• Journal of the Korean Society for Marine Environment & Energy
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• v.7 no.3
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• pp.146-151
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• 2004

Cyclodextrin compounds including 2-hydroxypropyl-β-cyclodextrin(β-HPCD) though to be accelerate the biodegradation of PAHs molecule by increasing solubility of PAHs through detaining PAHs in their's cavity. However, only this mechanism is not sufficient to explain the enhancement of PAHs biodegradation by β-HPCD. To find out possible additional role of β-HPCD in the enhancement of PAHs biodegradation, biodegradation rates of pyrene and benzo[a]pyrene (B[a]P) by a PAHs degrading Novosphingobium pentaromtivorans US6-1 strain were compared between with and without addition of β-HPCD. Changes of bacterial biomass were also measured simultaneously. In addition catechol 1,2-dioxygenase activity was determined depending on pre-incubation conditions. As a result, β-HPCD accelerate the degradation rate of pyrene by strain US6-1 and especially the β-HPCD amendment was obligatory for the degradation of B[a]p. Bacterial biomass was responsible for β-HPCD, however, PAHs compounds such as pyrene and B[a]P did not contribute to the bacterial biomass. Catechol 1,2-dioxygenase specific activity of US6-l cells pre-cultured in MM2 medium containing l％ β-HPCD was higher than that of cells pre-cultured in ZoBell medium. The former case also showed similar activity compared to that of cells serially starved in MM2 medium after grown in ZoBell medium. These results imply that the presence of β-HPCD accelerate the degradation of PAHs by increasing the bacterial biomass as well as by increasing the water solubility of PAHs.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.5
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• pp.653-659
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• 2016

The purpose of the study is to investigate the correlation between the amount of antimicrobial agent (Defined Daily Dose, DDD) and antimicrobial resistance rate (%). The treatment of infectious diseases is becoming increasingly difficult, due to the increase in the number of multi-drug resistant bacteria, making it a clinically significant problem. Among the various factors, antimicrobial abuse is a major cause of antimicrobial resistance. The study was conducted on inpatients in a secondary university hospital in the central region utilizing the hospital's computerized statistical data and microbiological program of laboratory medicine from January 2010 to December 2014 pertaining to the dose of antimicrobial drugs for Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli strains isolated from blood culture. We analyzed the antimicrobial resistance rate per dose with the Pearson correlation coefficient. A significant (positive?) correlation was detected between the cefepime dose and the resistance of E. coli (P<0.033; r=0.907), while a significant negative correlation was found between the tobramycin dose and the resistance of E.coli. (P<0.028; r=-0.917). The aminoglycoside resistance of A. baumannii showed a significant negative correlation (P<0.048; r=-0.881), and the aminoglycoside resistance of E. coli showed a significant negative correlation as well (P<0.001; r=-0.992). In conclusion, the amount of antimicrobial agent (Defined Daily Dose, DDD) (is partly related to) the bacterial strain and its antimicrobial resistance rate (%).

• Clean Technology
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• v.29 no.2
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• pp.135-144
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• 2023

Pseudomonas aeruginosa and Staphylococcus aureus are the two most frequently encountered pathogens responsible for chronic wound infections, often coexisting in such cases. These infections exhibit heightened virulence compared to single infections, leading to unfavorable patient outcomes. The interaction among microorganisms within polymicrobial infections has been shown to exacerbate disease progression. Polymicrobial infections, prevalent in various contexts such as the respiratory tract, wounds, and diabetic foot, typically involve diverse microorganisms, with Pseudomonas aeruginosa and Staphylococcus aureus being the most commonly identified pathogens. This study aimed to compare the growth patterns of bacteria under a concentration gradient of toxic chemicals, focusing on a Gram-negative strain of Pseudomonas aeruginosa and a Gram-positive strain of Staphylococcus aureus. The minimum inhibitory concentration (MIC), which signifies the concentration at which bacterial growth is inhibited, was determined by performing broth microdilution and assessing the bacteria's growth curves. The growth curves of both Pseudomonas aeruginosa and Staphylococcus aureus were confirmed, and the exponential growth phases were applied to calculate the doubling times of bacteria. The MIC value for each toxic chemical was determined through broth microdilution. These results allowed for the identification of disparities in growth rates between Gram-positive and Gram-negative bacteria, as well as differences in resistance to individual toxic substances. We expect that this approach has a strong potential for further development towards the innovative treatment of bacteria-associated infections.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.4
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• pp.383-390
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• 2015

