• Journal of Microbiology and Biotechnology
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• 제22권4호
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• pp.530-534
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• 2012

A real-time PCR assay was developed and validated inhouse specifically for the detection of Clostridium perfringens (Cl. perfringens) in meats and vegetables by comparing with the culture method. The detection limit of the real-time PCR assay in phosphate-buffered saline was $10^2$ CFU/ml. When the two methods were compared in food samples inoculated with Cl. perfringens, the culture method detected 52 positives, whereas real-time PCR detected 51 positives out of 160 samples. The difference was without statistical significance (p>0.05). Real-time PCR assay is an option for quality assurance laboratories to perform standard diagnostic tests, considering its detection ability and time-saving efficiency.

• Journal of Microbiology and Biotechnology
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• 제20권10호
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• pp.1463-1470
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• 2010

E. coli has long been widely used as a host system for the manufacture of recombinant proteins intended for human therapeutic use. When considering the impurities to be eliminated during the downstream process, residual host cell DNA is a major safety concern. The presence of residual E. coli host cell DNA in the final products is typically determined using a conventional slot blot hybridization assay or total DNA Threshold assay. However, both the former and latter methods are time consuming, expensive, and relatively insensitive. This study thus attempted to develop a more sensitive real-time PCR assay for the specific detection of residual E. coli DNA. This novel method was then compared with the slot blot hybridization assay and total DNA Threshold assay in order to determine its effectiveness and overall capabilities. The novel approach involved the selection of a specific primer pair for amplification of the E. coli 16S rRNA gene in an effort to improve sensitivity, whereas the E. coli host cell DNA quantification took place through the use of SYBR Green I. The detection limit of the real-time PCR assay, under these optimized conditions, was calculated to be 0.042 pg genomic DNA, which was much higher than those of both the slot blot hybridization assay and total DNA Threshold assay, where the detection limits were 2.42 and 3.73 pg genomic DNA, respectively. Hence, the real-time PCR assay can be said to be more reproducible, more accurate, and more precise than either the slot blot hybridization assay or total DNA Threshold assay. The real-time PCR assay may thus be a promising new tool for the quantitative detection and clearance validation of residual E. coli host cell DNA during the manufacturingprocess for recombinant therapeutics.

• 한국정보과학회논문지:데이타베이스
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• 제37권6호
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• pp.324-332
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• 2010

실시간 임베디드 센서 네트워크 시스템에서의 이벤트 감지는 대부분 현실세계에서 수집된 센서 데이터들의 조합에 기반한다. 이에 최근에 이루어진 연구들에선 센서 데이터들을 수집, 집계하는 낮은 수준의 다양한 메커니즘들을 제안하였다. 그러나 실시간에서 연속적으로 발생하는 복잡한 이벤트들의 감지와 다양한 종류의 센서들로부터 입력되는 실시간 데이터의 처리를 위한 시스템에 대한 솔루션은 보다 많은 연구를 필요로 한다. 즉, 경량의 데이터 혼합이 가능하고 많은 컴퓨팅 자원을 필요로 하지 않는 실시간 이벤트 감지 기법이 필요하다. 이벤트 감지 프레임워크는 실시간 모니터링과 센서 데이터의 도착으로 일어나는 데이터 융합 메커니즘을 통하여 적시성과 임베디드 센서 네트워크의 자원 요구량을 감소시킬 수 있는 잠재력을 지니고 있다. 또한 임베디드 센서 네트워크 시스템이 신뢰성을 지닐 수 있도록 하기 위한 기반 기술인 프라이버시를 보장할 수 있는 익명화 기술을 설명한다.

• 전자공학회논문지S
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• 제35S권5호
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• pp.48-58
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• 1998

In this paper, we proposed a new algorithm using characteristics of th ereconstructed phase trajectory by topological mapping developed for a real-tiem detection of the QRS complexes of ECG signals. Using fill-factor algorithm and mutual information algorithm which are in genral used to find out the chaotic characteristics of sampled signals, we inferred the proper mapping parameter, time delay, in ECG signals and investigated QRS detection rates with varying time delay in QRS complex detection. And we compared experimental time dealy with the theoretical one. As a result, it shows that the experimental time dealy which is proper in topological mapping from ECG signals is 20ms and theoretical time delays of fill-factor algorithm and mutual information algorithm are 20.+-.0.76ms and 28.+-.3.51ms, respectively. From these results, we could easily infer that the fill-factor algorithm in topological mapping from one-dimensional sampled ECG signals to two-dimensional vectors, is a useful algorithm for the detemination of the proper ECG signals to two-dimensional vectors, is a useful algorithm for the detemination of the proper time delay. Also with the proposed algorithm which is very simple and robust to low-frequency noise as like baseline wandering, we could detect QRS complex in real-time by simplifying preprocessing stages. For the evaluation, we implemented the proposed algorithm in C-language and applied the MIT/BIH arrhythmia database of 48 patients. The proposed algorithm provides a good performance, a 99.58% detection rate.

