50.9%. Of the 162 RILs, 144 lines were tolerant less than 10%, and 18 lines were susceptible more than 10%. Using the M/G RIL Map, three QTLs associated with the viviparous trait were detected on chromosome 2 (qVG 2-1 and qVG 2-2) and 8 (qVG 8). qVG 2-1 was linked to RM 32D and RZ 166, and had LOD score of 2.97. qVG 2-2 was tightly linked to E13M59.119-Pl and E13M59.M003-P2, and showed higher LOD score of 3.41. qVG 8 was linked to RM33 and TCT116, and had LOD score of 2.67. The total phenotypic variance explained by the three QTLs was about 24.4% of the total variance in the population. The detection of new QTLs associated with VG tolerance will provide important informations for the seed dormancy, low temperature germination, or comparative genetics.

• Molecules and Cells
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• 제21권2호
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• pp.192-196
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• 2006

Salt tolerance was evaluated at the young seedling stage of rice (Oryza sativa L.) using recombinant inbred lines (MG RILs) from a cross between Milyang 23 (japonica/indica) and Gihobyeo (japonica). 22 of 164 MG RILs were classified as tolerant with visual scores of 3.5-5.0 in 0.7% NaCl. Interval mapping of QTLs related to salt tolerance was conducted on the basis of the visual scores at the young seedling stage. Two QTLs, qST1 and qST3, conferring salt tolerance, were detected on chromosome 1 and 3, respectively, and the total phenotypic variance explained by the two QTLs was 36.9% in the MG RIL population. qST1 was the major QTL explaining 27.8% of the total phenotypic variation. qST1 was flanked by Est12~RZ569A, and qST3 was flanked by RG179~RZ596. The detection of new QTLs associated with salt tolerance will provide important information for the functional analysis of rice salt tolerance.

• Journal of Plant Biotechnology
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• 제29권2호
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• pp.135-138
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• 2002

대두 85개 재조합순계주의 미숙배로부터 얻은 자엽절편을 MS salt, B5비타민, 40 mg$^{-1}$ 2,4-dichlorophenoxyacetic acid (2,4-D) 및 30 g$^{-1}$ sucrose로 조성된 체세포배유도배지 (SIM, somatic induce medium)에 치상하여 5주간 배양하였다. 10%이하의 체세포배형성빈도는 36계통에서, 11~49%의 형성빈도는 37계통에서, 50~80%의 형성빈도는 9계통에서 그리고 90% 이상의 형성빈도는 3계통에서 각각 관찰되었다. 90% 이상의 체세포배형성빈도와 자엽당 6.36개의 체세포배를 형성하는 계통으로는 KM1010, KM1032 및 KM1064이었으며, 3계통에서 얻은 일차배를 동일배지상에서 계대배양하면서 2차배 증식방법으로 1년 이상 증식 유지하였다. 체세포배를 성숙배지와 식물체전환배지 (Komatsuda 1992)에 옮겼을 때 약 25% 정도가 정상식물체로 생육하였다.

• 한국작물학회지
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• 제51권spc1호
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• pp.58-66
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• 2006

통일형인 밀양23호와 자포니카인 기호벼를 교잡한 재조합자식계통을 대상으로 품질관련 특성 변이를 살펴본 결과 공시계통들에 대한 품질 관련 형질들의 변이 분포는 매우 폭 넓고 다양했으며 대부분의 형질에서 연속적인 정규분포를 보였다. 조사된 형질간의 상관관계는 립의 두께에 대하여 립폭, 심백과 복백은 고도의 정의 상관관계를 나타냈으나 심백과 알카러 붕괴도는 고도의 부의 상관관계를 보였다. 단백질 함량과는 아밀로스, Mg/K 비율에서 고도의 부의 상관을 나타냈으며, K와 지방 함량과는 고도의 정의 상관을 보였다. 밥의 물리성에서는 딱딱한 정도를 나타내는 경도와 부착성, 탄력성, 검성, 저작성에서 고도의 정의 상관을 보였다.

• Plant Biotechnology Reports
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• 제5권2호
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• pp.135-146
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• 2011

The objective of the present research was to map QTLs associated with agronomic traits such as days from sowing to flowering, plant height, yield and leaf-related traits in a population of recombinant inbred lines (RILs) of sunflower (Helianthus annuus). Two field experiments were conducted with well-irrigated and partially irrigated conditions in randomized complete block design with three replications. A map with 304 AFLP and 191 SSR markers with a mean density of 1 marker per 3.7 cM was used to identify QTLs related to the studied traits. The difference among RILs was significant for all studied traits in both conditions. Three to seven QTLs were found for each studied trait in both conditions. The percentage of phenotypic variance ($R^2$) explained by QTLs ranged from 4 to 49%. Three to six QTLs were found for each yield-related trait in both conditions. The most important QTL for grain yield per plant on linkage group 13 (GYP-P-13-1) under partial-irrigated condition controls 49% of phenotypic variance ($R^2$). The most important QTL for 1,000-grain weight (TGW-P-11-1) was identified on linkage group 11. Favorable alleles for this QTL come from RHA266. The major QTL for days from sowing to flowering (DSF-P-14-1) were observed on linkage group 14 and explained 38% of the phenotypic variance. The positive alleles for this QTL come from RHA266. The major QTL for HD (HD-P-13-1) was also identified on linkage group 13 and explained 37% of the phenotypic variance. Both parents (PAC2 and RHA266) contributed to QTLs controlling leaf-related traits in both conditions. Common QTL for leaf area at flowering (LAF-P-12-1, LAF-W-12-1) was detected in linkage group 12. The results emphasise the importance of the role of linkage groups 2, 10 and 13 for studied traits. Genomic regions on the linkage groups 9 and 12 are specific for QTLs of leaf-related traits in sunflower.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.27-27
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• 2022

(-)-Epicatechin (EC), a primary form of flavan-3ol and a building block of proanthocyanidins, has health benefits as it is a potent antioxidant. So far, no quantitative trait loci (QTLs) associated with EC have yet been identified in soybean. In this study, QTLs for EC and hilum color were identified in recombinant inbred lines (RILs) derived from the varieties Jinpung and IT109098 using high-resolution single nucleotide polymorphism linkage mapping. This revealed two major QTLs for EC content, qEC06 and qEC08. qEC06 spanned the T Locus encoding flavonoid 3'-hydroxylase. qEC08, located near the I locus on Chr08, was also a major QTL for hilum color; however, allelic stacking of qEC08 and I revealed no relationship between I and EC content. RILs with IT 109098 alleles at both qEC06 and qEC08 had higher EC content than other lines. These results will enable the production of soybean varieties with high EC content via marker-assisted selection.