Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Impact of Genetically Modified Enterobacter cloacae on Indigenous Endophytic Community of Citrus sinensis Seedlings

  • Fernando Dini (Escola Superior de Agricultura 'Luiz de Queiroz'-USP-Departanento de Genetica Laboratorio de Genetica de Microraganismos) ;
  • Mortatti, Marcelo-Jose (Escola Superior de Agricultura 'Luiz de Queiroz'-USP-Departanento de Genetica Laboratorio de Genetica de Microraganismos) ;
  • Souza, Andre-Oliveira de (Universidade do Vale do Itajai, Centro de Ciencias Tecnologicas da Terra e do Mar) ;
  • Walter Maccheroni (Alellyx Applied Genomics) ;
  • Joao Lucio (Escola Superior de Agricultura 'Luiz de Queiroz'-USP-Departanento de Genetica Laboratorio de Genetica de Microraganismos, Universidade de Mogi das Cruzes Nucleo Integrado em Biotecnologia) ;
  • Welington Luiz (Escola Superior de Agricultura 'Luiz de Queiroz'-USP-Departanento de Genetica Laboratorio de Genetica de Microraganismos)
  • Published : 2004.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Enterobacter cloacae (strain PR2/7), a genetically modified endophyte(GME) in citrus plants, carrying different plasmids (pEC3.0/18, pCelE, pEglA and pGFP), was inoculated into Citrus sinensis seedlings under greenhouse conditions. The impact of this on the indigenous bacterial endophytic community was studied by analyses of 2 different morphologic groups. The germination rates of inoculated seeds were evaluated in greenhouse, and plasmid stability under in vitro conditions. Results demonstrated a great and diverse endophytic community inside plants, and specialization in tissue colonization by some bacterial groups, in different treatments. Shifts in seed germination rate were observed among treatments: in general, the PR2/7 harboring pEglA bacterial- clone significantly reduced seed germination, compared to the PR2/7 harboring pEC3.0/18 clone. This suggests that the presence of the pEglA plasmid changes bacteria-seed interactions. The endophytic community of citrus seedlings changed according to treatment. In seedlings treated with the PR2/7 with pEglA clone, the population of group II decreased significantly, within the context of the total endophytic community. These results indicate that the application of GMEs induces shifts in the endophytic bacterial community of citrus seedlings.

Keywords

References

  1. Araújo, W.L., W. Maccheroni Jr., C.I. Aguilar-Vildoso, P.A.V. Barroso, H.O. Saridakis, and J.L. Azevedo. 2001. Variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks. Can. J. Microbiol. 47, 229-236
  2. Araújo, W.L., J. Marcon, W. Maccheroni Jr., J.D. van Elsas, J.W.L. van Vuurde, and J.L. Azevedo. 2002. Diversity of endophytic bacterial population and interaction with Xylella fastidiosa in citrus plants. Appl. Environ. Microbiol. 68, 4906-4914
  3. Azevedo, J.L., W. Maccheroni Jr., J.O. Pereira, W.L. Araujo. 2000. Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Elect. J. Biotechnol [online]. April $15^{th}$. (http://www.ejbiotechnology.info/content/vol3/issue1/full/4/index.html). ISSN 0717-3458.
  4. Downing, K.J., G. Leslie, and J.A. Thomson. 2000. Biocontrol of the sugarcane borer Eldana saccharina by expression of the Bacillus thuringiensis cry1Ac7 and Serratia marcescens chiA genes in sugarcane-associated bacteria. Appl. Environ. Microbiol. 66, 2804-2810
  5. Fahey, J.W., M.B Dimock, S.F. Tomasino, J.M. Taylor, and P.S. Carlson. 1991. Genetically engineered endophytes as biocontrol agents: A case study in industry, p. 401-411. In: Microbial Ecology of Leaves. Springer Verlag (ed.), New York
  6. Gardner, J.M., J.A. Chandler, and A.W. Feldman. 1985. Growth response and vascular plugging of citrus inoculated with rhizobacteria and xylem-resident bacteria. Plant Soil. 86, 996-1000
  7. Gardner, J.M., A.W. Feldman, and M. Zablotowicz. 1982. Identity and behavior of xylem-residing bacteria in rough lemon roots of Florida citrus trees. Appl. Environ. Microbiol. 43, 1335-1342
  8. Glandorf, D.C.M., P. Verheggen, T. Jansen, J.W. Jorritsma, E. Smit, P. Leeflang, K. Wernars, L.S. Thomashow, E. Laureijs, J.E. Thomas-Oates, P.A.H.M. Bakker, and L.C. van Loon. 2001. Effect of genetically modified Pseudomonas putida WCS358r on the fungal rhizosphere microflora of field-grown wheat. Appl. Environ. Microbiol. 67, 3371-3378
  9. Hallmann, J., A. Quadt-Hallmann, W.F. Mahaffee, J.W. Kloepper. 1997. Bacterial endophytes in agricultural crops. Can. J. Microbiol. 43, 895-914
  10. Holt, J.G., N.R. Kreig, P.H.A. Sneath, J.T. Staley, and S.T. Williams. 1994. Bergeys Manual of Determinative Bacteriology. 9th ed. Williams & Wilkins, Baltimore, MD, USA. 787 pp
  11. Lampel, J.S., G.L. Canter, M.B. Dimock, J.L. Kelly, J.J. Anderson, B.B. Uratani, J.S. Foulke Jr., and J.T. Turner. 1994. Integrative cloning, expression, and stability of the cryIA(c) gene from Bacillus thuringiensis subsp. kurstati in a recombinant strain of Clavibacter xyli subsp. cynodontis. Appl. Environ. Microbiol. 60, 501-508
  12. Mocali, S., E. Bertelli, F. Di Ciello, A. Mengoni, A. Sfalanga, F. Viliani, A. Caciotti, S. Tegli, G. Surico, and R. Fani. 2003. Fluctuation of bacteria isolated from elm tissues during different seasons and from different plant organs. Res. Microbiol. 54, 105-114
  13. Peixoto-Neto, P.A.S., J.L. Azevedo, and W.L. Araújo. 2002. Microrganismos endofíticos: interação com plantas e potencial biotecnológico. Biotecnol. Ciência Desenvol. 29, 62-76
  14. Schmalenberger, A. and C.C. Tebbe. 2003. Genetic profiling of noncultivated bacteria from rhizosphere of sugar beet (Beta vulgaris) reveal field and annual variability but no effect of transgenic herbicide resistance. Can. J. Microbiol. 49, 1-8
  15. Sturz, A.V., B.R. Christie, and J. Nowak. 2000. Bacterial endophytes: Potential role in developing sustainable systems of crop production. Crit. Ver. Plant Sci. 19, 1-30