• Journal of English Language & Literature
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• v.64 no.4
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• pp.565-584
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• 2018

The pedagogical dynamic dramatized in Shaw's Pygmalion, which sets man as a distinct pedagogical authority and woman his subject spawning similarly patterned plays many decades later, has been relatively overlooked in the play's criticism clouded by its predominantly mythical theme. Shaw stages Eliza's pedagogical subordination to Higgins followed by her Nora-esque exit with the declaration, "I'll go and be a teacher." The central premise of this article is that the pioneering modern playwright and feminist's pedagogical rewriting of A Doll's House sets out a historical dialogue between Eliza, a new woman who repositions herself as a teacher renouncing her earlier subordinate pedagogical position that is culturally ascribed to women while threatening to replace her paternal teacher, and her immediate precursors, that is, Victorian women teachers whose professional career was socially "anathematized." Through a historical probe into the social status of Victorian women teachers, the article attempts to align their abortive career with Eliza's new womanly re-appropriation of the profession of teaching. With Pygmalion as the starting point of its query, this article conducts a historical survey on the literary representation of pedagogical women from the mid to late Victorian era to the turn of the century. Reading a wide selection of novels and plays alongside of Pygmalion (1912), such as Jane Eyre (1847), A Doll's House (1879), An Enemy of the People (1882), The Odd Women (1893), and The Importance of Being Earnest (1895), it contextualizes Eliza's resolution to be a teacher within the history of female pedagogy. This historical contextualization of the career choice of one of the earliest new women characters in modern drama helps appraise the historical significance of such choice.

• Journal of Korean Library and Information Science Society
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• v.48 no.2
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• pp.121-158
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• 2017

The purpose of this study was to propose the developmental direction for small libraries based on a set of various situational analysis of small libraries in Seoul. By analyzing the operational status of the autonomous regions of different operating methods, we also pursued qualitative enhancement of small libraries by autonomous district by proposing optimized operation methods. As a consequence of the study, it was analyzed that the public and private libraries need to be distinguished in consideration of the characteristics of operation according to the operating entity rather than uniform budget support and guidance management. That is, the small libraries scattered throughout Seoul were founded by the principal of their establishment for the purpose of providing knowledge information and reading culture service within the public activity area. Therefore, if Seoul intervenes and manages under the current situation, where the libraries are operating independently in accordance with the purpose of establishment, regardless of whether they meet the user requirements or not in terms of quality or quantity, various problems may be caused. In this background, it seems appropriate to restrict the library management in line with relevant regulations.

• Journal of the Korean Society of Earth Science Education
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• v.8 no.1
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• pp.66-75
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• 2015

This study is to investigate an argumentation level for elementary school students after analysing an argumentation structure about a socio scientific issue(SSI), and inquire a change of the decision-making according to the refutation materials and empathic situation. This data was collected from the semi-structured interview with the 14 students, grade 6th. For this, the first stage is to present the reading materials about the SSI to the students, let them decision-making and make them argue pro and con about the SSI during the interview. After that, facing with the refutation materials against the existing contention and the empathic situation, the change of the decision-making of the students was conducted. After the research, the level of the argumentation of the students was low, and the students who didn't have the background knowledge about the theme made the decisions according to the values, emotions or intuitions of their own. And the decision-making of the students changed more when presented with the refutation materials and the empathic situation, they were difficult to make the decision because of new information and various stances. Besides, they lacked in the ability that recognize the situation related with their own life, just used the fragmentary knowledge associated with value of life respect. Through these result, it is necessary to produce the refutation materials and empathic materials about the SSI to the students for improving the level of argumentation.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.644-648
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• 2005

Dextranase (${\alpha}$-1,6-D-glucan-6-glucanogydrolase:E.C. 3.2.1.11) catalyzes the hydrolysis of ${\alpha}$-(1.6) linkages of dextran. A lsd1 gene encoding an extracellular dextranase was isolated from the genomic DNA of L. starkeyi. The lsd11 gene is a synthetic dextranase (lsd1) after codon optimization for gene expression with Pichia pastoris system. A open reading frame of lsd11 gene was 1827 bp and it was inserted into the pPIC3.5K expression vector. The plasmid linearized by Sac I was integrated into the 5'AOX region of the chromosomal DNA of P. pastoris. The lsd11 gene fragment encoding a mature protein of 608 amino acids with a predicted molecular weight of 70 kDa, was expressed in the methylotrophic yeast P. pastoris by controling the alcohol oxidase-1 (AOX1) promoter. The recombinant lds11 was optimized by using the shake-flask expression and upscaled using fermentation technology. More than 9.8 mg/L of active dextranase was obtained after induction by methanol. The optimum pH of LSD11 was found to be 5.5 and the optimum temperature $28^{\circ}C$.

• Journal of Environmental Health Sciences
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• v.32 no.5 s.92
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• pp.387-397
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• 2006

This study was performed to investigate the characteristics of indoor air quality(IAQ) in 91 public facilities from September 2004 to March 2005 in the Honam province(Chonnam, Gwangju). The measured air pollutants are $PM_{10}$, formaldehyde(HCHO), CO, $CO_{2}$ and total suspended bacteria(TSB), the maintenance standard materials of IAQ management law established by ministry of environment in Korea(MOE). We also surveyed establishment and operation of ventilation equipment. It was measured $1.2m{\sim}1.5m$ on the floor between 8 o'clock AM and 7 o'clock PM. As the result, $PM_{10}$ and CO was showed the highest concentration, $188.89{\mu}g/m^{3}$, 8.67 ppm, at the indoor parking. The concentration of HCHO was the highest in large store and steamer room. The concentration was respectively $118.70{\mu}g/m^{3}\;and\;113.21{\mu}g/m^{3}$. The concentration of $CO_{2}$ was the highest at the reading room of the library on the condition of natural ventilation. The concentration of $CO_{2}$ was 1,816 ppm and higher than the IAQ standards established by MOE. The TSB was just measured in the hospital and silver town. It was the highest at the admission room of hospital. The concentration of TSB was $766CFU/m^{3}$. The public facilities of this study were not exceed each maintenance standards except $CO_{2}$ but it was surveyed that the management will be needed about some air pollutant according to target facilities.

