• Biomolecules & Therapeutics
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• 제10권4호
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• pp.253-257
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• 2002

This study was performed to investigate therapeutic effects of Achyranthis Radix extract and chitosan on the growth and differentiation of rat calvarial cells. it was found that treatment of methanol extract of Achyranthis Radix for 2 days caused 2.4-fold increase in the growth of rat calvarial cells. However, chitosan treatment caused only 1.9-fold increase in the cell growth. Treatment of methanol extract of Achyranthis Radix for 14 days caused 2-fold increase in the growth of rat calvarial cells. Alkaline phosphatase activity, one of the markers for bone cell differentiation, was increased approximately by 1.7-fold and 2.9-fold by the treatment of methanol extract of Achyranthis Radix for 2 days and 14 days, respectively. These results suggest that Achyranthis Radix extract could be beneficial for bone regeneration.

• Journal of Magnetics
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• 제21권4호
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• pp.599-602
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• 2016

Fluoride (F) is an important element for the mineralization of body tissues. The purpose of this study was to administer fluoride prenatally to rats to evaluate its beneficial concentration for rat bone using microstructural analysis, to analyze its effect on the bone structure, and to evaluate the effect of its transfer through rat placenta. Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectrometry (NMR) were performed. The $^{19}F$ NMR and $^{31}P$ NMR signals suggested the existence of fluoride ions in the apatite lattice because the signals were caused by the fluoride ions that were coupled to the phosphate atoms and were affected in the phosphate phases other than the element phases in the apatite. Consequently, if it was not affected too much, the desirable concentration of prenatal fluoride treatment could have a helpful effect on the bone crystal structure through placental fluoride transfer.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제37권
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• pp.16.1-16.7
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• 2015

Background: This study aimed to investigate new bone formation using recombinant human bone morphogenetic protein 2 (rhBMP-2) and locally applied bisphosphonate in rat calvarial defects. Methods: Thirty-six rats were studied. Two circular 5 mm diameter bony defect were formed in the calvaria using a trephine bur. The bony defect were grafted with $Bio-Oss^{(R)}$ only (group 1, n = 9), $Bio-Oss^{(R)}$ wetted with rhBMP-2 (group 2, n = 9), $Bio-Oss^{(R)}$ wetted with rhBMP-2 and 1 mM alendronate (group 3, n = 9) and $Bio-Oss^{(R)}$ wetted with rhBMP-2 and 10 mM alendronate (group 4, n = 9). In each group, three animals were euthanized at 2, 4 and 8 weeks after surgery, respectively. The specimens were then analyzed by histology, histomorphometry and immunohistochemistry analysis. Results: There were significant decrease of bone formation area (p < 0.05) between group 4 and group 2, 3. Group 3 showed increase of new bone formation compared to group 2. In immunohistochemistry, collagen type I and osteoprotegerin (OPG) didn't show any difference. However, receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL) decreased with time dependent except group 4. Conclusion: Low concentration bisphosphonate and rhBMP-2 have synergic effect on bone regeneration and this is result from the decreased activity of RANKL of osteoblast.

• Journal of Periodontal and Implant Science
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• 제28권1호
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• pp.87-102
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• 1998

The purpose of this stuffy was to assess and compare the osseous responses to implanted particles of porous synthetic HA (Interpore $200^{(R)}$, Interpore International, U.S.A.), resorbable natural bovine derived HA (Bio-$oss^{(R)}$, Gestlich Pharma, Switzerland) and calcium carbonate(Biocoral $450^{(R)}$, Inoteb, France) in bone defects. Four calvarial defects of 2.5mm diameter were created in earth of 16 Sprague-Dawley rats. The experimental materials were subsequently implanted hi three defects, leaving the fourth defect for control purpose. Four animals were earth sacrificed at 3 days, 1week, 2weeks and 4 weeks after surgery. The tissue response was evaluated under light microscope. Overall, histologic responses showed that all the particles were well tolerated and caused no aberrent tissue responses. There were difference in the amount of newly formed bone at the experimental sites and control site. There was more new bone formation associated with calcium carbonate site. In addition, the calcium carbonate site displayed multinucleated giant cells surrounding calcium carbonate particles after the 1st week, and osteoid tissue within the particle after the 2nd week. After 4 weeks, calcium carbonate particles were resorbed and replaced with new bone. The healing of the natural bovine derived HA site was similar to that of porous synthetic HA, except that new bone growth between the two particles have progressed more in the former site after the 2nd week. In the natural bovine derived HA site, the particle was surrounded by newly formed bone after the 4th week. After 4 weeks, the control site showed more mature bone than other sites. In conclusion, the grafted site were better in new bone formation than non-grafted sites. In particular the calcium Carbonate site showed the ability of osteoinduction and natural bovine denver HA showed osteoconduction in rat calvarial defects. This suggest that calcium carbonate and natural bovine derived HA could enhance the regenerative potential in periodontal defects.

