• Journal of Korean Society on Water Environment
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• v.34 no.1
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• pp.46-56
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• 2018

A mcyB-specific Ultra-Rapid quantitative PCR was developed for the quantitative detection of Microcystis aeruginosa, which is often a dominant species in green tide. McyB-specific UR-qPCR was optimized under extremely short times of each step in thermal cycles, based on the specific primers deduced from the mcyB in microcystin synthetase of M. aeruginosa. The M. aeruginosa strain KG07 was used as a standard for quantification, after the microscopic counting and calculation by mcyB-specific UR-qPCR. The water samples from the river water with the Microcystis outbreak were also measured by using both methods. The $1.0{\times}10^8$ molecules of mcyB-specific DNA was recognized inner 4 minutes after beginning of UR-qPCR, while $1.0{\times}10^4$ molecules of mcyB-specific templates was detected inner 7 minutes with quantitative manner. From the range of $1.0{\times}10^2$ to $1.0{\times}10^8$ initial molecules, quantification was well established based on $C_T$ using mcyB-specific UR-qPCR (Regression coefficiency, $R^2=0.9977$). Between the numbers of M. aeruginosa cell counting under microscope and calculated numbers using mcyB-specific UR-qPCR, some differences were often found. The reasons for these differences were discussed; therefore, easy compensation method was proposed that was dependent on the numbers of the cell counting. Additionally, to easily extract the genomic DNA (gDNA) from the samples, a freeze-fracturing of water-sample using liquid nitrogen was tested, by excluding the conventional gDNA extraction method. It was also verified that there were no significant differences using the UR-qPCR with both gDNAs. In conclusion, the mcyB-specific UR-qPCR that we proposed would be expected to be a useful tool for rapid quantification and easy monitoring of M. aeruginosa in environmental water.

• International Journal of Highway Engineering
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• v.10 no.2
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• pp.159-166
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• 2008

Video image processing systems (VIPS) offer numerous benefits to transportation models and applications, due to their ability to monitor traffic in real time. VIPS based on a wide-area detection algorithm provide traffic parameters such as flow and velocity as well as occupancy and density. However, most current commercial VIPS utilize a tripwire detection algorithm that examines image intensity changes in the detection regions to indicate vehicle presence and passage, i.e., they do not identify individual vehicles as unique targets. If VIPS are developed to track individual vehicles and thus trace vehicle trajectories, many existing transportation models will benefit from more detailed information of individual vehicles. Furthermore, additional information obtained from the vehicle trajectories will improve incident detection by identifying lane change maneuvers and acceleration/deceleration patterns. However, unlike human vision, VIPS cameras have difficulty in recognizing vehicle movements over a detection zone longer than 100 meters. Over such a distance, the camera operators need to zoom in to recognize objects. As a result, vehicle tracking with a single camera is limited to detection zones under 100m. This paper develops a methodology capable of monitoring individual vehicle trajectories based on image processing. To improve traffic flow surveillance, a long distance tracking algorithm for use over 200m is developed with multi-closed circuit television (CCTV) cameras. The algorithm is capable of recognizing individual vehicle maneuvers and increasing the effectiveness of incident detection.

• Journal of the Korea Convergence Society
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• v.4 no.2
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• pp.29-33
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• 2013

From one elementary school of last 2005 Pusan was started "the school police" system is being applied the effect in the elementary school of the human nature receiving entire country. But "with school police system " have a limit about manpower that is insufficient. So we try to make "Baumter-Gikimy " that is extended scollo police in seoul. Despite being like that at 2010 and 2011 cannot forget the elementary student suicide events which are shocking or sexual assault events occur from the campus.From elementary school 4 grade the students between junior high school 1 grade crime occurrence ratio are highest. These people the group leaving out mainly, suicide, with group violence and sexual assault problem gets up same problems. In order to solve like this problems stands the countermeasure of the dimension which is national certainly is necessary. But so far only the damage student and the parents have an interest in about like this problems and they hurt. We the smart phone of Android bases it will be able to solve like this problem points [ayp] from under planning and development boil the elementary student safe helper it will can use. When it uses the elementary student safe helper system which we propose before the accident occurs, it will grasp the state information of the student and it will be able to prevent an accident in advance. We will try to more efficient safe guard to elementary school student by real-time monitoring skill soon.

