• Molecules and Cells
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• v.39 no.7
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• pp.573-579
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• 2016

During copulation, male Drosophila transfers Sex Peptide (SP) to females where it acts on internal sensory neurons expressing pickpocket (ppk). These neurons induce a post-mating response (PMR) that includes elevated egg-laying and refractoriness to re-mating. Exactly how ppk neurons regulate the different aspects of the PMR, however, remains unclear. Here, we identify a small subset of the ppk neurons which requires expression of a pre-mRNA splicing factor CG3542 for egg-laying, but not refractoriness to mating. We identify two CG3542-ppk expressing neurons that innervate the upper oviduct and appear to be responsible for normal egg-laying. Our results suggest specific subsets of the ppk neurons are responsible for each PMR component.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.5
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• pp.697-700
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• 2001

A prolonged farrowing interval is common on small farms in Nepal and may be attributable to the absence of boar contact at the time of weaning. To examine whether synthetic boar pheromone spray might be of value to aid the stimulation and detection of estrus, 30 village farms, each with a single sow, were recruited into a field study. All sows entered the study on final weaning from their first lactation at 56 days post partum. For 13 treatment sows, a boar pheromone spray (SOA, Intervet, Boxmeer, The Netherlands) was applied each day after weaning whilst 17 other sows did not receive any stimulus (negative control). When estrous was detected by the farmer, sows were taken to the nearest boar for service. There was a significantly shorter weaning to re-mating interval for treatment sows (42.9 V 82.2 day, p<0.05), with a significantly higher proportion of treatment sows rebred by day 60 after weaning (p=0.024, Fishers Test). It is concluded that use of boar pheromones can improve reproductive efficiency of sows kept under Nepalese village conditions.

• Journal of the Korean Society of Safety
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• v.21 no.3 s.75
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• pp.1-7
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• 2006

Nowadays, out of other transmission parts, the sleeve is getting more and more important part for exact and smooth shifting from gear ratio change whenever drivers are needed. To exact and smooth shifting when drivers are needed, all the parts connected with gear shifting should be machined exactly and having dimensions designers are intended. Especially, in case of the sleeve that the most important functional part to shift from gear ratio change that drivers are intended, it needs high precision grade and quality in both sides runout and outer dia runout as well as inner spline small dia & large dia. Because it's assembled with the synchro hub spline and shifted directly with the mating cone. So, it should be applied the hear treatment(hereinafter referred to H.T.M.T) to prevent the friction and percussion loss from shifting with mating cone. At this time, the deformation problems are raised from almost H.T.M.T. process and it makes the inferior part.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.5
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• pp.738-748
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• 2012

One of the main goals of small, medium and large farms is to improve the reproductive performance of rabbit does. Stocks of lower productivity can be improved by crossing with intensive breeds. A better nutritional status of both foetuses and suckling kits has a positive effect on their later productivity. Overfeeding young females before first mating can lead to conditions of fattiness. Using restricted feeding or higher fibre content in the feed and changing it for a higher level ad libitum feeding about one week prior to first mating leads to longer lifespan and higher productive level. Intensive reproductive rhythm creates a negative energy balance in does : they are unable to consume enough feed (energy) for the nutritional requirements of foetus and lactation, and therefore lose most of their fat reserves. Furthermore, primiparous does also expend energy because they are still growing. Under intensive conditions, the 42-d reproductive rhythm (re-mating 11 days after parturition) is recommended. Under extensive conditions, the 18 or 25-d mating interval with 35 to 42-d weaning could be suitable. On small farms, natural mating is favoured; on large farms AI is commonly employed. The main advantage of AI is the all-in, all-out system. Hormonal (PMSG) treatment is used with AI to increase receptivity on d 11. Frequent and high level PMSG use can lead to higher anti-PMSG antibody rates. Lower level (max. 20 IU) and less frequent PMSG injection or non-hormonal alternative methods (short dam-litter separation, changing nursing method or lighting programs) are recommended for this reason.

• Journal of Microbiology
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• v.34 no.1
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• pp.30-36
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• 1996

We have exmained the re-establishment of HIMRE mediated silencing function on the transcriptional activity of yeast heast shock gene HSP82. To test whether the onset of SIR repression can occur in growing cells in the rpesence of a potent inhibitor of DNA replication, HMRa/HSP82 strains with SIR4- and SIR4S$^{+}$ genetic backgrounds were arrested in S phase by incubation of a culture in 200 mM hydroxyurea for 120 min. It was clear that following a 20 minute heat shock, silencing of the HMRa/HSP82 allele in cells pretreated with hydroxyurea does occur in a SIR4-dependen fashion, even though the kinetics of repression appears to be substantially delayed. We also have tested whether re- establishement of silencing at the HMR/hsp82 locus can occur in G1-arrested cells. Cell cycle arrest at G1 phase was achieved by treatment of early log a cell cultures with .alpha.-factor mating pheromone, which induces G1 arrest. The result suggests that passage through S phase (and therefore DNA replication) is nor required for re-establishing silencer-mediated repression at the HMNRa/HSP82 locus. Finally, to test whether de nono protein synthesis is required for re-establishment of silencer-mediated repression, cells were pretreated with cycloheximide (500 /.mu.g/ml) 120 min. It was apparent that inhibiting protein synthesis delays, but does not prevent, re-establishment of silencer-mediated repression. Altogether, these results indicate that re-establishment of silencer-mediated repression is not dependent on the DNA replication and has no requirement for protein synthesis.s.

