• 대한본초학회지
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• 제31권6호
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• pp.73-81
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• 2016

Objectives : $18{\beta}$-Glycyrrhetinic acid (18betaGA) is an metabolite of glycyrrhizin in Glycyrrhiza (licorice). The present study investigated anti-inflammatory and anti-apoptosis effect of 18betaGA on the brain tissue of lipopolysaccharide (LPS)-treated C57BL/6 mice. Methods : 18betaGA was administered orally with low (30 mg/kg) and high (100 mg/kg) doses for 3 days prior to LPS (3 mg/kg) injection. Pro-inflammatory cytokines mRNA including tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin (IL)-$1{\beta}$, IL-6, and inflammatory enzyme cyclooxygenase-2 (COX-2) mRNA were measured in the cerebral cortex, hippocampus, and hypothalamus tissue using real-time polymerase chain reaction at 24 h after the LPS injection. Histological changes of Cornu ammonis area 1 (CA1) neurons, Bax, Bcl-2, and caspase-3 expression in the hippocampus was also evaluated by immunohistochemistry and Western blotting method. Results : 18betaGA significantly attenuated the up-regulation of TNF-${\alpha}$, IL-$1{\beta}$, IL-6 mRNA, and COX-2 mRNA expression in the brain tissues induced by the LPS injection. 18betaGA also significantly attenuated the reductions of the thickness of CA1 and the number of CA1 neurons. The up-regulation of Bax protein expression in the hippocampal tissue by the LPS injection was significantly attenuated, while the ratio of Bcl-2/Bax expression was increased by 18betaGA treatment. 18betaGA also significantly attenuated the up-regulation of Bax and caspase-3 expression in the CA1 of the hippocampus. Conclusion : This results indicate that 18betaGA has anti-inflammatory and anti-apoptosis effect under neuroinflammation induced by the LPS injection and suggest that 18betaGA may be a beneficial drug for various brain diseases accompanied with the brain tissue inflammation.

• 대한본초학회지
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• 제28권1호
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• pp.83-90
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• 2013

Objectives : Tetramethylpyrazine (TMP) is an active ingredient in Ligusticum wallichii and has a wide range of neuroprotection effects. This study investigated anti-neuroinflammatory effect of TMP on brain regions in intracerebroventricular (i.c.v.) lipopolysaccharide (LPS)-treated C57BL/6 mice. Methods : TMP was administered intraperitoneally at doses of 10, 20, and 30 mg/kg at 1 h prior to LPS (3 mg/kg) i.c.v. injection. mRNA level of pro-inflammatory cytokines, including tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin (IL)-$1{\beta}$ and IL-6, was measured in the cerebral cortex, hippocampus, and hypothalamus tissue using real-time polymerase chain reaction at 24 h after the LPS injection. Cyclooxygenase-2 (COX-2) positive cells in the hypothalamus was also observed using immunohistochemistry at 24 h after the LPS injection. Results : At a dose of 30 mg/kg TMP significantly attenuated up-regulation of TNF-${\alpha}$ and IL-$1{\beta}$ mRNA in the cerebral cortex and IL-$1{\beta}$ mRNA in the hippocampus. In the hypothalamus, doses of 20 mg/kg and 30 mg/kg TMP significantly attenuated up-regulation of TNF-${\alpha}$, IL-$1{\beta}$, and IL-6 mRNA induced by the LPS injection. In addition, TMP (30 mg/kg) significantly reduced the number of COX-2 positive cells in the hypothalamus. Conclusion : These results indicate that TMP has an anti-inflammatory effect on neuroinflammation, especially in the hypothalamus, induced by LPS i.c.v. injection and suggest that TMP-containing Ligusticum wallichii may play a modulatory role on the systemic responses following hypothalamic inflammation.

