• Journal of Korean Library and Information Science Society
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• v.48 no.2
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• pp.121-158
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• 2017

The purpose of this study was to propose the developmental direction for small libraries based on a set of various situational analysis of small libraries in Seoul. By analyzing the operational status of the autonomous regions of different operating methods, we also pursued qualitative enhancement of small libraries by autonomous district by proposing optimized operation methods. As a consequence of the study, it was analyzed that the public and private libraries need to be distinguished in consideration of the characteristics of operation according to the operating entity rather than uniform budget support and guidance management. That is, the small libraries scattered throughout Seoul were founded by the principal of their establishment for the purpose of providing knowledge information and reading culture service within the public activity area. Therefore, if Seoul intervenes and manages under the current situation, where the libraries are operating independently in accordance with the purpose of establishment, regardless of whether they meet the user requirements or not in terms of quality or quantity, various problems may be caused. In this background, it seems appropriate to restrict the library management in line with relevant regulations.

• Journal of Haehwa Medicine
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• v.20 no.1
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• pp.25-37
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• 2011

It fuses into culture of the East and the West in use of color ; colors of five direction, which had been from the Five Phase Theory in East Asia. Formative ability of color fields in a formative early Oriental Medicine history as well. Color is a very important factor and a necessary step of visible diagnosis. As a human's body is maintained and alive under the control of spirit and spirit is stored by five viscera, so spirit expresses the change of five viscera and is reflected by color. Visible diagnosis consists of spirit, appearance, Qi and color and movement. The purpose of each visible diagnosis is that we would know states of essence, Qi and spirit in patient's body. To ancient Asia people 'to see' was a kind of insight to the object as a whole. Similarily the activity of seeing the human body was the integral part of making diagnosis of a patient. This was the cause that there was suggested the discussion of the Five color theory in Oriental Medicine as a counterpart to that of the 'Goethe for Zur Farbenlehrer'. The inspection of Oriental Medicine was not a simple gazing of the eye as a sense organ, but the total insight to the internal state of the patient. For that reason, the eye－perception in early Chinese medicine was the reading the signs of the internal body which had have not the visual form but the flux of the internal life.

• Korean Journal of Health Education and Promotion
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• v.11 no.2
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• pp.68-86
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• 1994

In order to provide data of effective health education for practice of preventive health behavior for visual acuity care. The authors investgated the actual acuity and health behavior for visual acuity care. The subjects of this study were 2250 students in high school. The data were collected by questionnaire developed for the purpose of this study which has been made through references and student's health record. The results were as follows, 1) As students move up to grade, the number of students with decreased visual acuity had a tendency to increase. In cases with decreased visual acuity below 0.2 in one or both eye, the number of cases was increased in accordance with moving up to grade, especially visual acuity of right eye was more decreased than that of left eye. 2) As to proportion of the group with decreased visual acuity among the total students, among 2250 students, students with decreased visual acuity was 35.6%(800 students). According to school type, in academic school, frequency of students with decreased visual acuity in day school was higher then that in night school. In commercial school, the frequency of students who had decreased visual acuity in night school were higher than that of students in day school. 3) As to variables influencing the decreased visual activity, there was a significant different between groups with normal visual acuity and groups with subnormal in terms of awareness of visual acuity by themself, parent's visual acuity, unbalanced eating habit, distance from television, or books, posture in watching television, posture in reading, awareness of illumination in classroom. 4) As to practice of protective and preventive health behavior for visual acuity care, In group with normal visual acuity. There was good practice such as proper eating habit, proper studying habit, and health habit in its descending order, but proper illumination and regular eye test were practiced poorly. In group with subnormal visual acuity, one of the vest practice in visual acuity care was an effort to prevent eye strain. Other good practice involve tasking regular eye test, maintaining proper studing habit, in its descending order.

