• Title/Summary/Keyword: Real-time link

Search Result 445, Processing Time 0.027 seconds

Molecular Target Therapy of AKT and NF-kB Signaling Pathways and Multidrug Resistance by Specific Cell Penetrating Inhibitor Peptides in HL-60 Cells

  • Davoudi, Zahra;Akbarzadeh, Abolfazl;Rahmatiyamchi, Mohammad;Movassaghpour, Ali Akbar;Alipour, Mohsen;Nejati-Koshki, Kazem;Sadeghi, Zohre;Dariushnejad, Hassan;Zarghami, Nosratollah
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.15 no.10
    • /
    • pp.4353-4358
    • /
    • 2014
  • Background: PI3/AKT and NF-kB signaling pathways are constitutively active in acute myeloid leukemia and cross-talk between the two has been shown in various cancers. However, their role in acute myeloid leukemia has not been completely explored. We therefore used cell penetrating inhibitor peptides to define the contributions of AKT and NF-kB to survival and multi drug resistance (MDR) in HL-60 cells. Materials and Methods: Inhibition of AKT and NF-kB activity by AKT inhibitor peptide and NBD inhibitor peptide, respectively, resulted in decreased expression of mRNA for the MDR1 gene as assessed by real time PCR. In addition, treatment of HL-60 cells with AKT and NBD inhibitor peptides led to inhibition of cell viability and induction of apoptosis in a dose dependent manner as detected by flow cytometer. Results: Finally, co-treatment of HL-60 cells with sub-optimal doses of AKT and NBD inhibitor peptides led to synergistic apoptotic responses in AML cells. Conclusions: These data support a strong biological link between NF-kB and PI3-kinase/AKT pathways in the modulation of antiapoptotic and multi drug resistant effects in AML cells. Synergistic targeting of these pathways using NF-kB and PI3-kinase/AK inhibitor peptides may have a therapeutic potential for AML and possibly other malignancies with constitutive activation of these pathways.

Handover Scheme between WiFi and Mobile WiMax (WiFi와 mobile WiMax간 핸드오버 방안)

  • Park, Seung-Kyun
    • The Journal of the Korea Contents Association
    • /
    • v.11 no.1
    • /
    • pp.34-41
    • /
    • 2011
  • At present wireless internet access service is available through the 3G network, mobile WiMAX and WiFi anytime and anywhere. In this environment where there are various networks, users should be able to select specific networks depending on different situations. And it is necessary to provide mobility support between homogeneous and between heterogenous networks. Given this situation, the many proposals have been presented to link 3G, which has the largest service area among various networks, with mobile WiMAX(IEEE 802.16e), or with WiFi(IEEE 802.11). But, recently, with the increasing volume of wireless internet use and wireless internet data, due to the advents of net-book, e-book and smart phone, the service area of WiFi and mobile WiMAX has rapidly expanded. Especially, the availability of real-time application such as internet phone has led to the relative shrinking of the proportion of 3G mobile communication network giving conventional voice service, and enlargement of those of wireless internet access networks like WiFi and mobile WiMAX. This paper suggests a handover scheme based on PMIPv6, whitch support mobility between WiFi and mobile WiMAX, and minimizes handover delay. In this scheme, the mobile node has a dual stack structure composed of two interfaces-WiFi and mobile WiMAX. Since WiFi dose not support mobility, it is suggested that the mobile node have the capacity to deal with handover signaling between gateway in case of handover between homogeneous networks. This handover scheme, suggested comparing with current handovers between homogeneous networks, has proved, in its analytic evaluation, to be able to reduce handover, transmission, and signaling overhead.

