• Molecules and Cells
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• 제37권5호
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• pp.372-382
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• 2014

In this study, the chloroplast (cp) genome sequences from three early diverged leptosporangiate ferns were completed and analyzed in order to understand the evolution of the genome of the fern lineages. The complete cp genome sequence of Osmunda cinnamomea (Osmundales) was 142,812 base pairs (bp). The cp genome structure was similar to that of eusporangiate ferns. The gene/intron losses that frequently occurred in the cp genome of leptosporangiate ferns were not found in the cp genome of O. cinnamomea. In addition, putative RNA editing sites in the cp genome were rare in O. cinnamomea, even though the sites were frequently predicted to be present in leptosporangiate ferns. The complete cp genome sequence of Diplopterygium glaucum (Gleicheniales) was 151,007 bp and has a 9.7 kb inversion between the trnL-CAA and trnV-GCA genes when compared to O. cinnamomea. Several repeated sequences were detected around the inversion break points. The complete cp genome sequence of Lygodium japonicum (Schizaeales) was 157,142 bp and a deletion of the rpoC1 intron was detected. This intron loss was shared by all of the studied species of the genus Lygodium. The GC contents and the effective numbers of codons (ENCs) in ferns varied significantly when compared to seed plants. The ENC values of the early diverged leptosporangiate ferns showed intermediate levels between eusporangiate and core leptosporangiate ferns. However, our phylogenetic tree based on all of the cp gene sequences clearly indicated that the cp genome similarity between O. cinnamomea (Osmundales) and eusporangiate ferns are symplesiomorphies, rather than synapomorphies. Therefore, our data is in agreement with the view that Osmundales is a distinct early diverged lineage in the leptosporangiate ferns.

• 대한지구과학교육학회지
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• 제8권2호
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• pp.128-138
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• 2015

The purpose of this study is to examine potential as a geological field course of Odongdo located to the east of Yeosu city, Korea. The total area and the height from the sea level of Odongdo is about 0.13km2 and 42m, respectively. Odongdo area belongs to the Hallyeohaesang National Marine Park, and is famous for rock scenery in the rocky coast and living of rare plants. For this study, diversity of geomorphology and geology was investigated at the sites called as dragon cave, windy area, seal rocks, sunrise viewing platform, second seashore rocks and first seashore rocks along the coast of Odongdo. The results of this study are as follows: A lot of geomorphological and geological resources like sea cliff, sea cave, strata, various sedimentary rocks(conglomerate, sandstone, mudstone), andesite, fault and examples of rock weathering were found in the rocky coast of the study area. These field resources are pertinent to the field work materials for the teaching and learning of geology units in elementary school science. Odongdo is to be recommended as a site of outdoor geological field course for the elementary school students inhabited in Yeosu city and neighborhood area because each site of the rocky coast in this study area has various field work materials and the wide space for observe activities, and is equipped with the facilities as a trail for rock observation.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 심포지엄
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• pp.9-18
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• 2003

A method for rapid propagation of mature Jack fruit was developed. Four types of explants (mature embryos, apical meristems of young seedlings, apices from mature plants and nodal segments) were used. It has been found 88% of young apical meristems produced shoots in Campbell and Durzan (CD) medium compared to 60% in Murashige and Skoog (MS) medium. Only 1/3 of them produced multiple shoots. Shoot idtiation from nodal segments was very rare. Mature apices produced callus. Although removed of the sheathing cover around mature buds enhanced the shoot initiation but success rate was low in growth regulator free medium. Embryos respond to the CD medium but not to the MS medium. Embryos from seeds soaked in water for 24 hours produced shoots after 8 weeks of incubation and the success rate was 70% while embryos from dry seeds only produced roots. There was no significant effect of cold storage (refrigeration) for 7 days on shoot initiation from mature embryos (65%) but the ability for shoot induction declines with storage time (55% after 21 days of cold storage). Mature axillary buds were established in Modified Campbell and Durzan (CD) medium supplemented with 0.5mg/1 and IBA. There was a significant difference in the growth performance of shoots according to the period of the year in which explants were collected. Highest (60%) was observed in November-January period. It was only 30% when the explants were collected in February-April or May-July and decreased to 20% in August-October. The shoots produced in November-January showed a higher vigor than those produced in other months. Since Jak fruit show seasonal changes in fruit bearing and shedding of leaves, it can be suggested that the difference in growth performances of tissues cultured in artificial culture media would have been affected by endogenous rhythms.

