• Microbiology and Biotechnology Letters
• /
• v.36 no.1
• /
• pp.12-20
• /
• 2008

Validation of viral safety is essential in ensuring the safety of mammalian cell culture-derived biopharmaceuticals, because numerous adventitious viruses have been contaminated during the manufacture of the products. Mammalian cells are highly susceptible to minute virus of mice(MVM), and there are several reports of MVM contamination during the manufacture of biopharmaceuticals. In order to establish the validation system for the MVM safety, a real-time PCR method was developed for quantitative detection of MVM in cell lines, raw materials, manufacturing processes, and final products as well as MVM clearance validation. Specific primers for amplification of MVM DNA was selected, and MVM DNA was quantified by use of SYBR Green I. The sensitivity of the assay was calculated to be $6{\times}10^{-2}TCID_{50}/mL$. The real-time PCR method was proven to be reproducible and very specific to MVM. The established real-time PCR assay was successfully applied to the validation of Chinese hamster ovary (CHO) cell artificially infected with MVM. MVM DNA could be Quantified in CHO cell as well as culture supernatant. When the real-time PCR assay was applied to the validation of virus removal during a virus filtration process, the result was similar to that of virus infectivity assay. Therefore, it was concluded that this rapid, specific, sensitive, and robust assay could replace infectivity assay for detection and clearance validation of MVM.

• Journal of Life Science
• /
• v.20 no.8
• /
• pp.1201-1206
• /
• 2010

We performed a comparative analysis using a Real-time PCR (Polymerase Chain Reaction) and LC/MS (Liquid-Chromatograph/Mass Spectrometer) method in order to detect microcystin in environmental sources. Among the three different primer sets tested for microcystin using three positive strains of Microcystis aeruginosa by Real-time PCR assay, only TOX2P/TOX2M primer pairs were able to detect Microcystis aeruginosa. According to the results of a survey carried out from June 2009 to September 2009, 11 out of 11 (100%) raw water samples were were found to have microcystin when the Real-Time PCR and LC/MS method was used, with total microcystin concentration ranging from 5.98~219.0 ${\mu}g/l$. A microcystin removal treatment process was used to ensure entire removal, by passing it through a BAC filtration step. It was concluded that real-time PCR assay can be used to estimate micrucystin detection more rapidly and easily than the LC/MS method.

• The KIPS Transactions:PartD
• /
• v.8D no.5
• /
• pp.533-542
• /
• 2001

In this paper, we present a scheduling method for real-time mobile transaction manager in mobile computing environment. The proposed method checks whether a transaction is executable or not. It is able to by considering not only the deadline of real-time data in mobile hosts. And then, it schedules the real-time mobile transactions by making optimal execution window based on the priority queue, while considering transaction value and deadline. Disconnection with mobile hosts is monitored in selecting the transaction for execution. Using the proposed method reduces the number of restarting times after transaction aborts which is caused by the violation of the validity constraints of real-time data. And in has merits of maximizing the sum of values of real-time mobile transactions which meet the deadline. The performance evaluation demonstrates that the number of committed real-time transactions within the deadline is improved by 20%. This method can be used in real-time mobile transaction manager is such environments as cellular communications, emergency medicine information system and so on.

• Journal of IKEEE
• /
• v.24 no.2
• /
• pp.426-434
• /
• 2020

FMI is being researched as a standard for linking large-scale simulation of CPS. In order to guarantee the reliability of the results in large-scale simulations using FMI, event handling through time management techniques is required. This paper aims to guarantee real-time performance and accuracy in large-scale co-simulation environments such as CPS. Synchronize the wallclock time and simulation time to ensure real time. Also, to ensure the accuracy, before the simulation, the event is checked and the simulation is performed with the smallest step size while maintaining the real time until the event occurrence time. As a result, the events occurring in the co-simulation environment are processed immediately and sequentially, ensuring the real-time performance and minimizing the numerical integration error by maximizing the simulation resolution. In the experiment, the proposed method was processed immediately, and it was confirmed that the numerical integration error is reduced by about 1/5 unlike the existing time management method which does not guarantee the resolution.