This study conducted tensile tests on SA508 Gr.1a low alloy steel and SA312 TP316 stainless steel piping materials under various strain rates at room temperature (RT) and $316^{\circ}C$ to investigate the effects of loading rate on the deformation behavior of nuclear piping materials. At RT, the deformation behavior for both pipe materials showed a typical loading rate dependence, i.e., the strength increased and the ductility decreased as the loading rate increased. At $316^{\circ}C$, however, the strength and elongation of SA508 Gr.1a low alloy steel decreased as the loading rate increased, and its reduction of area non-linearly varied with the loading rate. For SA312 TP316 stainless steel, the strength, elongation, and reduction of area at $316^{\circ}C$ were almost the same regardless of the loading rate. At both temperatures, the strain hardening capacity was nearly independent of the loading rate for SA508 Gr.1a low alloy steel, while it decreased with increasing loading rate for SA312 TP316 stainless steel.

• Microbiology and Biotechnology Letters
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• v.20 no.1
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• pp.79-84
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• 1992

The Photosynthetic bacterium, Rhodopseudomonas sphaeroides strain B6 was irnmobilized on agar gel. The optimum concentration of agar for hydrogen production was 2% (w/v). Maximum rates of hydrogen production by immobilized (300 ml of gel; 2.85 rng dry cells/ml) and free cells (1l culture; 0.87 mg dry cells/ml) were 47.5 and 48.0 ml/hr/culture, respectively. However, when both cultures were fed by 10 mmoles of lactate as limited electron donor at the later period of incubation, the activity of hydrogen production by free cells was significantly decreased but, immobilized cells continued hydrogen production with almost the same initial rate. Wc examined hydrogen production by immobilized cells of strain B6 under periodic illumination for 12 hr-intervals. When the culture was periodically fed by basal medium containing 9.3 rnmoles of DL-lactate and 1.86 mmoles of L-glutamate as consumed electron donor and nitrogen source, respectively, for every one liter of hydrogen produced, hydrogen was evolved continuously with the average rate of 510 ml/day/300 ml gel (2.9 rng dry cellslml) during the incubation time for 228 hr.

• The Korean Journal of Mycology
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• v.46 no.2
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• pp.177-185
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• 2018

The cultivation characteristics of 26 wild strains of Lentinula edodes were investigated for their use as breeding material. Strains NIFoS 68, 136, 1521, 1651, and 2064 showed an above average mycelial growth on potato dextrose agar at 10, 20, and $30^{\circ}C$. NIFoS 411 showed the lowest mycelial growth at $20^{\circ}C$, but the highest growth at $30^{\circ}C$. The rate of weight loss of L. edodes cultivated on sawdust (2 kg) ranged from 13.5 to 47.5%, with the highest rates showed by NIFoS 50 (47.5%), NIFoS 128 (34.5%), and NIFoS 54 (34.4%). Fruiting bodies were produced in nearly all (24/26) strains and productivity ranged from 3 g to 446 g/2 kg medium. Temperature was not significantly correlated with mushroom production or mycelial growth. Larger weight loss correlated strongly with fruit yield. In terms of production, NIFoS 50 (446 g), NIFoS 952 (435 g), and NIFoS 53 (421 g) were useful as breeding material. The NIFoS 667 strain was superior in terms of morphology. NIFoS 670 showed the characteristic yellowish-brown color of fruiting bodies.

• Journal of the Korea Concrete Institute
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• v.24 no.5
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• pp.605-612
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• 2012

Spalling of concrete occurs due to vapor pressure ignited explosion, temperature difference across a section, and combination of these factors. Factors affecting spalling can be classified into internal and external factors such as material property and environmental condition, respectively, have to be considered to precisely understand spalling behavior. An external environmental factor such as differences in heating rate cause internal humidity cohesion and different vapor pressure behavior. Therefore, spalling property, vapor pressure and thermal strain property were measured from concrete with compressive strengths of 30 MPa, 50 MPa, 70 MPa, 90 MPa, and 110 MPa, applied with ISO-834 standard heating curve of $1^{\circ}C/min$ heating rate. The experimental results showed that spalling occurred when rapid heating condition was applied. Also, when concrete strength was higher, the more cross section loss from spalling occurred. Also, spalling property is influenced by first pressure cancellation effect of thermal expansion caused by vapor pressure and heating rates.

