• Journal of Animal Reproduction and Biotechnology
• /
• v.37 no.2
• /
• pp.80-86
• /
• 2022

The ability to determine the sex of bovine embryos before the transfer is advantageous in livestock management, especially in dairy production, where female calves are preferred in milk industry. The milk production of female and male cattle benefits both the dairy and beef industries. Pre-implantation sexing of embryos also helps with embryo transfer success. There are two approaches for sexing bovine embryos in farm animals: invasive and non-invasive. A non-invasive method of embryo sexing retains the embryo's autonomy and, as a result, is less likely to impair the embryo's ability to move and implant successfully. There are lists of non-invasive embryo sexing such as; Detection of H-Y antigens, X-linked enzymes, and sexing based on embryo cleavage and development. Since it protects the embryo's autonomy, the non-invasive procedure is considered to be the safest. Invasive methods affect an embryo's integrity and are likely to damage the embryo's chances of successful transformation. There are different types of invasive methods such as polymerase chain reaction, detection of male chromatin Y chromosome-specific DNA probes, Loop-mediated isothermal amplification (LAMP), cytological karyotyping, and immunofluorescence (FISH). The PCR approach is highly sensitive, precise, and effective as compared to invasive methods of farm animal embryonic sexing. Invasive procedures, such as cytological karyotyping, have high accuracy but are impractical in the field due to embryonic effectiveness concerns. This technology can be applicable especially in the dairy and beef industry by producing female and male animals respectively. Enhancing selection accuracy and decreasing the multiple ovulation embryo transfer costs.

• Analytical Science and Technology
• /
• v.37 no.5
• /
• pp.271-279
• /
• 2024

Bisphenols and phthalates are endocrine-disrupting chemicals that are commonly used in packaging and as plasticizers. However, they pose health risks through ingestion, inhalation, and dermal contact. Accurate analysis of these pollutants is challenging owing to their low concentration and their presence in complex oil matrices. Therefore, they require efficient extraction and detection methods. In this study, an analytical method for the simultaneous quantification of bisphenols and phthalates in corn oil is developed. The dynamic multiple reaction monitoring mode of liquid chromatography-tandem mass spectrometry is used according to the different polarities of bisphenols and phthalates. The method is validated by assessing system suitability, linearity, accuracy, precision, homogeneity, and stability. The determination coefficients are higher than 0.99, which is acceptable. The percentage recovery and coefficient of variation of the accuracy and precision confirm that this analytical method is capable of simultaneously quantifying bisphenols and phthalates in corn oil. The bisphenols and phthalates in the formulations and pretreatment samples are stable for 7 d at room temperature and 24 h in an auto-sampler. Therefore, this validated analytical method is effective for the simultaneous quantification of bisphenols and phthalates in oils.

• Tuberculosis and Respiratory Diseases
• /
• v.78 no.1
• /
• pp.1-7
• /
• 2015

Background: The incidence of tuberculosis (TB) in Korea is relatively high compared to the other Organisation for Economic Co-operation and Development (OECD) countries, with a prevalence of 71 per 100,000 in 2012, although the incidence is declining. Real-time polymerase chain reaction (PCR) has been introduced for the rapid diagnosis of TB. Recently, its advantage lies in higher sensitivity and specificity for the diagnosis of TB. This study evaluated the clinical accuracy of real-time PCR using respiratory specimens in a clinical setting. Methods: Real-time PCR assays using sputum specimens and/or bronchoscopic aspirates from 2,877 subjects were reviewed retrospectively; 2,859 subjects were enrolled. The diagnosis of TB was determined by positive microbiology, pathological findings of TB in the lung and pleura, or clinical suspicion of active TB following anti-TB medication for more than 6 months with a favorable response. Results: Sensitivity, specificity, and accuracy were 44%, 99%, and 86% from sputum, and 65%, 97%, and 87% from bronchoscopic aspirates, respectively. For overall respiratory specimens, sensitivity was 59%, specificity was 98%, and accuracy increased to 89%. Conclusion: Positivity in real-time PCR using any respiratory specimens suggests the possibility of active TB in clinically suspected cases, guiding to start anti-TB medication. Real-time PCR from selective bronchoscopic aspirates enhances the diagnostic yield much more when added to sputum examination.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.31 no.1D
• /
• pp.65-72
• /
• 2011

