• BMB Reports
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• v.34 no.4
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• pp.371-378
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• 2001

The Glycoprotein D (gD) gene of the HSV-1 strain F was cloned, sequenced, recombinated into the HcNPV (Hyphantria cunea nuclear polyhedrosis virus) expression vector and expressed in insect cells. The gD gene was located in the 6.43 kb BamHI fragment of the strainF. The open reading frame (ORF) of the gD gene was 1,185 by and codes 394 amino acid residues. Recombinant baculoviruses, GD-HcNPVs, expressing the gD protein were constructed. Spodoptera frugiperda cells, infected with the recombinant virus, synthesized a matured gX-gD fusion protein with an approximate molecular weight of 54 kDa and secreted the gD proteins into the culture media by an immunoprecipitation assay The fusion gD protein was localized on the membrane of the insect cells, seen by using an immunofluorescence assay The deduced amino acid sequence presents additional characteristics compatible with the structure of a viral glycoprotein: signal peptide, putative glycosylation sites and a long C-terminal transmembrane sequence. These results indicate the utility of the HcNPV-insect cell system for producing and characterizing eukaryotic proteins.

• Journal of Korean Library and Information Science Society
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• v.35 no.1
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• pp.119-133
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• 2004

This study has compared the job performance of librarian director with that of non-librarian director, by making use of the public library evaluation research executed by Ministry of Culture ＆ Tourism. The result was that the performance score of librarian director is much superior to that of non-librarian director. It is found that the more director's librarianship level is high, the more director's performance score is high. Moreover, it is confirmed a clear relationship between director's performance score and his librarianship level. And so it is evaluated that 'the Library and Reading Promotion Act' which regulates only a librarian to be a public library director is absolutely right.

• The Journal of Korean Society of Virology
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• v.30 no.1
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• pp.1-10
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• 2000

The nonstructural protein NSP4, encoded by gene 10 of rotavirus, has been shown to playa role in viral assembly and known to be an enterotoxin, causing diarrhea in mouse pups. NSP4 gene was cloned from CBNU-2 (virulent bovine rotavirus/diarrheic fecal sample) and CBNU-1 (cell-culture adapted bovine rotavirus/isolated from CBNU-2 and 75 times passaged on MA104 cells), respectively, by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced and compared. The sequence data indicated that the NSP4 genes of bovine rotavirus (BRV) were 751 bases in length and encoded one open reading frame of 175 amino acids beginning at base 42 and terminating at base 569. Differences in nucleotide sequence between CBNU-2 and CBNU-1 were observed at 6 positions (base 274, 296, 391, 394, 396 and 579). NSP4 gene of BRV exhibited a high degree of nucleotide (90% and 94%) and amino acid sequence (91% and 97%) homology with those of SA11 and UK but a low degree of nucleotide (77% and 79%) and amino acids sequence (81% and 85%) homology with those of Wa and OSU.

• Journal of the Korean Society for Library and Information Science
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• v.43 no.1
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• pp.271-292
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• 2009

The public library strives to foster information use by the public, cultural activities, reading, and life-long education. Public libraries are firmly rooted in local societies providing services that reflect the needs and characteristics of those local communities, while at the same time are structured in a coherent nation-wide network. This study examines practices of managing local cultural contents and proposes ways in which public libraries, as central information hubs in local communities, can provide diverse knowledge information and cultural programs, and local culture contents.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.1
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• pp.38-42
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• 2002

A chitinase gene (pCHi58) encoding a 58 kDa chitinase was isolated from the Serratia marcescens KCTC 2172 cosmid library. The chitinase gene consisted of a 1686 bp open reading frame that encoded 562 amino acids. Escherichia coil harboring the pChi58 gene secreted a 58 kDa chitinase into the culture supernatant. The 58 kDa chitinase was purified using a chitin affinity column and mono-S column. A nucleotide and N-terminal amino acid sequence analysis showed that the 58 kDa chitinase had a leader peptide consisting of 23 amino acids which was cleaved prior to the 24th alanine. The 58 KDa chitinase exhibited a $98\%$ similarity to that of S. marcescens OMB 1466 in its nuclotide sequence. The chitinolytic patterns of the 58 kDa chitinase released N,N'-diacetyl chitobiose (NAG2) as the major hydrolysis end-product with a trace amount of N-acetylglucosamine. When a 4-methylumbellyferyl-N-acetylglucosamin monomer, dimmer, and tetramer were used as substrates, the 58 kDa chitinase did not digest the 4-Mu-NAG monomer $(analogue\;of\;NAG_2)$, thereby indicating that the 58 kDa chitinase was likely an endochitinase. The optimum reaction temperature and pH of the enzyme were $50^{\circ}C$ and 5.0, respectively.