Implementation of Interactive Media Content Production Framework based on Gesture Recognition (제스처 인식 기반의 인터랙티브 미디어 콘텐츠 제작 프레임워크 구현)

  • Koh, You-jin;Kim, Tae-Won;Kim, Yong-Goo;Choi, Yoo-Joo
    • Journal of Broadcast Engineering
    • /
    • v.25 no.4
    • /
    • pp.545-559
    • /
    • 2020
  • In this paper, we propose a content creation framework that enables users without programming experience to easily create interactive media content that responds to user gestures. In the proposed framework, users define the gestures they use and the media effects that respond to them by numbers, and link them in a text-based configuration file. In the proposed framework, the interactive media content that responds to the user's gesture is linked with the dynamic projection mapping module to track the user's location and project the media effects onto the user. To reduce the processing speed and memory burden of the gesture recognition, the user's movement is expressed as a gray scale motion history image. We designed a convolutional neural network model for gesture recognition using motion history images as input data. The number of network layers and hyperparameters of the convolutional neural network model were determined through experiments that recognize five gestures, and applied to the proposed framework. In the gesture recognition experiment, we obtained a recognition accuracy of 97.96% and a processing speed of 12.04 FPS. In the experiment connected with the three media effects, we confirmed that the intended media effect was appropriately displayed in real-time according to the user's gesture.

Macroscopic-Microscopic Sequential Traffic Simulation Analysis and Dynamic O/D Estimation for Sub-area (거시-미시 순차적 교통시뮬레이션 방법과 부분상세지구의 동적 O/D추정)

  • Lee, Jin Hak;Kim, Ikki;Kim, Dae Hyun
    • Journal of Korean Society of Transportation
    • /
    • v.32 no.6
    • /
    • pp.567-578
    • /
    • 2014
  • The study suggested a method to improve analysis accuracy such that the interactive effects of transportation changes between outside and inside of sub-areas were sequentially considered in the analysis by linking a macroscopic network analysis and a microscopic traffic simulation. A dynamic O/D estimation method was developed for practical implement of sub-area microscopic simulation analysis by using the results of macroscopic network analysis, the results of selected link analysis at the cordon line of the sub-area, departure time data of household travel survey, timely observed traffic volume data at the cordon. This estimated dynamic O/D for the sub-area made it possible to analyze traffic phenomena in details. Various detailed phenomena such as traffic queues, delay at intersection, and conflicts between vehicles, which is impossible to be grasped through a macroscopic analysis, can be analyzed with the dynamic microscopic traffic analysis. Through implementing an empirical study and validation, the study provided a reference result about accuracy of a microscopic traffic simulation of a sub-area to help its application for real transportation policy analysis.

Short-Term Prediction of Vehicle Speed on Main City Roads using the k-Nearest Neighbor Algorithm (k-Nearest Neighbor 알고리즘을 이용한 도심 내 주요 도로 구간의 교통속도 단기 예측 방법)

  • Rasyidi, Mohammad Arif;Kim, Jeongmin;Ryu, Kwang Ryel
    • Journal of Intelligence and Information Systems
    • /
    • v.20 no.1
    • /
    • pp.121-131
    • /
    • 2014
  • Traffic speed is an important measure in transportation. It can be employed for various purposes, including traffic congestion detection, travel time estimation, and road design. Consequently, accurate speed prediction is essential in the development of intelligent transportation systems. In this paper, we present an analysis and speed prediction of a certain road section in Busan, South Korea. In previous works, only historical data of the target link are used for prediction. Here, we extract features from real traffic data by considering the neighboring links. After obtaining the candidate features, linear regression, model tree, and k-nearest neighbor (k-NN) are employed for both feature selection and speed prediction. The experiment results show that k-NN outperforms model tree and linear regression for the given dataset. Compared to the other predictors, k-NN significantly reduces the error measures that we use, including mean absolute percentage error (MAPE) and root mean square error (RMSE).

