• Journal of Microbiology and Biotechnology
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• v.31 no.1
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• pp.154-162
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• 2021

L-5-methyltetrahydrofolate (5-MTHF) is one of the biological active forms of folate, which is widely used as a nutraceutical. However, low yield and serious pollution associated with the chemical synthesis of 5-MTHF hampers its sustainable supply. In this study, 5-MTHF production was improved by engineering the 5-MTHF biosynthesis pathway and NADPH supply in Lactococcus lactis for developing a green and sustainable biosynthesis approach. Specifically, overexpressing the key rate-limiting enzyme methylenetetrahydrofolate reductase led to intracellular 5-MTHF accumulation, reaching 18 ㎍/l. Next, 5-MTHF synthesis was further enhanced by combinatorial overexpression of 5-MTHF synthesis pathway enzymes with methylenetetrahydrofolate reductase, resulting in 1.7-fold enhancement. The folate supply pathway was strengthened by expressing folE encoding GTP cyclohydrolase I, which increased 5-MTHF production 2.4-fold to 72 ㎍/l. Furthermore, glucose-6-phosphate dehydrogenase was overexpressed to improve the redox cofactor NADPH supply for 5-MTHF biosynthesis, which led to a 60% increase in intracellular NADPH and a 35% increase in 5-MTHF production (97 ㎍/l). To reduce formation of the by-product 5-formyltetrahydrofolate, overexpression of 5-formyltetrahydrofolate cyclo-ligase converted 5-formyltetrahydrofolate to 5,10-methyltetrahydrofolate, which enhanced the 5-MTHF titer to 132 ㎍/l. Finally, combinatorial addition of folate precursors to the fermentation medium boosted 5-MTHF production, reaching 300 ㎍/l. To the best of our knowledge, this titer is the highest achieved by L. lactis. This study lays the foundation for further engineering of L. lactis for efficient 5-MTHF biosynthesis.

• Clinics in Shoulder and Elbow
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• v.24 no.2
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• pp.88-92
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• 2021

Background: Patients who have rotator cuff arthropathy experience a limited range of motion (ROM) of the shoulder joint and experience problems in performing their daily activities; however, no evidence is available to suggest the exact ROM of the shoulder joint in this population. Therefore, this study sought to determine the degree of motion of the shoulder joint in three planes during different activities. Methods: Five subjects with rotator cuff injuries participated in this study. The motion of the shoulder joints on both the involved and normal sides was assessed by a motion analysis system while performing forward abduction (task 1), flexion (task 2), and forward flexion (task 3). The OpenSIM software program was used to determine the ROM of the shoulder joints on both sides. The difference between the ranges of motion was determined using a two-sample t-test. Results: The ROMs of the shoulder joint in task 1 were 93.5°±16.5°, 72.1°±2.6°, and 103.9°±25.7° for flexion, abduction, and rotation, respectively, on the normal side and 28°±19.8°, 31°±31.56°, and 48°±33.5° on the involved side (p<0.05). There was no significant difference between the flexion/extension and rotation movements of the shoulder joint when performing task 1. However, the difference between flexion and rotation movements of the shoulder joints for the second task was significant (p>0.05). Conclusions: Those with rotator cuff arthropathy have functional limitations due to muscle weakness and paralysis, especially during the vertical reaching task. However, although these individuals have decreased ROM for transverse reaching tasks, the reduction was not significant.

• Physical Therapy Korea
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• v.29 no.1
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• pp.70-78
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• 2022

Background: Individuals with calf muscle shortening may have decreased dynamic balance. Objects: This study aimed to investigate the effect of mobilization with movement (MWM) and myofascial release (MFR) on kinematic changes in dynamic balance in individuals with calf muscle shortening. Methods: Thirteen participants were randomly assigned to the MWM or the mobilization with movement added myofascial release (MWM-MFR) group. The MWM group received treatment with only MWM, whereas the MWM-MFR group was treated with MWM and MFR. Pre- and post-intervention passive range of motion (PROM), maximum reaching lengths, and modified star excursion balance test (MSEBT) results were compared for all participants. Wilcoxon signed-rank test and Mann-Whitney U test were used for statistical analysis. Results: The results showed significant within-group differences in ankle PROM, but no significant between-group differences. The maximum reaching length in the MWM-MFR group in the posterolateral direction was significantly different before and after the intervention (p = 0.005). This group also showed significantly reduced ankle abduction in MSEBT during the posteromedial direction section 3 (p = 0.007) and posterolateral direction section 5 (p = 0.049) compared with the MWM group. Conclusion: Combined MWM and MFR intervention improves ankle stability in the coronal plane during the posteromedial and posterolateral forward movement in dynamic balance compared with only MWM in individuals with calf shortening.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.1A
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• pp.15-26
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• 2010

Quasi-static tests were conducted to evaluate structural performance of precast piers prestressed by bonded prestressing steels. Combinations of prestressing bars and normal reinforcing bars, embedded steel tubes and prestressing strands were used as continuous steels crossing the joints of a precast pier. Main design parameters were steel ratio, magnitude of prestress force, and section details. Flexural strength and energy dissipation capacity of precast columns with higher steel ratio showed better performance due to continuous steels after opening of the joints. Precast piers with embedded members showed stable behavior after reaching maximum loads resulting in higher displacement ductility and energy dissipation capacity increased as the introduced prestress increased. Self-centering behavior at early stages and stress increase of confining reinforcements were observed from highly prestressed columns. Combination of prestressing steels and normal reinforcing bars should be used in design to prevent rapid strength degradation after reaching the maximum load.