• Journal of the Korean Institute of Intelligent Systems
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• v.25 no.3
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• pp.279-286
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• 2015

The rapid increase of the current marine accidents is mainly due to the human execution errors. In an effort to address this, various kinds of researches such as construction of the digital vessels and vessel information monitoring systems have been conducted. But for safe navigation of vessels, it lack on systems study which can efficiently store, utilize and manage the mass data accepted by the vessel. In this paper, we propose a VWS(Virtual World System) that is based on the architecture of intelligent systems RVC(Reactive Layer-Virtual World-Considerative Layer) model of intelligent autonomous navigation system. VWS is responsible to store all the necessary information for safe navigation of the vessel and the information services to the sub-system of intelligent autonomous navigation system. VWS uses topology database to express the specific problem area, and utilizes a scheduling to reflect the characteristics of the real-time processing environment. Also, Virtual World defines API for the system to reflect the characteristics of the distributed processing environment. As a case study, the VWS is applied to a intelligent ship autonomous navigation system, and simulation is done to prove the effectiveness of the proposed system.

• Journal of Internet Computing and Services
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• v.13 no.1
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• pp.37-44
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• 2012

Numerous scientists who are engaged in compute-intensive researches require more computing facilities than before, while the computing resource and techniques are increasingly becoming more advanced. For this reason, many works for e-Science environment have been actively invested and established around the world, but still the scientists look for an intuitive experimental environment, which is guaranteed the improved environmental facilities without additional configurations or installations. In this paper, we present an integrated scientific workflow execution environment for Scientific applications supporting workflow design with high performance computing infrastructure and accessibility for web browser. This portal supports automated consecutive execution of computation jobs in order of the form defined by workflow design tool and execution service concerning characteristics of each job to batch over distributed grid resources. Workflow editor of the portal presents a high-level frontend and easy-to-use interface with monitoring service, which shows the status of workflow execution in real time so that user can check the intermediate data during experiments. Therefore, the scientists can take advantages of the environment to improve the productivity of study based on HTC.

• Journal of Life Science
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• v.28 no.6
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• pp.639-647
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• 2018

A new heat shock protein 70 was identified in red-spotted grouper (Epinephelus akaara) based on an expression analysis. The cDNA of red-spotted grouper Hsp70 (designated RgHsp70) was cloned by the rapid amplification of cDNA ends (RACE) techniques. The full-length of RgHsp70 cDNA was 2,152 bp, consisting of a 5'-terminal untranslated region (UTR) of 105 bp, a 3'-terminal UTR of 274 bp, and an open reading frame (ORF) of 1,773 bp that encode a polypeptide of 590 amino acids with a theoretical molecular weight of 64.9 kDa and an estimated isoelectric point of 5.2. Multiple alignment and phylogenetic analyses revealed that the RgHsp70 gene shares a high similarity with other Hsp70 fish genes. RgHsp70 contained all three classical Hsp70 family signatures. The results indicated the RgHsp70 is a member of the heat shock protein 70 family. RgHsp70 mRNA was predominately expressed in the liver, with reduced expression noted in the head-kidney tissues. The expression analysis of different water temperatures (21, 18, 15 and $12^{\circ}C$) for sampled livers revealed that expression gradually increased at $12^{\circ}C$ compared to $21^{\circ}C$. In this study, the effects of water temperature lowering on the physiological conditions were investigated, and the results revealed that novel RgHsp70 may be an important molecule involved in stress responses.

• The Journal of the Korea institute of electronic communication sciences
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• v.8 no.8
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• pp.1247-1255
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• 2013

In this paper, We proposes multipurpose function electronic measurement reference station prototype system based on LED and Gyro Sensor. This system have developed to possible with real time data collection and landscape illumination and surrounding diastrophism monitoring for that analyze problems of existing planted reference station. Hardware for the proposed system consists of a gyro sensor module to detect diastrophism, GPS module to process location information, environment sensor module to process surrounding environmental data and CDMA wireless data communication to send the collected data to server. In addition, this paper intends to enhance system management and future usability in a way that applies LED, QR-code, RFID and Solar cell module to outer side of electronic measurement reference station to improve usability of H/W for electronic measurement reference station. Lastly, this paper conducted current amount evaluation to supply stabilized electricity with its various functions in the proposed multipurpose function electronic measurement reference station and it's proved that this system can be stably operated with its electricity loss factor of 2.29% loss factor. And this paper conducted a qualitative comparison with existing electronic measurement reference station system in order to evaluate superiority of the proposed electronic measurement reference station.

