Browse > Article
http://dx.doi.org/10.7732/kjpr.2020.33.6.615

Investigation of Defense and Vegetative Growth Related Traits of Recombinant Inbred Lines of Brassica rapa  

Kwon, Soon-Tae (Department of Horticulture and Breeding, Andong National University)
Yeam, Inhwa (Department of Horticulture and Breeding, Andong National University)
Shin, Jong Hwa (Department of Horticulture and Breeding, Andong National University)
Publication Information
Korean Journal of Plant Resources / v.33, no.6, 2020 , pp. 615-623 More about this Journal
Abstract
Brassica rapa is one of the most valuable vegetable crops worldwide. Cultivated varieties of B. rapa exhibit diverse developmental and morphological appearances, which includes important vegetables, oilseeds, and fodder crops. In this study, various phenotypes of recombinant inbred lines (RILs) of B. rapa were investigated, including their responses to five different pathogenic Botrytis cinerea isolates, responses to aphid and thrips during flowering stages, days to flowering, and plant heights. Responses of 113 RILs to five different B. cinerea isolates showed variations, suggesting that genetic factors controlling resistance or tolerance against each isolate were dependent on isolate/genotype pairs. Correlation analysis was performed to understand the nature of genetic factors and the relationship among these phenotypes. Although high levels of correlation were not detected between phenotypes assessed in this study, statistically significant correlation was detected for several combinations. Significant positive correlations were found for different B. cinerea isolates, supporting that certain levels of commonality could exist in genetic components controlling resistance against different B. cinerea isolates. Based on correlation analysis using numbers of insects counted on plants, it was speculated that genetic factors responsible for aphid tolerance or repellence might be also involved in the response against thrips. Relationship between vegetative growth and tolerance against B. cinereal or insects is rather more complicated. However, it was observed that shorter plants appeared to have a certain level of tolerance or repellence against both aphids and thrips. Data presented in this study could be used to assist further genetic studies and breeding efforts to obtain Botritis and insect resistance for B. rapa.
Keywords
Brassica rapa; Botrytis cinerea; Flowering time; Insect resistance; Recombinant inbred lines (RILs); Resistance;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Jones, A.M.E., M. Bridges, A.M. Bones, R. Cole and J.T. Rossiter. 2001. Purification and characterisation of a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.). Insect Biochem. Mol. Biol. 31:1-5.   DOI
2 Karasov, T.L., E. Chae, J.J. Herman and J. Bergelson. 2017. Mechanisms to mitigate the trade-off between growth and defense. Plant Cell 29:666-680.   DOI
3 Kim, J., D.S. Kim, S. Park, H.E. Lee, Y.K. Ahn, J.H. Kim, H.B. Yang and B.C. Kang. 2016. Development of a high-throughput SNP marker set by transcriptome sequencing to accelerate genetic background selection in Brassica rapa. Hortic. Environ. Biotechnol. 57:280-290.   DOI
4 Kliebenstein, D.J., H.C. Rowe and K.J. Denby. 2005. Secondary metabolites influence Arabidopsis/Botrytis interactions: Variation in host production and pathogen sensitivity. Plant J. 44:25-36.   DOI
5 Koornneef, M., C. Alonso-Blanco and D. Vreugdenhil. 2004. Naturally occurring genetic variation in Arabidopsis thaliana Annu. Rev. Plant. Biol. 55:141-172.   DOI
6 Li, X., W. Wang, Z. Wang, K. Li, Y. Lim and Z. Piao. 2015. Construction of chromosome segment substitution lines enables QTL mapping for flowering and morphological traits in Brassica rapa. Front. Plant Sci. 6:432.   DOI
7 Powell, G., C.R. Tosh and J. Hardie. 2005. Host plant selection by aphids: Behavioral, evolutionary, and applied perspectives. Annu. Rev. Entomol. 51:309-330.   DOI
8 Lou, P., J. Zhao, J. Kim, S. Shen, C. Del, X. Song, M. Jin, D. Vreugdenhil, X. Wang, M. Koornneef and G. Bonnema. 2007. Quantitative trait loci for flowering time and morphological traits in multiple populations of Brassica rapa J. Exp. Bot. 58:4005-4016.   DOI
9 Mason, A.S. and R.J. Snowdon. 2016. Oilseed rape: Learning about ancient and recent polyploid evolution from a recent crop species. Plant Biol. 18:883-892.   DOI
10 Mitchell, C., R.M. Brennan, J. Graham and A.J. Karley. 2016. Plant defense against herbivorous pests: Exploiting resistance and tolerance traits for sustainable crop protection. Front. Plant Sci. 7:1132.