• Korean Journal of Ichthyology
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• v.35 no.4
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• pp.244-252
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• 2023

This study compared the morphological and molecular characteristics of the two Pampus spp. in Korea, Japan and China to clarify the long-confused taxonomic status of the two Korean pomfrets named as "Byeong-eo" and "Deok-dae". Among them "Byeong-eo" was identified as Pampus punctatissimus in having the following morphological traits: patch of wavy ridges extending beyond the anterior tip of the pectoral fin, the groove on the lower ridge of gill cover absent, and the number of vertebrae 34. On the other hand, "Deok-dae" was identified as Pampus argenteus in having the following morphological traits: patch of wavy ridges not reaching to the anterior tip of the pectoral fin, the groove on the lower ridge of gill cover reaching to the lower jaw, and the number of vertebrae 38~42. In addition, "Byeong-eo" almost corresponded to P. punctatissimus from Japan in the mitochondrial DNA cytochrome c oxidase subunit I sequences (genetic distance=0.1%), while "Deokdae" showed genetic distance of 0.3% when compared to P. argenteus from China. Our comprehensive results suggest the validify of P. punctatissimus for "Byeong-eo", and P. argenteus for "Deok-dae".

• Journal of Environmental Science International
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• v.33 no.8
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• pp.591-604
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• 2024

To investigate the frequency and trajectories of volcanic ash from Mt. Baekdu reaching the Korean Peninsula, a forward trajectory analysis was conducted using the Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model. Through a cluster analysis of air parcel trajectories, we identified the main pathways of the volcanic ash from Mt. Baekdu entering the Korean Peninsula and analyzed the synoptic meteorological conditions on those days. The frequency of volcanic ash reaching the Korean Peninsula was 82 times at an altitude of 1000 m and 70 times at 2000 m, with an increasing trend from 2016 to 2022. This increase is attributed to the weakening of westerly winds and the strengthening of north-south winds due to global warming. Five and three trajectory clusters were classified at 1000 m and 2000 m, respectively. At a starting altitude of 1000 m, most air parcels originating from Mt. Baekdu entered the Korean Peninsula under weather conditions (C2, C3) where the pressure gradient from the northwest to the southeast was small, resulting in weak northerly winds. C2 and C3 showed shorter trajectories, which occurred in all seasons, except summer. At a starting altitude of 2000 m, air parcels mostly passed over the Korean Peninsula in a synoptic pattern similar to that at 1000 m in altitude; however, the air parcels had simpler paths and less frequent inflow. C2, at a starting altitude of 2000 m, originates from Mount Baekdu, crosses the center of the Korean Peninsula, and continues to the central region. At a starting altitude of 1000 m, volcanic ash can enter the Korean Peninsula when there is no strong low-pressure system to the southeast of the Korean Peninsula, whereas at 2000 m, volcanic ash can enter the Korean Peninsula when the Siberian high-pressure system is weak.

• Journal of Photoscience
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• v.9 no.2
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• pp.213-216
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• 2002

Skin pigmentation, also known as complexion coloration, results from the biosynthesis of melanin within the melanocytes of the Stratum basalum and the subsequent transfer, translocation, and degradation of this pigment to, in, and by the neighboring keratinocytes respectively, Melanins are produced and retained in melanosomes synthesized in the cell body that are translocated along the dendrites using microtubules via motor proteins. Melanosomes are eventually captured and retained at the tips of dendrites by attachment to the peripherally localized actin. Melanosomes reaching the dendritic tips are transferred to keratinocytes, primarily via phagocytosis of released melanosomes by keratinocytes. Molecules responsible for cell/cell recognition and interaction that regulate transfer are being identified. Some of these putative mediators appear to be affected by ultraviolet radiation. After the keratinocytes receive melanosomes, the granules are distributed individually or as clusters in dark versus light skin respectively. These melanosomes are then aggregated over the nucleus for photoprotection ofkeratinocyte DNA and eventually degraded.

• Journal of the Optical Society of Korea
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• v.7 no.3
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• pp.135-138
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• 2003

A chirped-pulse amplification femtosecond Ti:sapphire laser operating at 1 KHz has been developed. The laser system consisted of a long-cavity femtosecond oscillator, a four-pass grating pulse stretcher, two multi-pass amplifiers and a double-pass grating pulse compressor. Thermal lensing at the amplifiers was reduced by cooling Ti:sapphire crystals using Peltier coolers. Gain narrowing and residual phase errors were compensated for by the use of an acousto-optic pulse shaper. The final laser output had an energy per pulse of 2.0 mJ and a pulse duration of 19.5 fs, reaching 0.1 TW at 1 KHz.

• Structural Engineering and Mechanics
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• v.52 no.2
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• pp.405-426
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• 2014

In this paper, a new meta-heuristic algorithm named Ranked Particles Optimization (RPO), is presented. This algorithm is not inspired from natural or physical phenomena. However, it is based on numerous researches in the field of meta-heuristic optimization algorithms. In this algorithm, like other meta-heuristic algorithms, optimization process starts with by producing a population of random solutions, Particles, located in the feasible search space. In the next step, cost functions corresponding to all random particles are evaluated and some of those having minimum cost functions are stored. These particles are ranked and their weighted average is calculated and named Ranked Center. New solutions are produced by moving each particle along its previous motion, the ranked center, and the best particle found thus far. The robustness of this algorithm is verified by solving some mathematical and structural optimization problems. Simplicity of implementation and reaching to desired solution are two main characteristics of this algorithm.

• Proceedings of the KIEE Conference
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• 2002.07b
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• pp.1158-1160
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• 2002

In this paper, we presents sliding surface design techniques using the virtual state. This sliding surface has same dynamic of nominal system and permit application with other controller. If select initial value of the virtual state properly, problem of reaching phase disappears. And applied in AC servo motor's speed control to show performance of proposed sliding mode control techniques.