• Journal of Digital Convergence
• /
• v.10 no.6
• /
• pp.241-246
• /
• 2012

In this paper, we apply the next generation Internet Broadcasting Move Picture solution, FlashVideo has been designed and implemented. Currently being broadcast in the field to compress HD video in real time, as well as live Internet VOD services are available through the online system, the Internet LIVE broadcast and VOD service easy to operate and UCC services that support the solution. VOD video cameras and in real time using H264 CORECODEC to compress MPEC4, WMV, and real-time video streaming on the Internet, and phone system that supports the first, real-time recording of camera images featured nation's first real-time encoder system (Real time encoder system) is, Web and smart environment suitable for supporting the latest CORECODEC technology and software products. Second, the video can be played in MP4 player and customize your chat, and customizing is a possible two-way Internet Broadcasting System. Third, CMS (Contents Management System) feature video contents and course management contents in real time via the Android phone and iPhone streaming service is available.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.9 no.1
• /
• pp.210-216
• /
• 2005

In many real-time applications, security is another important requirement, since the secure real time database system maintains sensitive information to be shared by multiple users with different levels of security clearance or to be attacked by hackers with ill will. Encryption policies are necessary for the security of secure real-time database systems in addition to the existing security methods, too. However, there has not been much work for the encryption policies in secure real-time database systems, although sensitive information must be safeguarded in real-time systems as well. In this paper, we propose a encryption manager for the purpose of solving the encryption policies of the secure real-time database systems. What is important in the encryption policies of secure real-time database systems is security and timeliness. A significant feature of the proposed encryption manager is the ability to dynamically adapt a encryption algorithm that consider transaction deadline and security level.

• KIISE Transactions on Computing Practices
• /
• v.23 no.7
• /
• pp.452-457
• /
• 2017

Linux is a general-purpose operating system that supports several schedulers, allowing different schedulers to coexist. In addition, Linux uses the Control Group (cgroup) to reserve CPU resources for task groups that follow the real-time (SCHED_FIFO, SCHED_RR) and non-real-time (SCHED_NORMAL) scheduler policies, except for the deadline scheduler (SCHED_DEADLINE). The cgroup performs the schedulability analysis to guarantee the reserved CPU resource as much as possible. However, current implementation of the schedulability analysis does not distinguish between deadline tasks and real-time tasks. Therefore, if these deadline tasks and real-time task groups coexist, there is a case where the resource reservation for the real-time task group is rejected. In this paper, we analyze the problems in the schedulability analysis for real-time task groups of Linux cgroups and propose patches to solve these problems.

• Journal of Internet Computing and Services
• /
• v.7 no.3
• /
• pp.107-118
• /
• 2006

Recently, network infra grows rapidly and the digital image compression technique have made remarkable progress, and therefore the demand of the real-time image surveillance system which uses a network camera server is increasing. Network Camera Server has emerged as an attractive alternative to the CCTV for the real-time image surveillance. From this reason, the demand regarding the development of the real-time image surveillance system which uses a network camera server is increasing as well. In this paper, we will provide a model for JPEG Streamer which is used in real-time image surveillance system. And we will design and implement this model. For the stability and efficiency of the JPEG Streamer, we'll use the thread pool and shared memory. We will also introduce the concept of double buffering for increasing the quality of real-time image, Using the JPEG Streamer, a raising of the productivity, reliability and stability will be able to secure to development of real-time image surveillance system.

• The Journal of the Korea Contents Association
• /
• v.20 no.10
• /
• pp.14-24
• /
• 2020

Real-time performance is to return the exact result value to the correct cycle, or to perform the specified work at a certain cycle. Windows does not support real-time performance, so it supports real-time performance using expensive third parties such as RTX and INtime. This paper aims to support real-time performance of Windows through RTiK, a real-time kernel that operates in the form of a device driver in Windows. In Windows 7, RTiK used a timer using local APIC supported by x86 hardware. However, due to the Kernel Patch Protection (KPP) on Windows 10, it became impossible to use a local APIC timer. Therefore, a timer is implemented to inform the determined cycle using Local APIC IPI, and performance measurement is performed to confirm that the cycle operates normally within the error range. This enables real-time performance on Windows 10.

• The Journal of the Korea Contents Association
• /
• v.16 no.5
• /
• pp.217-228
• /
• 2016

Linux it self does not support real-time. To solve this problem RTiK-Linux was designed to support real-time in the kernel space. However, since the user space does not support real-time, it is not easy to develop application. In this paper, we designed and implemented a RTiK-middleware to support real-time in the user space. RTiK-middleware provides real-time scheduling for user space through signal request period after to register process information with request period using apis on application. To evaluate the performance of the proposed RTiK-middleware, we measured the periods of generated real-time threads using RDTSC instructions, and verified that RTiK-middleware operates correctly within the error ranges of 1ms.