• Title/Summary/Keyword: Raw oyster

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Anti-inflammatory Effect of Oyster Shell Extract in LPS-stimulated Raw 264.7 Cells

  • Lee, Se-Young;Kim, Hak-Ju;Han, Ji-Sook
    • Preventive Nutrition and Food Science
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    • v.18 no.1
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    • pp.23-29
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    • 2013
  • This study was designed to investigate the anti-inflammatory effect of oyster shell extract on the production of pro-inflammatory factors [NO, reactive oxygen species (ROS), nuclear factor-kappa B (NF-${\kappa}B$), inducible nitric oxide synthase (iNOS) and cycloxygenase-2 (COX-2)] and pro-inflammatory cytokines [Interleukin-$1{\beta}$ (IL-$1{\beta}$), Interleukin-6 (IL-6) and TNF-${\alpha}$] in the lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. Cell viability, as measured by the MTT assay, showed that oyster shell extract had no significant cytotoxicity in Raw 264.7 cells. The treatment with oyster shell extract decreased the generation of intracellular reactive oxygen species dose dependently and increased antioxidant enzyme activities, such as SOD, catalase, GSH-px in LPS-stimulated macrophage cells. Oyster shell extract significantly suppressed the production of NO and also decreased the expressions of iNOS, COX-2 and NF-${\kappa}B$. Additionally, oyster shell extract significantly inhibited the production of IL-$1{\beta}$, IL-6, and TNF-${\alpha}$ in LPS-stimulated Raw 264.7 cells. Thus, these results showed that the oyster shell extract had an anti-inflammatory effect on LPS-stimulated Raw 264.7 cells.

Analysis of Volatile Flavor Compounds in Raw Oyster and Oyster Cooking Drips by Gamma Irradiation Using Headspace Method (Headspace 법을 이용한 생굴 및 굴 자숙액의 감마선 조사에 의한 휘발성 냄새 성분 변화 분석)

  • Choi, Jong-Il;Kim, Hyun-Joo;Lee, Ju-Woon
    • KSBB Journal
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    • v.26 no.2
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    • pp.177-181
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    • 2011
  • This study was conducted to investigate the effects of gamma irradiation on the change of volatile flavor compounds of raw oyster and its cooking drips using headspace methods. Major volatile flavor compounds of the raw oyster were identified as methylthiomethane and 1,5-hexadiene. When the raw oyster was irradiated at the dose of 5 kGy, 1-pentane was newly detected. On the other hand, 9 compounds including N-methoxyformaldehyde were identified as the major volatile compounds of cooking drips from oyster. Among them, N-methoxyformaldehyde contents in cooking drip was decreased by the gamma irradiation. By the gamma irradiation above 30 kGy, new heterocyclic compounds was found in oyster cooking drips. Therefore, the amount of volatile flavor compounds in the raw oyster and cooking drips were changed by gamma irradiation, and these results could be potentially used in the seasoning industry.

Change in Flavor Patterns of Gamma Irradiated Raw Oyster and Oyster Cooking Drip Determined using an Electric Nose (전자코에 의한 감마선조사 생굴과 굴자숙액의 향기패턴 변화)

  • Han, In-Jun;Park, Jae-Seok;Choi, Jong-Il;Kim, Jae-Hun;Song, Beom-Seok;Yoon, Yo-Han;Byun, Myung-Woo;Chun, Soon-Sil;Lee, Ju-Woon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.3
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    • pp.209-214
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    • 2009
  • This study was conducted to evaluate changes in the flavor patterns of gamma irradiated raw oyster and oyster cooking drip using an electronic nose, which consisted of a GC equipped with a surface acoustic wave sensor. The raw oyster was irradiated with 1, 2, 3, 4 and 5 kGy, while the oyster cooking drip was irradiated with 10 and 50 kGy. In the case of raw oyster, the intensities of peaks at retention times (RT) of 2.1 sand 6.8 s were increased, but the peak at a RT of 9.0 s was decreased depending on irradiation dose. In the case of oyster cooking drip, the intensities of peaks at RT 2.5 sand RT 4.1 s increased linearly, but at the peak at RT 5.1 s decreased as the irradiation dose increased. The total amount of flavor components measured in raw oyster increased, while that of oyster cooking drip decreased in response to irradiation.

