• Journal of Microbiology and Biotechnology
• /
• v.16 no.7
• /
• pp.1132-1138
• /
• 2006

Three types of xylanases (EC 3.2.1.8) were detected in the strain Aspergillus niger A-25, one of which, designated as XynIII, also displayed ${\beta}-(l,3-1,4)-glucanase$ (EC 3.2.1.73) activity, as determined by a zymogram analysis. XynIII was purified by ultrafiltration and ion-exchange chromatography methods. Its apparent molecular weight was about 27.9 kDa, as estimated by SDS-PAGE. The purified XynIII could hydrolyze birchwood xylan, oat spelt xylan, lichenin, and barley ${\beta}-glucan$, but not CMC, avicel cellulose, or soluble starch under the assay conditions in this study. The xylanase and ${\beta}-(l,3-1,4)-glucanase$ activities of XynIII both had a similar optimal pH and pH stability, as well as a similar optimal temperature and temperature stability. Moreover, the effects of metal ions on the two enzymatic activities were also similar. The overall hydrolytic rates of XynIII in different mixtures of xylan and lichenin coincided with those calculated using the Michaelis-Menten model when assuming the two substrates were competing for the same active site in the enzyme. Accordingly, the results indicated that XynIII is a novel bifunctional enzyme and its xylanase and ${\beta}-(l,3-1,4)-glucanase$ activities are catalyzed by the same active center.

• Journal of the Korean Society of School Health
• /
• v.10 no.2
• /
• pp.169-178
• /
• 1997

In order to investigate the complaint rates of subjective fatigue symptoms and study the relationship between them and body type, health awareness in middle school students, we carried out a survey of 1,209 students (654 boy students and 555 girl students) at a middle school in Taejon City by a self-recorded questionnaire. The survey was conducted in June, 1997. The results obtained were as follows: 1. Among complaints of fatigue, "feel like lying down" was the highest, followed by "feel anxious about things", "feel drowsy", "yawning a lot", "eye strain" and "whole body feels tired" in descending order. 2. In the average weighted score of fatigue complaint, dullness and sleepiness (I) was the highest, followed by a difficulty in concentration (II) and bodily projection of fatigue (III) in descending order. Fatigue seems to create mental stress rather than physical burdens. 3. In the classification of body type based on the $R\ddot{o}hrer$ index, the obesity type (145 and above) was 11.0%, standard type (110~144) was 64.7% and leptosomic type (l09 and below) was 24.3%. Comparison between both sexes revealed that the rate of obesity and leptosomic types were significantly higher in boy students than in girl students. 4. In terms body type awareness, the statistics are the following: those who consider themselves to be standard type, 44.3%, slightly obese 24.5%, leptosomic, 23.5% and obese 7.8%. However, the level of obesity awareness was significantly higher in girl students than in boy students. 5. As for health awareness, 36.5% considered themselves healthy, 5.4% generally healthy and 10.1% a little unhealthy, but the level of unhealthy awareness was higher in girl students than in boy students. 6. In the relationship between body type and health awareness, most students thought that they were obese in spite of their standard body type. Also, many students who were obese and leptosomic tended to consider themselves to be a little unhealthy. 7. The more the students were obese or felt unhealthy, the more the average weighted score of fatigue complaints was high.

• Nuclear Engineering and Technology
• /
• v.49 no.4
• /
• pp.744-751
• /
• 2017

The fatigue crack propagation behaviors of Z3CN20.09M duplex stainless steel (DSS) were investigated by studying oxide films of specimens tested in $290^{\circ}C$ water and air. The results indicate that a full oxide film that consisted of oxides and hydroxides was formed in $290^{\circ}C$ water. By contrast, only a half-baked oxide film consisting of oxides was formed in $290^{\circ}C$ air. Both environments are able to deteriorate the elastic modulus and hardness of the oxide films, especially the $290^{\circ}C$ water. The fatigue lives of the specimens tested in $290^{\circ}C$ air were about twice of those tested in $290^{\circ}C$ water at all strain amplitudes. Moreover, the crack propagation rates of the specimen tested in $290^{\circ}C$ water were confirmed to be faster than those tested in $290^{\circ}C$ air, which was thought to be due to the deteriorative strength of the oxide films induced by the mutual promotion of oxidation and crack propagation at the crack tip. It is noteworthy that the crack propagation can be postponed by the ferrite phase in the DSS, especially when the specimens were tested in $290^{\circ}C$ water.