• 한국임상수의학회지
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• 제27권4호
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• pp.325-329
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• 2010

본 연구에서는 hydroxyapatite/poly $\varepsilon$-caprolactone composite (HA/PCL) 지지체와 matrigel을 랫드의 두개골 결손부 모델에 함께 이식 시의 골형성 정도를 평가하였다. 두개골결손부는 Sprague Dawley rat (n = 18)에서 수술적으로 형성하였으며 실험군은 Matrigel과 함께 HA/PCL 지지체를 이식한 군(M-HA/PCL group, n = 6)과 HA/PCL 지지체단독이식군(HA/PCL group, n = 6)으로 나누었고 대조군(CD group, n = 6)에는 아무 것도 이식하지 않았다. 수술 4주 후, 골형성은 방사선촬영, micro CT 및 조직검사를 통해 평가되었다. 방사선상에서 CD군의 골형성은 관찰되지 않았으나 HA/PCL과 M-HA/PCL군에서는 관찰되었고 골과 유사한 방사선비투과성이 M-HA/PCL군에서 더 많이 관찰되었다. Micro CT 평가에서 골부피는 HA/PCL군보다 M-HA/PCL군에서 더 높았으나 두 군 사이의 유의적 차이는 관찰할 수 없었다. 그러나 골밀도에서는 HA/PCL군보다 M-HA/PCL군이 더 유의적으로 높음을 확인할 수 있었다(p < 0.05). 조직학적 검사에서는 CD군에서 새로운 골은 원래 존재하던 골로부터만 형성되었으며 두개골결손부 내의 골형성은 보이지 않았다. HA/PCL군에서 새로운 골형성은 원래 존재하던 골로부터만 유래되었으나 M-HA/PCL군은 가장 많은 골형성을 보여주었으며 새로운 골이 원래 존재하던 골과 HA/PCL지지체 주변에서도 관찰되었다. 이러한 결과로 미루어볼 때 HA/PCL 지지체와 matrigel을 함께 사용하는 것이 골의 임계결손부에서 골형성을 증대시키는 효과적인 방법이 될 수 있을 것으로 생각된다.

• 대한임상검사과학회지
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• 제37권1호
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• pp.35-40
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• 2005

This paper reports the morphological nature of the remodelled interface process between implants and surrounding bone after 1, 4, 6, 8 and 12 weeks of implantation of smooth machined implants into rat tibias. After 4 weeks of implantation, histochemical analysis showed that the new bone was growing in direct contact with the implant. In the forming process, the activatived osteoblast cells migrated toward the interface and colonized the surface at the contact areas. This immature woven bone, rich in osteocyte lacunae, was deposited directly onto the implant surface. Osteoblast activity was found to continue ill 12 weeks of implantation The osteoblasts in lacunar areas developed numerous processes and synthesized bone matrix, after all, surrounded by secreting matrix. At the 12th week, the amount of newly formed bone matrix between bone and implant increased in mineralization. The mineralized mature bone contained well organized collagen fibers with characteristic banding pattern bone tissue formation around the implant.

• 한국가축번식학회지
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• 제24권2호
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• pp.189-197
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• 2000

본 연구는 골다공증유발 rat에 녹용추출물의 투여가 예방 및 치료효과에 미치는 영향을 구명하고자, 양측 난소를 적출한 후 골다공증이 유발된 rat에 암, 수녹용 추출물 525, 1,250 mg/kg을 투여했을 때 체중, 대퇴골의 중량, 장기중량, 골회분량의 측정 및 조직학적, 전자현미경적 검사 등을 실시하였다. 1. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 체중은 정상대조군에 비하여 유의한 증가를 나타냈다 (p＜0.05). 2. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 우측, 좌측의 femur 중량은 정상대조군에 비하여 감소하였다. 3. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 골회분량은 정상대조군에 비하여 약간 감소된 치를 나타냈으나 유의성은 인정되지 않았다. 4. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 간과 비장 및 신장의 각각 정상대조군에 비하여 감소된 치를 나타냈다. 5. 골다공증유발 rat에 암, 수녹용 추출물을 투여했을 때 조직학적 및 전자현미경적 소견은 정상대조군에서는 소주골간의 연결이 비교적 잘 유지되어 있고 골소실을 발견할 수 없었으나 난소적출군은 정상군에 비하여 소주골이 가늘어졌거나 소주골간 연결부분의 대다수가 끊어져 골양이 많이 소실되었음이 관찰되었으며, 녹용처리군에서는 소주골의 형태가 굵고 연결부분이 잘 유지되어 정상군과 유사한 소견이 관찰되었다. 위의 결과들에서 암, 수녹용 추출액은 난소제거후의 골소설을 억제하므로 골다공증의 예방과 치료에 효과가 있는 것으로 판단된다.

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 2004년도 제44회 종합학술대회 연제초록
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• pp.157-158
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• 2004

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 추계학술발표회
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• pp.258-259
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• 2011

• Maxillofacial Plastic and Reconstructive Surgery
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• 제38권
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• pp.14.1-14.6
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• 2016

Background: In guided bone regeneration (GBR) technique, many materials have been used for improving biological effectiveness by adding on membranes. The new membrane which was constructed with chitin-fibroin-hydroxyapatite (CNF/HAP) was compared with a collagen membrane (Bio-$Gide^{(R)}$) by means of micro-computed tomography. Methods: Fifty-four rats were used in this study. A critical-sized (8 mm) bony defect was created in the calvaria with a trephine bur. The CNF/HAP membrane was prepared by thermally induced phase separation. In the experimental group (n = 18), the CNF/HAP membrane was used to cover the bony defect, and in the control group (n = 18), a resorbable collagen membrane (Bio-$Gide^{(R)}$) was used. In the negative control group (n = 18), no membrane was used. In each group, six animals were euthanized at 2, 4, and 8 weeks after surgery. The specimens were analyzed using micro-CT. Results: Bone volume (BV) and bone mineral density (BMD) of the new bone showed significant difference between the negative control group and membrane groups (P < 0.05). However, between two membranes, the difference was not significant. Conclusions: The CNF/HAP membrane has significant effect on the new bone formation and has the potential to be applied for guided bone regeneration.