• Journal of Pharmaceutical Investigation
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• v.31 no.3
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• pp.185-190
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• 2001

Human papillomavirus (HPV) infection is known to cause cervical cancers. Human papillomavirus-like particles (VLP) have been studied as preventive vaccines of cervical cancers. To develop VLP as a therapeutic gene carrier, we studied the method to encapsulate cytokine genes in virus-like particles. HPV type 16 capsid L1 genes were amplified by polymerase chain reaction and cloned into T vector. L1 gene was then inserted into baculovirus transfer vector. The clone of baculovirus encoding L1 gene was isolated and used to express L1 protein in Sf 21 insect cells. VLP were purified by CsCl density gradient and ultracentrifugation. VLP were disassembled to capsomer units by treatment of a reducing agent. Given that interleukin-2 (IL-2) genes have been used in anticancer gene therapy and as a molecular adjuvant, IL-2 cytokine plasmids were chosen as a model gene. IL-2 plasmids were incubated with the disassembled capsomer suspension. To reassemble the particles, the mixture of capsomers and cytokine plasmids was dialyzed. The disassembly and reassembly of VLP were confirmed by transmission electron microscopy. The entrapment of cytokine plasmids in reassembled VLP was tested by the stability of plasmids against DNase I. After treatment of reassembled virus-like particles with DNase I, discrete IL-2 DNA band was observed. Our results indicate that IL-2 cytokine plasmid (3.5 kb size) can be encapsulated in the virus-like particles, suggesting the potential of VLP as a gene delivery system. Moreover, VLP containing the adjuvant cytokine plasmids might function as more effective subunit vaccines.

• Conservation Science in Museum
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• v.20
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• pp.1-12
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• 2018

In February 1986, a high-school student reported the discovery of fragments of gilt-bronze burial goods on a mountainside behind a village in Ipjeom-ri, Ungpo-myeon in Iksan. The National Research Institute of Cultural Heritage immediately undertook an excavation. Unfortunately, gilt-bronze relics including a band and vertical decorations for a crown, a hat, and shoes had already been collected from Ipjeom-ri Tomb 1 by the student who found them before they could be properly excavated. Also, the interior of the stone chamber of the tomb had been severely disturbed by the time of excavation, making it difficult to identify the precise original locations of the relics within the tomb and hindering the reassembly of the fragments of the gilt-bronze crown. After conservation treatment, the gilt-bronze hat, shoes, and other relics were restored to their original forms and researchers were able to identify incised designs of a three-legged bonghwang(鳳凰), fish scales, lotus flowers, and other motifs. This study presents the major features of the fragments of gilt-bronze relics from Ipjeom-ri Tomb 1 and the methods of their production in order to provide basic material for their restoration and allow the eventual restoration of the giltbronze crown to its original condition.

• Journal of KIISE:Computing Practices and Letters
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• v.14 no.4
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• pp.389-400
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• 2008

As the wireless network technologies and the capability of the mobile terminals are evolving, advanced peer to peer applications for mobile users are attracting interests. In this paper, we propose the mobile P2P communication platform(MPCP) which provides transparency to the wireless network technologies and solutions to the limited resources of the mobile terminals. MPCP classifies the connection into two levels: a virtual channel and a session. A virtual channel is the network layer connection between the terminals whereas a session is the application layer connection. MPCP classifies the sessions into four types and applies different scheduling priority and data processing policies such as segmentation and reassembly. It selects proper wireless network technologies depending on the distance between the communication endpoints. To acquire dynamically changed access address, we harness the Session Initiation Protocol. We implemented MPCP on embedded Linux simulator and utilized the implementation in mobile P2P service development. For the quantitative analysis, we compared the performance of MPCP with that of ftp. Regardless of the number of simultaneous sessions, MPCP maintains the relative performance.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.46 no.5
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• pp.37-43
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• 2009

Streaming video service over wireless and mobile communication networks has received significant interests from both academia and industry recently. Specifically, mobile WiMAX (IEEE 802.16e) is capable of providing high data rate and flexible Quality of Service (QoS) mechanisms, supporting mobile streaming very attractive. However, we need to note that streaming videos can be partially deteriorated in their macroblocks and/or slices owing to errors on OFDMA subcarriers, as we consider that compressed video sequence is generally sensitive to the error-prone channel status of the wireless and mobile network. In this paper, we introduce an OFDMA subcarrier-adaptive mobile streaming server based on cross-layer design. This streaming server system is substantially efficient to reduce the deterioration of streaming video transferred on the subcarriers of low power strength without any modifications of the existing schedulers, packet ordering/reassembly, and subcarrier allocation strategies in the base station.