A Study on Grading According to Raw Materialization of Oyster shell (굴 패각의 원료화 공정에 따른 입도분포에 관한 연구)

  • Jung, Ui-In;Hong, Sang-Hun;Choi, In-Kwon;Kim, Bong-Joo;Won, Chul-Hee;Choi, Ho-Rim
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2016.05a
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    • pp.195-196
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    • 2016
  • Oyster shell is produce by shucking process in oyster farming in southern coast of Korea. In average, about 6.7kg of oyster shell is produced as an industrial waste for 1kg of oyster flesh, and even only in last year, it is estimated that about 150,000 ton of oyster shell is produced. Oyster shell is light weighted and the strength characteristic of it is similar to send. So we would like to test if it is avail to use oyster shell crust as fine aggregate. So we use a crusher to crush oyster shell and review its particle distribution for materialization.

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Volatile Compounds in Oyster Hydrolysate Produced by Commercial Protease

  • Cha, Yong-Jun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.3
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    • pp.420-426
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    • 1995
  • Volatile compounds in raw oyster and oyster hydrolysate produced with protease were compared by vacuum simultaneous steam distillation-solvent extraction/gas chromatography/mass spectrometry. Sixty-two volatile compounds were detected in both samples. Of these, 57 were positively identified, composed mainly of aldehydes(12), ketones(9), alcohols(14), nitrogen-containing compounds(9), acids(6), terpenes(4), and miscellneous compounds(8). Levels of acids decreased after hydrolysis, whereas several other compounds such as aldehydes, ketones, and nitrogen containing compounds increased. Pyrazines, found in high abundance, were only detected in oyster hydrolysate.

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Microbial Risk Assessment of High Risk Vibrio Foodborne Illness Through Raw Oyster Consumption (생굴 섭취로 인한 고병원성 Vibrio균 식중독 위해평가)

  • Ha, Jimyeong;Lee, Jeeyeon;Oh, Hyemin;Shin, Il-Shik;Kim, Young-Mog;Park, Kwon-Sam;Yoon, Yohan
    • Journal of Food Hygiene and Safety
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    • v.35 no.1
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    • pp.37-44
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    • 2020
  • This study investigated the probability of foodborne illness caused by raw oyster consumption contaminated with high risk Vibrio species such as V. vulnificus and V. cholerae. Eighty-eight raw oyster samples were collected from the south coast, west coast and Seoul areas, and examined for the prevalence of high risk Vibrio species. The growth patterns of V. vulnificus and V. cholerae in raw oysters were evaluated, and consumption frequency and amounts for raw oyster were investigated from a Korean National Health and Nutrition Examination Survey. With the collected data, a risk assessment simulation was conducted to estimate the probability of foodborne illness caused by intake of raw oysters, using @RISK. Of 88 raw oysters, there were no V. vulnificus- or V. cholerae-positive samples. Thus, initial contamination levels of Vibrio species in raw oysters were estimated by the statistical methods developed by Vose and Sanaa, and the estimated value for the both Vibrio spp. was -3.6 Log CFU/g. In raw oyster, cell counts of V. vulnificus and V. cholerae remained unchanged. The incidence of raw oyster consumers was 0.35%, and the appropriate probabilistic distribution for the consumption amounts was the exponential distribution. A risk assessment simulation model was developed with the collected data, and the probability of the foodborne illness caused by the consumption of raw oyster was 9.08×10-15 for V. vulnificus and 8.16×10-13 for V. cholerae. Consumption frequency was the first factor, influencing the probability of foodborne illness.

A Study on Strength Properties of Mortar According to Mass ratio change using Oyster shell as Aggregate (굴 패각을 잔골재로 사용한 모르타르의 질량비 변화에 따른 강도특성에 관한 연구)

  • Jung, Ui-In;Yu, Nam-Gyu;Hong, Sang-Hun;Kim, Bong-Joo;Won, Chul-Hee;Choi, Ho-Rim
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2016.10a
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    • pp.156-157
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    • 2016
  • Oyster shell is produced by shucking process in oyster farming in southern coast of Korea. In average, about 6.7kg of oyster shell is produced as an industrial waste for 1kg of oyster flesh, and even only in last year, it is estimated that about 150,000 ton of oyster shell is produced. Oyster shell is light weighted and the strength characteristic of it is similar to sand. So we produced mortar test using different law of multiple proportions of grounded oyster shell powder of its particle size according to fine aggregate standard and reviewed strength Properties.