• Mycobiology
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• 제49권3호
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• pp.223-234
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• 2021

Itajahya rosea was found growing in association with Leucaena leucocephala plants at Savitribai Phule Pune University campus in India. The species identity was confirmed by phylogenetic analysis based on ITS and LSU regions of rDNA, wherein, our fugus was placed along with I. rosea in phylogenetic tree. It represents first record of I. rosea from India. Frequent visitation by Drosophila species on I. rosea fruiting body particularly on gleba was observed. The Drosophila got attracted to the detached gleba under the laboratory conditions and even sometimes, they prefer to sit over the gleba as compare to their food banana. It suggested that I. rosea gleba or pseudostipe produces some compounds for attraction and feeding behavior of Drosophila species. Therefore, we characterized the volatile attractants produced by gleba and pseudostipe of I. rosea by GC-MS analysis. Nineteen compounds were identified from gleba while nine compounds were recovered from the pseudostipe. Out of them, blends of three abundant odor producing volatile compounds were reported namely, Hexadecane, Pentadecane and Nonadecane, which are responsible for attraction of Drosophila toward the gleba. Three fatty acids namely 9,12-octadecadienoic acid (Z,Z), hexadecanoic acid and benzoic acid ethyl ester produced are served as an appetitive signal through olfactory response of Drosophila, so the flies were feed on the gleba. Two pheromones' compounds, heneicosane and (+)-(5S,9S)-5,9-dimethylpentadecane, were also reported in pseudostipe and gleba, respectively, which play a role in Drosophila for breeding. Our study highlights an intriguing chemical ecology of fungus-Drosophila interaction.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.90-90
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• 2019

This study was carried out to investigate the vegetation and vegetation structure of Mt. Man in Hwacheon Gun from April to October 2018. The survey site is located in the northern part of South Korea where the climate is similar to that of North Korea. The purpose of this study is to provide basic information for efficient ecological forest management after unification. A total of 341 vascular plant taxa are recorded, representing 221 genera, 4 subspecies, 42 varieties and 6 forms in 84 families. Rare species, including Crataegus komarovii and Amitostigma gracile are found across 10 taxa; 8 families, 10 genera, 9 species, 1 variety. Naturalized Plants, including Ambrosia artemisiifolia and Erigeron annuus found across 11 taxa; 6 families, 10 genera, 11 species. Dominant tree species of the tree layer based on the highest importance values were: Quercus mongolica (23.47%), Larix kaempferi (11.43%), Fraxinus rhynchophylla (8.30%), in the subtree layer were: Lindera obtusiloba (11.38%), Acer pictum subsp. mono (11.33%), Fraxinus rhynchophylla (9.80%) and in the shrub layer were: Lindera obtusiloba (10.22%), Fraxinus rhynchophylla (8.43%), Rubus crataegifolius (8.00%), in the herb layer Pteridium aquilinum var. latiusculum (5.67%), Oplismenus undulatifolius (5.00%), Lysimachia clethroides (3.49%) respectively. The species diversity of Mt. Man was 1.1764 in the tree layer, 1.2403 in the subtree layer, 1.4135 in the shrub layer, and 1.7416 in the herb layer. In conclusion, if we utilize the flora and vegetation structure surveyed in Mt. Man, we can effectively manage the degraded forests with similar altitudes and climate zones in North Korea.

• 식물분류학회지
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• 제52권2호
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• pp.102-107
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• 2022

Rhododendron keiskei var. hypoglaucum (Ericaceae) was recently reported in Korea, with a disjunct distribution on the southern islands of the Korean Peninsula. Although chromosome numbers and ploidy variations are important traits in angiosperms, gaining a clear understanding the cytological features of Rhododendron has been hampered by the small size of its chromosomes. We herein report the chromosome number, karyotype structure, and genome size of R. keiskei var. hypoglaucum for the first time. The chromosome number of the investigated plants was 2n = 26 with x = 13 as the base chromosome number, which is the one of the frequently encountered base chromosome numbers in Rhododendron. The karyotype of R. keiskei var. hypoglaucum is composed of metacentric and submetacentric chromosomes similar in length, which ranged from 1.39 to 2.40 ㎛. The DNA 1C-value in all examined accessions was small, ranging from 0.63 to 0.65 pg, further supporting the stable genome size in Rhododendron. These comprehensive cytological results provide a framework for detailed molecular, cytogenetic, and phylogenomic analyses that can be used to interpret the slow species diversification rate in Rhododendron.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제3권3호
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• pp.139-148
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• 2022

