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http://dx.doi.org/10.5352/JLS.2010.20.8.1201

Comparative Analysis of Microcystin during Water Treatment Process between Real-Time PCR and LC/MS  

Park, Hong-Gi (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
Jung, Mi-Eun (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
Cha, Dong-Jin (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
Jung, Eun-Young (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
Bean, Jae-Hoon (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
Publication Information
Journal of Life Science / v.20, no.8, 2010 , pp. 1201-1206 More about this Journal
Abstract
We performed a comparative analysis using a Real-time PCR (Polymerase Chain Reaction) and LC/MS (Liquid-Chromatograph/Mass Spectrometer) method in order to detect microcystin in environmental sources. Among the three different primer sets tested for microcystin using three positive strains of Microcystis aeruginosa by Real-time PCR assay, only TOX2P/TOX2M primer pairs were able to detect Microcystis aeruginosa. According to the results of a survey carried out from June 2009 to September 2009, 11 out of 11 (100%) raw water samples were were found to have microcystin when the Real-Time PCR and LC/MS method was used, with total microcystin concentration ranging from 5.98~219.0 ${\mu}g/l$. A microcystin removal treatment process was used to ensure entire removal, by passing it through a BAC filtration step. It was concluded that real-time PCR assay can be used to estimate micrucystin detection more rapidly and easily than the LC/MS method.
Keywords
Microcystin; Real-Time PCR; LC/MS; TOX2P/TOX2M primer;
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