• 한국도서관정보학회지
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• 제37권4호
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• pp.111-141
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• 2006

본 연구는 학교도서관 중심의 독서교육을 위한 내용체계 즉, 범위와 계열을 제안하는데 목적이 있다. 기존의 독서교육 내용체계를 비교 분석하여 공통적인 요소를 추출하고, 이를 바탕으로 우리나라의 실정에 맞는 독서교육의 내용체계 즉, 범위와 계열을 설정하였다. 우리나라의 고등학교 독서 교과의 교육과정은 물론 미국과 일본의 대표적인 독서 관련 교육과정의 내용체계를 비교 분석하였다. 분석 결과를 바탕으로 10개 영역, 45개 요소의 독서교육 내용(요소)을 1차적으로 설정하였다. 초 중등학교 현장의 사서교사를 대상으로 설문조사하여 1차적으로 설정한 독서교육의 범위(요소)에 대한 타당성과 지도하기에 적합한 학교 급별 수준을 조사한 후 최종적으로 학교도서관 중심의 독서교육 내용체계 즉, 범위와 계열을 제안하였다.

• 한국비블리아학회지
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• 제16권1호
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• pp.45-74
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• 2005

본 연구에서는 전통적인 도서관이용교육과 독서교육 그리고 정보활용교육을 종합적으로 운영할 수 있는 전략으로서 교육과정의 내용 체계 즉, 범위와 계열을 설정하였다. 한국과 일본 그리고 미국에서 적용되고 있는 정보활용교육의 사례를 비교${\cdot}$분석하고, 분석 내용을 바탕으로 정보활용교육을 위한 교육과정의 범위와 계열을 설정하였다. 새롭게 설정한 정보활용교육의 범위와 계열은 정보활용교육의 모형에서 추출한 5대 기능과 과정을 근간으로 하였으며, 전통적인 도서관이용교육과 독서교육의 내용을 수용하였다. 또한, 모든 정보매체와 정보텍스트의 형식, 정보활용의 과정과 전략, 정보의 체계 및 구조를 종합적으로 지도할 수 있도록 편성하였으며, 초등학교 저학년(1-3), 고학년(4-6), 중학교(1-3), 고등학교(1-3)로 구분하여 교육내용에 따른 계열을 설정하였다.

• 한국초등수학교육학회지
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• 제17권2호
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• pp.207-223
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• 2013

이 논문은 5+2=7과 같은 등호가 들어 있는 식의 읽기와 쓰기라는 두 행위 사이의 불일치 및 그 해소 과정에서 생길 수 있는 문제점에 관하여 논한 것이다. 기호 이해의 시간적 차원과 등호 개념의 이중성을 바탕으로, 초등 수학 교과서에 제시된 등식 읽기와 쓰기 방법을 분석하였다. 교사는 수업에서 기호 읽기와 기호 쓰기를 통해 무시간적인 차원의 기호를 시간 속에 펼쳐 놓는 시간화 작업을 수행한다. 이 때 읽기 순서와 쓰기 순서 사이에 불일치가 있을 수 있으며, 이를 교사가 어떻게 해소하는가는 학생들의 기호 이해에 영향을 줄 수 있다. 등식 읽기를 쓰기 관습에 종속시켜 이 불일치를 해소하면, 관계적 관점을 나타내고 있는 교과서의 등식 읽기를 조작적 관점의 읽기로 변환하는 현상이 일어나게 된다. 등호의 관계적 의미 이해를 중시하는 입장에서 보면, 쓰기를 교과서에 제시된 읽기 방식에 종속시키는 방향으로 불일치를 해소하는 것이 적절하다. 또한, 등호의 읽기 쓰기를 부등호의 읽기 쓰기와 통합적으로 다룰 필요가 있다.