• 한국정보전자통신기술학회논문지
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• 제10권1호
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• pp.38-45
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• 2017

정보 처리 기술의 컴퓨터 및 네트워크 의존도가 심화됨에 따라 컴퓨터 및 네트워크에 대한 침입 사례가 갈수록 증가하고 있다. 시스템 및 네트워크의 침입을 방지하기 위하여 호스트와 네트워크 기반 침입차단시스템(방화벽 등)이 개발되었지만 기존의 규칙 기반의 침입차단시스템만으로는 보안 관리에 많은 어려움이 있다. 이러한 이유로 인해 시스템 및 네트워크 자원에 대한 침입을 실시간으로 탐지하고 이에 대처하는 침입탐지시스템 개발에 대한 요구가 증가하고 있다. 본 논문에서는 비선형 자료에도 적용 가능하며 수렴성이 보장된 실시간 특징 추출 방법으로 APEX 알고리즘과 점증적 LS-SVM 분류기를 결합한 실시간 침입탐지 시스템을 개발하였다. 일반적으로 실시간 처리 방식은 메모리의 효율성이 좋고 학습 자료의 추가를 허용하는 장점이 있지만 일괄처리 방식에 비해 정확도가 떨어지는 단점이 있다. 따라서 제안한 시스템은 정확도 면에서도 일괄 처리 방식과 비슷한 성능을 나타내고 있어 상용화가 가능한 시스템이다.

• 정보통신설비학회논문지
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• 제11권1호
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• pp.23-28
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• 2012

In this paper, we proposed and developed a fast and efficient real time face detection and recognition which can be run on embedded system instead of high performance desktop. In the face detection process, we detect a face by finding eye part which is one of the most salient facial features after applying various image processing methods, then in the face recognition, we finally recognize the face by comparing the current face with the prepared face database using a template matching algorithm. Also we optimized the algorithm in our system to be successfully used in the embedded system, and performed the face detection and recognition experiments on the embedded board to verify the performance. The developed method can be applied to automatic door, mobile computing environment and various robot.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.974-977
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• 2003

The GPS/INS integration system navigation can provide improved navigation performance and has been widely used as a main navigation system for military and commercial vehicles. When two navigation systems are tightly coupled and the structure is complicated, a fault in either the GPS or the INS can lead to a disastrous failure of the whole integration system. This paper proposes a real-time fault detection method for an AGPS/INS integration system. The proposed fault detection method comprises a BIT and a fault detection algorithm based on chi-square test. It is implemented by real-time software modules to apply the AGPS/INS integration system and van test is carried out to evaluate its performance.

• 대한임베디드공학회논문지
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• 제3권3호
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• pp.143-150
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• 2008

In this paper, we investigate a real-time environment recognition system based on stereo vision for moving object. This system consists of stereo matching, obstacle detection and distance estimation. In stereo matching part, depth maps can be obtained real road images captured adjustable baseline stereo vision system using belief propagation(BP) algorithm. In detection part, various obstacles are detected using only depth map in case of both v-disparity and column detection method under the real road environment. Finally in estimation part, asymmetric parabola fitting with NCC method improves estimation of obstacle detection. This stereo vision system can be applied to many applications such as unmanned vehicle and robot.

• Journal of Microbiology
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• 제41권4호
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• pp.320-326
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• 2003

In order to identify Vibrio vulnificus in the Yellow Sea near Gunsan, Korea during the early and late summers, the efficiency of the real-time quantitative TaqMan PCR was compared to the efficiency of the conventional PCR and Biolog identification system^TM. Primers and a probe were designed from the hemolysin/cytolysin gene sequence of V. vulnificus strains. The number of positive detections by real-time quantitative TaqMan PCR, conventional PCR, and the Biolog identification system from seawater were 53 (36.8%), 36 (25%), and 10 strains (6.9%), respectively, among 144 samples collected from Yellow Sea near Gunsan, Korea. Thus, the detection method of the real-time quantitative TaqMan PCR assay was more effective in terms of accuracy than that of the conventional PCR and Biolog system. Therefore, our results showed that the real-time TaqMan probe and the primer set developed in this study can be applied successfully as a rapid screening tool for the detection of V. vulnificus.

• 한국항행학회논문지
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• 제12권3호
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• pp.280-285
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• 2008

기존의 얼굴 검출 방법은 프레임 간의 차를 이용하여 움직임을 검출하는 방법이 사용되어 왔다. 그러나, 대부분이 실시간을 고려하지 않은 수학적 접근법을 사용하거나 알고리즘이 지나치게 복잡하여 실시간 구현에 용이하지 않았다. 본 논문에서는 실시간 얼굴검출을 위하여 감시카메라에서 입력된 RGB영상을 YCbCr 영상으로 변환한 후 연속된 두 영상의 차를 구하고 Glassfire 라벨링을 실시했다. 라벨링 결과 가장 넓은 구역의 면적과 Area 임계치 값을 비교하여 임계값 이상의 면적이면 동작변환으로 인식하고 영상을 추출하였다. 이렇게 추출된 동작변환 영상을 대상으로 얼굴 검출을 실시하였다. 얼굴 검출에 필요한 특징을 추출하기 위해 아다부스트 알고리즘을 사용하였다.