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Cloning and Characterization of Novel Soluble Acid Invertase Which is Responsible to JA, ABA and GA During Tip Growth of Pea Seedlings (Pisum sativum)  

Kim, Dong-Giun (Department of Science Education, College of Education, Kyung-Nam University)
Zhang, Jiesheng (Department of Science Education, College of Education, Kyung-Nam University)
Publication Information
Korean Journal of Environmental Biology / v.27, no.4, 2009 , pp. 406-413 More about this Journal
Abstract
The enzyme invertase contributes to sugar unloading, pathogen defense, differentiation and development in plants. We cloned the complete cDNA of a soluble acid invertase from pea seedlings (Pisum sativum) via RT-PCR and the rapid amplification of the cDNA end (RACE) technique. The full-length cDNA of the soluble pea invertase comprised 2237 bp and contained a complete open reading frame encoding 647 amino acids. The deduced amino acid sequence showed high homology to soluble acid invertases from various plants. Northern blot analysis demonstrated the soluble acid invertase gene of P. sativum was strongly expressed in sink organs such as shoot tips and root tips, and induced by abscisic acid, gibberellic acid and jasmonic acid in shoots. Especially, gibberellic acid enhanced the gene expression of the soluble acid invertase in a time-dependent manner. This study presents that the gene expression patterns of a soluble acid invertase from pea are strongly consistent with the suggestion that individual invertase gene product has different functions in the growing plant.
Keywords
gene expression; gibberellic acid; pea; soluble acid invertase;
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1 ap Rees T. 1988. Hexose Phosphate Metabolism by Nonphotosynthetic Tissues of Higher Plants; in The Biochemistry of Plants Carbohydrates, pp.1-84, Academic Press, San Diego
2 Copeland L. 1990. Enzymes of sucrose metabolism; in Methods in Plant Biochemistry, pp.74-85, Academic Press, San Diego
3 Fahrendorf T and E Beck. 1990. Cytosolic and cell wall-bound acid invertase from leaves of Uritica dioico L. Planta 180:237-244
4 Mitsuhashi W, S Sasaki, A Kanazawa, YY Yang, Y Kamiya and T Toyomasu. 2004. Differential expression of acid invertase genes during seed germination in Arabidopsis thaliana. Biosci. Biotech. Biochem. 68:602-608   DOI   ScienceOn
5 Ross H, D McRae and H Davies. 1996. Sucrolytic enzyme activity in cytoledons of the faba bean. Plant Physiol. 111:329-338
6 Sturm A and M Chrispeels. 1990. cDNA cloning of carrot extracellular-fructofuranosidase and its expression in response to wounding and infection. Plant Cell 2:1107-1119
7 Tymowska-Lalanne Z and M Kreis. 1998a. Expression of the Arabidoposis thaliana invertase gene family. Planta 207:259-265   DOI   ScienceOn
8 Wu LL, JP Mitchell, NS Cohn and PB Kaufman. 1993. Gibberellin (GA3) enhances cell-wall invertase activity and mRNA level in elongating dwarf pea (Pisum sativum) shoots. Int. J. Plant Sci. 154:280-289   DOI   ScienceOn
9 Frommer WB and U Sonnewald. 1995. Molecular analysis of carbon partitioning in solanaceous species. J. Exp. Bot. 46:587-607   DOI   ScienceOn
10 Koch K. 2004. Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Curr. Opin. Plant Biol. 7:235-246   DOI   ScienceOn
11 Weber H, L Borisjuk, U Heim, P Buchner and U. Wobus. 1995. Seed coat-associated invertases of fava bean control both unloading and storage functions: cloning of cDNAs and cell type-specific expression. Plant Cell Physiol. 7:1835-1846
12 Roitsch T, M Bittner and D Godt. 1995. Induction of apoplastic invertase of Chenopodium rubrum by D-glucose and a glucose analog and tissue-specific expression suggest a role in sink-source regulation. Plant Physiol. 108:285-294   DOI   ScienceOn
13 Estruch JJ and JP Beltran. 1991. Changes in invertase activities precede ovary growth induced by gibberellic acid in Pisum sativum. Physiol. Plant 81:319-326   DOI   ScienceOn
14 Cuellar-Ortiz SM, M De La Paz Arrieta-Montiel, J Acosta-Gallegos and AA Covarrubias. 2008. Relationship between carbohydrate partitioning and drought resistance in common bean. Plant, Cell Environ. 31:1399-409   DOI   ScienceOn
15 Fernie AR, L Willmitzer and RN Trethewey. 2002. Sucrose to starch: a transition in molecular plant physiology. Trends Plant Sci. 7:35-41   DOI   ScienceOn
16 Godt D and T Roitsch. 1997. Regulation and tissue-specific distribution of mRNAs for three extracellular invertase isoenzymes of tomato suggests an important function in establishing and maintaining sink metabolism. Plant Physiol. 115:273-282   DOI   ScienceOn
17 Roitsch T and MC Gonzalez. 2004. Function and regulation of plant invertases: sweet sensations. Trends Plant Sci. 9:606-613   DOI   ScienceOn
18 Linden JC, R Ehneb and T Roitsch. 1996. Ethylene regulation of apoplastic invertase expression in autotropic cells of Chenopodium rubrum. Plant Growth Reg. 19:219-222   DOI   ScienceOn
19 Moll VA. 1971. Soluble invertase in dwarf pea internodes during normal and gibberellin stimulated growth. Biochim. Physiol. Pflanzen. 162:334-342
20 Zhang L, NS Cohn and JP Mitchell. 1997. A pea cell-wall invertase gene (PSInv-1) with tissue-specific expression. Plant Physiol. Biochem. 35:751-760
21 Zhang L, NS Cohn and JP Mitchell. 1996. Induction of a pea cell-wall invertase gene by wounding and its localization and expression in phloem. Plant Physiol. 112:1111-1117
22 Sonnewald U, M Hajirezaei, J Kossmann, A Heyer, RN Trethewey and L Willmitzer. 1997. Increased potato tuber size resulting from apoplastic expression of a yeast invertase. Nat. Biotechnol. 15:794-797   DOI   ScienceOn
23 Xu J, WT Avigne, DR McCary and KE Koch. 1996. A similar dichotomy of sugar modulation and developmental expression affects both paths of sucrose metabolism: evidence from a maize invertase gene family. Plant Cell Physiol. 8:1209-1220
24 Tymowska-Lalanne Z and M Kreis. 1998b. The plant invertases: physiology, biochemistry and molecular biology. Advan. in Botan. Res. 28:72-117
25 Andersen MN, F Asch, Y Wu, CR Jensen, H Nested, VO Mogensen, and KE Koch. 2002. Soluble Invertase Expression Is an Early Target of Drought Stress during the Critical, Abortion-Sensitive Phase of Young Ovary Development in Maize1. Plant Physiol. 130:591-604   DOI   ScienceOn
26 Sturm A, V Sebkova, K Lorenz, M Hardegger, S Lienhard, and C Unger. 1995. Development- and organ-specific expression of the genes for sucrose synthase and three isoenzymes of acid-fructofuranosidase in carrot. Planta 195:601-610   DOI   ScienceOn
27 Proels RK, B Hause, S Berger and T Roitsch. 2003. Novel mode of hormone induction of tandem tomato invertase genes in floral tissues. Plant Mol. Biol. 52:191-201   DOI   ScienceOn
28 Unger C, M Hardegger, S Lienhard and A Sturm. 1994. cDNA cloning of carrot (Daucus carota) soluble acid-fructofuranosidase and comparison with the cell wall isoenzyme. Plant. Physiol. 104:1351-1357   DOI   ScienceOn