• Journal of Microbiology and Biotechnology
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• v.11 no.1
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• pp.65-71
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• 2001

A gene from Paenibacillus sp. KCTC 8848P encoding ${\beta}-amylase$ was cloned and expressed in Escherichia coli. The Paenibacillus ${\beta}-amylase$ gene cosisted of a 2,409-bp open reading frame without a translational stop codon, encoding a protein of 803 amino acids. The presumed ribosime-binding site, GGAGG, was located 10 bp upstream from the TTG initiation codon. The deduced amino acid sequence of the ${\beta}-amylase$ gene had a 95% similarity to the ${\beta}-amylase$ of Bacillus firmus. The ${\beta}-amylase$ gene was introduced into wild-type strains of Saccharomyces cerevisiae using a linearized yeast integrating vector containing a geneticin resistance gene and its product was secreted into the culture medium.

• English Language & Literature Teaching
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• v.11 no.2
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• pp.171-194
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• 2005

The essay attempts at a critical reading of Thomas Hardy's The Mayor of Casterbridge (1886) in terms of character and socio-cultural change. Juxtaposing the story of Michael Henchard's career with the social and economic changes in the agricultural town, it attempts to elaborate on the complex ways in which Hardy relates the old modes of life and thinking to the material culture. Though the novel is centered on the story of Henchard, the Henchard-Farfrae clash represents the conflict of "old" and "new" modes of socio-economic organization and consciousness. The story of the rustic man of character struggling with his contradictory traits of strong will-power and emotional collapse suggests that Hardy's literary representation of the rural community and the rustic protagonist is deeply rooted in historical reality. However, while there is the interlocking of the changes in personal fate and social change, the representation is a "reinvented" literary construction with complex mediation. Despite the narrator's emphasis on Henchard's immutability, peculiarity, and resilience, his character is, in a complex, mediated way, shaped by the material conditions of English rural community in the late 19th century. The mediating role of Elizabeth-Jane as a narrative resolution embodies Hardy's ambivalent historical position concerning the period undergoing change and conflict.

• International Journal of Knowledge Content Development & Technology
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• v.8 no.2
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• pp.67-100
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• 2018

This study comprehensively draws a conclusion using factors of economic, social, and educational value and performed a survey on librarians and users to compare and analyze the difference in perception between the two groups. The result are as follows: First, Libraries had the social value not only on research and personal learning but also on establishment and vitalization of communities, provision of opportunities to share ideas between local residents and create something new, and provision of recreation to improve the residents' capability. Second, both groups evaluated the libraries' educational role and function relatively highly. In particular, the librarians scored very high for the items that the libraries' educational role was expanding, the libraries were a part of the educational system, and the libraries' value on reading and literacy was great, and the users thought that libraries had an value because they were a part of education and the educational system. Third, economic value part also obtained relatively lower scores than other values; the mean of the librarian group was 3.46 and the user group was 3.43. Among the economic values, the librarian group scored the highest for the area of economic value for local communities, and the greatest scores were given to manpower development by the users.

• Journal of the Korean Society for Library and Information Science
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• v.50 no.2
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• pp.271-287
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• 2016

This study intends to review the quality factors of library service for the disabled using user-centered approach. For the empirical review of quality factors, Nine quality factors were drawn after analyzing the current library service quality factors and the standards of library service for the disabled. Using these factors, The survey for identifying importance of factors and the qualitative evaluation of service cases were performed in this study. As a result of this survey, the most critical but relatively weak quality factors were facility, collection and staff factors. And the qualitative evaluation results of service cases indicated that the reading culture program and the information service factors were critical.

• Korean Institute of Interior Design Journal
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• v.21 no.3
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• pp.174-181
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• 2012

In 2010 there are 759 public libraries in Korea, and they have been increased constantly for the recent four to five years. In order to make them as cultural spaces in local communities, Korean government is now planning to increase the number of public libraries up to 900 and to build small to medium-sized multiple purpose libraries rather than large and single purpose ones. Especially in the case of public libraries in metropolitan cities, which are main libraries in those areas, they should be designed to play major functions of cultural experience, exhibition, and life-long education as well as to meet the basic needs of providing books for public reading and of building and developing branch libraries based on local characteristics. Above all, in order to make a public library as a center of local culture, a space planning focused on increasing utilization and satisfaction of the residents is required. It is essential to conduct a survey to understand the needs of the residential users and reflect their opinions. In this regard, our research process was constructed as follows. First, precedent studies on a certain densely populated area were reviewed. Second, a survey was conducted in Ulsan city to figure out the needs and the satisfaction level of the actual users. The purpose of this study is to propose a better space planning approach for public libraries in metropolitan cities based on the analysis.