• 대한기계학회논문집B
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• 제23권2호
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• pp.159-165
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• 1999

Experimental studies have been performed to observe the basic phenomena of waste bed combustion in MSW incinerator. A reduced scale apparatus was utilized to simulate the combustion behavior in real plant with 1-dimensional transient behavior at the experimental setup, which uses wet cubic wood with ash content as simulated waste. LHV (lower heating value) of solid fuel, fuel particle size and flow rate of combustion air were taken as important parameters of the bed combustion. For the quantitative analysis, FPR (flame propagation rate), TBT (total burn-out time) and PBT (particle burn-out time) was defined. LHV represent the capability of heat release of the fuel, so that a higher LHV results in faster reaction rate of the fuel bed, which is shown by higher FPR. Fuel particle size is related with surface area per unit mass as well as heat and mass transfer coefficient. As the particle size increases the FPR decreases owing to decreasing specific surface area. Air injection supplies oxygen to the reaction zone. However oversupply of combustion air increases convection cooling of the bed and possibly extinguishes the flame.

• 한국정밀공학회지
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• 제25권3호
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• pp.93-100
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• 2008

Laser interferometry is widely used as a measuring system in many fields because of its high resolution and ability to measure a broad area in real-time all at once. In conventional LASER interferometry, for example Out-of-plane ESPI(Electronic Speckle Pattern Interferometry), In plane ESPI, Shearography and Holography, it uses PZT or other components as a phase shift instrumentation to extract 3D deformation data, vibration mode and others. However, in most cases PZT has some disadvantages, which include nonlinear errors and limited time of use. In the present study, a new type of LASER interferometry using a laser diode is proposed. Using LASER Diode Sinusoidal Phase Modulating (LD-SPM) interferometry, the phase modulation can be directly modulated by controlling the LASER Diode injection current thereby eliminating the need for PZT and its components. This makes the interferometry more compact. This paper reports on a new approach to the LD Modulating interferometry that involves four-buckets phase shift method. This study proposes a four-bucket phase mapping algorithm, which was developed to have a guaranteed application, to stabilize the system in the field and to be a user-friendly GUI. In this paper, the theory for LD wavelength modulation and sinusoidal phase modulation of LD modulating interferometry is shown. Four-bucket phase mapping algorithm is then introduced.

• 태양에너지
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• 제7권2호
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• pp.37-53
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• 1987

This study has been prepared for the purpose of developing the system performance prediction method of natural circulation solar hot water system. The storage tank of the natural circulation solar hot water system equipped with flat-plate solar collector is located at higher elevation than the solar collectors. Therefor, the storage tank temperature distribution formed accordance with configuration of storage tank by flow rate of circulating fluid affect system collection efficiency. In this study measure the storage tank temperature distribution with various experimental system under real sun condition and present the theoretical prediction method of the storage tank temperature. Moreover measure the flow rate not only day-time but also night-time reverse flow rate with die injection visual flow meter. Main conclusion obtain from the present study is as follows; 1) The storage tank temperature distribution above the connecting pipe connection position is the same as that of the fully mixed tank and below the connection position is the same as that of stratified tank. 2) The system performance sensitive to the storage tank temperature distribution. Therefore detailed tank model is necessary. Average storage tank temperature can be calculate 3% and storage tank temperature profile can get less than 10% difference with this model system.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 추계학술대회 논문집
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• pp.926-929
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• 2005

Recently, laser interferometer is widely used as a measuring system in many fields because of its high resolution and its ability to measure a board area in real-time ail at once. In conventional laser interferometer, for examples Out of plane ESPI, In plane ESPI, Shearography and Holography, it uses PZT or other components as a phase shift instrumentation to extract 3-D deformation data, vibration mode and others. However, in most cases PZT has some disadvantages, which include noli-linear errors and limited time of use. In present study, a new type of laser interferometer using a laser diode(LD) is proposed. Using Laser Diode Sinusoidal Phase Modulating(LD-SPM) interferometer, the phase modulation can be directly modulated by controlling the LD injection current thereby eliminating the need for PZT components.

• 한국지능시스템학회논문지
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• 제25권4호
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• pp.369-376
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• 2015

정수장 유입수에 포함된 다양한 유기물을 제거하기 위하여 약품을 주입하고 있으나 적정 주입률 결정은 4~7시간 후에나 탁도를 통하여 확인 가능함에 따라 실시간 피드백 제어가 불가하다. 또한 실험실에서 실시하는 Jar-Test 및 운영자의 경험에 따른 수동운전은 유기물 특성 및 수질 변화로 인하여 실험 및 휴먼에러가 발생할 수 있다. 특히 야간/주말 등에는 실험을 실시할 수 없어 운영자 판단에 의한 간헐적 변경만이 이루어지고 있다. 따라서 지능제어 알고리즘을 이용한 적정 약품 주입률을 학습하여 실시간 약품 주입과 주입량 감소로 원가절감을 달성코자 하였다.