Energy and Delay-Efficient Multipath Routing Protocol for Supporting Mobile Sink in Wireless Sensor Networks (무선 센서 네트워크에서 이동 싱크를 지원하기 위한 다중 경로 라우팅 프로토콜)

  • Lee, Hyun Kyu;Lee, Euisin
    • KIPS Transactions on Computer and Communication Systems
    • /
    • v.5 no.12
    • /
    • pp.447-454
    • /
    • 2016
  • The research on multipath routing has been studied to solve the problem of frequent path breakages due to node and link failures and to enhance data delivery reliability in wireless sensor networks. In the multipath routing, mobile sinks such as soldiers in battle fields and rescuers in disaster areas bring about new challenge for handling their mobility. The sink mobility requests new multipath construction from sources to mobile sinks according to their movement path. Since mobile sinks have continuous mobility, the existing multipath can be exploited to efficiently reconstruct to new positions of mobile sinks. However, the previous protocols do not address this issue. Thus, we proposed an efficient multipath reconstruction protocol called LGMR for mobile sinks in wireless sensor networks. The LGMR address three multipath reconstruction methods based on movement types of mobile sinks: a single hop movement-based local multipath reconstruction, a multiple hop movement-based local multipath reconstruction, and a multiple hop movement-based global multipath reconstruction. Simulation results showed that the LGMR has better performance than the previous protocol in terms of energy consumption and data delivery delay.

Research on rapid source term estimation in nuclear accident emergency decision for pressurized water reactor based on Bayesian network

  • Wu, Guohua;Tong, Jiejuan;Zhang, Liguo;Yuan, Diping;Xiao, Yiqing
    • Nuclear Engineering and Technology
    • /
    • v.53 no.8
    • /
    • pp.2534-2546
    • /
    • 2021
  • Nuclear emergency preparedness and response is an essential part to ensure the safety of nuclear power plant (NPP). Key support technologies of nuclear emergency decision-making usually consist of accident diagnosis, source term estimation, accident consequence assessment, and protective action recommendation. Source term estimation is almost the most difficult part among them. For example, bad communication, incomplete information, as well as complicated accident scenario make it hard to determine the reactor status and estimate the source term timely in the Fukushima accident. Subsequently, it leads to the hard decision on how to take appropriate emergency response actions. Hence, this paper aims to develop a method for rapid source term estimation to support nuclear emergency decision making in pressurized water reactor NPP. The method aims to make our knowledge on NPP provide better support nuclear emergency. Firstly, this paper studies how to build a Bayesian network model for the NPP based on professional knowledge and engineering knowledge. This paper presents a method transforming the PRA model (event trees and fault trees) into a corresponding Bayesian network model. To solve the problem that some physical phenomena which are modeled as pivotal events in level 2 PRA, cannot find sensors associated directly with their occurrence, a weighted assignment approach based on expert assessment is proposed in this paper. Secondly, the monitoring data of NPP are provided to the Bayesian network model, the real-time status of pivotal events and initiating events can be determined based on the junction tree algorithm. Thirdly, since PRA knowledge can link the accident sequences to the possible release categories, the proposed method is capable to find the most likely release category for the candidate accidents scenarios, namely the source term. The probabilities of possible accident sequences and the source term are calculated. Finally, the prototype software is checked against several sets of accident scenario data which are generated by the simulator of AP1000-NPP, including large loss of coolant accident, loss of main feedwater, main steam line break, and steam generator tube rupture. The results show that the proposed method for rapid source term estimation under nuclear emergency decision making is promising.

Walnut phenolic extracts reduce telomere length and telomerase activity in a colon cancer stem cell model

