• Journal of Microbiology and Biotechnology
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• v.8 no.5
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• pp.461-465
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• 1998

The suspension culture of an anchorage-dependent cell line (Bok-l) from Bombina orientalis was successful in respects of cost and efficiency. The amount of cells obtained from the suspension culture was almost equivalent to that from the anchorage-dependent culture. This result shows the possibility of suspension culture for scale-up. The cells in suspension produced an antibacterial peptide as much as anchorage-dependent cells did. The cell growth ($6.0\times10^6cells/m\ell$) and viability (>80%) at 10 rpm were higher than that at 0 rpm ($1.9\times10^6cells/m\ell$, 65~80%) and 30 rpm ($1.8\times10^6cells/m\ell$ 40~76%). The size of cells became smaller at the agitation rate of 30 rpm. The antibacterial activities of cell extracts from suspension cultured cells were confirmed against gram-negative and gram-positive bacteria by the inhibition zone assay and the liquid growth inhibition assay.

• Journal of The Korean Astronomical Society
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• v.15 no.2
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• pp.71-77
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• 1982

According to the star formation rate and metal enrichment rate given by the disk-halo model of Lee and Ann (1981), the two different forms of time-dependent initial mass function (IMF) and the present day mass function (PDMF) of nearby stars have been examined. It was shown that the constraint for the initial rapid metal enrichment requires the time-dependence of IMF at the very early phase ($t{\lesssim}5{\times}10^8$ yrs) of the solar neighborhood. The computed PDMF's show that the PDMF is nearly independent of any specific functional form of IMF as long as the latter includes a Gaussian distribution of log m. This result is due to the very small fractional mass $({\times}5%)$ of stars formed at the very early period during which the IMF is time-dependent. The computed PDMF suggests the presence of more numerous low mass stars than shown in Miller and Scalo's (1979) PDMF, supporting the possibility of the existence of low-velocity M dwarfs. According to the number distribution of stars with respect to [Fe/H], the mean age of these low mass star must be very old so as to yield the mean metal abundance $\bar{[Fe/H]}{\approx}-0.15$ for the stars in the solar neighborhood.

• Journal of the Korean Institute of Telematics and Electronics T
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• v.36T no.2
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• pp.56-62
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• 1999

This paper described a voice dialing system(VDS) and its hardware implementation for a speaker-dependent recognition of Korean digit speech using duty cycle. The proposed VDS consist of integrator, leveling divider circuit and recognition program. The analog speech signal is applied to the VDS through the low-pass filter cutoff frequency is 4.5(kHz). It is thoroughly confirmed that the speaker-dependent recognition of Korean digit speech is well behaved by the hardware system. Experimental results show that the recognition rate is 64% in average for Korean digit speech. Moreover, a high recognition rate of 100% is obtained for digits; /4/, /5/, /6/, /7/, /9/, /0/.

• Smart Structures and Systems
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• v.4 no.6
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• pp.809-828
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• 2008

Real-time hybrid testing is an attractive method to evaluate the response of structures under earthquake loads. The method is a variation of the pseudodynamic testing technique in which the experiment is executed in real time, thus allowing investigation of structural systems with time-dependent components. Real-time hybrid testing is challenging because it requires performance of all calculations, application of displacements, and acquisition of measured forces, within a very small increment of time. Furthermore, unless appropriate compensation for time delays and actuator time lag is implemented, stability problems are likely to occur during the experiment. This paper presents an approach for real-time hybrid testing in which time delay/lag compensation is implemented using model-based response prediction. The efficacy of the proposed strategy is verified by conducting substructure real-time hybrid testing of a steel frame under earthquake loads. For the initial set of experiments, a specimen with linear-elastic behavior is used. Experimental results agree well with the analytical solution and show that the proposed approach and testing system are capable of achieving a time-scale expansion factor of one (i.e., real time). Additionally, the proposed method allows accurate testing of structures with larger frequencies than when using conventional time delay compensation methods, thus extending the capabilities of the real-time hybrid testing technique. The method is then used to test a structure with a rate-dependent energy dissipation device, a magnetorheological damper. Results show good agreement with the predicted responses, demonstrating the effectiveness of the method to test rate-dependent components.

• Transactions of Materials Processing
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• v.20 no.3
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• pp.229-235
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• 2011

The formability of magnesium alloy sheets at room temperature is generally low because of the inherently limited number of slip systems, but higher at temperatures over $150^{\circ}C$. Therefore, prior to the practical application of these materials, the forming limits should be evaluated as a function of the temperature and strain rate. This can be achieved experimentally by performing a series of tests or analytically by deriving the corresponding modeling approaches. However, before the formability analysis can be conducted, a model of flow stress, which includes the effects of strain, strain rate and temperature, should be carefully identified. In this paper, such procedure is carried out for Mg alloy AZ31 and the concept of flow stress surface is proposed. Experimental flow stresses at four temperature levels ($150^{\circ}C$, $200^{\circ}C$, $250^{\circ}C$, $300^{\circ}C$) each with the pre-assigned strain rate levels of $0.01s^{-1}$, $0.1s^{-1}$ and $1.0s^{-1}$ are collected in order to establish the relationships between these variables. The temperature-compensated strain rate parameter which combines, in a single variable, the effects of temperature and strain rate, is introduced to capture these relationships in a compact manner. This study shows that the proposed concept of flow stress surface is practically relevant for the evaluation of temperature and strain dependent formability.

