• 생명과학회지
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• 제26권5호
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• pp.620-631
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• 2016

바이로이드는 매우 작은 RNA 분자로 구성되어 있으며, 외피 단백질이 없고 오로지 식물에만 감염되어 질병을 유발한다. 바이로이드 감염 질병을 예방하거나 진단하는 것은 상당히 어려운 일이며, 이는 병징이 초기에는 발견되지 않고 수확기에 접어들어서 발견되기 때문이다. 한편, 혈청학적인 방법은 식물 병원체를 검출하기 위해 주로 사용되었으나 바이로이드는 핵산인 RNA로만 구성되어 있기 때문에 이 방법으로 검출할 수가 없다. 때문에 바이로이드를 검출하기 위해 주로 사용되는 방법은 분자 생물학적인 방법으로, 초기에는 바이로이드의 분자적인 크기와 구조적 특징을 이용한 겔 전기 영동 방법이 주로 사용되었다. 그 후에는 역전사 반응과 중합효소 연쇄반응을 접목시킨 역전사 중합효소 연쇄반응(RT-PCR) 방법이 활용되었고, 그에 대한 효율적인 결과 확인을 위해 형광 물질을 도입한 실시간 역전사 중합효소 연쇄반응(Real-time RT-PCR)이 도입되었다. 그러나 그들은 온도를 변화시키기 위한 값비싼 기기와 전문적인 인력이 필요함으로 현장에서는 활용되기가 어렵다. 최근 개발된 고리 기반의 등온 증폭법(Loop-mediated isothermal amplification)의 경우, 온도의 변화가 필요 없어 비싼 온도 조절 기기가 필요하지 않다. 또한 매우 높은 증폭 효율을 지니며 반응 시간이 짧은 등의 여러 장점을 지니고 있기에 최근 현장 진단용 기술에 도입되고 있다. 이러한 배경으로, 이 총설에서는 바이로이드 유발 질병에 대하여 요약하고 그에 대한 검출 및 진단 방법에 대한 연구 동향에 대하여 기술하였다.

• 한국미생물·생명공학회지
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• 제46권2호
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• pp.171-179
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• 2018

Human papillomavirus (HPV) plays a critical role in the development of cervical carcinoma. This study analyzed the efficiency of multiplex real-time PCR in detecting and identifying HPV genotypes in samples from women who visited a Korean hospital for checkups. Cervical swab specimens were obtained from women who attended a checkup at the Health Improvement Center of Hospital in Dankook University Cheonan, South Korea and were referred for an HPV genotyping test between January and September 2014. A total of 1703 cervical swab specimens were collected consecutively during this period. PCR results were compared with those of the traditional cytological assay for the same population. Among the 1,703 specimens, 19.91% were HPV positive, of which 14.50% indicated a single infection and 5.40% indicated multiple infections. However, cytology identified only 2.52% of positive cases, including 1.23% cases of atypical squamous cells of undetermined significance, 1% of low grade squamous intra-epithelial lesion, and 0.29% of high grade squamous intra-epithelial lesion. The rate of high-risk and low-risk HPV in the abnormal cytology group was 48 and 23, respectively, and 274 and 136 in the normal group, respectively. HPV types 56, 52, 43 were the most prevalent in that order. Our results confirm the efficiency of the HPV DNA assay for the detection of 28 different HPV genotypes with reasonable sensitivity. A screening strategy that comprises the HPV DNA assay and cytology would help overcome the low sensitivity of a cytological diagnosis.