• Journal of Korea Multimedia Society
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• v.14 no.6
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• pp.794-801
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• 2011

Main machinery in the factory have various digital indicator to display its operating data for the manager. It is useful to control these datum at the remote office for factory automation in order to increase machine productivity. It is easy to use Ethernet infrastructure for remote communication. But most of all machinery in domestic factories still support RS232c or RS485 serial communication way. In this case to control machinery datum on the ethernet base, it is need to change RS232C or RS485 data to ethernet base. In this paper, in order to develop a remote progress management system through the wireless Internet, we show a design method to make easy maintenance by developing the system with both the JAVA language and RxTx Package for RS232c serial communication. For the system implementation, we added the digital indicator connect with a thermo couple to a machine which provide the real time status data of temperature. To connect machines management server program, we set up a convertor to change the RS485 data to the RS232c and also another convertor to change the RS232c data to wireless ethernet.

• The KIPS Transactions:PartA
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• v.10A no.6
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• pp.691-700
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• 2003

Due to the fast development of wire&wireless internet and computer hardware, more and more internet services are being developed, such as Internet broadcast, VoD (Video On Demand), etc. So QoS (Qualify of Service) is essentially needed to guarantee the quality of these services. Traditional Internet is Best-Effort service in which all packets are transported in FIFO (First In First Out) style. However, FIFO is not suitable to guarantee the quality of some services, so more research in QoS router and QoS protocol are needed. Researched QoS router and protocol are high cost and inefficient because the existing infra is not used. To solve this problem, a new QoS control method, named Network Adaptive QoS, is introduced and applied to client/server-based streaming systems. Based on network bandwidth monitoring mechanism, network adaptive QoS control method can be used in wire&wireless networks to support QoS in real-time streaming system. In order to reduce application cost, the existing streaming service is used in NAQoS. A new module is integrated into the existing server and client. So the router and network line are not changed. By simulation in heavy traffic network conditions, we proved that stream cannot be seamless without network adaptive QoS method.

• Asian Journal of Atmospheric Environment
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• v.11 no.2
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• pp.114-121
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• 2017

Real-time monitoring of hourly concentrations of atmospheric Radon-222 ($^{222}Rn$, radon) and some gaseous pollutants ($SO_2$, CO, $O_3$) was performed throughout 2013-2014 at Gosan station of Jeju Island, one of the cleanest regions in Korea, in order to characterize their background levels and temporal variation trend. The hourly mean concentrations of radon and three gaseous pollutants ($SO_2$, CO, $O_3$) over the study period were $2216{\pm}1100mBq/m^3$, $0.6{\pm}0.7ppb$, $211.6{\pm}102.0ppb$, and $43.0{\pm}17.0ppb$, respectively. The seasonal order of radon concentrations was as fall ($2644mBq/m^3$)$${\sim_\sim}$$winter ($2612mBq/m^3$)>spring ($2022mBq/m^3$)>summer ($1666mBq/m^3$). The concentrations of $SO_2$ and CO showed similar patterns with those of radon as high in winter and low in summer, whereas the $O_3$ concentrations had a bit different trend. Based on cluster analyses of air mass back trajectories, the air mass frequencies originating from Chinese continent, North Pacific Ocean, and the Korean Peninsula routes were 30, 18, and 52%, respectively. When the air masses were moved from Chinese continent to Jeju Island, the concentrations of radon and gaseous pollutants ($SO_2$, CO, $O_3$) were relatively high: $2584mBq/m^3$, 0.76 ppb, 225.8 ppb, and 46.4 ppb. On the other hand, when the air masses were moved from North Pacific Ocean, their concentrations were much low as $1282mBq/m^3$, 0.24 ppb, 166.1 ppb, and 32.5 ppb, respectively.