• Journal of English Language & Literature
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• v.56 no.2
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• pp.187-217
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• 2010

By reading the main character, Rene Gallimard, in M. Butterfly as a spatial metaphor of America, this article examines how homogeneous American national identity of heterosexuality and white masculinity has been reinforced since the cold war and has constituted a crisis of hegemony with the decline of imperialism and how its pathological symptom is shown through the melancholic suicide of Gallimard. This article also argues how the feminine attributes implied in race, gender and sexuality in M. Butterfly are designated and allegorized as an impure, contaminated and ahistorical marker of national integrity in pthe social and material status of the heterosexual American white male. To develop my argument, I read M. Butterfly from a psychoanalytic point of view. Therefore I depend on Freud, Lacan, and Bhabha's psychoanalysis as the theoretical basis. In this paper, I also argue that the homogenized and fixed national identity is splitted and collapsed from within as shown in the Gallimard's melancholy and in the process of splitting the "Third Space" of hybrid subjects for the marginal and the emergent like Song Liling, a homosexual Asian man, can be built "from a space in-between." Therefore Hwang calls into questions conventions of fixed, essentialist identities through the shifting gender identities between Song and Gallimard in M. Butterfly and how identities in the plural are constructed variously in throughly historicized, politicized situations, and these constructions can be complicated by relations of power.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.612-619
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• 2000

To investigate the biochemical properties of V. mimicus metalloprotease, whose gene was isolated previously from Vibrio mimicus ATCC33653, overexpression and purification were attempted. The 1.9 kb of open reading frame was amplified by PCR from pVMC193 plasmid which ligated the VMC gene with pUC19 and introduced into Escherichia coli BL21 (DE3) using the overexpression vector, pET22b (+). The overexpressed metalloprotease (VMC) was purified with Ni-NTA column chromatography and characterized with various protease inhibitors, pHs, temperatures, and substrates. The purified VMC showed the proteolytic activity against gelatin, soluble and insoluble collagens, and synthetic peptides. Unlike the observations made with all metalloproteases originated from other Vibrio sp., the VMC did not hydrolyze the casein. The proteolytic activity was critically decreased when the VMC was treated with metal chelating reagents, such as EDTA, 2,2-bipyridine, and 1, 10-phenanthroline. In particular, the 71 kDa VMC exhibited the hemagglutinating activity against human erythrocyte. As the purified VMC was treated with $CuCl_2$ and $NiCl_2$ for the chemical modification of metal binding, the proteolytic activity and hemagglutinating activity were profoundly influenced. The multialignment analysis made on the reported Vibrio metalloproteases showed the difference of amino acid sequence similarity between the two distinctive classes of Vibrio metalloproteases.

• Progress in Medical Physics
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• v.24 no.3
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• pp.140-144
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• 2013

In this research, the glass dosimeter was calibrated to measure the standard absorbed dose of the Cs-137 irradiator and absorbed dose in a biological sample. Absorbed dose in water for Cs-137 gamma ray was determined by the IAEA TRS-277 protocol. The PTW-TM30013 ion chamber and the PTW-TM41023 water phantom were utilized for measuring absorbed dose and the value was compared with the reading from DoseAce GD-302M glass dosimeter from Asahi Techno Glass Corporation for its calibration. The uncertainty of measurement ($1{\sigma}$) of the calibrated glass dosimeter was 2.7% and this result would be applied to improve the accuracy in measurement of absorbed dose in a biological sample.

• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.399-404
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• 2011

Genetic diversity among 28 Cymbidium varieties was evaluated by using a sequence-related amplified polymorphism (SRAP) marker system. The SRAP marker which was based on the open reading frames (ORFs) regions was developed primarily for Brassica species, but has been applied to various crops. A total of 30 SRAP primer combinations were initially screened. Twenty-eight SRAP primer combinations showed high polymorphism among the 28 Cymbidium varieties, which were consisted of breeding varieties and their parents in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected silver staining method. One hundred ninety six polymorphic bands (7 per primer) were generated and ranged from 0.3 to 1.0 kb in size. Polymorphic fragments were scored for calculating simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. The mean genetic similarity coefficient value was 0.588. The results showed that the correlation between $F_1$ varieties and their parents was high. These studied SRAP markers will be useful tools for genotype identification, germplasm conservation, genetic relationships in Cymbidium.

• Journal of KIISE:Computer Systems and Theory
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• v.27 no.7
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• pp.639-649
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• 2000

In the relational database systems, the join operation is one of the most time-consuming query operations. Many parallel join algorithms have been developed to reduce the execution time. Multiple hash join algorithm using allocation tree is one of most efficient ones. However, it may have some delay on the processing each node of allocation tree, which is occurred in tuple-probing phase by the difference between one page reading time of outer relation and the processing time of already read one. In this paper, to solve the performance degrading problem by the delay, we develop a join algorithm using the concept of 'synchronization of page execution time' for multiple hash joins. We reduce the processing time of each nodes in the allocation tree and improve the total system performance. In addition, we analyze the performance by building the analytical cost model and verify the validity of it by various performance comparison with previous method.