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The Use of Oyster Shell Powders for Water Quality Improvement of Lakes by Algal Blooms Removal

  • Huh, Jae-Hoon;Choi, Young-Hoon;Lee, Hyun-Jae;Choi, Woo Jeong;Ramakrishna, Chilakala;Lee, Hyoung-Woo;Lee, Shin-Haeng;Ahn, Ji-Whan
    • Journal of the Korean Ceramic Society
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    • v.53 no.1
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    • pp.1-6
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    • 2016
  • In this year, Koreans have a shortage in agricultural and drinking water due to severe algal blooms generated in major lakes. Waste oyster shells were obtained from temporary storage near the workplace at which oysters were separated from their shells. Heating ($1000^{\circ}C$ for 1 h in air) was employed to convert raw oyster shell powders into calcium oxide powders that reacted efficiently with phosphorus and nitrogen to remove algal blooms from eutrophicated wastewater. As the dispersed amount of heated oyster shell powders was increased, water clarity and visual light penetration were improved. Coagulation, precipitation and carbonation process of the heated oyster shell powders in a water purifier facilitated removal of eutrophication nutrient such as phosphorus and nitrogen, which is both beneficial and economically viable. $CO_2$ implantation by carbonation treatment not only produced thermodynamically stable CaO in oyster shells to derive precipitated calcium carbonate (PCC) but also accelerated algal removal by activation of coagulation and precipitation process. The use of oyster shell powders led to a mean reduction of 97% in total phosphate (T-P), a mean reduction of 91% in total nitrogen (T-N) and a maximum reduction of 51% in chemical oxygen demand (COD), compared with the total pollutant load of raw algal solution. Remarkable water quality improvement of algal removal by heated oyster shell powders and PCC carbonation treatment will allow utilization as water resources to agricultural or industrial use.

Strength and Fire Resistance Characteristics of Oyster Shell Aggregate with Increasing Mass Ratio (굴 패각 골재의 질량비 증가에 따른 강도 및 내화특성)

  • Hong, Snag-Hun;You, Nam Gyu;Jung, Ui-In;Kim, Bong-Joo
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2017.11a
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    • pp.26-27
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    • 2017
  • Oyster packs generate about 150,000 tons a year. Various studies are under way to utilize this oyster shell. Ca is the main component of oyster shell and is used as a raw material of refractory board. Studies on application of refractory board using oyster shell are also continuing. It is expected that the refractory characteristics will be improved as the mass of oyster shell, that is Ca, increases. In this study, mortar specimens and board specimens were fabricated by increasing the mass ratio of oyster shells classified below 0.6mm, 1.2 ~ 0.6mm, 2.5 ~ 1.2mm and 5.0 ~ 2.5mm, and the strength and fire resistance characteristics were examined.

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Risk assessment for norovirus foodborne illness by raw oyster (Ostreidae) consumption and economic burden in Korea

  • Yoo, Yoonjeong;Oh, Hyemin;Lee, Yewon;Sung, Miseon;Hwang, Jeongeun;Zhao, Ziwei;Park, Sunho;Choi, Changsun;Yoon, Yohan
    • Fisheries and Aquatic Sciences
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    • v.25 no.5
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    • pp.287-297
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    • 2022
  • The objective of this study was to evaluate the probability of norovirus foodborne illness by raw oyster consumption. One hundred fifty-six oyster samples were collected to examine the norovirus prevalence. The oyster samples were inoculated with murine norovirus and stored at 4℃-25℃. A plaque assay determined norovirus titers. The norovirus titers were fitted with the Baranyi model to calculate shoulder period (h) and death rate (Log PFU/g/h). These kinetic parameters were fitted to a polynomial model as a function of temperature. Distribution temperature and time were surveyed, and consumption data were surveyed. A dose-response model was also searched through literature. The simulation model was prepared with these data in @RISK to estimate the probability of norovirus foodborne. One sample of 156 samples was norovirus positive. Thus, the initial contamination level was estimated by the Beta distribution (2, 156), and the level was -5.3 Log PFU/g. The developed predictive models showed that the norovirus titers decreased in oysters under the storage conditions simulated with the Uniform distribution (0.325, 1.643) for time and the Pert distribution (10, 18, 25) for temperature. Consumption ratio of raw oyster was 0.98%, and average consumption amount was 1.82 g, calculated by the Pert distribution [Pert {1.8200, 1.8200, 335.30, Truncate (0, 236.8)}]. 1F1 hypergeometric dose-response model [1 - (1 + 2.55 × 10-3 × dose)-0.086] was appropriate to evaluate dose-response. The simulation showed that the probability of norovirus foodborne illness by raw oyster consumption was 5.90 × 10-10 per person per day. The annual socioeconomic cost of consuming raw oysters contaminated with norovirus was not very high.