• The Plant Pathology Journal
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• 제38권6호
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• pp.656-664
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• 2022

Pectobacterium odoriferum is the primary causative agent in Kimchi cabbage soft-rot diseases. The pathogenic bacteria Pectobacterium genera are responsible for significant yield losses in crops. However, P. odoriferum shares a vast range of hosts with P. carotovorum, P. versatile, and P. brasiliense, and has similar biochemical, phenotypic, and genetic characteristics to these species. Therefore, it is essential to develop a P. odoriferumspecific diagnostic method for soft-rot disease because of the complicated diagnostic process and management as described above. Therefore, in this study, to select P. odoriferum-specific genes, species-specific genes were selected using the data of the P. odoriferum JK2.1 whole genome and similar bacterial species registered with NCBI. Thereafter, the specificity of the selected gene was tested through blast analysis. We identified novel species-specific genes to detect and quantify targeted P. odoriferum and designed specific primer sets targeting HAD family hydrolases. It was confirmed that the selected primer set formed a specific amplicon of 360 bp only in the DNA of P. odoriferum using 29 Pectobacterium species and related species. Furthermore, the population density of P. odoriferum can be estimated without genomic DNA extraction through SYBR Green-based real-time quantitative PCR using a primer set in plants. As a result, the newly developed diagnostic method enables rapid and accurate diagnosis and continuous monitoring of soft-rot disease in Kimchi cabbage without additional procedures from the plant tissue.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
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• pp.3073-3078
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• 2011

Driverless Train Control System has been recently introduced and commercialized in Korea. It is expected that the vehicle with driverless operation system will be used in new lines such as Sinbundang line soon. Therefore it is necessary to change the system operation and conception of the existing train operation system and the necessity of driverless vehicle monitoring system meeting a new paradigm is rising. In order to dispel concerning about safety issues caused by driverless train operation, the importance to establish vehicle error detection, useful fault diagnosis and rapid action plans is higher than ever. For this, efficient and higher level of vehicle supervision & control system should be essentially supported. In this study, remote driverless vehicle monitoring system using by radio communication is suggested to be used for monitoring and controlling important parts of the vehicle and diagnose and take quick actions when vehicles are in trouble at control tower at real time.

• 대한전자공학회논문지
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• 제24권1호
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• pp.28-34
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• 1987

As a means of improving the reliability of a process control system, a FTCS(Fault Tolerant Control System) is designed and applied to the boiler controller of a thermal power plant. The proposed FTCS has capabilities of fault detection and diagnosis as well as back-up control and bumpless switching. A prototype of FTCS is implemented on an IBM PC as an add-on system and it is experimentally verified by using a boiler process simulator together with simplified analog controllers and a switching unit that an one-fold fault is detected in real time and back-up controller takes over the role of the original controller, controlling the faulty loop.

• 스마트미디어저널
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• 제12권10호
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• pp.71-84
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• 2023

넙치 양식은 우리나라 양식 산업의 절반 이상 차지할 정도로 큰 비중을 차지한다. 그러나 연중 총사육량의 25~30% 정도가 질병으로 인한 집단 폐사가 발생하여 양식장의 경제성에 매우 나쁜 영향을 준다. 넙치 양식장의 경제성 성장을 위해서는 넙치 질병 증상 진단을 자동화하여 빠르고 정확하게 질병 진단을 하는 방법이 필요하다. 본 연구에서는 독창적인 학습 데이터 수집 방법과 학습 정제 알고리즘 및 학습 데이터 분리 기법을 사용하여 학습 데이터를 구축하고 4가지 객체 탐지 딥러닝 모델(YOLOv8, Swin, Vitdet, MvitV2)의 넙치 질병증상 감지 성능을 비교한다. 실험 결과 YOLOv8 모델이 평균 인식률(mAP)과 예상 도착 시간(ETA) 관점에서 우수하다는 결론을 얻었다. 본 연구에서 제안하는 AI 모델의 성능이 검증되면 넙치 양식장에서는 실시간으로 넙치 질병을 진단할 수 있고, 진단 결과에 따른 신속한 예방 조치로 양식장의 생산성은 크게 향상될 것이라 기대된다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 제38회 하계학술대회
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• pp.1558-1559
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• 2007

This study is an application field by using optical fiber. The system to measure sBs from optical fiber is designed, So it can be used as a temperature sensor. by using frequency shift, resulting from temperature changes. the frequency shift is checked by changing temperature from $25^{\circ}C$ to $69^{\circ}C$ with chamber in the laboratory and using 40Km optical fiber. It's also measured by varying the length of optical fiber The program to do the real-time monitoring and analyze the measured data is created to find accurate frequency. It can be used as an optical fiber sensor, which is capable of measuring temperature and distance by using sBs.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1987년도 전기.전자공학 학술대회 논문집(II)
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• pp.1338-1340
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• 1987

This paper describes the design of ECG data preprocess module for the ECG signals. This module process the data obtained from two channels. It is composed of the AID converter, QRS detector, one chip micro-computer and memory. This module performs the following functions;digital filtering, R wave detection and determination of reference point for the ST segment. The measured points are transfered to the next data module by the interrupt process. This preprocessor data module is available to the basis for the parallel data processing for the real time automatic diagnosis.