• The Korean Journal of Ecology
• /
• v.15 no.2
• /
• pp.137-145
• /
• 1992

When exposed to 1, 700 ml of 0.089, 0.445 or 0.890 mM/l cadmius solution, dock (rumex crispus L)plants from heavily polluted chungrangchon site absorbed 0.0404, 0.2244 or 0.4929 mM of cadmium per g dw during the first 4 hours, which were 5.0~30.8 times faster uptake rate than those of water hyacinth (eichhornia crassipes Solm. - laub). Zinc, with which cadmium generally occurs together, did not affect the uptake rate in the concentration range of 0.0306 ~0.0918 mM/l. rumex cripus from chungrangchon site and from less polluted shingalchon site, when exposed to 1, 700 ml of 0.089 mM/l solution for 3 days, removed 68.4% and 63.2%, repectively, of the cadmium initially present in the solution. And when exposed to cadmium solution of the same concentration for the second time after cadmium-free hydroponic culture for three days, these plants removed 76.1% and 66.8% of cadmium, respectively. These removal rates were not significantly different between collection sites or between exposures, but were about 2 times greater for the first exposure, and 2~5 times greater for the second exposure, than those of water hyacinth. these results incate that rumex cripus can absorb, and thus remove, large amount of cadmium ion in wastewater, and so can be used in wastewater treatment, e.g. soil trench method. since there was large difference among the plants in the ability to absorb cadmium, it should be possible to select for strain with greater ability.

• Journal of Korean Society of Steel Construction
• /
• v.11 no.1 s.38
• /
• pp.33-42
• /
• 1999

In the present study, crack initiation criteria, static failure and tensile mode fatigue behavior for a rail steel are evaluated to assure the railway vehicle's safety. The transverse fissure, which is the most critical damage in the rail, is initiated by the maximum shear stress and its location is subsurface. In addition, the possibility of transition from the shear mode to the mixed mode increases with increasing the length of subsurface crack. Because of the brittleness by the welding, the fracture toughness of the welded part is lower than of the base metal. For low ${\Delta}K$, the stage II fatigue crack growth rates of the welded part is slower than of the base metal but, for high ${\Delta}K$, this different behavior for fatigue crack growth rate is nearly diminished. These trends are more remarkable for low stress ratio, R=0.1. It is believed that this behavior is caused by the change of the microstructure which that of the welded part is coarser than of base metal.

• Proceedings of the KOSOMBE Conference
• /
• v.1995 no.05
• /
• pp.27-33
• /
• 1995

A time-dependent large deformation fracture theory is developed for application to soft biological tissues. The theory uses the quasilinear viscoelastic theory of Fung, and particularizes it to constitutive assumptions on polyvinyl-chloride (PVC) (Part I) and cartilage (Part II). This constitutive theory is used in a general viscoelastic theory by Christensen and Naghdi and an energy balance to develop an expression for the fracture toughness of the materials. Experimental methods are developed for measuring the required constitutive parameters and fracture data for the materials. Elastic stress and reduced relaxation functions were determined using tensile and shear tests at high loading rates with rise times of 25-30 msec, and test times of 150 sec. The developed method was validated, using an engineering material, PVC to separate the error in the testing method from the inherent variation of the biological tissues. It was found that the the proposed constitutive modeling can predict the nonlinear stress-strain and the time-dependent behavior of the material. As an approximation method, a pseudo-elastic theory using the J-integral concept, assuming that the material is a time-independent large deformation elastic material, was also developed and compared with the time-dependent fracture theory. For PVC. the predicted fracture toughness is $1.2{\pm}0.41$ and $1.5{\pm}0.23\;kN/m$ for the time-dependent theory and the pseudo-elastic theory, respectively. The methods should be of value in quantifying fracture properties of soft biological tissues. In Part II, an application of the developed method to a biological soft tissue was made by using bovine humeral articular cartilage.