Saxifraga hirculus is classified as a vulnerable plant species in Europe, and it is confined to base-rich flushes in the British uplands. However, a lack of available information about its conservation status hampers the development of adaptive strategies for its in-situ conservation, especially with respect to grazing. To assess the effectiveness of sheep grazing in maintaining viable populations of S. hirculus, we compared the community dynamics of the vegetation in a base-rich flush over 44 years in two plots: one sheep-grazed under business-as-usual sheep grazing densities and the other fenced to exclude grazing. The plots were established in 1972, and the abundances of all vascular plants, bryophytes, and litter were measured at six intervals until 2016. Our results showed that although the presence of S. hirculus was maintained in both plots over the 44 years, it declined and reached a minimum between 1995 and 2010, when it was close to extinction. Since 2013, Saxifraga has recovered only slightly. Interestingly, the S. hirculus response appeared to be independent of grazing treatment, but it mirrored wider changes in the vegetation composition and structure within the flush over the 44 years. These changes are similar to others reported in broader uplands that have been attributed to a combination of reduced nitrogen and sulfur deposition and global warming. Thus, the simple adaptive management approach of "just managing" sheep grazing appeared ineffectual for preserving the S. hirculus population. S. hirculus showed signs of recovery at the end of the study period within this base-rich flush.

• Advances in nano research
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• 제13권4호
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• pp.325-339
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• 2022

Endophytes ascertain a symbiotic relationship with plants as promoters of growth, defense mechanism etc. This study is a first report to screen the endophytic population in Waltheria indica, a tropical medicinal plant. 5 bacterial and 3 fungal strains in leaves, 3 bacterial and 1 yeast species in stems were differentiated morphologically and identified by biochemical and molecular methods. The phylogenetic tree of the isolated endophytes was constructed using MEGA X. Silver nanoparticles were biosynthesized from a rare endophytic bacterium Cupriavidus metallidurans isolated from the leaf of W. indica. The formation of silver nanoparticles was confirmed by UV-Visible spectrophotometer that evidenced a strong absorption band at 408.5 nm of UV-Visible range with crystalline nature and average particle size of 16.4 nm by Particle size analyzer. The Fourier Transform Infra-Red spectrum displayed the presence of various functional groups that stabilized the nanoparticles. X-ray diffraction peaks were conferred to face centered cubic structure. Transmission Electron Microscope and Scanning Electron Microscope revealed the spherical-shaped, polycrystalline nature with the presence of elemental silver analyzed by Energy Dispersive of X-Ray spectrum. Selected area electron diffraction also confirmed the orientation of AgNPs at 111, 200, 220, 311 planes similar to X-ray diffraction analysis. The synthesized nanoparticles are evaluated for antimicrobial activity against 7 bacterial and 3 fungal pathogens. A good zone of inhibition was observed against pathogenic bacteria than fungal pathogens. Thus the study could hold a key aspect in drug discovery research and other pharmacological conducts of human clinical conditions.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.58-58
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• 2022