When an expressway intersects another expressway, a unique connector needs to be designed between the two consecutive exit ramps. In such a case, it is important to design a connector such that there is enough distance for drivers to find their way safely. A current design manual in Korea prescribes the minimum length of the connector as 240 m. In this research, we have suggested a method for calculating the minimum length of a connector in order to check the feasibility of the currently prescribed length. For this purpose, we have attempted to determine the total perception-reaction time and lane-changing time required by a driver. For determining the driver's perception-reaction time, we have used the driver's decision time in addition to the conventional 2.5 s of perception-reaction time for stopping sight distances. We have considered both the design speed and the average travel speed for the calculation of the length. To evaluate the accuracy of the new method, we have chosen four sites on expressways for which relatively high accident rates were recorded. As a result, we could verify that the current limit (240 m) was sufficient for drivers to be able to change lanes in the given specific geometry. However, the prescribed limit should be revised in case the drivers' decision time is considered to be their perception-reaction time. All new approaches for calculating the ideal length of a connector have been carried out by taking into account the design speed as well as the average travel speed. Owing to the characteristics of the specific geometry for two consecutive exit ramps and the large difference between the design speed and the average travel speed in the objective areas, it is more realistic to use the proposed method by keeping the decision time equal to a driver's perception-reaction time, in order to determine the ideal distance that should be maintained between two consecutive exit ramps.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.40 no.9
• /
• pp.827-832
• /
• 2012

In this paper, we will design the speed measurement board of LEO Satellite's reaction wheel which has two speed measuring methods as M-Method type and T-Method type. therefore we can use the advantage of two methods. and we will verify the availability of design on the on-board computer at the real LEO Satellite(KOMPSAT-3). In the reaction wheels satellite that can change the satellite's attitude is one of the leading drivers by the rotational inertia of the motor will perform attitude control. Reaction methods for detecting wheel rotation speed generated during a certain period T internal reaction wheel tacho pulse counting M-Method to detect wheel speed and wheel tacho pulses are generated by measuring the time between the detection rate can be divided into T-Method. M-method is simple to implement and benefit measurement time is constant, but slow fall in the velocity measurement accuracy is a disadvantage. In contrast, the time between tacho pulses to measure the T-Method to measure the precise speed at low speed and to measure the time delay is small, has the advantage. However, this method also in the actual implementation and the complexity of the operation at different speeds depending on the speed of operation has the disadvantage.

• The Journal of the Korea Contents Association
• /
• v.18 no.5
• /
• pp.514-524
• /
• 2018

The purpose of this study was to investigate learners' reaction behaviors by the type of learning motive when teaching messages were delivered. The subjects of this study were 82 students from Auniversity and Buniversity in the metropolitan area. Students were online collaborative learning. The instructor delivered six messages. Since then, the researchers have measured the learner 's response time and the frequency and level of interaction. And analyzed using an independent sample t-test. As a result, the frequency and level of interaction increased before the message was delivered. The response time to instructional messages was the fastest among the students with performance avoidance goal orientation. Mastery goal orientation students were most sensitive to scaffolding messages. Performance avoidance goal orientation students responded most sensitively to the reminder message. Finally, Mastery goal orientation students had the most action on new topics. And performance approach goal orientation students had the most to do with accuracy and relevance.

• Journal of Advanced Marine Engineering and Technology
• /
• v.40 no.4
• /
• pp.288-294
• /
• 2016

In modern ships, the shafting system often becomes stiff owing to the high engine power, whereas the hull structure becomes more flexible owing to optimization using high-tensile-strength thick steel plates; therefore, more sophisticated shaft alignments are required. In this study, strain gauge-based measurement was conducted under five vessel operating conditions and bearing reaction forces and hull deflections affecting shaft alignment were analyzed for a 50,000 dead weight tonnage oil/chemical tanker that has gained repute as an eco-friendly vessel in recent years. Furthermore, the analytical results from each technique-theoretical calculation, jacking ups, and strain gauges-were cross-checked against each other in order to enhance the degree of accuracy and reliability of the calculation.