• 미생물학회지
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• 제42권1호
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• pp.67-72
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• 2006

클로닝된 Bacillus circulans ATCC21367 유래 cellulolytic xylanase 유전자(bglBC2)의 염기서열을 결정 분석하였다. 본 유전자는 1,224 bp의 407개 아미노산을 암호하는 open reading frame (ORF)으로 구성되어 있었으며 염기서열로부터 산출된 유전자의 분자량은 45 kDa으로 효소의 SDS-PAGE로부터 측정된 분자량과 일치하였다. ATG 개시 코돈의 9bp 위쪽에 Shine-Dalgarno (SD) 서열로 추정되는 5'-AAAGGAG-3' 서열이 확인되었고 그 상단에 promoter로 추정되는 -35 서열(TTTACA)과 -10 서열(TATACT)이 위치하고 있었으며, 이는 B. subtilis promoter consensus sequence와 유사하였다. 한편, 이 효소의 아미노산 서열은 이미 보고된 B. circulans KSM-N257의 alkaline $endo-\beta-1,4-glucanase$와는 97%, B. circulans WL-12의 $endo-\beta-1,3-1,4-glucanase$와는 75%, Bacillus sp. KSM-330의 $endo-\beta-1,4-glucanase$ (cellulase)와는 45%의 유사성을 나타내었다. 또한 bglBCS 염기서 열의 정보를 GenBank에 등록하였으며 등록번호는 Ar269256이다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.758-763
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• 2012

As a member of a subclass of immunophilins, it is controversial that FKBP38 acts an upstream regulator of mTOR signaling pathway, which control the process of cell-growth, proliferation and differentiation. In order to explore the relationship between FKBP38 and mTOR in the Cashmere goat (Capra hircus) cells, a full-length cDNA was cloned (GenBank accession number JF714970) and expression pattern was analyzed. The cloned FKBP38 gene is 1,248 bp in length, containing an open reading frame (ORF) from nucleotide 13 to 1,248 which encodes 411 amino acids, and 12 nucleotides in front of the initiation codon. The full cDNA sequence shares 98% identity with cattle, 94% with horse and 90% with human. The putative amino acid sequence shows the higher homology which is 98%, 97% and 94%, correspondingly. The bioinformatics analysis showed that FKBP38 contained a FKBP_C domain, two TPR domains and a TM domain. Psite analysis suggested that the ORF encoding protein contained a leucine-zipper pattern and a Prenyl group binding site (CAAX box). Tissue-specific expression analysis was performed by semi-quantitative RT-PCR and showed that the FKBP38 expression was detected in all the tested tissues and the highest level of mRNA accumulation was detected in testis, suggesting that FKBP38 plays an important role in goat cells.

• Journal of Microbiology
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• 제45권2호
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• pp.153-157
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• 2007

5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EC 2.5.1.19) is the sixth enzyme in the shikimate pathway which is essential for the synthesis of aromatic amino acids and many secondary metabolites. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide glyphosate. In this study, the Dunaliella salina EPSP synthase gene was cloned by RT-PCR approach. It contains an open reading frame encoding a protein of 514 amino acids with a calculated molecular weight of 54.6 KDa. The derived amino acid sequence showed high homology with other EPSP synthases. The Dunaliella salina EPSP synthase gene was expressed in Escherichia coli and the recombinant EPSP synthase were identified by functional complementation assay.

• Animal cells and systems
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• 제10권4호
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• pp.197-201
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• 2006

Diaminopimelate decarboxylase (DAPDC, EC 4.1.1.20) catalyzes the conversion of diaminopimelate into lysine (Lys), which is the last step in Lys biosynthetic pathway. The genes for DAPDC have been reported in many bacteria, and more recently in Arabidopsis. Here we report characterization of a gene for DAPDC from rice (OsDAPDC). Sequence analysis of a cDNA clone revealed a full-length open reading frame for OsDAPDC that encoded 490 amino acids, approximately 53.2 kDa protein. The OsDAPDC protein contains a consensus binding site for pyridoxal-5'-phosphate as a cofactor and has a sequence at the amino terminus that resembles a transit peptide for localization to plastids, similar to that of Arabidopsis. Single gene encoding DAPDC was found in chromosome II in rice. The predicted amino acid sequence of OsDAPDC is highly homologous to that of the enzymes for DAPDC encoded by lysA of many bacteria. Expression of OsDAPDC in lysA mutants of Escherichia coli shows that the gene is able to functionally complement the mutants. These results suggest that OsDAPDC encodes a protein for diaminopimelate decarboxylase in rice.