  • Shin, Phil-Kyung;Zoh, Yoonchae;Choi, Jina;Kim, Myung-Sunny;Kim, Yuri;Choi, Sang-Woon
    • Nutrition Research and Practice
    • /
    • v.13 no.1
    • /
    • pp.58-63
    • /
    • 2019
  • BACKGROUND/OBJECTIVES: Telomeres are located at the chromosomal ends and progressively shortened during each cell cycle. Telomerase, which is regulated by hTERT and c-MYC, maintains telomeric DNA sequences. Especially, telomerase is active in cancer and stem cells to maintain telomere length for replicative immortality. Recently we reported that walnut phenolic extract (WPE) can reduce cell viability in a colon cancer stem cell (CSC) model. We, therefore, investigated the effect of WPE on telomere maintenance in the same model. MATERIALS AND METHODS: $CD133^+CD44^+$ cells from HCT116, a human colon cancer cell line, were sorted by Fluorescence-activated cell sorting (FACS) and treated with WPE at the concentrations of 0, 10, 20, and $40{\mu}g/mL$ for 6 days. Telomere lengths were assessed by quantitative real-time PCR (qRT-PCR) using telomere specific primers and DNA extracted from the cells, which was further adjusted with single-copy gene and reference DNA ($ddC_t$). Telomerase activity was also measured by qRT-PCR after incubating the PCR mixture with cell protein extracts, which was adjusted with reference DNA ($dC_t$). Transcriptions of hTERT and c-MYC were determined using conventional RT-PCR. RESULTS: Telomere length of WPE-treated cells was significantly decreased in a dose-dependent manner ($5.16{\pm}0.13$ at $0{\mu}g/mL$, $4.79{\pm}0.12$ at $10{\mu}g/mL$, $3.24{\pm}0.08$ at $20{\mu}g/mL$ and $3.99{\pm}0.09$ at $40{\mu}g/mL$; P = 0.0276). Telomerase activities concurrently decreased with telomere length ($1.47{\pm}0.04$, $1.09{\pm}0.01$, $0.76{\pm}0.08$, and $0.88{\pm}0.06$; P = 0.0067). There was a positive correlation between telomere length and telomerase activity (r = 0.9090; P < 0.0001). Transcriptions of both hTERT and c-MYC were also significantly decreased in the same manner. CONCLUSION: In the present cell culture model, WPE reduced telomere maintenance, which may provide a mechanistic link to the effect of walnuts on the viability of colon CSCs.

IoT data trust techniques based on auto-encoder through IoT-linked processing (오토인코더 기반의 IoT 연계 처리를 통한 IoT 데이터 신뢰 기법)

  • Yon, Yong-Ho;Jeong, Yoon-Su
    • Journal of Digital Convergence
    • /
    • v.19 no.11
    • /
    • pp.351-357
    • /
    • 2021
  • IoT devices, which are used in various ways in distributed environments, are becoming more important in data transmitted and received from IoT devices as fields of use such as medical, environment, transportation, bio, and public places are diversified. In this paper, as a method to ensure the reliability of IoT data, an autoencoder-based IoT-linked processing technique is proposed to classify and process numerous data by various important attributes. The proposed technique uses correlation indices for each IoT data so that IoT data is grouped and processed by blockchain by characteristics for IoT linkage processing based on autoencoder. The proposed technique expands and operates into a blockchain-based n-layer structure applied to the correlation index to ensure the reliability of IoT data. In addition, the proposed technique can not only select IoT data by applying weights to IoT collection data according to the correlation index of IoT data, but also reduce the cost of verifying the integrity of IoT data in real time. The proposed technique maintains the processing cost of IoT data so that IoT data can be expanded to an n-layer structure.

PRC Maritime Operational Capability and the Task for the ROK Military (중국군의 해양작전능력과 한국군의 과제)