• Microbiology and Biotechnology Letters
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• v.17 no.2
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• pp.100-108
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• 1989

Thirty-six aromatic compound biodegraders; 10 strains for benzoate, 10 for salicylate, 6 for m-toluate, and 10 for DL-camphor were isolated and taxonomically characterized. A mutant Pseudomonas strain, Ben 6-2, derived from Ben 6 revealed remarkably improved ability to metabolize benzoate. Thus enhancement of the average substrate removal rate from 5.2 to 11.0mg/$\ell$/ hr was attained by the mutant. Both of strains Sal 7 and Tol 2, degraders of salicylate and m-toluate respectively, were classified as Pseudomonas sup. Both strains were found to be extremely effective in metabolizing each aromatic substrates. The average substrate degradation rates in minimal salt media containing 2,200mg/$\ell$ of the substrate were calculated to be 40.1 mg/$\ell$/ hr for strain Sal 7 and 33.0mg/$\ell$/ hr for Tol 2. Cam 10, a camphor degrading strain was demonstrated to be capable of mineralizing benzoate, phenol, toluene, octane, cyclohexane and xylene as well as camphor. Strain 1040 isolated from Cam 10 after repented adaptation to 1,000 mg/$\ell$ m-toluate gained the ability to utilize toluate as a sole carbon source. The mutant Brew actively at the expense of a mixture of car-bon sources; camphor, m-toluate, benzoate and phenol (each: 200 mg/$\ell$) and utilized the substances in the preferential order of camphor, phenol, benzoate, and m-toluate. Among the biodegraders examined Cam 1040 and Tol 2 were detected to harbor plasmid. The plasmid from Cam 1001 was determined to be about 98kb, and evidenced to encode the enzyme(s) for the degradation of camphor. For the further diversification of the metabolic potentials of Cam 1040, the NAH 2 plasmid of Pseudomonas putida NCIB 9816 was transferred to Cam 1040 by conjugation. The exconjugant obtained, Cam 1043, proved to gain an additional ability to metabolize salicylate and naphthalene.

• Journal of Aquaculture
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• v.20 no.2
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• pp.85-89
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• 2007

This study examined the hatching rate of eyed-eggs and growth of fingerlings of USA strain rainbow trout, Oncorhynchus mykiss, which were transplanted on Nov. 17, 2005 from the USA for improvement of breeding. The diameter and weight of eyed-eggs were $4.95{\pm}0.34\;mm$ and $0.07{\pm}0.003\;g$, respectively. The eyed-eggs were hatched in 8 days later and hatching rates were $77.3{\sim}98.6%$. After 56 days from hatching, the fingerlings grew to $4.0{\pm}0.7$ cm and 0.62 g in total length and body weight, respectively. The relationships between total length (TL) and elapsed days (ED) were expressed as TL=0.0366ED+1.8645 ($r^2=0.928$, n=350). The relationships between body weight (BW) and elapsed days (ED) were expressed as following; $BW=0.0638e^{0.0408ED}\;(r^2=0.9917,\;n=350)$. And survival of rainbow trout was $64.5{\pm}1.3%$ in 56 days after hatching.

• Applied Biological Chemistry
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• v.40 no.6
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• pp.484-489
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• 1997

The characteristics of growth and esterase activity of bacterial strains isolated from acid hydrolysed soybean protein were examined. All the isolated strains having decomposition activity of p-hydroxybenzoic acid butyl ester and esterase producing activity were identified as Bacillus sp. by morphological and biochemical methods. The specific growth rates, esterase activities and p-hydroxybenzoic acid butyl ester decomposition activities of isolated strains were $0.844{\sim}1.213\;h^{-1}$, $21{\sim}222\;mU/ml$ and $5.4{\sim}8.1\;mU/ml$, respectively. In the fermentation of Bacillus sp. KB8 strain which had the highest esterase producing activity, growth, extracellular excretion and intracellular synthesis of esterase were inhibited by adding NaCl in the culture broth. Esterase producing activity gradually increased after late exponential growth phase, until maximum value of 420 mU/ml reached after 64 hours culture period. Esterase of Bacillus sp. KB8 strain was stable up to $50^{\circ}C$ for 30 minutes, but was inactivated by heating for 30 minutes at $70^{\circ}C$. The enzyme activity exponentially decreased during the incubation time at the temperatures of $60^{\circ}C$ and $65^{\circ}C$.