• BMB Reports
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• 제39권1호
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• pp.26-36
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• 2006

Differential Display PCR technique (DD-PCR) was used for the analysis of altered gene expression in hemocytes of Vibrio harveyi-infected Penaeus monodon. Forty-four combinations of arbitrary and oligo(dT) primers were used to screen for differentially expressed genes. A total of 79 differentially expressed bands could be identified from 33 primer combinations. These included 48 bands (61%) whose expression level increased and 31 bands (39%) decreased after V. harveyi challenge. Subsequently, forty-eight differential display fragments were successfully reamplified and cloned. A total of 267 clones were randomly selected and sequenced. The sequence analysis showed that 85 (31%) out of 267 clones were matched with sequences in the GenBank database which represented 24 different genes with known functions. Among the known genes, glucose transporter 1, interferon-related developmental regulator 1, lysozyme, profilin, SERPINB3, were selected for further confirmation of their differentially expression patterns by real-time PCR. The results showed increasing in expression level of the selected genes in shrimp hemocytes after microbial challenge suggesting the involvement of such genes in bacterial response in shrimp. The anti-lipopolysaccharide factor type 3 (ALFPm3) gene, previously reported in P. monodon (Supungul et al., 2002) was found among the up-regulated genes but diversity due to amino acid changes was observed. Increase in ALFPm3 transcripts upon V. harveyi injection is in accordance with that found in the previous study.

• 한국수자원학회논문집
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• 제54권9호
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• pp.643-656
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• 2021

최근 국내 상수도 관리 기술은 고도로 발달하고 있으며, 이 과정에서 상수관망 내 용수공급 현황을 파악하고 예측하기 위한 컴퓨터 수리·수질 해석 모형은 핵심적인 역할을 수행하고 있다. 그러나 대규모 네트워크의 경우 컴퓨터 해석모형의 부담을 가중하고, 특히 짧은 계산시간 간격과 긴 모의 시간이 요구되는 수질해석의 경우, 막대한 계산시간이 소요되어 다양한 수질모의 및 분석이 어려운 경우가 발생한다. 본 연구에서는 대규모 상수관망시스템의 수질해석의 계산효율을 개선하기 위해 상수도 공급계통을 2단계로 계층화한 후, 계층화된 네트워크를 대상으로 수질모의를 수행하는 계층적 수질모의 기법을 제안하였다. 제안된 모의기법은 국내 대규모 상수도 네트워크에 적용하였으며, 다양한 염소투입농도 시나리오에 따른 잔류염소농도의 시공간적 분포를 모의하고 분석한 결과를 제시하였다.

• 한국어병학회지
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• 제36권2호
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• pp.229-238
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• 2023

In this study, the correlation between clinical changes and RSIV genome copy number was investigated to determine the quantitative criteria for the characteristics of RSIV infection. The rock bream (Oplegnathus fasciatus) was intraperitoneally injected with three different doses (1.0×10¹, 1.0×10³ and 1.0×10⁵ viral genome copies/fish) as low, medium, and high doses, respectively. The clinical signs (spleen enlargement, death) observation and real-time PCR were conducted at 5, 10 and 14 days post-injection. During the experiment, spleen index as a quantitative indicator for spleen enlargement was continuously increased in the medium- (up to 2.26) and high-dose (up to 4.99) challenge groups, respectively. Notably, when the spleen index was over 1.5, 2.0, 2.5 and 3.0, a positive correlation was revealed with average viral genome copy numbers of 2.51, 3.37, 4.97 and 5.43×10⁷ viral genome copies/mg, respectively. Moreover, the threshold of spleen index over 1.5 was 1.0×10⁶ viral genome copies/mg, while the thresholds of spleen index over 2.0 and dead was 2.51×10⁷ viral genome copies/mg and the thresholds of spleen index over 2.5 and 3 was 3.98×10⁷ viral genome copies/mg. These findings suggest the possibility of quantitatively analyzing the characteristics and development process of RSIV infection.