• Journal of Environmental Health Sciences
• /
• v.18 no.2
• /
• pp.82-91
• /
• 1992

The examination of the pollutants originated from domestic sewage, industrial and agricutural activities the existences of some toxic heavy metals, organic matters and pathogenic microorganisms. A recent report of WHO brought out that such pollutants are in existence with above roughly 2,000 kinds of chemical substances and amongst them about 750 chemicals have been indentified by drinking water. And above 600 kinds of them are organic pollutants and in addition these include carcinogenic mutagenic and poisonous substances. This is not intended to embody a study of broad confined to various approaches on organic materials, and therefore will be THM produced on injection of chlorine at water filtration plant. To specify the relations between THM and factors having an effect upon THM such as TOC, Cl$_{2}$, Temperature, pH and reaction time, first of all the recovery ratio for analytical methods of THM (Head sapce, purge and trap, Liquid/ Liquid Extraction methods) was investigated. Provided that by using it,the correction coefficients are obtained, the accuracy of data might be able to be enhanced through analysis.The result of the experiments are given in the followings. 1) Among three kinds of analytical methods, recovery rate was higher in order of purge and trap Liquid/Liquid Extraction, Head space. There is no great difference in recovery rate among three methods. 2) The higher the concentration of TOC, the more the amount of THM. 3) The higher the reaction temperature, the more the amount of THM. 4) The longer the reaction time, the more the amount of THM. 5) The higher the pH, the more the amount of THM. 6) The higher the concectration of chlorine, the more the amount of THM.

• Asian-Australasian Journal of Animal Sciences
• /
• v.28 no.4
• /
• pp.568-572
• /
• 2015

Acetes chinensis is an economically important shrimp that belongs to the Sergestidae family; following fermentation, A. chinensis' economic value, however, is low in China, and much of the catch in China is exported to Korea at a low price, thus leading to potential false labeling. For this reason, we developed a simple method to identify A. chinensis' origin using allele-specific polymerase chain reaction (PCR). Ten single nucleotide polymorphisms (SNPs) were identified from partial (i.e., 570 bp) DNA sequence analysis of the mitochondrial 16s rRNA gene in 96 Korean and 96 Chinese individual shrimp. Among 10 SNP sites, four sites were observed in populations from both countries, and two sites located in the middle with SNP sites at their 3'-ends were used to design allele-specific primers. Among the eight internal primers, the C220F primer specific to the Chinese A. chinensis population amplified a DNA fragment of 364 bp only from that population. We were able to identify the A. chinensis population origin with 100% accuracy using multiplex PCR performed with two external primers and C220F primers. These results show that the 16S rRNA gene that is generally used for the identification of species can be used for the identification of the origin within species of A. chinensis, which is an important finding for the fair trade of the species between Korea and China.

• Journal of Dental Anesthesia and Pain Medicine
• /
• v.20 no.5
• /
• pp.271-279
• /
• 2020

Background: The present study aimed to evaluate and compare the efficacy of buffered and unbuffered local anesthesia solutions during inferior alveolar nerve block (IANB) administration in children. Methods: PubMed, Ovid SP, and Cochrane databases were searched separately by two independent reviewers for potential papers published between 1980 and April 2020 using relevant MeSH terms and pre-specified inclusion and exclusion criteria. T Studies of IANB administration in children comparing buffered and unbuffered local anesthesia solutions were evaluated. The primary outcome evaluated was pain (perception and reaction), while the secondary outcome was the onset of anesthesia. Results: A total of five articles were included in a qualitative analysis; among them, four qualified for quantitative analysis of the primary outcome and three for quantitative analysis of the secondary outcome. A fixed-effects model was used to perform the meta-analysis. Pain perception (child-reported pain): Significantly lower pain scores were reported with buffered local anesthesia solution than with unbuffered solution (P = 0.006, MD: -0.32, 95% CI: -0.55 to -0.09). Pain reaction (observer-reported pain reaction in child): No significant difference was found between buffered and unbuffered solution in terms of observer-reported pain behavior in the child (P = 0.09, MD: -0.21, 95% CI: -0.46 to 0.04). Onset of anesthesia: A significantly lower duration of anesthesia onset was reported with buffered local anesthesia solution than with unbuffered solution (P = 0.00001, MD: -12.38, 95% CI: -17.64 to -7.13]. Conclusion: Buffering local anesthesia solution may reduce discomfort due to IANB injection administration and lower the initial onset time of anesthesia. More randomized control trials with adequate sample sizes should be carried out to validate the accuracy of these results.