• Research in Community and Public Health Nursing
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• v.12 no.2
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• pp.491-503
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• 2001

Purpose: The purpose of this study is to identify the health promoting lifestyle of Korean immigrants and to develop the health promotion program for Korean immigrants. Method: The subject of the study were 207 adults chosen from Korean religious organizations located in Chicago area. The instrument used in this study was Health Promoting Lifestyle Profile (HPLP) by Walker. Sechrist & Pender(l995). The data were collected between August 1 and October 20. 2000 by using self-administered questionnaire. Analysis of data was done by using descriptive statistics. Pearson correlation coefficient, t-test, ANOVA. Duncan test and stepwise multiple regression with SPSS program. Result. 1) The average score of performance in the health promoting lifestyle 2.43 scores. In the subscales, the highest degree of performance was 'spiritual growth', following 'nutrition', 'interpersonal relationship', 'stress management' and 'health responsibility' and the lowest degree of performance was 'physical activity'. 2) Health promoting lifestyle was significantly correlated with such demographic variables as age (F=2.659. p=.049), family income(F=4.696. p = .027), subjective health status(F = 3.882. p=.005), the frequency of pray(F=9.442. p = .000), the frequency of reading the bible(F=8.584. p= .000) and years of residence in the US(F=4.273. p= .015). 3) Health promoting lifestyle was significantly predicted by the frequency of pray, subjective health status. current working status, taking medication, level of education and family income. These variables explained 27.4% of variance of health promoting lifestyle. Conclusion The above findings indicate that it is necessary to develop a health promotion program facilitating exercise and enhancing health responsibility for Korean immigrants. It is suggested that the comparative study to identify the differences and similarities between Korean immigrants in the U.S.A. and Korean residents in Korea.

• Journal of Korean Home Economics Education Association
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• v.23 no.2
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• pp.131-148
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• 2011

The purpose of this study was to analyze the contents of housing area in Technology Home Economics textbooks of the 2007 revised curriculum which was published in 2011. The structure and the contents of housing area which are consisted of housing/living environment and use of housing space were analyzed. This study aims to examine the changes in the newly revised textbooks as well as suggesting advice for the more improved revised one in the future. For this purpose, this study employed 11 different textbooks and the results were the followings: each textbook had different quantity of pages dedicated to housing area but commonly had the wide range of terminology, plentiful amount of tables/diagrams, reading and activity materials sufficient to have students to carry out in practice. Also, the newly added contents of local area and eco-friendly life are analyzed to be well reflecting the 2007 revised curriculum. In future textbook development, it will be important that the issue of low birth rate, population ageing and green-life to be included as well as emphasizing the content of the network within the local area in order to actively prepare for the environmental issues.

• Journal of Life Science
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• v.8 no.5
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• pp.604-613
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• 1998

The last enzyme of the sesquiterpen phytoalexin capsidiol synthesis in tobacco cell, 5-epi-aristolochene hydro-xylase which convert 5-epi-aristolochene (EAS) to capsidiol, was cloned by a reverse transcription polymerase chain reaction strategy and cDNA library screening. Cloned CYP-B3 contained high probability amino acid matches to known plant cytochrome P450 sequences and open reading frame with the conserved FxxGxRxCxG heme-binding region. Transcripts of CYP-B3 were not detected in control cells, but induced in elicitor-treated cells. Furthermore, CYP-B3 transcripts were induced by fungal extracts and cellulase but not by other stimuli(chilling, heat shock and 2,4-D). Induction of CYP-B3 transcripts by elicitor treatment was not affected by ancymidol and ketoconazole treat-ments suggesting that an inhibition of hydroxylase activity by Cyt P450 inhibitors resulting from post translational processing event.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.12
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• pp.1774-1783
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• 2015