• Proceedings of the KSR Conference
• /
• 2011.10a
• /
• pp.261-267
• /
• 2011

The test fit has commonly used for the evaluation of the railbed condition, and indirect methods by using the compressional wave are also studied. the direct evaluation method by penetration test has not been studied. For the measurement of in-situ cone tip resistance of the railbed with minimizing the disturbance of the upper railbed. the cone penetrometer with the helical type outer rod(CPH) was developed. The outer rod, which has helical screw, is penetrated through the gravel layer and provides the reaction force for cone penetration testing. the cone tip resistances are measured by the mini cone penetrometer, where diameter is 15mm. For the developing the mini cone, strain gauge installation, circuit configuration, penetration rates and calibration process are considered. For the easy penetration of the screw rod in the field, the reaction force stepping plate and guide column are arranged. The screw rod are penetrated through the gravel layer. And the mini cone was pushed into the subgrade railbed at the penetration rate of 1mm/sec. The penetration test shows that the cone tip resistance increases along the depth. In addition, the subgrade condition is evaluated. This study demonstrates that the CPH may be effectively used for the evaluation of subgrade method any damage of the gravel layer.

• Journal of Microbiology
• /
• v.42 no.3
• /
• pp.169-173
• /
• 2004

Enterobacter cloacae (strain PR2/7), a genetically modified endophyte(GME) in citrus plants, carrying different plasmids (pEC3.0/18, pCelE, pEglA and pGFP), was inoculated into Citrus sinensis seedlings under greenhouse conditions. The impact of this on the indigenous bacterial endophytic community was studied by analyses of 2 different morphologic groups. The germination rates of inoculated seeds were evaluated in greenhouse, and plasmid stability under in vitro conditions. Results demonstrated a great and diverse endophytic community inside plants, and specialization in tissue colonization by some bacterial groups, in different treatments. Shifts in seed germination rate were observed among treatments: in general, the PR2/7 harboring pEglA bacterial- clone significantly reduced seed germination, compared to the PR2/7 harboring pEC3.0/18 clone. This suggests that the presence of the pEglA plasmid changes bacteria-seed interactions. The endophytic community of citrus seedlings changed according to treatment. In seedlings treated with the PR2/7 with pEglA clone, the population of group II decreased significantly, within the context of the total endophytic community. These results indicate that the application of GMEs induces shifts in the endophytic bacterial community of citrus seedlings.

• Fisheries and Aquatic Sciences
• /
• v.6 no.2
• /
• pp.66-73
• /
• 2003

To understand the physiology of a suspension-feeding bivalve and its potential impacts on the dynamics of red tides on tidal flats, rates of clearance and ingestion of Glauconome chinensis were measured as a function of algal concentration, when the bivalve was fed on a nontoxic strain of red tide dinoflagellate Prorocentrum minimum, Cochlodinium polykrikoides or Scrippsiella trochoidea. With increasing algal concentration, weight-specific clearance rate increased rapidly at lower concentrations and after reaching the maximum at ca. 0.2 to 1.0 mgC/L, it decreased at higher concentrations. Maximum clearance rate was nearly equal for different algal species and ranged between 2.1 and 2.6 L/g/hr. Weight-specific ingestion rate also increased at lower algal concentrations but saturated at higher concentrations. Maximum ingestion rate was 2 to 10 fold different with different algal species: S. trochoidea (10.1 mgC/g/hr), P. minimum (3.9 mgC/g/hr), and C. polykrikoides (0.99 mgC/g/hr). Nitrogen and protein content showed that S. trochoidea is the best among the tested three red tide dinoflagellates. The maximum filtration capacity, calculated by combining the data on ingestion rate from laboratory experiments and those from the field for the density of the bivalve and the red tide dinoflagellates was 4.7, 1.4, and 25.3 tons/m2/day for P. minimum, C. polykrikoides, and S. trochoidea, respectively. It is hypothesized that the abundant suspension-feeding bivalves in tidal flats can effectively mitigate the outbreak of red tides.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.41 no.6
• /
• pp.637-644
• /
• 2021

Dynamic response of structures can be evaluated experimentally by conducting cyclic loading tests. It has been known that steel materials are rate-dependent and the lateral response of a structure is significantly affected by the presence of axial force. However, the rate-dependency of steel column structures subjected to both axial and lateral loads has not been sufficiently studied yet due to the difficulty of controlling the axial force in a real-time manner during test. This study introduces an advanced way to apply the axial load in real-time to a column specimen using the adaptive time series (ATS) compensator and the flexible loading beam (FLB), where the H-shape steel columns made of SS275 are used for monotonic and cyclic loading tests with various loading rates with axial loads. The lateral strength and post-yield response of the steel columns are compared for each of monotonic and cyclic loading tests. The estimating equation of yield stress of various strain rate has proposed and finite element analysis were performed for comparison.