  • Kim, Min-Seok
    • Strategy21
    • /
    • s.33
    • /
    • pp.65-112
    • /
    • 2014
  • Recent trends show that the PRC has stepped aside its "army-centered approach" and placed greater emphasis on its Navy and Air Force for a wider range of operations, thereby reducing its ground force and harnessing its economic power and military technology into naval development. A quantitative growth of the PLA Navy itself is no surprise as this is not a recent phenomenon. Now is the time to pay closer attention to the level of PRC naval force's performance and the extent of its warfighting capacity in the maritime domain. It is also worth asking what China can do with its widening naval power foundation. In short, it is time to delve into several possible scenarios I which the PRC poses a real threat. With this in mind, in Section Two the paper seeks to observe the construction progress of PRC's naval power and its future prospects up to the year 2020, and categorize time frame according to its major force improvement trends. By analyzing qualitative improvements made over time, such as the scale of investment and the number of ships compared to increase in displacement (tonnage), this paper attempts to identify salient features in the construction of naval power. Chapter Three sets out performance evaluation on each type of PRC naval ships as well as capabilities of the Navy, Air Force, the Second Artillery (i.e., strategic missile forces) and satellites that could support maritime warfare. Finall, the concluding chapter estimates the PRC's maritime warfighting capability as anticipated in respective conflict scenarios, and considers its impact on the Korean Peninsula and proposes the directions ROK should steer in response. First of all, since the 1980s the PRC navy has undergone transitions as the focus of its military strategic outlook shifted from ground warfare to maritime warfare, and within 30 years of its effort to construct naval power while greatly reducing the size of its ground forces, the PRC has succeeded in building its naval power next to the U.S.'s in the world in terms of number, with acquisition of an aircraft carrier, Chinese-version of the Aegis, submarines and so on. The PRC also enjoys great potentials to qualitatively develop its forces such as indigenous aircraft carriers, next-generation strategic submarines, next-generation destroyers and so forth, which is possible because the PRC has accumulated its independent production capabilities in the process of its 30-year-long efforts. Secondly, one could argue that ROK still has its chances of coping with the PRC in naval power since, despite its continuous efforts, many estimate that the PRC naval force is roughly ten or more years behind that of superpowers such as the U.S., on areas including radar detection capability, EW capability, C4I and data-link systems, doctrines on force employment as well as tactics, and such gap cannot be easily overcome. The most probable scenarios involving the PRC in sea areas surrounding the Korean Peninsula are: first, upon the outbreak of war in the peninsula, the PRC may pursue military intervention through sea, thereby undermining efforts of the ROK-U.S. combined operations; second, ROK-PRC or PRC-Japan conflicts over maritime jurisdiction or ownership over the Senkaku/Diaoyu islands could inflict damage to ROK territorial sovereignty or economic gains. The PRC would likely attempt to resolve the conflict employing blitzkrieg tactics before U.S. forces arrive on the scene, while at the same time delaying and denying access of the incoming U.S. forces. If this proves unattainable, the PRC could take a course of action adopting "long-term attrition warfare," thus weakening its enemy's sustainability. All in all, thiss paper makes three proposals on how the ROK should respond. First, modern warfare as well as the emergent future warfare demonstrates that the center stage of battle is no longer the domestic territory, but rather further away into the sea and space. In this respect, the ROKN should take advantage of the distinct feature of battle space on the peninsula, which is surrounded by the seas, and obtain capabilities to intercept more than 50 percent of the enemy's ballistic missiles, including those of North Korea. In tandem with this capacity, employment of a large scale of UAV/F Carrier for Kill Chain operations should enhance effectiveness. This is because conditions are more favorable to defend from sea, on matters concerning accuracy rates against enemy targets, minimized threat of friendly damage, and cost effectiveness. Second, to maintain readiness for a North Korean crisis where timely deployment of US forces is not possible, the ROKN ought to obtain capabilities to hold the enemy attack at bay while deterring PRC naval intervention. It is also argued that ROKN should strengthen its power so as to protect national interests in the seas surrounding the peninsula without support from the USN, should ROK-PRC or ROK-Japan conflict arise concerning maritime jurisprudence. Third, the ROK should fortify infrastructures for independent construction of naval power and expand its R&D efforts, and for this purpose, the ROK should make the most of the advantages stemming from the ROK-U.S. alliance inducing active support from the United States. The rationale behind this argument is that while it is strategically effective to rely on alliance or jump on the bandwagon, the ultimate goal is always to acquire an independent response capability as much as possible.