Milk lysozyme is the ubiquitous enzyme in milk of mammals. In this study, the cDNA sequence of a new chicken-type (c-type) milk lysozyme gene (YML), was cloned from yak mammary gland tissue. A 444 bp open reading frames, which encodes 148 amino acids (16.54 kDa) with a signal peptide of 18 amino acids, was sequenced. Further analysis indicated that the nucleic acid and amino acid sequences identities between yak and cow milk lysozyme were 89.04% and 80.41%, respectively. Recombinant yak milk lysozyme (rYML) was produced by Escherichia coli BL21 and Pichia pastoris X33. The highest lysozyme activity was detected for heterologous protein rYML5 (M = 1,864.24 U/mg, SD = 25.75) which was expressed in P. pastoris with expression vector $pPICZ{\alpha}A$ and it clearly inhibited growth of Staphylococcus aureus. Result of the YML gene expression using quantitative polymerase chain reaction showed that the YML gene was up-regulated to maximum at 30 day postpartum, that is, comparatively high YML can be found in initial milk production. The phylogenetic tree indicated that the amino acid sequence was similar to cow kidney lysozyme, which implied that the YML may have diverged from a different ancestor gene such as cow mammary glands. In our study, we suggest that YML be a new c-type lysozyme expressed in yak mammary glands that plays a role as host immunity.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.243-249
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• 2012

Hexokinase is the first enzyme in the hexose assimilation pathway; it acts as a sensor for plant sugar responses, and it is also important in determining the fruit sugar levels. The full-length cDNA of a hexokinase gene was isolated from loquat through reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends, which was designated as EjHXK1. EjHXK1 is 1,839 bp long and contains an entire open reading frame encoding 497 amino acids. The predicted protein of EjHXK1 shares 72%-81% similarity with other plant hexokinases. Phylogeny analysis indicated that EjHXK1 is closely related to maize and rice hexokinases. Transient expression of the 35S: EjHXK1-GFP fusion protein was observed on the cell membrane and cytoplasm. Real-time RT-PCR indicated that EjHXK1 is expressed in loquat leaves, stems, flowers, and fruits. EjHXK1 transcripts were higher during early fruit development, but decreases before maturation, which is consistent with hexokinase enzyme activity during fruit development and conducive for hexose accumulation in mature fruits. These results imply that EjHXK1 may play important roles in the regulation of sugar flux during fruit ripening.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.2
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• pp.127-131
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• 2003

Protein disulfide isomerase (PDI) found in the endoplasmic reticulum (ER) catalyzes disulfide bond exchange and assists in protein folding of newly synthesized proteins. PDI also functions as a molecular chaperone and has been found to be associated with proteins in the ER. In addition, PDI functions as a subunit of two more complex enzyme systems: the prolyl-4-hydroxylase and the triacylglycerol transfer proteins. A cDNA that encodes protein disulfide isomerase was previously isolated from Bombyx mori (bPDI), in which open reading frame of 494 amino acids contained two PDI-typical thioredoxin active site of WCGHCK and an ER retention signal of the KDEL motif at its C-terminal, and we report its functional characterization here. This putative bPDI cDNA is expressed in insect Sf9 cells as a recombinant proteins using baculovirus expression vector system. The bPDI recombinant proteins are successfully recognized by antirat PDI antibody, and shown to be biologically active in vitro by mediating the oxidative refolding of reduced and scrambled RNase. This suggests that bPDI may play an important role in protein folding mechanism of insects.

• Journal of the Korean housing association
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• v.24 no.1
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• pp.1-10
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• 2013

Number of children cared by child care centers has getting up almost half of the from zero to five year age group in korea. Home care children' activities are reported more active and natural than those of center care children. So this study seek the design guidelines for the residential core model of child care centers as second home in korea. The residential core model by Anita Lui Olds was selected and ten domestic center cases were surveyed for guidelines. Firstly, daily-residential core model is learning by daily life at home, and is equiped with cooking kitchenet and group activity area in group room. Secondly, play-residental core model is learning by playing by self, and is equiped with acting, eating and reading common area clustering two or three group room. Thirdly, eco-residental core model is learning by eco-friendly activities, and is equiped with companying, cooperating and sharing area. Fourthly, project-residental core model is learning by project by self, and is equiped with drawing, experimenting and presenting common area. Fifthly, the space of residential core model is organized with three or four group room and clustering living or common area. The larger the center is, the more the cluster is vertically. Facility area and outdoor playground per child is about 7 and $3m^2$.