• Reproductive and Developmental Biology
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• v.35 no.4
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• pp.475-478
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• 2011

In the present study, the effect of cysteine and NT or bisphenol A(BP) on in vitro aturation(IVM) of porcine oocytes were examined. COCs was cultured in NCSU-23 medium supplement with 10% FCS which had previously been covered with mineral oil and equilibrated in a humidified atmosphere of 5% $CO_2$ and 95% air at $38^{\circ}C$. The IVM rate of oocytes cultured for 48 hrs in NCSU-23 medium supplement with 0.5~10.0 mM cysteine were $34.0{\pm}3.2%$, $36.0{\pm}3.5%$, $48.0{\pm}3.8%$, $22.0{\pm}3.2%$, respectively. The IVM rate of oocytes cultured in NCSU-23 medium supplement with 0.5~5.0mM NT for 48 hrs were $24.0{\pm}4.2%$, $18.0{\pm}4.9%$, $8.0{\pm}2.2%$, respectively. NT affects oocyte in vitro maturation rate in a dose-dependent. This result were significantly lower than the control group. The IVM rate of oocytes cultured for 48 hrs in NCSU-23 medium supplement with 1.0 mM NT+5.0 mM cysteine($38.0{\pm}4.3%$) were significantly higher than that of NT treatment. The IVM rate of oocytes cultured in NCSU-23 medium supplement with 0.05~5.0 mM BP for 48 hrs were $20.0{\pm}4.7%$, $10.0{\pm}5.3%$, $6.0{\pm}3.2%$, respectively. The IVM rate of oocytes cultured in NCSU-23 medium supplement with BP was significantly lower cultured non supplement of BP ($44.0{\pm}3.5%$). BP affects porcine oocyte maturation rate in a dose-dependent manner. The IVM rate of oocytes cultured for 48 hrs in NCSU-23 medium supplement with 1.0 mM BP+5.0 mM cycteine ($32.0{\pm}3.2%$) were increased than that of BP treatment.

• Computers and Concrete
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• v.9 no.4
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• pp.311-325
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• 2012

In this paper, we examine the behavior of the High-Rate Brittle Microplane (HRBM) concrete model based on a series of penetration experiments. These experiments were conducted with three different slab thicknesses (127, 216 and 254 mm) that provided a significant challenge for the numerical simulations. The 127 mm slab provided little resistance, the 216 mm slab provided nominal resistance and the 254 mm slab approached the perforation limit thickness of the projectile. These experiments provide a good baseline for evaluating material models since they have been shown to be extremely challenging; in fact, we have not encountered many material models that can provide quantitatively predictive results in terms of both projectile exit velocity and material damage. In a companion paper, we described the HRBM material model and its fit to various quasi-static material property data for WES-5000 concrete. In this paper, we show that, when adequately fit to these quasi-static data, the HRBM model does not have significant predictive capabilities, even though the quasi-static material fit may be exceptional. This was attributed to the rate-dependent response of the material. After various rate effects were introduced into the HRBM model, the quantitative predictive nature of the calculations dramatically increased. Unfortunately, not much rate-dependent material property data are in the literature; hence, accurate incorporation of rate effects into material models is difficult. Nonetheless, it seems that rate effects may be critical in obtaining an accurate response for concrete during projectile perforation events.

• Structural Engineering and Mechanics
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• v.22 no.3
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• pp.279-292
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• 2006

A new approach using the differential quadrature method (DQM) is derived for analysis of non-uniform beams resting on nonlinear media in this study. The influence of velocity dependent viscous damping and strain rate dependent viscous damping is investigated. The results solved using the DQM have excellent agreement with the results solved using the FEM. Numerical results indicated that the DQM is valid and efficient for non-uniform beams resting on non-linear media.

• Journal of Korean Society for Quality Management
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• v.20 no.1
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• pp.11-21
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• 1992

The Purpose of this paper contribute to design the multistate Markov process for the reliability of a system when the transition-rates of each unit depend on the current state of the system. The system transition-rate matrix has the form of the kronecker sum of transition rate matrices for the units, is analyzed and investigated. As a result, the system which has the state-dependent units is detaily analyzed and introduced by the approach of an algorithm for the system transition-rate matrix based on the kronecker algebra.

• Journal of the Korean Data and Information Science Society
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• v.23 no.6
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• pp.1289-1297
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• 2012

We consider a general compound Poisson risk model in which the premium rate is surplus dependent. We analyze the joint distribution of the surplus immediately before ruin, the deffcit at ruin and the time of ruin by solving the integro-differential equation for the Gerber-Shiu discounted penalty function.