• International Journal of Industrial Entomology and Biomaterials
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• 제21권1호
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• pp.127-132
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• 2010

We have characterized full-length cDNA encoding Gall-6-tox protein, which was cloned from the fat body of the immunized Galleria mellonella larvae. The cloned cDNA of Gall-6-tox consists of 1301 nucleotides and contained an open reading frame of 891 nucleotides corresponding to a protein of 296 residues that includes a putative 16-residue signal sequence and a 280-residue mature peptide with a calculated mass of 30,707.73 Da. The deduced mature peptide contains conserved tandem repeats of six cysteine-stabilized alpha beta ($Cs{\alpha}{\beta}$) motifs, which was detected in scorpion toxins and insect defensins. In the sequence homology search, mature Gall-6-tox showed 34% and 28% amino acid sequence homology with Bomb-6-tox from Bombyx mori and Spod-11-tox from Spodoptera frugiperda, respectively. Gall-6-tox orthologs were only found in Lepidopteran species, indicating that this new immune-related gene family is specific to this insect order. RT-PCR analysis revealed that Gall-6-tox was expressed primarily in the larval fat bodies, hemocytes, and midgut against invading bacteria into hemocoel. Moreover, the expression time course of Gall-6-tox was examined up to 24 h in the fat bodies and midgut after injection of E. coli. Altogether, these results suggest that Gall-6-tox is derived from defensins and Gall-6-tox may play a critical role in Lepidoptera immune system.

• 환경생물
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• 제27권4호
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• pp.406-413
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• 2009

The enzyme invertase contributes to sugar unloading, pathogen defense, differentiation and development in plants. We cloned the complete cDNA of a soluble acid invertase from pea seedlings (Pisum sativum) via RT-PCR and the rapid amplification of the cDNA end (RACE) technique. The full-length cDNA of the soluble pea invertase comprised 2237 bp and contained a complete open reading frame encoding 647 amino acids. The deduced amino acid sequence showed high homology to soluble acid invertases from various plants. Northern blot analysis demonstrated the soluble acid invertase gene of P. sativum was strongly expressed in sink organs such as shoot tips and root tips, and induced by abscisic acid, gibberellic acid and jasmonic acid in shoots. Especially, gibberellic acid enhanced the gene expression of the soluble acid invertase in a time-dependent manner. This study presents that the gene expression patterns of a soluble acid invertase from pea are strongly consistent with the suggestion that individual invertase gene product has different functions in the growing plant.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.917-922
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• 2008

A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events that control ovulation. In this study, a 9,026-bp DNA sequence containing the full coding region, all 8 introns and part of the 5'and 3' untranslated region of the porcine ADAMTS1 gene was obtained. Analysis of the ADAMTS1 gene using the porcine radiation hybrid panel indicated that pig ADAMTS1 is closely linkage with microsatellite marker S0215, located on SSC13q49. The open reading frame of its cDNA covered 2,844 bp and encoded 947 amino acids. The coding region of porcine ADAMTS1 as determined by sequence alignments shared 85% and 81% identity with human and mouse cDNAs, respectively. The deduced protein contained 947 amino acids showing 85% sequence similarity both to the human and mouse proteins, respectively. Comparative sequencing of three pig breeds revealed one single nucleotide polymorphism (SNP) within exon 7 of which a G-C substitution at position 6006 changes a codon for arginine into a codon for proline. The substitution was situated within a PvuII recognition site and developed as a PCR-RFLP marker for further use in population variation investigations and association analysis with litter size. Allele frequencies of this SNP were investigated in seven pig breeds/lines. An association analysis in a new Qingping female line suggested that different ADAMTS1 genotypes have significant differences in litter size (p<0.01).