본 연구는 국내 6곳의 풍혈의 기능과 식물분포를 통하여 풍혈의 보전대책과 관리방안을 위한 기초자료를 확보하기 위해 진행하였다. 조사는 홍천 방내리, 정선 여탄리, 정선 운치리, 연천 동막리, 제천 금수산, 보은 구병산에 위치하는 풍혈 6곳을 선정하였고 풍혈에 기상측정장비를 설치하여 2021년 3월부터 2022년 3월까지 온도와 습도의 미기상요인을 측정하였다. 풍혈이 위치하는 지역의 미기상요인은 기상청 자료를 활용하였고 측정된 풍혈의 미기상요인과 비교 분석하였으며, 관속식물상은 계절별로 진행하여 각 풍혈에 서식하는 희귀식물과 북방계식물, 기후변화 취약식물을 분석하였다. 풍혈 6곳의 미기상요인 분석결과 온도는 보은 구병산 풍혈을 제외한 5곳의 풍혈은 여름철 냉혈의 기능을 나타냈고, 보은 구병산 풍혈은 겨울철 온혈의 기능을 하는 것으로 나타났으며, 습도는 6곳 모두 주변 지역보다 높게 나타났다. 멸종위기종 2급은 백부자, 산작약, 연잎꿩의다리 3분류군, 희귀식물은 산개나리, 월귤 등 23분류군, 북방계식물은 과남풀, 꽃개회나무 등 129분류군, 기후변화취약식물은 검종덩굴, 도깨비부채 등 23분류군으로 확인되었다. 풍혈은 기후변화 취약식물의 피난처로서 역할을 하는 것으로 나타났으나 산림유전자원보호구역으로 지정된 홍천 방내리 풍혈을 제외한 나머지 풍혈은 관리가 미흡하다. 정선 여탄리 풍혈은 인근 도로에서의 외래식물의 유입, 정선 운치리 풍혈은 인근의 양봉장과 관광객 등의 인간활동에 의한 훼손, 연천 동막리 풍혈은 인근 경작지 등의 사유지로 인한 관리의 어려움, 제천 금수산 풍혈은 관광지화로 인한 훼손, 보은 구병산 풍혈은 등산객의 답압으로 인한 훼손이 진행되고 있는 것으로 나타났다. 이에 따라 풍혈과 희귀 및 기후변화 취약식물들의 정기적인 모니터링과 풍혈 주변의 팬스 설치 등의 적극적인 보전방안이 필요하다고 판단된다.

• 한국환경생태학회지
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• 제29권4호
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• pp.570-579
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• 2015

녹나무과 생강나무속인 털조장나무는 한국 희귀식물 가운데 약관심종(LC), 식물구계학적 특정식물 IV등급 종이며 무등산국립공원 깃대종이다. 무등산, 천봉산 및 조계산 지역에서 털조장나무는 경사도는 $12^{\circ}{\sim}51^{\circ}$, 분포사면은 북서사면에서 북동사면까지, 해발고는 220~533m사이에 분포하였다. 털조장나무를 조사한 방형구에서 확인한 관속식물은 37과 54속 66종 5변종 1품종 총 72분류군이었다. 이중 식물구계학적 특정식물 IV등급종은 털조장나무, III등급 종에는 노각나무와 단풍나무가 있었으며, 한국 고유종은 노각나무와 지리대사초였다. 털조장나무가 분포하는 무등산지역의 식생군집은 신갈나무와 졸참나무-털조장나무, 때죽나무 군집, 천봉산지역은 노각나무-털조장나무, 조릿대군집, 조계산지역의 선암사지역은 서어나무-털조장나무, 조릿대군집 그리고 송광사 지역은 노각나무-털조장나무, 조릿대군집이었다. 조사된 털조장나무는 총 662개체였는데 이중 치수를 포함한 줄기가 1개인 개체는 353개(53.32%)였고, 2~5개의 맹아지를 갖는 개체는 270개(40.79%)였다. 맹아지 수가 가장 많은 개체는 27개로 확인되었다. 전체 662개체에서 맹아지 수는 1,198줄기였다. 또한 수고는 50cm에서 150cm까지의 개체가 699개(58.34%)로 가장 많았고 가장 큰 맹아지는 565cm였다. 맹아지의 근원 직경은 1cm미만이 992개(82.81%)로 가장 많았고 다음은 직경 1.0~1.5cm는 156개(13.2%)였다. 가장 큰 줄기는 근원 직경이 3.2cm이고 키는 565cm이었는데 근원부는 50%가, 흉고부는 25%가 고사된 상태였고, 줄기 한쪽이 세로로 찢어져 있었다. 4m이상인 3개체의 줄기도 정단부의 가지가 일부 고사된 상태였다. 따라서 한국산 털조장나무는 정아우세인 수형을 유지하지만 일정 수고 이상의 줄기는 고사되고 맹아지 수를 증가시킴으로서 개체의 형태적 적응을 보이는 관목이다.