• Korean journal of applied entomology
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• v.45 no.3 s.144
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• pp.283-291
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• 2006

The efficiency to detect mutagenicity of the system using a specific locus mutation of Bombyx mori was examined and improved. In the system, mutagenicity could be detected by the egg colour manifested by the pe and/or re genes, which is a kind of recessive visible mutation of the insect. Among tested four mutagens, MMC had specially high sensitivity in the oocytes of silkworm and EMS had in the spermatozoa. PCB and dioxin showed a positive effect in both the oocytes and spermatozoa. In a consequence of sensitivity of mutagen by mating number of male moth of B. mori, treated mutagen, there was no difference between one mating - and three mating - male moth in sensitivity of mutagen. Sun3ho, B. mori variety, which showed high sensitivity to mutagens was improved in the major characteristics by crossing of C5 and N12.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.2
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• pp.115-121
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• 2002

To develop a new method of conjugation and to determine the distribution of R plasimds, we isolated multi-drug resistant strains from fish pathogenic bacteria in the farms of south and east seacoasts of Korea. Out of the 134 isolates examined, 10 showed resistance to chloramphenicol, tetracycline, streptomycin, ampicillin, colistin, nalidixic acid, oxolinic acid and kanamycin. One out of 10 multi-drug resistance bacteria, Vibfio damsela JE1 (V. damsela JE1), contained transferable R plasmid of chlorarnphenicol- tetracycline resistance genes and other nucleic acids encoding ampicillin and kanamycin resistance. The presence of the R plasmid was confirmed by conjugation using the chromocult medium (CC) as a selective and differential medium for transconiugants with identification based on the growth or colors of the colonies. The frequency of R plasmid transfer with filter mating method was come out much higher than that of broth mating method and appeared to be dependent upon the mating time and temperature. The optimum conditions for filter mating method were found to be 30$^{\circ}C$ and 24hrs as mating temperature and period, respectively, Moreover, donor cells with R plasmid, both isolate and standard bacteria, were shown to have an ability to transfer the plasmid against Escherichia coli K-12 HB101 (E. coli HB101) and Edwardsiella tarda (E. tarda) RE14 at fairly high frequencies, finally, we isolated 3 isolates of Sphingomonas sp., carrying R plasmid from 12 multi-drug resistant bacteria in normal microflora of the flounder (Paralichthys olivaceus) group used for the isolation of V emsela JE1 four months before. The same size and gene transfer chayateristics of R plasimds with those of V damsela JE1 confirmed that normal microflora have the reservoir activity for R plasmid in natural aquatic environment.

• Journal of Microbiology
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• v.34 no.1
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• pp.37-37
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• 1996

The internal regions of nuclear small subunit rRNA from 6 plaeurotus species and 5 Pleurotus ostreatus strains were amplified by PCR and sequenced. The DNA sequences of 8 Pleurotus strains (P. ostreatus NFFA2, NFFA4501, NFFA4001, KFFA4001, KFCC11635, P florida, P. florida, P. sajor-cuju, P. pulmonarius, and P. spodoleucus) were idential, but P. cornucopiae differed from them in two bases out of 605 bases. However, p[hylogenetic analysis of the sequences by DNA-distance matrix and UPGMA methods showed that P. ostreatus NFFA2m1 and NFFA2m2, known as mutants of P. ostreatus NFFA2, belonged to anther group of Basidiomycotina, which is close to the genus Auricularia. The difference of the SSU rDNA sequences of P. cornucopiae from other Pleurotus species tested corresponds to the difference of mitochondrial plasmid type present in Pleurotus species as observed by Kim et al. (1993, Korean J. Microbiol. 31, 141-147).ishement of silencing at the HMR/hsp82 locus can occur in G1-arrested cells. Cell cycle arrest at G1 phase was achieved by treatment of early log a cell cultures with .alpha.-factor mating pheromone, which induces G1 arrest. The result suggests that passage through S phase (and therefore DNA replication) is nor required for re-establishing silencer-mediated repression at the HMNRa/HSP82 locus. Finally, to test whether de nono protein synthesis is required for re-establishment of silencer-mediated repression, cells were pretreated with cycloheximide (500 /.mu.g/ml) 120 min. It was apparent that inhibiting protein synthesis delays, but does not prevent, re-establishment of silencer-mediated repression. Altogether, these results indicate that re-establishment of silencer-mediated repression is not dependent on the DNA replication and has no requirement for protein synthesis.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2002.10b
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• pp.305-306
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• 2002

In this paper, the endurance life of the rolling bearings with defective balls and their failure (flaking) phenomena are presented. It was found that the lives of ball bearings with defective balls were shorter than that of calculated L10 life as well as that of normal bearings in spite of the using standard bearing components. Although the bearings were assembled with defective balls, whereas the other parts were qualified new ones, the main failures were occurred on the inner ring raceways. Moreover, the failures were on the center of the groove curvature and the severity of failure is similar to the order of initial defect depth of the balls. These shows that the defects on the bearings can affect the life of tribologically contacted mating parts.

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.81-81
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• 2002

The deer was inseminated at the breeding season, which only shows appearance of the estrus cycles. Early diagnosis of pregnancy is very useful to select non-pregnant does and also very important to decide re-inseminations or mating for producing of calves. Therefore, for the development of techniques on the early diagnosis of pregnancy of deer, the results of this study were summarized as following. (omitted)