• Journal of Microbiology and Biotechnology
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• 제24권7호
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• pp.1004-1007
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• 2014

Culture is the gold standard for diagnosis of tuberculosis, but it takes 6 to 8 weeks to confirm the result. This issue is complemented by the detection method using polymerase chain reaction, which is now widely used in a routine microbiology laboratory. In this study, we evaluated the performance of the Seegene Anyplex TB PCR to assess its diagnostic sensitivity and specificity, and compared its results with the Roche Cobas TaqMan MTB PCR, one of the most widely used assays in the world. Five university hospitals located in the Chungcheong area in South Korea participated in the study. A total of 1,167 respiratory specimens ordered for acid-fast bacilli staining and culture were collected for four months, analyzed via the Seegene Anyplex TB PCR, and its results were compared with the Roche Cobas TaqMan MTB PCR. For detection of Mycobacterium tuberculosis, the diagnostic sensitivity and specificity of the Anyplex TB PCR were 87.5% and 98.2% respectively, whereas those of the Cobas TaqMan were 92.0% and 98.0% respectively (p value > 0.05). For smear-positive specimens, the sensitivity of the Anyplex TB PCR was 95.2%, which was exactly the same as that of the Cobas TaqMan. For smear-negative specimens, the sensitivity of the Anyplex TB PCR was 69.2%, whereas that of the Cobas TaqMan TB PCR was 84.6%. For detection of MTB, the Seegene Anyplex TB PCR showed excellent diagnostic performance, and high sensitivity and specificity, which were comparable to the Roche Cobas TaqMan MTB PCR. In conclusion, the Anyplex TB PCR can be a useful diagnostic tool for the early detection of tuberculosis in clinical laboratories.

• Parasites, Hosts and Diseases
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• 제23권2호
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• pp.285-292
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• 1985

The present study has been designed as a basic study on laboratory diagnosis of giardiasis and to demonstrate a more effective method for the detection of Giardia lamblia cyst with the inherent advantages of minimizing both the number of stool examinations required and the interval of stool collections for estimating the real state of prevalence in the shortest time possible. There were 3 subject groups of 75 children each currently residing in an orphanage in Gunsan city, Jeonbuk province from which stool specimens were collected every day, every other day, and every 3 days. The procedure is as follows: 1) resuspend the InKed sample after fixation with SAF solution 2) centrifuge the sediment for 1 min. at 2, 000 rpm after straining through gauze into a tube 3) divide the sediment into 3 parts and use them for direct fecal smear, formalin-ether concentration (MGL) and zinc sulfate ($ZnSO_4$) floatation techniques. The results are summarized as follows: 1. Overall infection rate after 10 trials showed a 60% positive indication. The positive rate among children under 4 years old was significantly higher than the rate in children over 4 years old. No significant difference in rate by sex was observed. 2. The results of eBaminations by direct fecal smear and MGL techniques appeared more accurate than that obtained by $ZnSO_4$ ftoatation method as indicated by a higher positive rate. Of all three methods concerned, combinations of two demonstrated a higher positive rate than that shown by any one alone. 3. In three consecutive examinations under varying conditions such as different days, the cyst detection rate by MGL technique indicated 83%. In 5 examinations under the same varying conditions, the indicated rate was 94%. 4. The interval of stool collection proved to be insignificant for the cyst detection rate. In conclusion, both MGL method and modified fecal direct smear can provide a good cyst detection rate of G. lamblia provided that more than 3 consecutive examinations of stool under varying conditions are carried out.