• Journal of the Korea Society for Simulation
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• v.20 no.3
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• pp.119-127
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• 2011

Overhaul and repair service means the sequential processes of disassembly, repair and reassembly for a product which has been used for a long time. Overhaul is required for the companies producing airplanes, ships, trains, military weapons and heavy industrial equipments which are very expensive and have a long life cycle. The most important performance measure of the overhaul repair shop is usually the lead time. Thus, how to design the manufacturing system to meet the delivery date is a major concern in overhaul repair shop. This paper introduces the case study of an overhaul repair shop producing military weapon systems with the 3D simulation tool, $QUEST^{TM}$. At first, the characteristics of overhaul shop and what should be considered for simulation modeling are explained. Then, various simulation scenarios including two types of disassembly systems, one is flow line system and the other is cell system, are discussed with the results of simulation experiments.

• Journal of the Korean Society of Propulsion Engineers
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• v.25 no.3
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• pp.62-80
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• 2021

The requirements for the next-generation propulsion system and for a good propellant have been summarized. The characteristics and effectiveness of kerosene, hydrogen, and methane, which are the fuels that are mainly attracting attention in Korea and abroad, were compared with each other. As a result of the comparison, methane was evaluated to be more advantageous than other fuels in reliability, cost, reusability, maintenance, eco-friendliness, safety, lifespan, technical difficulties, engine cycle selection, application of common bulkhead, and non-disassembly/reassembly delivery. And in terms of performance, the specific impulse of methane is higher than that of kerosene, so the efficiency of the launch vehicle can be increased. Methane's properties incluidng eco-friendliness, low-temperature combustion, long life, and maintenability make it beneficial for reuse and for the development of multi-purpose engines.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.2430-2434
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• 2005

Automatic FPC punching instrument for the improvement of working condition and cost saving is introduced in this paper. FPC(flexible printed circuit) is used to detect the contact position of K/B and button like a cellular phone. Depending on the quality of the printed ink and position of reference punching point to the FPC, the resistance and current are varied to the malfunctioning values. The size of reference punching point is 2mm and the above. Because the punching operation is done manually, the accuracy of the punching degree is varied with operator's condition. Recently, The punching accuracy has deteriorated severely to the 2mm punching reference hall so that assembly of the K/B has hardly done. To improve this manual punching operation to the FPC, automatic FPC punching system is introduced. Precise mechanical parts like a 5-step stepping motor and ball screw mechanism are designed and tested and low cost PC camera is used for the sake of cost down instead of using high quality vision systems for the FA. 3D Mechanical design tool(Pro/E) is used to manage the exact tolerance circumstances and avoid design failures. Simulation is performed to make the complete vision based punching machine before assembly, and this procedure led to the manufacturing cost saving. As the image processing algorithms, dilation, erosion, and threshold calculation is applied to obtain an exact center position from the FPC print marks. These image processing algorithms made the original images having various noises have clean binary pixels which is easy to calculate the center position of print marks. Moment and Least square method are used to calculate the center position of objects. In this development circumstance, Moment method was superior to the Least square one at the calculation of speed and against noise. Main control panel is programmed by Visual C++ and graphical Active X for the whole management of vision based automatic punching machine. Operating modes like manual, calibration, and automatic mode are added to the main control panel for the compensation of bad FPC print conditions and mechanical tolerance occurring in the case of punch and die reassembly. Test algorithms and programs showed good results to the designed automatic punching system and led to the increase of productivity and huge cost down to law material like FPC by avoiding bad quality.

• The Korean Journal of Nuclear Medicine Technology
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• v.12 no.1
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• pp.44-48
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• 2008

Purpose: In FDG-PET/CT of breast cancer, a sensitivity was 80~96% and a specificity was 75~95% commonly. It was valuable to identify a cancer in early stage been difficult in Mammography. Most of the PET/CT scans have been examined on supine position, so, the image of breast has been acquired by reconstructed whole body scan image. However, using prone position with a compensator, a shape of breast was reassembly shown to be real by gravity. Therefore, the purpose of this study was to evaluate diagnostic value of prone position in FDG PET-CT of breast cancer. Materials and Methods: 30 female patients with doubtful or positive breast cancer were examined. The PET-CT whole body scan was acquired at 60 minutes after $^{18}F$-FDG injection on Supine position. Then, regional breast spot scan was progressed on prone position using a compensator. Each image was evaluated by physicians blinded to patient's data, and statistical analysis did through SUVs measured in PET-CT images. Results: In 27 of 30 patients, prone position was shown accurate discrimination and diagnostic value, but in another 3 patients had a lesion 1cm below, PET-CT couldn't detect it, unlike MRI. Consequently, prone position distinguished a lesion better than Supine position, because of low degree of metamorphosis by gravity. The SUVs analysis of each position was significant (p value=0.004). Conclusion: In PET-CT of breast cancer, prone position could detect micrometastasis as well as primary lesion, better than supine position. Therefore, this study proposes that any technical change considered morphological feature like prone position can offer adequate and useful diagnostic information, together with complementary quantitative analysis.