11 Roux, F., P. Touzet, J. Cuguen and C.V. Le. 2006. How to be early flowering: an evolutionary perspective. Trends Plant Sci. 11:375-381.   DOI
12 Vanous, A., C. Gardner, M. Blanco, A. Martin-Schwarze, A.E. Lipka, S. Flint-Garcia, M. Bohn, J. Edwards and T. Lubberstedt. 2018. Association mapping of flowering and height traits in germplasm enhancement of maize doubled haploid lines. Plant Genome 11(2):1-14.
13 Amasino, R.M. 1996. Control of flowering time in plants. Curr. Opin. Genet. Dev. 6:480-487.   DOI
14 Amselem, J., C.A. Cuomo, J.A.L. Van Kan, M. Viaud, E.P. Benito, A. Couloux and et al. 2011. Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea. PLoS Genet. 7:e1002230.   DOI
15 Rowe, H.C. and D.J. Kliebenstein. 2008. Complex genetics control natural variation in Arabidopsis thaliana resistance to Botrytis cinerea. Genetics 180:2237-2250.   DOI
16 Shea, D.J., E. Itabashi, S. Takada, E. Fukai, T. Kakizaki, R. Fujimoto and K. Okazaki. 2017. The role of FLOWERING LOCUS C in vernalization of Brassica: The importance of vernalization research in the face of climate change. Crop Pasture Sci. 69:30-39.
17 Song, Y.H., I. Lee, S.Y. Lee, T. Imaizumi and J.C. Hong. 2012. CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis. Plant J. 69:332-342.   DOI
18 Staal, J., M. Kaliff, E. Dewaele, M. Persson and C. Dixelius. 2008. RLM3, a TIR domain encoding gene involved in broad-range immunity of Arabidopsis to necrotrophic fungal pathogens. Plant J. 55:188-200.   DOI
19 Staats, M. and J.A.L. van Kan. 2012. Genome update of Botrytis cinerea strains B05.10 and T4. Eukaryot. Cell 11:1413-1414.   DOI
20 van Wees, S. 2008. Phenotypic analysis of Arabidopsis mutants: Trypan blue stain for fungi, oomycetes, and dead plant cells. CSH Protoc. 2008(8), doi: 10.1101/pdb.prot4982.   DOI
21 Williams, P.H. and C.B. Hill. 1986. Rapid-cycling populations of brassica. Science 232:1385-1389.   DOI
22 Williamson, B., B. Tudzynski, P. Tudzynski and J.A. Van Kan. 2007. Botrytis cinerea: the cause of grey mould disease. Mol. Plant Pathol. 8:561-580.   DOI
23 Zhang, W., S.T. Kwon, F. Chen and D.J. Kliebenstein. 2016. Isolate dependency of Brassica rapa resistance QTLs to Botrytis cinerea. Front. Plant Sci. 7:161.   DOI
24 Bragard, C., P. Caciagli, O. Lemaire, J.J. Lopez-Moya, S. MacFarlane, D. Peters, P. Susi and L. Torrance. 2013. Status and prospects of plant virus control through interference with vector transmission. Annu. Review Phytopathol. 51:177-201.   DOI
25 Bhatia, V., P.L. Uniyal and R. Bhattacharya. 2011. Aphid resistance in Brassica crops: Challenges, biotechnological progress and emerging possibilities. Biotechnol. Adv. 29:879-888.   DOI
26 Bloomer, R.H. and C. Dean 2017. Fine-tuning timing: Natural variation informs the mechanistic basis of the switch to flowering in Arabidopsis thaliana. J. Exp. Bot. 68:5439-5452.   DOI
27 Bolton, M.D., B.P. Thomma and B.D. Nelson. 2006. Sclerotinia sclerotiorum (Lib.) de bary: Biology and molecular traits of a cosmopolitan pathogen. Mol. Plant Pathol. 7:1-16.   DOI
28 Campos, M.L., Y. Yoshida, I.T. Major, D. de Oliveira Ferreira, S.M. Weraduwage, J.E. Froehlich, B.F. Johnson, D.M. Kramer, G. Jander, T.D. Sharkey and G.A. Howe. 2016. Rewiring of jasmonate and phytochrome B signalling uncouples plant growth-defense tradeoffs. Nat. Commun. 7:12570.   DOI
29 Chouard, P. 1960. Vernalization and its relations to dormancy. Annu. Rev. Plant Physiol. 11:191-238.   DOI
30 Denby, K.J., P. Kumar and D.J. Kliebenstein. 2004. Identification of Botrytis cinerea susceptibility loci in Arabidopsis thaliana. Plant J. 38:473-486.   DOI
31 De Vos, M., J.H. Kim and G. Jander. 2007. Biochemistry and molecular biology of Arabidopsis -aphid interactions. Bio Essays 29:871-883.
32 Diaz-Montano, J., M. Fuchs, B.A. Nault, J. Fail and A.M. Shelton. 2011. Onion thrips (Thysanoptera: Thripidae): a global pest of increasing concern in onion. J. Econ. Entomol. 104:1-13.   DOI
33 Howe, G.A. and G. Jander. 2008. Plant immunity to insect herbivores. Annu. Rev. Plant Biol. 59:41-66.   DOI
34 Zust, T. and A.A. Agrawal. 2017. Trade-offs between plant growth and defense against insect herbivory: An emerging mechanistic synthesis. Annu. Rev. Plant Biol. 68:513-534.   DOI
35 Zhao, J., X. Wang, B. Deng, P. Lou, J. Wu, R. Sun, Z. Xu, J. Vromans, M. Koornneef and G. Bonnema. 2005. Genetic relationships within Brassica rapa as inferred from AFLP fingerprints. Theor. Appl. Genet. 110:1301-1314.   DOI
36 FitzJohn, R.G., T.T. Armstrong, L.E. Newstrom-Lloyd, A.D. Wilton and M. Cochrane. 2007. Hybridisation within Brassica and allied genera: evaluation of potential for transgene escape. Euphytica 158:209-230.   DOI
37 Goggin, F.L. 2007. Plant-aphid interactions: molecular and ecological perspectives. Curr. Opin. Plant Biology 10:399-408.   DOI
38 Guo, Y., S. Chen, Z. Li and W.A. Cowling. 2014. Center of origin and centers of diversity in an ancient crop, Brassica rapa (turnip rape) J. Hered. 105:555-565.   DOI
39 Hogenhout, S.A., E.D. Ammar, A.E. Hitfield and M.G. Redinbaugh. 2008. Insect vector interactions with persistently transmitted viruses. Annu. Rev. Phytopathol. 46:327-359.   DOI
40 Hopkins, R.J., N.M. van Dam and J.J.A. van Loon. 2009. Role of glucosinolates in insect-plant relationships and multitrophic interactions. Annu. Rev. Entomol. 54:57-83.   DOI
41 Huot, B., J. Yao, B.L. Montgomery and S.Y. He. 2014. Growthdefense tradeoffs in plants: a balancing act to optimize fitness. Mol. Plant 7:1267-1287.   DOI
42 Iniguez-Luy, F.L., L. Lukens, M.W. Farnham, R.M. Amasino and T.C. Osborn. 2009. Development of public immortal mapping populations, molecular markers and linkage maps for rapid cycling Brassica rapa and B. oleracea. Theor. Appl. Genet.120:31-43.   DOI